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The map label for this gene is rspA [H]

Identifier: 156936592

GI number: 156936592

Start: 37306

End: 38652

Strand: Direct

Name: rspA [H]

Synonym: ESA_pESA3p05473

Alternate gene names: 156936592

Gene position: 37306-38652 (Clockwise)

Preceding gene: 156936590

Following gene: 156936595

Centisome position: 28.44

GC content: 55.83

Gene sequence:

>1347_bases
ATGGTAAAATTGGTATGGTTTATTGGTGCTTTTAAAAAGAAATTACTGACCAAAATAAATAGTCAAAAAAAGGGTTTACC
CGCCATTATTTTTAAGGTCATGATATTGACATTCCGTATTTCCATAGCGCTGCAAAGGAGTGATTTCGTGACGCCTGTGA
ACATCAAAAATATCGAATGTTTTATCACCCGCCCGGACAGGCATAACCTGGTGACGGTTCGCGTGACGACCGACCAGGGC
GTGACGGGGCACGGCTGCGCCACGTTCCAGCAGCGCCCGCTCGCTGTTAAAACGCTGGTGGAGGAGTATTTACAGCCGCT
GCTGACGGGCCGTGATGCGAACAATATCGAAGATCTGTGGCAGATGATGAACGTCAACGCCTACTGGCGTAACGGCCCGG
TGATGAACAACGCAATATCGGGCGTGGATATGGCGCTGTGGGATATTAAAAGCCAGCTCGCCGGTATGCCGCTGTACCAG
CTTTTCGGCGGTAAATCCCGTGACGCGATCCCGGCATACAGCCACGCCAGCGGCGACACGCTGGACGCGCTTTTTGCGTC
CGTGGATGCGCTCATCGAACAGGGATATCGCCATATTCGCTGCCAGCTCGGTTTTTACGGCGGCACGCCTTCCGGCCTGC
ACGCGCCGGAAAACCCGACGCCCGGCGCGTGGTTCGATCAGCAGGAATATATGCGCAATACCGTCGATATGTTCCGCGCG
CTGCGTGAGAAATATGGCTGGAGCCTGCATTTTCTGCATGATGTTCACGAGCGTCTGTTCCCGCAGCAGGCCATACAGCT
TGCGAAACAACTGGAGCCTTACCAGCCCTATTTTATTGAAGATATTCTGCCTCCCCAGCAGAGCGCCTGGCTTGAACAGG
TACGCCAGCATAGCTGCGTGCCGCTGGCGATGGGCGAGCTCTTTAATAACCCAAGTGAATGGCACGATCTGATCGTCAAC
CGCCGTATCGATTTTATCCGCTGCCACGTTTCGCAAATCGGCGGGATCACCCCGGCACTGAAGCTTGCGCATCTGTGCCA
GGCGTTCGGTGTGCGTCTCGCGTGGCATGGCCCGGGCGATATGACCCCGATTGGCGTGGCGGTGAACACGCATTTAAACA
TTCATCTGCATAACGCGGCGATTCAGGAATTTATTCCGCGCTCGGCCATGACCGATCGGGTTTTCCCCGGCGCGCCGGAG
GTGAAAGACGGGTTTATCTATCCGCCGGTCAATGCCGGCATCGGCGTCGGATTTAACGAGGCGCTGGCGCAGGCGCATCC
GGTGATGTATCGCCCGCATGAATGGACACAAAGCCGCCTGCCGGACGGCACGCTTCACACGCCCTGA

Upstream 100 bases:

>100_bases
ATGGTCCTGTGAAGGATAATTAAACCTTTTTGACAAAACAAACCAATAAAAGTTGATTCTGCTCACATCTTTGCTTCTGG
TGTGGATTGAAAGCAGTGTA

Downstream 100 bases:

>100_bases
TGCTGGCGGCGGGTCAGCGTGTCGCGGTTATACGGAATGACATAGGTGCAGGTGTAGTTGATATCAATAATATCGCTCAC
CTCAAACACGCGACCGCCTT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MVKLVWFIGAFKKKLLTKINSQKKGLPAIIFKVMILTFRISIALQRSDFVTPVNIKNIECFITRPDRHNLVTVRVTTDQG
VTGHGCATFQQRPLAVKTLVEEYLQPLLTGRDANNIEDLWQMMNVNAYWRNGPVMNNAISGVDMALWDIKSQLAGMPLYQ
LFGGKSRDAIPAYSHASGDTLDALFASVDALIEQGYRHIRCQLGFYGGTPSGLHAPENPTPGAWFDQQEYMRNTVDMFRA
LREKYGWSLHFLHDVHERLFPQQAIQLAKQLEPYQPYFIEDILPPQQSAWLEQVRQHSCVPLAMGELFNNPSEWHDLIVN
RRIDFIRCHVSQIGGITPALKLAHLCQAFGVRLAWHGPGDMTPIGVAVNTHLNIHLHNAAIQEFIPRSAMTDRVFPGAPE
VKDGFIYPPVNAGIGVGFNEALAQAHPVMYRPHEWTQSRLPDGTLHTP

Sequences:

