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The map label for this gene is ahpF [H]

Identifier: 156975991

GI number: 156975991

Start: 27945

End: 29513

Strand: Reverse

Name: ahpF [H]

Synonym: VIBHAR_04761

Alternate gene names: 156975991

Gene position: 29513-27945 (Counterclockwise)

Preceding gene: 156975992

Following gene: 156975988

Centisome position: 1.34

GC content: 47.55

Gene sequence:

>1569_bases
ATGTTAGACCAAGCGATCCAACAACAACTCAAACAATATCTGGCTAATGTAAAAGAAGAAGTTCGTTTAGTGGTTAGTCT
AGATGAAAGCAAAGCATCGAATGACATTTTGTCTCTAGCGAACCAAATCGCAGAGATGAGCGACCTAATCACCGTTGAAC
GTGACGAAAACGCAAGCGCACGTAAACCAGTGATGGCGGTAACTAACCCAGCAAAAGGCACGGCACTACGCTTTGCAGGC
TTGCCAATGGGTCACGAATTTACCTCTCTAGTATTGGCTCTGCTTCATTCTGGCGGTCACCCAATCAAACTAGAACCAGA
AGTGATTGAGCAAATCGCAGCACTAGACAAAGATCTAGTCGTAGAAATCTTCATTTCACTGTCGTGTCAAAACTGTCCGG
ACGTGGTTCAAGCGTTCAACATGATGGCGGCGATCAACCCGAAAGTAAAAGCAACCATGATCGACGGTGGTTTGTTCCAA
GATGAAGTGAAACAACGCGACATCATGGCTGTACCAAGTGTATTCGTGAACGGTGAGCTATTCGGTCAAGGTCGTATGTC
TCTGACTGAAATCCTAAACAAAGTGGATGACGGCGCGAGCGAGAAAGCGGCGAAAGCACTGAGCGAAAAAGACCCATACG
ACGTACTTGTTGTCGGCGGTGGCCCTGGTGGTAGCGCAGCTGCTCTATACGCTGCTCGTAAAGGAATTCGTACTGGCGTT
GTTGCTAAACGCTTTGGTGGTCAGGTTATGGACACCATGGCAATTGAGAACTTCATTTCGGTAAAACGTACTGAAGGTCC
AAAACTGGCGACTGACCTAGCAGAGCACCTAAAAGAATACGATGTAGACGTAGTAACTGAGCAACAAGCTGAGAAGCTAA
TGGGCGCAGCACACACAGAAGATGGTTTGATTCACATCCAGCTTGAAAGCGGCGCAACACTGAAGAGTAAGAGTGTCATC
CTTAGCCCTGGTGCACGCTGGCGTGAAATGAACGTTCCGGGTGAGCAAGAGTACCGTAACAAAGGTGTCGCGTACTGCCC
ACACTGTGACGGTCCACTGTTCAAAGGCAAGAAAACTGCGGTTATCGGTGGTGGTAACTCAGGCATCGAAGCAGCTATCG
ACCTTGCTGGTATCGTAGAACACGTAACGGTTCTTGAATTCGCAGACGTACTTCGCGCAGACCAAGTGCTTATCGACAAA
GCGAACTCACTACCAAACGTAGACATCATCACGATGGCGCAAACAACAGAAGTAGTAGGCGACGGTACTCGCGTAACAAG
CTTGAAGTACAAAGACCGCAACACAGATGAAATCAAAGAGATTGAACTGTCAGGTATCTTTGTTCAAATCGGTCTAGTAC
CAAACACAGAATGGTTAAAAGATTCAGAAGTAGCCCTATCTGAGCGCGGCGAAATTGAAGTCGGTAGCCGTGGCGATACT
AGCCTAGAAGGTGTGTTCGCAGCTGGTGACGCAACAACCATTCCTTACAAACAGATCATCATTGCGATGGGTGAAGGTGC
AAAAGCAAGCTTAGGCGCATTTGATTACCTAATCCGTAAGCAAGGTTAA

Upstream 100 bases:

