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The map label for this gene is lafS [H]

Identifier: 156976180

GI number: 156976180

Start: 210220

End: 210948

Strand: Reverse

Name: lafS [H]

Synonym: VIBHAR_04951

Alternate gene names: 156976180

Gene position: 210948-210220 (Counterclockwise)

Preceding gene: 156976181

Following gene: 156976179

Centisome position: 9.57

GC content: 51.58

Gene sequence:

>729_bases
ATGTTGGAGATGAACTTACAGGAAAGTTATACCGCGACGGAGGAAGTAAATACGCCGTCGCGCCCGATTGATGAAAATGC
ACTGCTGCAACGTCACCAAGTCATGGTTAAGCGCGTAGTAAATCAACTGCGCGTTCATGCGACCTCGCACTGCAGCATTG
AAGACATGCAGCAAATCGGCTTGATTGCGCTTGTCGAAGCTGGGCGTCGTTATGGGGATGTGGATGATCCACACTTCCCC
GCTTTTGCCGTTTGTCGTGTTCGTGGCGCGATTTTGGACGAGCTTCGTCGTTTGGATTGGCGCTCACGCAAAACCCGCCA
ACAGGCGCATGAGCTTAACGATGTGACACGAGATCTTACTCGGGCGCTGGGCAGAATGCCCACCGATGCTGAAATCATCA
AAGCGCTAGGTACGGATGAGCAAGACTACTATAACCGCCAAAACGCCGCACTCGCTGGCGAAATGCAAAGCCTCGACCAA
TTGATGGAAACTGGCGGAGAGAGCCACTTTGGCGGCCAATATGACGGCATGGAACATGAGCACATTCGCCGTAGCTTAGA
CACTGCGCTGGGCAAAATGTCGAAACGTGACCAACTGTTGCTCACCTTGTTCTATCAACATGAACTCAACCTCCATGAAA
TCGCGCTCGTGCTCGATCTGACACCACCTCGCATCTGTCAGTTGCACAAACAAGCGCTTAAACAACTCAACCAATTAATG
TCCTCTTAG

Upstream 100 bases:

>100_bases
CAAAGAGACTCTGCTGCTTGCGTTCGCAAAAACCGACATCGTTCGTGATATCGATGATGTTCTGCTAACCAAGTACGTTG
TGCAATAACGGAGCACAGCC

Downstream 100 bases:

>100_bases
GAGTCACAAGATGCAAAAGTTTTTTGGAGCCATTACCGTACTGATGTGTGTCTTCGGCGGATACATGTGGGCAGGCGGTA
AACTCGGCGCTATCTGGCAG

Product: DNA-directed RNA polymerase sigma subunit/flagellum specific

Products: NA

Alternate protein names: Sigma-27; Sigma-F factor [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MLEMNLQESYTATEEVNTPSRPIDENALLQRHQVMVKRVVNQLRVHATSHCSIEDMQQIGLIALVEAGRRYGDVDDPHFP
AFAVCRVRGAILDELRRLDWRSRKTRQQAHELNDVTRDLTRALGRMPTDAEIIKALGTDEQDYYNRQNAALAGEMQSLDQ
LMETGGESHFGGQYDGMEHEHIRRSLDTALGKMSKRDQLLLTLFYQHELNLHEIALVLDLTPPRICQLHKQALKQLNQLM
SS

Sequences:

>Translated_242_residues
MLEMNLQESYTATEEVNTPSRPIDENALLQRHQVMVKRVVNQLRVHATSHCSIEDMQQIGLIALVEAGRRYGDVDDPHFP
AFAVCRVRGAILDELRRLDWRSRKTRQQAHELNDVTRDLTRALGRMPTDAEIIKALGTDEQDYYNRQNAALAGEMQSLDQ
LMETGGESHFGGQYDGMEHEHIRRSLDTALGKMSKRDQLLLTLFYQHELNLHEIALVLDLTPPRICQLHKQALKQLNQLM
SS
>Mature_242_residues
MLEMNLQESYTATEEVNTPSRPIDENALLQRHQVMVKRVVNQLRVHATSHCSIEDMQQIGLIALVEAGRRYGDVDDPHFP
AFAVCRVRGAILDELRRLDWRSRKTRQQAHELNDVTRDLTRALGRMPTDAEIIKALGTDEQDYYNRQNAALAGEMQSLDQ
LMETGGESHFGGQYDGMEHEHIRRSLDTALGKMSKRDQLLLTLFYQHELNLHEIALVLDLTPPRICQLHKQALKQLNQLM
SS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=227, Percent_Identity=33.920704845815, Blast_Score=119, Evalue=2e-28,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27737; Mature: 27737

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEMNLQESYTATEEVNTPSRPIDENALLQRHQVMVKRVVNQLRVHATSHCSIEDMQQIG
CCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LIALVEAGRRYGDVDDPHFPAFAVCRVRGAILDELRRLDWRSRKTRQQAHELNDVTRDLT
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RALGRMPTDAEIIKALGTDEQDYYNRQNAALAGEMQSLDQLMETGGESHFGGQYDGMEHE
HHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
HIRRSLDTALGKMSKRDQLLLTLFYQHELNLHEIALVLDLTPPRICQLHKQALKQLNQLM
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHEEEEEECCCHHHHHHHHHHHHHHHHHH
SS
CC
>Mature Secondary Structure
MLEMNLQESYTATEEVNTPSRPIDENALLQRHQVMVKRVVNQLRVHATSHCSIEDMQQIG
CCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LIALVEAGRRYGDVDDPHFPAFAVCRVRGAILDELRRLDWRSRKTRQQAHELNDVTRDLT
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RALGRMPTDAEIIKALGTDEQDYYNRQNAALAGEMQSLDQLMETGGESHFGGQYDGMEHE
HHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
HIRRSLDTALGKMSKRDQLLLTLFYQHELNLHEIALVLDLTPPRICQLHKQALKQLNQLM
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHEEEEEECCCHHHHHHHHHHHHHHHHHH
SS
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501040; 12620739 [H]