>Translated_448_residues
MVKLVWFIGAFKKKLLTKINSQKKGLPAIIFKVMILTFRISIALQRSDFVTPVNIKNIECFITRPDRHNLVTVRVTTDQG
VTGHGCATFQQRPLAVKTLVEEYLQPLLTGRDANNIEDLWQMMNVNAYWRNGPVMNNAISGVDMALWDIKSQLAGMPLYQ
LFGGKSRDAIPAYSHASGDTLDALFASVDALIEQGYRHIRCQLGFYGGTPSGLHAPENPTPGAWFDQQEYMRNTVDMFRA
LREKYGWSLHFLHDVHERLFPQQAIQLAKQLEPYQPYFIEDILPPQQSAWLEQVRQHSCVPLAMGELFNNPSEWHDLIVN
RRIDFIRCHVSQIGGITPALKLAHLCQAFGVRLAWHGPGDMTPIGVAVNTHLNIHLHNAAIQEFIPRSAMTDRVFPGAPE
VKDGFIYPPVNAGIGVGFNEALAQAHPVMYRPHEWTQSRLPDGTLHTP
>Mature_448_residues
MVKLVWFIGAFKKKLLTKINSQKKGLPAIIFKVMILTFRISIALQRSDFVTPVNIKNIECFITRPDRHNLVTVRVTTDQG
VTGHGCATFQQRPLAVKTLVEEYLQPLLTGRDANNIEDLWQMMNVNAYWRNGPVMNNAISGVDMALWDIKSQLAGMPLYQ
LFGGKSRDAIPAYSHASGDTLDALFASVDALIEQGYRHIRCQLGFYGGTPSGLHAPENPTPGAWFDQQEYMRNTVDMFRA
LREKYGWSLHFLHDVHERLFPQQAIQLAKQLEPYQPYFIEDILPPQQSAWLEQVRQHSCVPLAMGELFNNPSEWHDLIVN
RRIDFIRCHVSQIGGITPALKLAHLCQAFGVRLAWHGPGDMTPIGVAVNTHLNIHLHNAAIQEFIPRSAMTDRVFPGAPE
VKDGFIYPPVNAGIGVGFNEALAQAHPVMYRPHEWTQSRLPDGTLHTP

Specific function: Probably involved in the degradation of homoserine lactone (HSL) or of a metabolite of HSL that signals starvation [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787864, Length=404, Percent_Identity=38.1188118811881, Blast_Score=289, Evalue=2e-79,
Organism=Escherichia coli, GI48994953, Length=361, Percent_Identity=31.3019390581717, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI226510960, Length=322, Percent_Identity=25.776397515528, Blast_Score=89, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 50525; Mature: 50525

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKLVWFIGAFKKKLLTKINSQKKGLPAIIFKVMILTFRISIALQRSDFVTPVNIKNIEC
CEEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCEEE
FITRPDRHNLVTVRVTTDQGVTGHGCATFQQRPLAVKTLVEEYLQPLLTGRDANNIEDLW
EEECCCCCCEEEEEEECCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHH
QMMNVNAYWRNGPVMNNAISGVDMALWDIKSQLAGMPLYQLFGGKSRDAIPAYSHASGDT
HHHCCCEEECCCCCCCHHHCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCH
LDALFASVDALIEQGYRHIRCQLGFYGGTPSGLHAPENPTPGAWFDQQEYMRNTVDMFRA
HHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LREKYGWSLHFLHDVHERLFPQQAIQLAKQLEPYQPYFIEDILPPQQSAWLEQVRQHSCV
HHHHHCCEEHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHCCCC
PLAMGELFNNPSEWHDLIVNRRIDFIRCHVSQIGGITPALKLAHLCQAFGVRLAWHGPGD
CEEHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEECCCC
MTPIGVAVNTHLNIHLHNAAIQEFIPRSAMTDRVFPGAPEVKDGFIYPPVNAGIGVGFNE
CCEEEEEEEEEEEEEEEHHHHHHHHCHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCHHH
ALAQAHPVMYRPHEWTQSRLPDGTLHTP
HHHHCCCCEECCCHHHHHCCCCCCCCCC
>Mature Secondary Structure
MVKLVWFIGAFKKKLLTKINSQKKGLPAIIFKVMILTFRISIALQRSDFVTPVNIKNIEC
CEEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCEEE
FITRPDRHNLVTVRVTTDQGVTGHGCATFQQRPLAVKTLVEEYLQPLLTGRDANNIEDLW
EEECCCCCCEEEEEEECCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHH
QMMNVNAYWRNGPVMNNAISGVDMALWDIKSQLAGMPLYQLFGGKSRDAIPAYSHASGDT
HHHCCCEEECCCCCCCHHHCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCH
LDALFASVDALIEQGYRHIRCQLGFYGGTPSGLHAPENPTPGAWFDQQEYMRNTVDMFRA
HHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LREKYGWSLHFLHDVHERLFPQQAIQLAKQLEPYQPYFIEDILPPQQSAWLEQVRQHSCV
HHHHHCCEEHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHCCCC
PLAMGELFNNPSEWHDLIVNRRIDFIRCHVSQIGGITPALKLAHLCQAFGVRLAWHGPGD
CEEHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEECCCC
MTPIGVAVNTHLNIHLHNAAIQEFIPRSAMTDRVFPGAPEVKDGFIYPPVNAGIGVGFNE
CCEEEEEEEEEEEEEEEHHHHHHHHCHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCHHH
ALAQAHPVMYRPHEWTQSRLPDGTLHTP
HHHHCCCCEECCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7545940; 9097039; 9278503 [H]