>100_bases
AGGTTAGATACCTTGACGACCTTCTGCCTGATTTGCTCTCTCCCCCTCAGGTTTTATCTACAGTAACCACTTTTTAGTTT
TAATTGAGGTAGTAACAACT

Downstream 100 bases:

>100_bases
TCTCCAATTTCTTGAGCACAAAGCGCACGGTGCTGTGTTTTCTTCCCCGGAAAACCAATCGGGGCACCGTGACAGCGATG
AGTAACAAGAAATAAAATCA

Product: alkyl hydroperoxide reductase, subunit F

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MLDQAIQQQLKQYLANVKEEVRLVVSLDESKASNDILSLANQIAEMSDLITVERDENASARKPVMAVTNPAKGTALRFAG
LPMGHEFTSLVLALLHSGGHPIKLEPEVIEQIAALDKDLVVEIFISLSCQNCPDVVQAFNMMAAINPKVKATMIDGGLFQ
DEVKQRDIMAVPSVFVNGELFGQGRMSLTEILNKVDDGASEKAAKALSEKDPYDVLVVGGGPGGSAAALYAARKGIRTGV
VAKRFGGQVMDTMAIENFISVKRTEGPKLATDLAEHLKEYDVDVVTEQQAEKLMGAAHTEDGLIHIQLESGATLKSKSVI
LSPGARWREMNVPGEQEYRNKGVAYCPHCDGPLFKGKKTAVIGGGNSGIEAAIDLAGIVEHVTVLEFADVLRADQVLIDK
ANSLPNVDIITMAQTTEVVGDGTRVTSLKYKDRNTDEIKEIELSGIFVQIGLVPNTEWLKDSEVALSERGEIEVGSRGDT
SLEGVFAAGDATTIPYKQIIIAMGEGAKASLGAFDYLIRKQG

Sequences:

>Translated_522_residues
MLDQAIQQQLKQYLANVKEEVRLVVSLDESKASNDILSLANQIAEMSDLITVERDENASARKPVMAVTNPAKGTALRFAG
LPMGHEFTSLVLALLHSGGHPIKLEPEVIEQIAALDKDLVVEIFISLSCQNCPDVVQAFNMMAAINPKVKATMIDGGLFQ
DEVKQRDIMAVPSVFVNGELFGQGRMSLTEILNKVDDGASEKAAKALSEKDPYDVLVVGGGPGGSAAALYAARKGIRTGV
VAKRFGGQVMDTMAIENFISVKRTEGPKLATDLAEHLKEYDVDVVTEQQAEKLMGAAHTEDGLIHIQLESGATLKSKSVI
LSPGARWREMNVPGEQEYRNKGVAYCPHCDGPLFKGKKTAVIGGGNSGIEAAIDLAGIVEHVTVLEFADVLRADQVLIDK
ANSLPNVDIITMAQTTEVVGDGTRVTSLKYKDRNTDEIKEIELSGIFVQIGLVPNTEWLKDSEVALSERGEIEVGSRGDT
SLEGVFAAGDATTIPYKQIIIAMGEGAKASLGAFDYLIRKQG
>Mature_522_residues
MLDQAIQQQLKQYLANVKEEVRLVVSLDESKASNDILSLANQIAEMSDLITVERDENASARKPVMAVTNPAKGTALRFAG
LPMGHEFTSLVLALLHSGGHPIKLEPEVIEQIAALDKDLVVEIFISLSCQNCPDVVQAFNMMAAINPKVKATMIDGGLFQ
DEVKQRDIMAVPSVFVNGELFGQGRMSLTEILNKVDDGASEKAAKALSEKDPYDVLVVGGGPGGSAAALYAARKGIRTGV
VAKRFGGQVMDTMAIENFISVKRTEGPKLATDLAEHLKEYDVDVVTEQQAEKLMGAAHTEDGLIHIQLESGATLKSKSVI
LSPGARWREMNVPGEQEYRNKGVAYCPHCDGPLFKGKKTAVIGGGNSGIEAAIDLAGIVEHVTVLEFADVLRADQVLIDK
ANSLPNVDIITMAQTTEVVGDGTRVTSLKYKDRNTDEIKEIELSGIFVQIGLVPNTEWLKDSEVALSERGEIEVGSRGDT
SLEGVFAAGDATTIPYKQIIIAMGEGAKASLGAFDYLIRKQG

