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The map label for this gene is rhtB [H]

Identifier: 156976590

GI number: 156976590

Start: 593572

End: 594192

Strand: Reverse

Name: rhtB [H]

Synonym: VIBHAR_05364

Alternate gene names: 156976590

Gene position: 594192-593572 (Counterclockwise)

Preceding gene: 156976595

Following gene: 156976589

Centisome position: 26.96

GC content: 47.99

Gene sequence:

>621_bases
ATGAATACCGCTTTGCTAACCATGTTCATCCCTACCTTCTTTTTTGTTTCGATCACGCCTGGGATGTGTATGACCCTTGC
TCTCACGTTAGGAATGAGTATTGGGTATCGCCGAACCCTATGGATGATGGCTGGTGAACTGGTTGGCGTGGCACTGGTGT
CTGTCTCTGCGGTATTGGGCATCGCTGCAATAATGCTGAATTATCCTTGGTTATTCACCGCCCTTAAATTAATTGGTGGT
GGCTACCTTCTCTATTTAGGCATCGAAATGTGGCGCTCACGTGGCAAACTCGCCATCAACGTTGAGAATGTTGGTGTCCC
GACTAGTGGCAACTGGGATTTGGTTTTACAGGGCTTTGTCACCGCAATCGCCAACCCTAAAGGTTGGGCGTTTATGATTC
CCCTTCTGCCACCTTTTATCAATCAAAATGCCGCTCTTGCGCCTCAGCTGATCGTACTTGTTTCGATTATTCTGATGTTC
GAGTTCATTTGTATGTCGCTTTACGCCACAGGTGGCAAAGGCTTGAAGCGTTTGCTGGGGCAATCAAAAAACGTCCGCTT
GATGAACCGAATCGCTGGCACCTTGATGATGGGTGTGGGTGTATGGCTGTTGATGAGTTAA

Upstream 100 bases:

>100_bases
CCCTATGCTCCCCATGACTATTTTCTTTAAAGGTGGCATTGAGCGTCACTTTGCACTATCTTTGAGCCCTAACTCCTAAA
TTACATAATCATCAATATTC

Downstream 100 bases:

>100_bases
AGAAAGCGTTTTCGATAGAAAGTCCGAGTTATGAAGAAAATTTTAGATAAGCTCAAACAGGCCATCGGTGAGGTATTGGA
TTTCCATGCTCGAGTATGGG

Product: putative threonine efflux protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MNTALLTMFIPTFFFVSITPGMCMTLALTLGMSIGYRRTLWMMAGELVGVALVSVSAVLGIAAIMLNYPWLFTALKLIGG
GYLLYLGIEMWRSRGKLAINVENVGVPTSGNWDLVLQGFVTAIANPKGWAFMIPLLPPFINQNAALAPQLIVLVSIILMF
EFICMSLYATGGKGLKRLLGQSKNVRLMNRIAGTLMMGVGVWLLMS

Sequences:

>Translated_206_residues
MNTALLTMFIPTFFFVSITPGMCMTLALTLGMSIGYRRTLWMMAGELVGVALVSVSAVLGIAAIMLNYPWLFTALKLIGG
GYLLYLGIEMWRSRGKLAINVENVGVPTSGNWDLVLQGFVTAIANPKGWAFMIPLLPPFINQNAALAPQLIVLVSIILMF
EFICMSLYATGGKGLKRLLGQSKNVRLMNRIAGTLMMGVGVWLLMS
>Mature_206_residues
MNTALLTMFIPTFFFVSITPGMCMTLALTLGMSIGYRRTLWMMAGELVGVALVSVSAVLGIAAIMLNYPWLFTALKLIGG
GYLLYLGIEMWRSRGKLAINVENVGVPTSGNWDLVLQGFVTAIANPKGWAFMIPLLPPFINQNAALAPQLIVLVSIILMF
EFICMSLYATGGKGLKRLLGQSKNVRLMNRIAGTLMMGVGVWLLMS

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=192, Percent_Identity=28.6458333333333, Blast_Score=70, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22429; Mature: 22429

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
7.8 %Met     (Translated Protein)
8.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
7.8 %Met     (Mature Protein)
8.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTALLTMFIPTFFFVSITPGMCMTLALTLGMSIGYRRTLWMMAGELVGVALVSVSAVLG
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IAAIMLNYPWLFTALKLIGGGYLLYLGIEMWRSRGKLAINVENVGVPTSGNWDLVLQGFV
HHHHHHCCHHHHHHHHHHCCCHHEEHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHH
TAIANPKGWAFMIPLLPPFINQNAALAPQLIVLVSIILMFEFICMSLYATGGKGLKRLLG
HHHCCCCCCEEEECCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHC
QSKNVRLMNRIAGTLMMGVGVWLLMS
CCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNTALLTMFIPTFFFVSITPGMCMTLALTLGMSIGYRRTLWMMAGELVGVALVSVSAVLG
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IAAIMLNYPWLFTALKLIGGGYLLYLGIEMWRSRGKLAINVENVGVPTSGNWDLVLQGFV
HHHHHHCCHHHHHHHHHHCCCHHEEHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHH
TAIANPKGWAFMIPLLPPFINQNAALAPQLIVLVSIILMFEFICMSLYATGGKGLKRLLG
HHHCCCCCCEEEECCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHC
QSKNVRLMNRIAGTLMMGVGVWLLMS
CCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]