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The map label for this gene is mvaB [H]

Identifier: 156976702

GI number: 156976702

Start: 688819

End: 689715

Strand: Reverse

Name: mvaB [H]

Synonym: VIBHAR_05477

Alternate gene names: 156976702

Gene position: 689715-688819 (Counterclockwise)

Preceding gene: 156976703

Following gene: 156976698

Centisome position: 31.29

GC content: 49.94

Gene sequence:

>897_bases
ATGAATTTGCCAAACAAGGTAAATATTGTCGAAGTGGGTGCGAGAGACGGATTACAAAACGAATCGCCAGTGTCCACGCA
AGCCAAGATCCGCTTAATCGACCTGCTCTCGGATACAGGGCTCACCCATATTGAGGCAGGCTCTTTTGTGTCGCCTAAAT
GGGTACCTCAAATGGCCGATTCACTCGATGTGATGAAGAGAATCACCCGCCGCCACAACGTCACCTACTCAGCGTTAACG
CCTAATGTGCAAGGCTTTGAGAAAGCGCTAGAAGCTGGAGCTACCCACGTTGCTATCTTCACCTCCTCATCGGAGGGCTT
TTGCAGACACAACATCAATTGCTCGATTGCGGAAAGCCTCACCCGCTTTGAGCCGGTAATGGAATTGGCAGTAACCCATA
ACATTCCCGTTCGGGGCTACTTATCTTGCGTGGCAGACTGCCCTTACGATGGATCAACCAAACCAGAACAAGTGGCAAGA
GTCGCTAACCTGCTGATGGAATTAGGCTGCTATGAGGTTTCACTTGGAGACACCATAGGTACAGGCACGCCTATGCGCAT
CGCTAAAATGTTAGAAGCCGTACTAGTGAAAGTCCCAACCCATAAGCTCGCTGTGCATTTTCACGATACGTGGGGACAAG
CGTTAGCAAATATCTACCAAGCCTTAACAATGGGTGTCCATACCGTCGATAGTAGTGTGGCTGGACTTGGGGGCTGTCCT
TACGCTCATGGCGCATCGGGTAATGTTGCTACCGAAGATGTGCTCTACCTTTGCCAAGGTTTAGGAATCGAGACAGGAGT
AGATTTGGAGTTGCTCGCCAAAGCCGGATGGATGATCAGTGAAGAGCTAAACCGTCAGCCGACCTCGAAGGTATCGATGG
CATTAAAGCAACGTTAA

Upstream 100 bases:

>100_bases
TCGCCGATATTCGCGTATCACCGCAAGGACAAGACGGCCTACAAGCCTTTCTGGAGAAACGAGAACCGGGTTGGATAAAC
AAGCCAGCTAAGGGCAAGAA

Downstream 100 bases:

>100_bases
TCATAAAACAAAAAGTTCTTTGTAAAACAAAAAGCTGCCTTGCTTAACAAGGCAGCTTTGTCATTTTCAAGTACCGGCGT
TTTTCAAGGTAGTTGTCATC

Product: hydroxymethylglutaryl-CoA lyase

Products: NA

Alternate protein names: HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MNLPNKVNIVEVGARDGLQNESPVSTQAKIRLIDLLSDTGLTHIEAGSFVSPKWVPQMADSLDVMKRITRRHNVTYSALT
PNVQGFEKALEAGATHVAIFTSSSEGFCRHNINCSIAESLTRFEPVMELAVTHNIPVRGYLSCVADCPYDGSTKPEQVAR
VANLLMELGCYEVSLGDTIGTGTPMRIAKMLEAVLVKVPTHKLAVHFHDTWGQALANIYQALTMGVHTVDSSVAGLGGCP
YAHGASGNVATEDVLYLCQGLGIETGVDLELLAKAGWMISEELNRQPTSKVSMALKQR

Sequences:

>Translated_298_residues
MNLPNKVNIVEVGARDGLQNESPVSTQAKIRLIDLLSDTGLTHIEAGSFVSPKWVPQMADSLDVMKRITRRHNVTYSALT
PNVQGFEKALEAGATHVAIFTSSSEGFCRHNINCSIAESLTRFEPVMELAVTHNIPVRGYLSCVADCPYDGSTKPEQVAR
VANLLMELGCYEVSLGDTIGTGTPMRIAKMLEAVLVKVPTHKLAVHFHDTWGQALANIYQALTMGVHTVDSSVAGLGGCP
YAHGASGNVATEDVLYLCQGLGIETGVDLELLAKAGWMISEELNRQPTSKVSMALKQR
>Mature_298_residues
MNLPNKVNIVEVGARDGLQNESPVSTQAKIRLIDLLSDTGLTHIEAGSFVSPKWVPQMADSLDVMKRITRRHNVTYSALT
PNVQGFEKALEAGATHVAIFTSSSEGFCRHNINCSIAESLTRFEPVMELAVTHNIPVRGYLSCVADCPYDGSTKPEQVAR
VANLLMELGCYEVSLGDTIGTGTPMRIAKMLEAVLVKVPTHKLAVHFHDTWGQALANIYQALTMGVHTVDSSVAGLGGCP
YAHGASGNVATEDVLYLCQGLGIETGVDLELLAKAGWMISEELNRQPTSKVSMALKQR

Specific function: Involved in the catabolism of branched amino acids such as leucine (Probable) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA lyase family [H]

Homologues:

Organism=Homo sapiens, GI62198232, Length=293, Percent_Identity=58.0204778156997, Blast_Score=348, Evalue=3e-96,
Organism=Homo sapiens, GI109150422, Length=293, Percent_Identity=55.9726962457338, Blast_Score=332, Evalue=2e-91,
Organism=Homo sapiens, GI109150427, Length=293, Percent_Identity=55.9726962457338, Blast_Score=331, Evalue=4e-91,
Organism=Homo sapiens, GI260654708, Length=136, Percent_Identity=61.0294117647059, Blast_Score=167, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI17510563, Length=293, Percent_Identity=49.8293515358362, Blast_Score=300, Evalue=5e-82,
Organism=Drosophila melanogaster, GI24582381, Length=293, Percent_Identity=54.9488054607508, Blast_Score=328, Evalue=2e-90,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000138
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =4.1.3.4 [H]

Molecular weight: Translated: 32147; Mature: 32147

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS01062 HMG_COA_LYASE ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLPNKVNIVEVGARDGLQNESPVSTQAKIRLIDLLSDTGLTHIEAGSFVSPKWVPQMAD
CCCCCCCCEEEECCCCCCCCCCCCCCHHHEEEEEHHHCCCCCEECCCCCCCCCHHHHHHH
SLDVMKRITRRHNVTYSALTPNVQGFEKALEAGATHVAIFTSSSEGFCRHNINCSIAESL
HHHHHHHHHHHHCCEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCHHHHHH
TRFEPVMELAVTHNIPVRGYLSCVADCPYDGSTKPEQVARVANLLMELGCYEVSLGDTIG
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCC
TGTPMRIAKMLEAVLVKVPTHKLAVHFHDTWGQALANIYQALTMGVHTVDSSVAGLGGCP
CCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
YAHGASGNVATEDVLYLCQGLGIETGVDLELLAKAGWMISEELNRQPTSKVSMALKQR
CCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MNLPNKVNIVEVGARDGLQNESPVSTQAKIRLIDLLSDTGLTHIEAGSFVSPKWVPQMAD
CCCCCCCCEEEECCCCCCCCCCCCCCHHHEEEEEHHHCCCCCEECCCCCCCCCHHHHHHH
SLDVMKRITRRHNVTYSALTPNVQGFEKALEAGATHVAIFTSSSEGFCRHNINCSIAESL
HHHHHHHHHHHHCCEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCHHHHHH
TRFEPVMELAVTHNIPVRGYLSCVADCPYDGSTKPEQVARVANLLMELGCYEVSLGDTIG
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCC
TGTPMRIAKMLEAVLVKVPTHKLAVHFHDTWGQALANIYQALTMGVHTVDSSVAGLGGCP
CCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
YAHGASGNVATEDVLYLCQGLGIETGVDLELLAKAGWMISEELNRQPTSKVSMALKQR
CCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2687236; 2656635; 1637819 [H]