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The map label for this gene is mltD [H]

Identifier: 156976134

GI number: 156976134

Start: 172642

End: 173319

Strand: Reverse

Name: mltD [H]

Synonym: VIBHAR_04905

Alternate gene names: 156976134

Gene position: 173319-172642 (Counterclockwise)

Preceding gene: 156976135

Following gene: 156976133

Centisome position: 7.86

GC content: 46.9

Gene sequence:

>678_bases
ATGACTGTAGGCTTTTTTGTTTTTTGCTTGACCTTCTTGTCTCCATTGACTTTGGCCAAGCCGCACAACAAAACCATAGA
GCAACAATTCCAAGTGCTCGCTCCTTACCAAACACAAATTAACCAACGTCTCGATTCCGTTAGCCCGCTACTCGATCATA
TCTTTAAGCAGCTAAAGCATAAATCTCTGCCTGCAAACTTGGTGCTGGTGCCGATGCTGGAATCTTCCTACAACCCCAAA
GCCGTATCGCACGCCAATGCTGCGGGCCTCTGGCAGTTGATCCCTGCCACGGCAACACGCTTTGGTTTGCAAGTAACGAA
TAGTCAGGATGAGCGTTTTGATACCAAAGCCAGCACGAAAGCCGCATTGGAGTATTTGGCGTTTTTGTATAACAAATTTG
ATCAAGATATCGCGCTAACCCTTGCAGCTTATAACGCAGGTGAAGGCCGTGTCGCTAGAGCGATAAAGAAGGCTGGCAGT
CAAGATTTCAAAGCACTCACATTGCCGAAAGAGACTCAGCAATACGTCAATCGCTTTTACGCGCTGGAACGTTTGGTCAA
TATTCAGAAACTGCGCACCGATTCTTTCCAACCACTACTTTTGTTTGCGAATCATTCCTCGACCTACACCGAACCGCTTA
TAGACCTTAGTCGATTACCGCCACTCGTGGAATTGTGA

Upstream 100 bases:

>100_bases
GTCAGTCAACTCAAACAACCTGTGCAACACACTTATGAAGAGCGTTCGTTCTAAACAACGATTCTCGCTTTGCCTACAGA
GTCTTCCGCCATCCCGGTCG

Downstream 100 bases:

>100_bases
ACATTCTCACTAAAAGCTACCAATGTCAGAAGCACGTAAAAACACAAGATCCTATTCCGCCAATTTACAAACTCGTGATC
TCGATGCAAACGCGTGCATA

Product: membrane-bound lytic murein transglycosylase D

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MTVGFFVFCLTFLSPLTLAKPHNKTIEQQFQVLAPYQTQINQRLDSVSPLLDHIFKQLKHKSLPANLVLVPMLESSYNPK
AVSHANAAGLWQLIPATATRFGLQVTNSQDERFDTKASTKAALEYLAFLYNKFDQDIALTLAAYNAGEGRVARAIKKAGS
QDFKALTLPKETQQYVNRFYALERLVNIQKLRTDSFQPLLLFANHSSTYTEPLIDLSRLPPLVEL

Sequences:

>Translated_225_residues
MTVGFFVFCLTFLSPLTLAKPHNKTIEQQFQVLAPYQTQINQRLDSVSPLLDHIFKQLKHKSLPANLVLVPMLESSYNPK
AVSHANAAGLWQLIPATATRFGLQVTNSQDERFDTKASTKAALEYLAFLYNKFDQDIALTLAAYNAGEGRVARAIKKAGS
QDFKALTLPKETQQYVNRFYALERLVNIQKLRTDSFQPLLLFANHSSTYTEPLIDLSRLPPLVEL
>Mature_224_residues
TVGFFVFCLTFLSPLTLAKPHNKTIEQQFQVLAPYQTQINQRLDSVSPLLDHIFKQLKHKSLPANLVLVPMLESSYNPKA
VSHANAAGLWQLIPATATRFGLQVTNSQDERFDTKASTKAALEYLAFLYNKFDQDIALTLAAYNAGEGRVARAIKKAGSQ
DFKALTLPKETQQYVNRFYALERLVNIQKLRTDSFQPLLLFANHSSTYTEPLIDLSRLPPLVEL

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=179, Percent_Identity=41.340782122905, Blast_Score=136, Evalue=1e-33,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 25343; Mature: 25212

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVGFFVFCLTFLSPLTLAKPHNKTIEQQFQVLAPYQTQINQRLDSVSPLLDHIFKQLKH
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
KSLPANLVLVPMLESSYNPKAVSHANAAGLWQLIPATATRFGLQVTNSQDERFDTKASTK
CCCCCCEEEEEECCCCCCCCHHCCCCHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHH
AALEYLAFLYNKFDQDIALTLAAYNAGEGRVARAIKKAGSQDFKALTLPKETQQYVNRFY
HHHHHHHHHHHHHCCHHEEEEEECCCCCHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHH
ALERLVNIQKLRTDSFQPLLLFANHSSTYTEPLIDLSRLPPLVEL
HHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TVGFFVFCLTFLSPLTLAKPHNKTIEQQFQVLAPYQTQINQRLDSVSPLLDHIFKQLKH
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
KSLPANLVLVPMLESSYNPKAVSHANAAGLWQLIPATATRFGLQVTNSQDERFDTKASTK
CCCCCCEEEEEECCCCCCCCHHCCCCHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHH
AALEYLAFLYNKFDQDIALTLAAYNAGEGRVARAIKKAGSQDFKALTLPKETQQYVNRFY
HHHHHHHHHHHHHCCHHEEEEEECCCCCHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHH
ALERLVNIQKLRTDSFQPLLLFANHSSTYTEPLIDLSRLPPLVEL
HHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]