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The map label for this gene is napB [H]

Identifier: 156976602

GI number: 156976602

Start: 602539

End: 602997

Strand: Reverse

Name: napB [H]

Synonym: VIBHAR_05376

Alternate gene names: 156976602

Gene position: 602997-602539 (Counterclockwise)

Preceding gene: 156976603

Following gene: 156976601

Centisome position: 27.36

GC content: 45.53

Gene sequence:

>459_bases
ATGATGAAAAAATACCTTATTGCACTTTTATCTGTAGGTGCTTTATTGACAGGCGTTGCACAAGCAGAACTGGATAACCC
AGGCGGCACAGGCGGTCTCGACTCGCTACGTGGCAGCGCGCAACTTGAAGACACGCGTCCTGCAGATGATTTTAAAGACT
TTCCAAAAGACCAAATCGTTGCAGACAGCTATGTATATCAGCCACCACTGATCCCACACAGCATCCGAAATTACGAAGTG
TCTTTGAACGCAAACAAATGTCTGGCATGTCACAGCTGGAAAAATGCTAAATACGCAGGCGCTACCAAGATCAGTGTAAC
GCACTACGTTAACCGTGAAGACGCGGTATTGGCAGATATGTCACCACGTCGTTACTTCTGTTTACAATGTCACGTTCCAC
AAACCGATGGTAAACCATTAGTGGAAAACGAGTTTAAGGCAGTCGATTCGCTTCATTAA

Upstream 100 bases:

>100_bases
CCAGTCAAAATCACGAAAATTGCTTAACCGTCAAATAATAAATGCAAAGGCCGTCTACTCGGCGGCCTAAAAAGAATTTA
AGCCATAAGGCGGAGATGAA

Downstream 100 bases:

>100_bases
TTGAAACGCGAGTACTAAGAGGTTACTTATGAAAATACTTAAAGCGTTTTGGAAGAGACTGTCGAGCCCGAGCAAAGCTG
CCGCTGGTGTAGTTTTGTTC

Product: cytochrome c-type protein NapB

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 152; Mature: 152

Protein sequence:

>152_residues
MMKKYLIALLSVGALLTGVAQAELDNPGGTGGLDSLRGSAQLEDTRPADDFKDFPKDQIVADSYVYQPPLIPHSIRNYEV
SLNANKCLACHSWKNAKYAGATKISVTHYVNREDAVLADMSPRRYFCLQCHVPQTDGKPLVENEFKAVDSLH

Sequences:

>Translated_152_residues
MMKKYLIALLSVGALLTGVAQAELDNPGGTGGLDSLRGSAQLEDTRPADDFKDFPKDQIVADSYVYQPPLIPHSIRNYEV
SLNANKCLACHSWKNAKYAGATKISVTHYVNREDAVLADMSPRRYFCLQCHVPQTDGKPLVENEFKAVDSLH
>Mature_152_residues
MMKKYLIALLSVGALLTGVAQAELDNPGGTGGLDSLRGSAQLEDTRPADDFKDFPKDQIVADSYVYQPPLIPHSIRNYEV
SLNANKCLACHSWKNAKYAGATKISVTHYVNREDAVLADMSPRRYFCLQCHVPQTDGKPLVENEFKAVDSLH

Specific function: Small subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme NapC protein, thus allowing electron flow between membrane and periplasm.

COG id: COG3043

COG function: function code C; Nitrate reductase cytochrome c-type subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082065, Length=93, Percent_Identity=49.4623655913978, Blast_Score=106, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011031
- InterPro:   IPR005591 [H]

Pfam domain/function: PF03892 NapB [H]

EC number: NA

Molecular weight: Translated: 16767; Mature: 16767

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKKYLIALLSVGALLTGVAQAELDNPGGTGGLDSLRGSAQLEDTRPADDFKDFPKDQIV
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCCCCCHHHHCCCCCEE
ADSYVYQPPLIPHSIRNYEVSLNANKCLACHSWKNAKYAGATKISVTHYVNREDAVLADM
ECCEEECCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCEEEEEEEEECCCCCEEECC
SPRRYFCLQCHVPQTDGKPLVENEFKAVDSLH
CCCEEEEEEEECCCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MMKKYLIALLSVGALLTGVAQAELDNPGGTGGLDSLRGSAQLEDTRPADDFKDFPKDQIV
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCCCCCHHHHCCCCCEE
ADSYVYQPPLIPHSIRNYEVSLNANKCLACHSWKNAKYAGATKISVTHYVNREDAVLADM
ECCEEECCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCEEEEEEEEECCCCCEEECC
SPRRYFCLQCHVPQTDGKPLVENEFKAVDSLH
CCCEEEEEEEECCCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8730872 [H]