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The map label for this gene is pleD [H]

Identifier: 156976342

GI number: 156976342

Start: 351609

End: 353192

Strand: Reverse

Name: pleD [H]

Synonym: VIBHAR_05115

Alternate gene names: 156976342

Gene position: 353192-351609 (Counterclockwise)

Preceding gene: 156976343

Following gene: 156976339

Centisome position: 16.02

GC content: 46.78

Gene sequence:

>1584_bases
ATGGAACAACTGCTTAGTAAGGTGACTGATGCTGGCATGGATCCCTCTTCCGTTGCCGGTGAAGAAGCGCTAATCCTATG
GGAACACATCCGTCTGCATATCGCCTCGACCAATCAAGAGAAGGCGCACTGCTATATCATCAGCGCGGAATATCGAAGTA
AGATTAAACAGCTACAAGAGAGCATTGAAGAGCTGCGTCATGCACTCGATTTACTTAAACTGCCAGAAGACGTTGAGGAT
ATCCTCTCTGTCAAATCCAGCTTGAGTGACCGATTGGTAGAAAAAGGCGATTATAACTCTGCACTCACCGAGTACATTGC
TTCCACCAACATTGCCGTCGAATACGGTTATATCGATGAGTATGTTCTGGCAATCATCGGCATGGGCAACCTTTGTGACG
CGTATGGCGACCACAACCGCGCTCTTCGTTACTACCAAAAAATCGATGCGATTGACCATGCCGTCGGCAGCCGTTCATTG
AGGTTACGCTATAAGCTGTACATGCTAGCTTGTTATATCAGTTTGCAACGTATAGGCGCGGCGACGGACCTCTTAAGCGA
GTGCGAAGAGCTCAGTATCCTCGTCAGCGATAAAAATCTGACAGGACAAATTCACTTATACCGAGCTAAGTTGCTTCGAC
TGCAAAAAGAGTACAACAAAGCACTACTTGCTATCTCTCAGATTCAATACGCAACAGGCAAGCAACATACTGTTTGGCTC
TCGACCATGGTAAAGATCGAGACGGCTTACTGTTTGAATGAAGTTGGTCATCAATCATTATCAAACCTATTGTTAGCTTC
TACGGGGAAGCGCATCAACGAGCAAGCATCGCCTTTGATCGCGAATCGTTTTTATAACGCGATGAGCCAAGTGTGTGCTT
CTCAAGGCTTGTTCAAAGAAGCGCTCAACTACGAGAAAAAAGCCTTCCGAGTCGAATCTGATTTAGTGAAACATATTCCG
ATCAGTGAACTTGGCCCAGCTCAGCTGCGTCGCCTGAGTCGATTTGAATTACAGCTCAAGTTGATCCTTTCAGAGATTGA
GAACAAAGAGCTCAAAGAAACCACTAAACAACACAAAAACACCGTTGCTCAGCTTCAACAAGATGTCTTTACCGACCCGT
TAACGTCACTCCACAACCGTCGTTGGCTAGAAGTGAAGCTGAAAGACTTGTTGCTGCAAGATACGCCATTTGCCCTAATG
GTGATCGACATTGACCACTTTAAATCAATCAATGATGAGCTTAGCCACTTAGTGGGCGACAAAGCCATTGTGAACGTCTC
CAAGGAGCTTTCTGGGTACTTCAAGTTCCGTGGTTCATCGTGTGTACGTTTCGGTGGTGAAGAGTTCTTGGTGATTCTAG
AAAACACCGAGGTTGCCAAGGCAGAAATGCACGCCGAAAACTACCGCGAGCGCATTTACCAGTTTGGTTGGCATGAAATC
TTAGGCGAGCGTGGCTTGACCGTCTCCATCGGCATTACCTTACATCGGGAGGGTGAGAATACTCAGCGCACTTTCTACCG
TGCCGACAAAGCTCTATACCGCGCGAAAGCCAATGGTCGAAACCAAGTGTGTACTGAGCTATAA

Upstream 100 bases:

>100_bases
GGTTTAACGGGCCCTAAGCCAACACTTTATCTACAGGACATCCTAACCTTCCCGTTCACCTTAGAGCAGTTGGAAGAGAG
CCACATTGAGGTAATGAAGA

Downstream 100 bases:

>100_bases
GCCAGATTTTCAATCTATAAAAAACGCCCCAGCTCAAACTCTGAGGGATCCCCACATCTTTTCACCGGAACTCTGCCGCA
CTTATCGCTTTGAGGCCTCC

Product: hypothetical protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MEQLLSKVTDAGMDPSSVAGEEALILWEHIRLHIASTNQEKAHCYIISAEYRSKIKQLQESIEELRHALDLLKLPEDVED
ILSVKSSLSDRLVEKGDYNSALTEYIASTNIAVEYGYIDEYVLAIIGMGNLCDAYGDHNRALRYYQKIDAIDHAVGSRSL
RLRYKLYMLACYISLQRIGAATDLLSECEELSILVSDKNLTGQIHLYRAKLLRLQKEYNKALLAISQIQYATGKQHTVWL
STMVKIETAYCLNEVGHQSLSNLLLASTGKRINEQASPLIANRFYNAMSQVCASQGLFKEALNYEKKAFRVESDLVKHIP
ISELGPAQLRRLSRFELQLKLILSEIENKELKETTKQHKNTVAQLQQDVFTDPLTSLHNRRWLEVKLKDLLLQDTPFALM
VIDIDHFKSINDELSHLVGDKAIVNVSKELSGYFKFRGSSCVRFGGEEFLVILENTEVAKAEMHAENYRERIYQFGWHEI
LGERGLTVSIGITLHREGENTQRTFYRADKALYRAKANGRNQVCTEL

Sequences:

>Translated_527_residues
MEQLLSKVTDAGMDPSSVAGEEALILWEHIRLHIASTNQEKAHCYIISAEYRSKIKQLQESIEELRHALDLLKLPEDVED
ILSVKSSLSDRLVEKGDYNSALTEYIASTNIAVEYGYIDEYVLAIIGMGNLCDAYGDHNRALRYYQKIDAIDHAVGSRSL
RLRYKLYMLACYISLQRIGAATDLLSECEELSILVSDKNLTGQIHLYRAKLLRLQKEYNKALLAISQIQYATGKQHTVWL
STMVKIETAYCLNEVGHQSLSNLLLASTGKRINEQASPLIANRFYNAMSQVCASQGLFKEALNYEKKAFRVESDLVKHIP
ISELGPAQLRRLSRFELQLKLILSEIENKELKETTKQHKNTVAQLQQDVFTDPLTSLHNRRWLEVKLKDLLLQDTPFALM
VIDIDHFKSINDELSHLVGDKAIVNVSKELSGYFKFRGSSCVRFGGEEFLVILENTEVAKAEMHAENYRERIYQFGWHEI
LGERGLTVSIGITLHREGENTQRTFYRADKALYRAKANGRNQVCTEL
>Mature_527_residues
MEQLLSKVTDAGMDPSSVAGEEALILWEHIRLHIASTNQEKAHCYIISAEYRSKIKQLQESIEELRHALDLLKLPEDVED
ILSVKSSLSDRLVEKGDYNSALTEYIASTNIAVEYGYIDEYVLAIIGMGNLCDAYGDHNRALRYYQKIDAIDHAVGSRSL
RLRYKLYMLACYISLQRIGAATDLLSECEELSILVSDKNLTGQIHLYRAKLLRLQKEYNKALLAISQIQYATGKQHTVWL
STMVKIETAYCLNEVGHQSLSNLLLASTGKRINEQASPLIANRFYNAMSQVCASQGLFKEALNYEKKAFRVESDLVKHIP
ISELGPAQLRRLSRFELQLKLILSEIENKELKETTKQHKNTVAQLQQDVFTDPLTSLHNRRWLEVKLKDLLLQDTPFALM
VIDIDHFKSINDELSHLVGDKAIVNVSKELSGYFKFRGSSCVRFGGEEFLVILENTEVAKAEMHAENYRERIYQFGWHEI
LGERGLTVSIGITLHREGENTQRTFYRADKALYRAKANGRNQVCTEL

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=157, Percent_Identity=36.3057324840764, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI87081881, Length=166, Percent_Identity=35.5421686746988, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI145693134, Length=198, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786584, Length=161, Percent_Identity=36.0248447204969, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787802, Length=162, Percent_Identity=32.7160493827161, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI87082007, Length=171, Percent_Identity=32.1637426900585, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1787056, Length=160, Percent_Identity=31.25, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1787541, Length=164, Percent_Identity=30.4878048780488, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788956, Length=187, Percent_Identity=28.8770053475936, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787816, Length=160, Percent_Identity=31.875, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1788085, Length=159, Percent_Identity=32.0754716981132, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 60069; Mature: 60069

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQLLSKVTDAGMDPSSVAGEEALILWEHIRLHIASTNQEKAHCYIISAEYRSKIKQLQE
CHHHHHHHHHCCCCCCCCCCCHHEEEEEEHEEEEECCCCCCCEEEEEEHHHHHHHHHHHH
SIEELRHALDLLKLPEDVEDILSVKSSLSDRLVEKGDYNSALTEYIASTNIAVEYGYIDE
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEECCHHH
YVLAIIGMGNLCDAYGDHNRALRYYQKIDAIDHAVGSRSLRLRYKLYMLACYISLQRIGA
HHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
ATDLLSECEELSILVSDKNLTGQIHLYRAKLLRLQKEYNKALLAISQIQYATGKQHTVWL
HHHHHHHHHHCEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEH
STMVKIETAYCLNEVGHQSLSNLLLASTGKRINEQASPLIANRFYNAMSQVCASQGLFKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHCCHHHH
ALNYEKKAFRVESDLVKHIPISELGPAQLRRLSRFELQLKLILSEIENKELKETTKQHKN
HHCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
TVAQLQQDVFTDPLTSLHNRRWLEVKLKDLLLQDTPFALMVIDIDHFKSINDELSHLVGD
HHHHHHHHHHCCHHHHHCCCEEEEEEHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHCC
KAIVNVSKELSGYFKFRGSSCVRFGGEEFLVILENTEVAKAEMHAENYRERIYQFGWHEI
HHHHHHHHHHHHHHEECCCCEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
LGERGLTVSIGITLHREGENTQRTFYRADKALYRAKANGRNQVCTEL
HCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCHHCCC
>Mature Secondary Structure
MEQLLSKVTDAGMDPSSVAGEEALILWEHIRLHIASTNQEKAHCYIISAEYRSKIKQLQE
CHHHHHHHHHCCCCCCCCCCCHHEEEEEEHEEEEECCCCCCCEEEEEEHHHHHHHHHHHH
SIEELRHALDLLKLPEDVEDILSVKSSLSDRLVEKGDYNSALTEYIASTNIAVEYGYIDE
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEECCHHH
YVLAIIGMGNLCDAYGDHNRALRYYQKIDAIDHAVGSRSLRLRYKLYMLACYISLQRIGA
HHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
ATDLLSECEELSILVSDKNLTGQIHLYRAKLLRLQKEYNKALLAISQIQYATGKQHTVWL
HHHHHHHHHHCEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEH
STMVKIETAYCLNEVGHQSLSNLLLASTGKRINEQASPLIANRFYNAMSQVCASQGLFKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHCCHHHH
ALNYEKKAFRVESDLVKHIPISELGPAQLRRLSRFELQLKLILSEIENKELKETTKQHKN
HHCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
TVAQLQQDVFTDPLTSLHNRRWLEVKLKDLLLQDTPFALMVIDIDHFKSINDELSHLVGD
HHHHHHHHHHCCHHHHHCCCEEEEEEHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHCC
KAIVNVSKELSGYFKFRGSSCVRFGGEEFLVILENTEVAKAEMHAENYRERIYQFGWHEI
HHHHHHHHHHHHHHEECCCCEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
LGERGLTVSIGITLHREGENTQRTFYRADKALYRAKANGRNQVCTEL
HCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA