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The map label for this gene is b2657; [C]

Identifier: 156976480

GI number: 156976480

Start: 501532

End: 502881

Strand: Direct

Name: b2657; [C]

Synonym: VIBHAR_05253

Alternate gene names: 156976480

Gene position: 501532-502881 (Clockwise)

Preceding gene: 156976478

Following gene: 156976483

Centisome position: 22.76

GC content: 46.52

Gene sequence:

>1350_bases
ATGAGTGGCTTACAGGAAACACAATCAACAGGTGCAAATGTTATCTTGCACGCTTTTGATTGGAAGTATGCTGACATTGC
CCAACGCGCAGCGGAGATCAGCGAGCTAGGTTATGGCTCGGTATTGGTTTCTCCGCCGATGAAAAGCGCACAAGATGAAC
GCTGGTGGCAGCGTTACCAACCTCAAGATTATCGTGTTATCGACAATGCGCTCGGTAATACCACAGATTTTAAAAACATG
GTTGAAGCACTCGACCGTGTAGGTGTATTGGTTTACGCCGATGTCGTCTTTAATCACATGGCGAACGAAGCCCATCTACG
CTCGGATTTACAATATCCGCGCCAAGCCGTGATGGACGATTACCAACAAAATGCAAGCAAGTATCAAGATCTGATTCTAT
TCGGTGACTTGTCAGAACCGCTGTTTGATGAGAATGACTTCGTTGAAGCTTTTGGCATCAAAGATTGGAAAGACAAGTGG
GAAGTTCAAAATGGTCGAATCTCAGGCGGTCCAACCGATCCTGGGTTGCCGACTTTACGTGTATGCAAGCATGTTGTTGA
GCAGCAACGTACTTACCTGAAAGCGCTTAAGGCTCTGGGCGTGAAAGGTTTCCGTATTGATGCCGCAAAGCACATGACGT
TAGAACATCTAAAATTGGTTTGGACCGAAGACATCACTCAAGACGTCCATATCTTTGGTGAAATCATCACAGATGGTGGC
GCGACAGAAGAAGAATATGAGCTGTTCCTAGAACCGTATTTGAAAGAAACACGTTTAGGTGCATACGATTTTCCGTTGTT
CTCTACCATCTTTAAGGCGTTCTCTAAGAAGGGCAGTTTTAAGTCTTTGATCGACCCGTACTGTTTTGGTCAGGCTTTAT
CTAACCGTCGAGCTATTACTTTCGCGATTACTCATGATATTCCAAACAATGATGTGTTCTTGGATTTAGTCATGGATGAA
GAGAACGAATGGCTAGCGTATGCCTACATTCTGGGACGCGATGGCGGTGTTCCGCTTATTTACACGGATTTGGACACCAG
TGGTATCAAGGGCGTGGACGGTAAACCAAGATGGCAGAATGCGTGGAAGGACGCACGTATGGCAAAGGTGGTGGCATTCC
ATAATCTTGTTCATGGCGAGCCAATGACGGTACTAGAAGGTAACGACGACATGTTGGTACTTCAGCGCGGCGAGAAGGGC
ATCTTGGTACTCAACAAATCTGCGCGAGCGCAATCACTGCAGCTGACAGTGAACAAGGCTCTGGTTGATATGATGACCGG
AGAAGAAGTGCCTAGCGGTGAAGAGATTAAAGTGGCGGCGAAATCAGCAATGCTGCTTGTAGAAAAGTAA

Upstream 100 bases:

>100_bases
TGAGGGAGGTTCGCTAATAGTTCTTATAATTTGGGCTATTGGATTCGCTCACGAAGAATACGAGTAACAATGGGTGAGCG
AACGCGACGAGGAGAGAAAC

Downstream 100 bases:

>100_bases
TACCCCTTAGAAGCGCAAACAGCTTTGGAGTTTGCGCTTCTTTTTCCCTTATTTTGTCGCTTGGGATTTAGTCGACGAGT
TTAGCGACGATCTTTCCGAC

Product: alpha-amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MSGLQETQSTGANVILHAFDWKYADIAQRAAEISELGYGSVLVSPPMKSAQDERWWQRYQPQDYRVIDNALGNTTDFKNM
VEALDRVGVLVYADVVFNHMANEAHLRSDLQYPRQAVMDDYQQNASKYQDLILFGDLSEPLFDENDFVEAFGIKDWKDKW
EVQNGRISGGPTDPGLPTLRVCKHVVEQQRTYLKALKALGVKGFRIDAAKHMTLEHLKLVWTEDITQDVHIFGEIITDGG
ATEEEYELFLEPYLKETRLGAYDFPLFSTIFKAFSKKGSFKSLIDPYCFGQALSNRRAITFAITHDIPNNDVFLDLVMDE
ENEWLAYAYILGRDGGVPLIYTDLDTSGIKGVDGKPRWQNAWKDARMAKVVAFHNLVHGEPMTVLEGNDDMLVLQRGEKG
ILVLNKSARAQSLQLTVNKALVDMMTGEEVPSGEEIKVAAKSAMLLVEK

Sequences:

>Translated_449_residues
MSGLQETQSTGANVILHAFDWKYADIAQRAAEISELGYGSVLVSPPMKSAQDERWWQRYQPQDYRVIDNALGNTTDFKNM
VEALDRVGVLVYADVVFNHMANEAHLRSDLQYPRQAVMDDYQQNASKYQDLILFGDLSEPLFDENDFVEAFGIKDWKDKW
EVQNGRISGGPTDPGLPTLRVCKHVVEQQRTYLKALKALGVKGFRIDAAKHMTLEHLKLVWTEDITQDVHIFGEIITDGG
ATEEEYELFLEPYLKETRLGAYDFPLFSTIFKAFSKKGSFKSLIDPYCFGQALSNRRAITFAITHDIPNNDVFLDLVMDE
ENEWLAYAYILGRDGGVPLIYTDLDTSGIKGVDGKPRWQNAWKDARMAKVVAFHNLVHGEPMTVLEGNDDMLVLQRGEKG
ILVLNKSARAQSLQLTVNKALVDMMTGEEVPSGEEIKVAAKSAMLLVEK
>Mature_448_residues
SGLQETQSTGANVILHAFDWKYADIAQRAAEISELGYGSVLVSPPMKSAQDERWWQRYQPQDYRVIDNALGNTTDFKNMV
EALDRVGVLVYADVVFNHMANEAHLRSDLQYPRQAVMDDYQQNASKYQDLILFGDLSEPLFDENDFVEAFGIKDWKDKWE
VQNGRISGGPTDPGLPTLRVCKHVVEQQRTYLKALKALGVKGFRIDAAKHMTLEHLKLVWTEDITQDVHIFGEIITDGGA
TEEEYELFLEPYLKETRLGAYDFPLFSTIFKAFSKKGSFKSLIDPYCFGQALSNRRAITFAITHDIPNNDVFLDLVMDEE
NEWLAYAYILGRDGGVPLIYTDLDTSGIKGVDGKPRWQNAWKDARMAKVVAFHNLVHGEPMTVLEGNDDMLVLQRGEKGI
LVLNKSARAQSLQLTVNKALVDMMTGEEVPSGEEIKVAAKSAMLLVEK

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI56549664, Length=480, Percent_Identity=25.4166666666667, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI56549662, Length=480, Percent_Identity=25.4166666666667, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI40254482, Length=480, Percent_Identity=25.4166666666667, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI56549660, Length=480, Percent_Identity=25.4166666666667, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI4502085, Length=480, Percent_Identity=25, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI10280622, Length=480, Percent_Identity=25, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17558728, Length=221, Percent_Identity=29.8642533936652, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17137384, Length=438, Percent_Identity=26.4840182648402, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24654362, Length=439, Percent_Identity=27.5626423690205, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI18859631, Length=439, Percent_Identity=27.5626423690205, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006046
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 50658; Mature: 50527

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLQETQSTGANVILHAFDWKYADIAQRAAEISELGYGSVLVSPPMKSAQDERWWQRYQ
CCCHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHCC
PQDYRVIDNALGNTTDFKNMVEALDRVGVLVYADVVFNHMANEAHLRSDLQYPRQAVMDD
CCCHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
YQQNASKYQDLILFGDLSEPLFDENDFVEAFGIKDWKDKWEVQNGRISGGPTDPGLPTLR
HHHHHHHHHEEEEECCCCCCCCCCCCCHHHCCCCCHHHHHCCCCCEECCCCCCCCCCHHH
VCKHVVEQQRTYLKALKALGVKGFRIDAAKHMTLEHLKLVWTEDITQDVHIFGEIITDGG
HHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ATEEEYELFLEPYLKETRLGAYDFPLFSTIFKAFSKKGSFKSLIDPYCFGQALSNRRAIT
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHCHHHHHHHHCCCCEEE
FAITHDIPNNDVFLDLVMDEENEWLAYAYILGRDGGVPLIYTDLDTSGIKGVDGKPRWQN
EEEEECCCCCCEEEEEEECCCCCEEEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCHHH
AWKDARMAKVVAFHNLVHGEPMTVLEGNDDMLVLQRGEKGILVLNKSARAQSLQLTVNKA
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCEEEEEECCCCCCEEEEEHHHH
LVDMMTGEEVPSGEEIKVAAKSAMLLVEK
HHHHHCCCCCCCCCCEEEEEEEEEEEEEC
>Mature Secondary Structure 
SGLQETQSTGANVILHAFDWKYADIAQRAAEISELGYGSVLVSPPMKSAQDERWWQRYQ
CCHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHCC
PQDYRVIDNALGNTTDFKNMVEALDRVGVLVYADVVFNHMANEAHLRSDLQYPRQAVMDD
CCCHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
YQQNASKYQDLILFGDLSEPLFDENDFVEAFGIKDWKDKWEVQNGRISGGPTDPGLPTLR
HHHHHHHHHEEEEECCCCCCCCCCCCCHHHCCCCCHHHHHCCCCCEECCCCCCCCCCHHH
VCKHVVEQQRTYLKALKALGVKGFRIDAAKHMTLEHLKLVWTEDITQDVHIFGEIITDGG
HHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ATEEEYELFLEPYLKETRLGAYDFPLFSTIFKAFSKKGSFKSLIDPYCFGQALSNRRAIT
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHCHHHHHHHHCCCCEEE
FAITHDIPNNDVFLDLVMDEENEWLAYAYILGRDGGVPLIYTDLDTSGIKGVDGKPRWQN
EEEEECCCCCCEEEEEEECCCCCEEEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCHHH
AWKDARMAKVVAFHNLVHGEPMTVLEGNDDMLVLQRGEKGILVLNKSARAQSLQLTVNKA
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCEEEEEECCCCCCEEEEEHHHH
LVDMMTGEEVPSGEEIKVAAKSAMLLVEK
HHHHHCCCCCCCCCCEEEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2449422 [H]