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=521, Percent_Identity=59.6928982725528, Blast_Score=627, Evalue=0.0,
Organism=Escherichia coli, GI1787114, Length=314, Percent_Identity=31.8471337579618, Blast_Score=149, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6320560, Length=313, Percent_Identity=31.9488817891374, Blast_Score=134, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6321898, Length=321, Percent_Identity=30.2180685358255, Blast_Score=131, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 56060; Mature: 56060

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDQAIQQQLKQYLANVKEEVRLVVSLDESKASNDILSLANQIAEMSDLITVERDENASA
CCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCC
RKPVMAVTNPAKGTALRFAGLPMGHEFTSLVLALLHSGGHPIKLEPEVIEQIAALDKDLV
CCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEECHHHHHHHHHCCHHHH
VEIFISLSCQNCPDVVQAFNMMAAINPKVKATMIDGGLFQDEVKQRDIMAVPSVFVNGEL
HHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCEECCCEEECCEE
FGQGRMSLTEILNKVDDGASEKAAKALSEKDPYDVLVVGGGPGGSAAALYAARKGIRTGV
ECCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHCCHHHH
VAKRFGGQVMDTMAIENFISVKRTEGPKLATDLAEHLKEYDVDVVTEQQAEKLMGAAHTE
HHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEECHHHHHHHHCCCCCC
DGLIHIQLESGATLKSKSVILSPGARWREMNVPGEQEYRNKGVAYCPHCDGPLFKGKKTA
CCEEEEEECCCCCEECCEEEECCCCCEEECCCCCHHHHHCCCCEECCCCCCCCCCCCEEE
VIGGGNSGIEAAIDLAGIVEHVTVLEFADVLRADQVLIDKANSLPNVDIITMAQTTEVVG
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEHHHHHC
DGTRVTSLKYKDRNTDEIKEIELSGIFVQIGLVPNTEWLKDSEVALSERGEIEVGSRGDT
CCCEEEEEEECCCCCCHHHEEEEEEEEEEEEECCCCCCCCCCCHHHCCCCCEEECCCCCC
SLEGVFAAGDATTIPYKQIIIAMGEGAKASLGAFDYLIRKQG
CCCEEEECCCCCCCCHHHEEEEECCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MLDQAIQQQLKQYLANVKEEVRLVVSLDESKASNDILSLANQIAEMSDLITVERDENASA
CCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCC
RKPVMAVTNPAKGTALRFAGLPMGHEFTSLVLALLHSGGHPIKLEPEVIEQIAALDKDLV
CCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEECHHHHHHHHHCCHHHH
VEIFISLSCQNCPDVVQAFNMMAAINPKVKATMIDGGLFQDEVKQRDIMAVPSVFVNGEL
HHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCEECCCEEECCEE
FGQGRMSLTEILNKVDDGASEKAAKALSEKDPYDVLVVGGGPGGSAAALYAARKGIRTGV
ECCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHCCHHHH
VAKRFGGQVMDTMAIENFISVKRTEGPKLATDLAEHLKEYDVDVVTEQQAEKLMGAAHTE
HHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEECHHHHHHHHCCCCCC
DGLIHIQLESGATLKSKSVILSPGARWREMNVPGEQEYRNKGVAYCPHCDGPLFKGKKTA
CCEEEEEECCCCCEECCEEEECCCCCEEECCCCCHHHHHCCCCEECCCCCCCCCCCCEEE
VIGGGNSGIEAAIDLAGIVEHVTVLEFADVLRADQVLIDKANSLPNVDIITMAQTTEVVG
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEHHHHHC
DGTRVTSLKYKDRNTDEIKEIELSGIFVQIGLVPNTEWLKDSEVALSERGEIEVGSRGDT
CCCEEEEEEECCCCCCHHHEEEEEEEEEEEEECCCCCCCCCCCHHHCCCCCEEECCCCCC
SLEGVFAAGDATTIPYKQIIIAMGEGAKASLGAFDYLIRKQG
CCCEEEECCCCCCCCHHHEEEEECCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]