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The map label for this gene is nadE

Identifier: 156976457

GI number: 156976457

Start: 484425

End: 485255

Strand: Reverse

Name: nadE

Synonym: VIBHAR_05230

Alternate gene names: 156976457

Gene position: 485255-484425 (Counterclockwise)

Preceding gene: 156976460

Following gene: 156976455

Centisome position: 22.02

GC content: 47.53

Gene sequence:

>831_bases
ATGGAACAATCCATCCGCGATGAAATGCGAGTACTCCCTTCTATCGACCCGCATTTCGAAATTGAACGCCGCGTCGCTTT
TATCAAGCGTAAATTACAAGATTCTGGCTGTAAGTCTCTTGTCCTTGGTATCAGTGGAGGCGTTGACTCAACAACGTGTG
GTCGCTTAGCTCAATTGGCTGTAGACCAACTAAACGAAGAATCTGACGACAATGGCTATCAGTTCATCGCCGTTCGCTTG
CCTTACGGTGAGCAAAAGGATGAAGACGAAGCCCAACTTGCTCTTGATTTTATCCAACCAACGCATTCCGTATCGGTAAA
CATCAAAGCAGGTGTGGATGGTCTTCATGCTGCTTCTCACGTTGCATTGGAAGGTACGGGTCTTCTACCAACTGACGCAG
CAAAAGTTGACTTAGTGAAAGGCAATGTAAAAGCACGTGCTCGCATGGTAGCGCAATACGAAATCGCTGGTTATGTTGGT
GGGCTCGTTCTTGGTACTGACCACTCAGCAGAAAACATCACTGGTTTCTACACCAAATTTGGTGATGGCGCGTGCGATAT
GGCACCACTGTTTGGCTTGAGCAAACGTCAAGTTCGCGAAGTTGCTGCAACGCTAGGTGCGCCTGAATTGCTTGTGAAAA
AAGTGCCAACGGCAGATTTAGAAGAGCTTGCTCCACAAAAAGCAGACGAAGATGCGCTAAGCCTGACTTACGAGCAAATC
GATGACTTCCTAGAAGGTAAGCCAGTATCGCAAGAAGTGAGCGATCGTTTGGTAGCTATCTACAAAATGACTCAGCACAA
GCGTCAGCCGATCCCAACGATCTACGACTAA

Upstream 100 bases:

>100_bases
GGCGGTTTCTCGCCACCTGGTGAACGATATACGTCTTAAGCAGTATGAATCTAGTAATACTGATTAGTGTCGAACCTATT
TGCAGGAAAAGGAACCCTAA

Downstream 100 bases:

>100_bases
AACGCGATTGTGCGCATGAGTGTTCCCTCTCTGAGCCCGTTCTAAGATAGAAAAAACGCCGCTAAGTTAGCGGCGTTTTT
ATTTGCTGGTCATGAGCAGC

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MEQSIRDEMRVLPSIDPHFEIERRVAFIKRKLQDSGCKSLVLGISGGVDSTTCGRLAQLAVDQLNEESDDNGYQFIAVRL
PYGEQKDEDEAQLALDFIQPTHSVSVNIKAGVDGLHAASHVALEGTGLLPTDAAKVDLVKGNVKARARMVAQYEIAGYVG
GLVLGTDHSAENITGFYTKFGDGACDMAPLFGLSKRQVREVAATLGAPELLVKKVPTADLEELAPQKADEDALSLTYEQI
DDFLEGKPVSQEVSDRLVAIYKMTQHKRQPIPTIYD

Sequences:

>Translated_276_residues
MEQSIRDEMRVLPSIDPHFEIERRVAFIKRKLQDSGCKSLVLGISGGVDSTTCGRLAQLAVDQLNEESDDNGYQFIAVRL
PYGEQKDEDEAQLALDFIQPTHSVSVNIKAGVDGLHAASHVALEGTGLLPTDAAKVDLVKGNVKARARMVAQYEIAGYVG
GLVLGTDHSAENITGFYTKFGDGACDMAPLFGLSKRQVREVAATLGAPELLVKKVPTADLEELAPQKADEDALSLTYEQI
DDFLEGKPVSQEVSDRLVAIYKMTQHKRQPIPTIYD
>Mature_276_residues
MEQSIRDEMRVLPSIDPHFEIERRVAFIKRKLQDSGCKSLVLGISGGVDSTTCGRLAQLAVDQLNEESDDNGYQFIAVRL
PYGEQKDEDEAQLALDFIQPTHSVSVNIKAGVDGLHAASHVALEGTGLLPTDAAKVDLVKGNVKARARMVAQYEIAGYVG
GLVLGTDHSAENITGFYTKFGDGACDMAPLFGLSKRQVREVAATLGAPELLVKKVPTADLEELAPQKADEDALSLTYEQI
DDFLEGKPVSQEVSDRLVAIYKMTQHKRQPIPTIYD

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family

Homologues:

Organism=Escherichia coli, GI1788036, Length=277, Percent_Identity=49.4584837545126, Blast_Score=247, Evalue=5e-67,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NADE_VIBHB (A7N654)

Other databases:

- EMBL:   CP000790
- RefSeq:   YP_001447363.1
- ProteinModelPortal:   A7N654
- SMR:   A7N654
- STRING:   A7N654
- GeneID:   5557193
- GenomeReviews:   CP000790_GR
- KEGG:   vha:VIBHAR_05230
- NMPDR:   fig|338187.4.peg.3058
- eggNOG:   COG0171
- HOGENOM:   HBG351567
- OMA:   KVAQATI
- ProtClustDB:   PRK00768
- HAMAP:   MF_00193
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase

EC number: =6.3.1.5

Molecular weight: Translated: 30085; Mature: 30085

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: ACT_SITE 45-45

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQSIRDEMRVLPSIDPHFEIERRVAFIKRKLQDSGCKSLVLGISGGVDSTTCGRLAQLA
CCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHH
VDQLNEESDDNGYQFIAVRLPYGEQKDEDEAQLALDFIQPTHSVSVNIKAGVDGLHAASH
HHHHCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHE
VALEGTGLLPTDAAKVDLVKGNVKARARMVAQYEIAGYVGGLVLGTDHSAENITGFYTKF
EEEECCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCEEHHHCC
GDGACDMAPLFGLSKRQVREVAATLGAPELLVKKVPTADLEELAPQKADEDALSLTYEQI
CCCCCCHHHHCCCCHHHHHHHHHHCCCHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHH
DDFLEGKPVSQEVSDRLVAIYKMTQHKRQPIPTIYD
HHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MEQSIRDEMRVLPSIDPHFEIERRVAFIKRKLQDSGCKSLVLGISGGVDSTTCGRLAQLA
CCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHH
VDQLNEESDDNGYQFIAVRLPYGEQKDEDEAQLALDFIQPTHSVSVNIKAGVDGLHAASH
HHHHCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHE
VALEGTGLLPTDAAKVDLVKGNVKARARMVAQYEIAGYVGGLVLGTDHSAENITGFYTKF
EEEECCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCEEHHHCC
GDGACDMAPLFGLSKRQVREVAATLGAPELLVKKVPTADLEELAPQKADEDALSLTYEQI
CCCCCCHHHHCCCCHHHHHHHHHHCCCHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHH
DDFLEGKPVSQEVSDRLVAIYKMTQHKRQPIPTIYD
HHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA