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The map label for this gene is napA

Identifier: 156976603

GI number: 156976603

Start: 603071

End: 605560

Strand: Reverse

Name: napA

Synonym: VIBHAR_05377

Alternate gene names: 156976603

Gene position: 605560-603071 (Counterclockwise)

Preceding gene: 156976604

Following gene: 156976602

Centisome position: 27.48

GC content: 47.91

Gene sequence:

>2490_bases
ATGAAAATGACAAGACGTGCGTTTGTGAAAGCAAACGCGGCTGCATCAGCTGCTGCTGTCGCAGGTATTACACTACCAGC
TTCTGCAACCAATCTGATTGCGAGCTCTGATCAAACCAAAATAACATGGGACAAAGCGCCTTGTCGTTTCTGTGGTACAG
GCTGTTCTGTTCTTGTTGGTACACAAAACGGCAAAGTGGTAGCAACTCAGGGTGACCCTGAAGCACCAGTAAACAAAGGC
CTTAACTGTATCAAAGGTTACTTCCTTTCTAAGATCATGTACGGTCAAGATCGCCTAACTAAGCCTCTTCTACGTATGAA
AGACGGCAAATACAGCAAAGACGGCGACTTCGCTCCTGTATCTTGGGATACCGCTTTCGACATCATGGCTGAAAAATGGA
AAGCGTCTTTAGAGAAAAAAGGCCCTACAAGCATCGGTATGTTCGGTTCTGGTCAATGGACCGTAATGGAAGGTTATGCC
GCTGCCAAAATGATGAAAGCAGGCTTCCGTTCAAACAACATCGACCCGAACGCGCGTCACTGTATGGCGTCTGCGGTAGT
AGGCTTCATGCGTGCATTTGGTATCGATGAACCAATGGGCTGTTACGATGACTTCGAGAACGCAGATGCCTTCGTTCTTT
GGGGTTCAAACATGGCAGAAATGCACCCAGTTCTTTGGACTCGTATTACTGACCGTCGTCTAAGTCACCCTCACGTTAAA
GTGAACGTGCTTTCTACTTACTACCACCGTTCATTTGAGTTGGCAGACCACGGCTACATTTTCAATCCTCAGTCTGACCT
TGCGATTGCTAACTTCATCGCAAACTACATCATCGAAAACGATGCAGTAAACTGGGACTTCGTGAACAAGCACACGAACT
TCACTCAAGCAGACACGGACATCGGCTACGGTCTACGTGACGATGATCCGCTACAAAAAGCAGCGAAGAACCCGAACTCA
GGCAAACTAACTTCTATCTCTTTCGAAGAGTACAAGAAGTCAGTAGCACCATACACAGTAGAGAAAGCGTCTGAAATCTC
TGGTGTTGAGAAAGAGAAACTGATTGAGCTTGCTAAACAATACGCGGATCCAAACACGAAAGTGATGTCTCTTTGGACGA
TGGGTATGAACCAACATACTCGTGGTGTATGGATGAACAATTTGATTTACAACATCCACCTACTAACCGGTAAGATTGCG
ACTCCGGGTAACAGCCCATTCTCACTAACAGGTCAGCCATCAGCGTGTGGTACGGCTCGTGAAGTTGGTACGTTTGCTCA
CCGTCTACCAGCAGACATGGTGGTTGCTAACCCTAAACACCGTAAGATCGCAGAAGACATCTGGAAACTACCAGAAGGTA
CTATTCCACCAAAACCTGGCTTCCACGCAGTACTTCAAGACCGTATGTTGCATGACGGCGTTCTGAACTGTTACTGGGTT
CAATGTAACAACAACATGCAAGCAGGTCCGAACATCAACACTGAACGTCTTCCTGGTTACCGTAACCCAGAAAACTTCAT
CGTTGTATCTGACCCATACCCAACGGCAACAGCGCAAGCCGCTGACCTTGTTCTTCCGACCGCAATGTGGATTGAGAAAG
AAGGTGCTTACGGTAACGCAGAGCGTCGTACTCAGGCTTGGTACCAACAAGTTGAGACTATCGGCGATGCGAAATCTGAC
CTATGGCAGGTAATGGAATTTGCAAAACGCTTCAAGATGGAAGAAGTGTGGCCAGAAGAGCTACTTGCAAAAGCACCTGA
ATACCGCGGTAAGACAATGTACGACATGCTGTTCAAGAACGGTCAGGTCGACAAGTTCCCTATCGAAGAAGCGCGTGAGA
TGAACGATGACGCTCACCACTTCGGCTACTACGTACAGAAAGGTCTGTTCGAAGAGTACGCGACGTTTGGTCGTGGTCAC
GGTCACGACTTAGCACCATACGATGTTTACCACACGGTACGTGGTCTACGTTGGCCGGTTGTTGATGGCAAAGAAACACA
ATGGCGCTTTAAAGAAGGCTCTGACCCGTACGCGAAAGCAGGTTCAGGTTGGGATTTCTACGGCAATGCTGATGGTAAAG
CGAAGATTATCTCTGCACCATACGAAGCGCCACCAGAAGTGCCAAACGAAGAGTTTGACCTATGGCTATGTACTGGCCGT
GTTCTTGAGCACTGGCACACTGGTACTATGACTCGTCGTGTACCAGAGCTTTACAAAGCGGTGCCTGACGCGGTGGTTTA
CATGCACCCTGCGGATGCGAAGAAACGCAACGTACGCCGTGGTGAAGAAGTTCTGATTGCAAACAAACGTGGTGAAGTAC
GCGTTCGCGTTGAAACTCGCGGCCGTAACCGTCCACCTGAAGGCTTGGTATTCGTACCGTTCTTTGATGCCCGTATTTTG
ATTAACAAGTTGATCCTTGATGCGACTGACCCTCTGTCTAAACAGACAGACTTTAAGAAGTGTCCAGTCAAAATCACGAA
AATTGCTTAA

Upstream 100 bases:

>100_bases
TCTTGAGTACAGTACTGGTTTATCACCAAATCGAAACTGGGCTTGAAGAAACGGAAGAAAAAGACACTGGAATACAACAT
TCCCAAATCGAGGGTGAAGT

Downstream 100 bases:

>100_bases
CCGTCAAATAATAAATGCAAAGGCCGTCTACTCGGCGGCCTAAAAAGAATTTAAGCCATAAGGCGGAGATGAAATGATGA
AAAAATACCTTATTGCACTT

Product: nitrate reductase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 829; Mature: 829

Protein sequence:

>829_residues
MKMTRRAFVKANAAASAAAVAGITLPASATNLIASSDQTKITWDKAPCRFCGTGCSVLVGTQNGKVVATQGDPEAPVNKG
LNCIKGYFLSKIMYGQDRLTKPLLRMKDGKYSKDGDFAPVSWDTAFDIMAEKWKASLEKKGPTSIGMFGSGQWTVMEGYA
AAKMMKAGFRSNNIDPNARHCMASAVVGFMRAFGIDEPMGCYDDFENADAFVLWGSNMAEMHPVLWTRITDRRLSHPHVK
VNVLSTYYHRSFELADHGYIFNPQSDLAIANFIANYIIENDAVNWDFVNKHTNFTQADTDIGYGLRDDDPLQKAAKNPNS
GKLTSISFEEYKKSVAPYTVEKASEISGVEKEKLIELAKQYADPNTKVMSLWTMGMNQHTRGVWMNNLIYNIHLLTGKIA
TPGNSPFSLTGQPSACGTAREVGTFAHRLPADMVVANPKHRKIAEDIWKLPEGTIPPKPGFHAVLQDRMLHDGVLNCYWV
QCNNNMQAGPNINTERLPGYRNPENFIVVSDPYPTATAQAADLVLPTAMWIEKEGAYGNAERRTQAWYQQVETIGDAKSD
LWQVMEFAKRFKMEEVWPEELLAKAPEYRGKTMYDMLFKNGQVDKFPIEEAREMNDDAHHFGYYVQKGLFEEYATFGRGH
GHDLAPYDVYHTVRGLRWPVVDGKETQWRFKEGSDPYAKAGSGWDFYGNADGKAKIISAPYEAPPEVPNEEFDLWLCTGR
VLEHWHTGTMTRRVPELYKAVPDAVVYMHPADAKKRNVRRGEEVLIANKRGEVRVRVETRGRNRPPEGLVFVPFFDARIL
INKLILDATDPLSKQTDFKKCPVKITKIA

Sequences:

>Translated_829_residues
MKMTRRAFVKANAAASAAAVAGITLPASATNLIASSDQTKITWDKAPCRFCGTGCSVLVGTQNGKVVATQGDPEAPVNKG
LNCIKGYFLSKIMYGQDRLTKPLLRMKDGKYSKDGDFAPVSWDTAFDIMAEKWKASLEKKGPTSIGMFGSGQWTVMEGYA
AAKMMKAGFRSNNIDPNARHCMASAVVGFMRAFGIDEPMGCYDDFENADAFVLWGSNMAEMHPVLWTRITDRRLSHPHVK
VNVLSTYYHRSFELADHGYIFNPQSDLAIANFIANYIIENDAVNWDFVNKHTNFTQADTDIGYGLRDDDPLQKAAKNPNS
GKLTSISFEEYKKSVAPYTVEKASEISGVEKEKLIELAKQYADPNTKVMSLWTMGMNQHTRGVWMNNLIYNIHLLTGKIA
TPGNSPFSLTGQPSACGTAREVGTFAHRLPADMVVANPKHRKIAEDIWKLPEGTIPPKPGFHAVLQDRMLHDGVLNCYWV
QCNNNMQAGPNINTERLPGYRNPENFIVVSDPYPTATAQAADLVLPTAMWIEKEGAYGNAERRTQAWYQQVETIGDAKSD
LWQVMEFAKRFKMEEVWPEELLAKAPEYRGKTMYDMLFKNGQVDKFPIEEAREMNDDAHHFGYYVQKGLFEEYATFGRGH
GHDLAPYDVYHTVRGLRWPVVDGKETQWRFKEGSDPYAKAGSGWDFYGNADGKAKIISAPYEAPPEVPNEEFDLWLCTGR
VLEHWHTGTMTRRVPELYKAVPDAVVYMHPADAKKRNVRRGEEVLIANKRGEVRVRVETRGRNRPPEGLVFVPFFDARIL
INKLILDATDPLSKQTDFKKCPVKITKIA
>Mature_829_residues
MKMTRRAFVKANAAASAAAVAGITLPASATNLIASSDQTKITWDKAPCRFCGTGCSVLVGTQNGKVVATQGDPEAPVNKG
LNCIKGYFLSKIMYGQDRLTKPLLRMKDGKYSKDGDFAPVSWDTAFDIMAEKWKASLEKKGPTSIGMFGSGQWTVMEGYA
AAKMMKAGFRSNNIDPNARHCMASAVVGFMRAFGIDEPMGCYDDFENADAFVLWGSNMAEMHPVLWTRITDRRLSHPHVK
VNVLSTYYHRSFELADHGYIFNPQSDLAIANFIANYIIENDAVNWDFVNKHTNFTQADTDIGYGLRDDDPLQKAAKNPNS
GKLTSISFEEYKKSVAPYTVEKASEISGVEKEKLIELAKQYADPNTKVMSLWTMGMNQHTRGVWMNNLIYNIHLLTGKIA
TPGNSPFSLTGQPSACGTAREVGTFAHRLPADMVVANPKHRKIAEDIWKLPEGTIPPKPGFHAVLQDRMLHDGVLNCYWV
QCNNNMQAGPNINTERLPGYRNPENFIVVSDPYPTATAQAADLVLPTAMWIEKEGAYGNAERRTQAWYQQVETIGDAKSD
LWQVMEFAKRFKMEEVWPEELLAKAPEYRGKTMYDMLFKNGQVDKFPIEEAREMNDDAHHFGYYVQKGLFEEYATFGRGH
GHDLAPYDVYHTVRGLRWPVVDGKETQWRFKEGSDPYAKAGSGWDFYGNADGKAKIISAPYEAPPEVPNEEFDLWLCTGR
VLEHWHTGTMTRRVPELYKAVPDAVVYMHPADAKKRNVRRGEEVLIANKRGEVRVRVETRGRNRPPEGLVFVPFFDARIL
INKLILDATDPLSKQTDFKKCPVKITKIA

Specific function: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and peripla

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily

Homologues:

Organism=Escherichia coli, GI1788534, Length=830, Percent_Identity=69.5180722891566, Blast_Score=1242, Evalue=0.0,
Organism=Escherichia coli, GI3868721, Length=795, Percent_Identity=24.1509433962264, Blast_Score=210, Evalue=3e-55,
Organism=Escherichia coli, GI3868720, Length=661, Percent_Identity=22.8441754916793, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI87081797, Length=867, Percent_Identity=21.9146482122261, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI1787870, Length=832, Percent_Identity=22.5961538461538, Blast_Score=100, Evalue=5e-22,
Organism=Escherichia coli, GI3868719, Length=664, Percent_Identity=21.5361445783133, Blast_Score=96, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAPA_VIBHB (A7N7J3)

Other databases:

- EMBL:   CP000790
- RefSeq:   YP_001447509.1
- ProteinModelPortal:   A7N7J3
- SMR:   A7N7J3
- STRING:   A7N7J3
- GeneID:   5557405
- GenomeReviews:   CP000790_GR
- KEGG:   vha:VIBHAR_05377
- NMPDR:   fig|338187.4.peg.3153
- eggNOG:   COG0243
- HOGENOM:   HBG715033
- OMA:   IISAPYE
- ProtClustDB:   PRK13532
- GO:   GO:0006810
- HAMAP:   MF_01630
- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR010051
- InterPro:   IPR006311
- Gene3D:   G3DSA:2.40.40.20
- SMART:   SM00926
- TIGRFAMs:   TIGR01706
- TIGRFAMs:   TIGR01409

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding; SSF50692 Asp_decarb_fold

EC number: =1.7.99.4

Molecular weight: Translated: 93062; Mature: 93062

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1; PS00490 MOLYBDOPTERIN_PROK_2; PS00932 MOLYBDOPTERIN_PROK_3; PS51318 TAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMTRRAFVKANAAASAAAVAGITLPASATNLIASSDQTKITWDKAPCRFCGTGCSVLVG
CCCCHHHHHHCCCHHHHHHHEEEECCCCHHHHEECCCCCEEEECCCCCCEECCCCEEEEE
TQNGKVVATQGDPEAPVNKGLNCIKGYFLSKIMYGQDRLTKPLLRMKDGKYSKDGDFAPV
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCC
SWDTAFDIMAEKWKASLEKKGPTSIGMFGSGQWTVMEGYAAAKMMKAGFRSNNIDPNARH
CCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECHHHHHHHHHHCCCCCCCCCHHHH
CMASAVVGFMRAFGIDEPMGCYDDFENADAFVLWGSNMAEMHPVLWTRITDRRLSHPHVK
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHCHHHHHHHHHCCCCCCCEE
VNVLSTYYHRSFELADHGYIFNPQSDLAIANFIANYIIENDAVNWDFVNKHTNFTQADTD
EEEEEHHHHCEEEECCCCEEECCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCC
IGYGLRDDDPLQKAAKNPNSGKLTSISFEEYKKSVAPYTVEKASEISGVEKEKLIELAKQ
CCCCCCCCCHHHHHHCCCCCCCEEEEEHHHHHHHCCCEEHHHHHHHCCCCHHHHHHHHHH
YADPNTKVMSLWTMGMNQHTRGVWMNNLIYNIHLLTGKIATPGNSPFSLTGQPSACGTAR
HCCCCCEEEEEEECCCCCCCCCEEECCEEEEEEEEEEEEECCCCCCEEECCCCCCCCCHH
EVGTFAHRLPADMVVANPKHRKIAEDIWKLPEGTIPPKPGFHAVLQDRMLHDGVLNCYWV
HHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEE
QCNNNMQAGPNINTERLPGYRNPENFIVVSDPYPTATAQAADLVLPTAMWIEKEGAYGNA
EECCCCCCCCCCCHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHCCHHHEEECCCCCCCH
ERRTQAWYQQVETIGDAKSDLWQVMEFAKRFKMEEVWPEELLAKAPEYRGKTMYDMLFKN
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHCCHHHHHHCCCCCCCCHHHHHHHCC
GQVDKFPIEEAREMNDDAHHFGYYVQKGLFEEYATFGRGHGHDLAPYDVYHTVRGLRWPV
CCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCC
VDGKETQWRFKEGSDPYAKAGSGWDFYGNADGKAKIISAPYEAPPEVPNEEFDLWLCTGR
CCCCCCCEEECCCCCHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEHH
VLEHWHTGTMTRRVPELYKAVPDAVVYMHPADAKKRNVRRGEEVLIANKRGEVRVRVETR
HHHHHCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHCCCCCEEEEECCCCCEEEEEEEC
GRNRPPEGLVFVPFFDARILINKLILDATDPLSKQTDFKKCPVKITKIA
CCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MKMTRRAFVKANAAASAAAVAGITLPASATNLIASSDQTKITWDKAPCRFCGTGCSVLVG
CCCCHHHHHHCCCHHHHHHHEEEECCCCHHHHEECCCCCEEEECCCCCCEECCCCEEEEE
TQNGKVVATQGDPEAPVNKGLNCIKGYFLSKIMYGQDRLTKPLLRMKDGKYSKDGDFAPV
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCC
SWDTAFDIMAEKWKASLEKKGPTSIGMFGSGQWTVMEGYAAAKMMKAGFRSNNIDPNARH
CCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECHHHHHHHHHHCCCCCCCCCHHHH
CMASAVVGFMRAFGIDEPMGCYDDFENADAFVLWGSNMAEMHPVLWTRITDRRLSHPHVK
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHCHHHHHHHHHCCCCCCCEE
VNVLSTYYHRSFELADHGYIFNPQSDLAIANFIANYIIENDAVNWDFVNKHTNFTQADTD
EEEEEHHHHCEEEECCCCEEECCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCC
IGYGLRDDDPLQKAAKNPNSGKLTSISFEEYKKSVAPYTVEKASEISGVEKEKLIELAKQ
CCCCCCCCCHHHHHHCCCCCCCEEEEEHHHHHHHCCCEEHHHHHHHCCCCHHHHHHHHHH
YADPNTKVMSLWTMGMNQHTRGVWMNNLIYNIHLLTGKIATPGNSPFSLTGQPSACGTAR
HCCCCCEEEEEEECCCCCCCCCEEECCEEEEEEEEEEEEECCCCCCEEECCCCCCCCCHH
EVGTFAHRLPADMVVANPKHRKIAEDIWKLPEGTIPPKPGFHAVLQDRMLHDGVLNCYWV
HHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEE
QCNNNMQAGPNINTERLPGYRNPENFIVVSDPYPTATAQAADLVLPTAMWIEKEGAYGNA
EECCCCCCCCCCCHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHCCHHHEEECCCCCCCH
ERRTQAWYQQVETIGDAKSDLWQVMEFAKRFKMEEVWPEELLAKAPEYRGKTMYDMLFKN
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHCCHHHHHHCCCCCCCCHHHHHHHCC
GQVDKFPIEEAREMNDDAHHFGYYVQKGLFEEYATFGRGHGHDLAPYDVYHTVRGLRWPV
CCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCC
VDGKETQWRFKEGSDPYAKAGSGWDFYGNADGKAKIISAPYEAPPEVPNEEFDLWLCTGR
CCCCCCCEEECCCCCHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEHH
VLEHWHTGTMTRRVPELYKAVPDAVVYMHPADAKKRNVRRGEEVLIANKRGEVRVRVETR
HHHHHCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHCCCCCEEEEECCCCCEEEEEEEC
GRNRPPEGLVFVPFFDARILINKLILDATDPLSKQTDFKKCPVKITKIA
CCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA