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The map label for this gene is pla [H]

Identifier: 156936553

GI number: 156936553

Start: 6101

End: 6916

Strand: Direct

Name: pla [H]

Synonym: ESA_pESA3p05434

Alternate gene names: 156936553

Gene position: 6101-6916 (Clockwise)

Preceding gene: 156936551

Following gene: 156936554

Centisome position: 4.65

GC content: 48.16

Gene sequence:

>816_bases
ATGCTGGCAGGGGAGTCGAAAGAATTTGTATATGACTCCAGTACAGGTCGTAAAATCAGTCAGCTGAACTGGAAAATTAA
AAATAATCTCATCCTTAAAGGCGATGTTTCCTGGGATGCATGGCCTTTCCTGACCCTGAATGCGCGCGGCTGGACAACCC
TGAGTTCTGGTAACGGGCATATGGAAGATTATGACTGGCAAAAAGAAGATCAGTCTCACTGGACCGACCGTTCGCGCCAT
CCTGATACCGATCTGAATTATGCCAATGAATATGATTTAAACCTGAAAGGCTGGGTACTGAAAGGGGATGATTATAAAGT
CGGCGTTATGGCCGGTTATCAACAAACCCGTTTCAGCTGGACCGCCTATGGCGGCTCCTTTAACTATGCGAATGGCAATG
ATGTTGGCAGCTTCCCGAACGGTGCCCGCGGTATCGGTTACCAGCAGAAATACAGCATGCCGTATATTGGCCTGACCGGT
CAGTATCGTTATCAGGACTTTGAAGTGAACGCGCAGTTTAAATTCAGCGACTGGGTGACCGCGAAGGATAACGACGAGCA
TTATATGCGCGATCTGACGTTCCGTGAAAAAGCTAACGACTCCCGTTACTATGGCCTCGCCGTTGATGCCGGTTATTATG
TGCTCCCGAATACGAAACTTTTCGCTGAGTTCAGCTATAACCGCTATGAGGAAGGGAAAAGCGGTACGCAGATGATTAAT
AACGCGACCGGAACGTCTTTTTCGGATAATGGCGATGCCGCCGGCCTGCAAAACAAAAACTATACAATTACCGCAGGTCT
TCAGTACCGTTTCTGA

Upstream 100 bases:

>100_bases
GGCGATGATAGCTGCCTCCGGCTCGGCGAATGCCGCTTCTTCTGTAACACTGATGCCGGATTTTTCTGCGCAAAGCGTGG
CTGTCTCCACATCACTGGGG

Downstream 100 bases:

>100_bases
TGCCGTTAAAAAACCCGCTGCCGGCGGGTTTTTTTCACACATAACCTGTACCGATAACCTCGTCAACATTTCTTAATCCC
TTTCCTCGTGGCTGTTCCCC

Product: outer membrane protease

Products: NA

Alternate protein names: Plasminogen activator [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MLAGESKEFVYDSSTGRKISQLNWKIKNNLILKGDVSWDAWPFLTLNARGWTTLSSGNGHMEDYDWQKEDQSHWTDRSRH
PDTDLNYANEYDLNLKGWVLKGDDYKVGVMAGYQQTRFSWTAYGGSFNYANGNDVGSFPNGARGIGYQQKYSMPYIGLTG
QYRYQDFEVNAQFKFSDWVTAKDNDEHYMRDLTFREKANDSRYYGLAVDAGYYVLPNTKLFAEFSYNRYEEGKSGTQMIN
NATGTSFSDNGDAAGLQNKNYTITAGLQYRF

Sequences:

>Translated_271_residues
MLAGESKEFVYDSSTGRKISQLNWKIKNNLILKGDVSWDAWPFLTLNARGWTTLSSGNGHMEDYDWQKEDQSHWTDRSRH
PDTDLNYANEYDLNLKGWVLKGDDYKVGVMAGYQQTRFSWTAYGGSFNYANGNDVGSFPNGARGIGYQQKYSMPYIGLTG
QYRYQDFEVNAQFKFSDWVTAKDNDEHYMRDLTFREKANDSRYYGLAVDAGYYVLPNTKLFAEFSYNRYEEGKSGTQMIN
NATGTSFSDNGDAAGLQNKNYTITAGLQYRF
>Mature_271_residues
MLAGESKEFVYDSSTGRKISQLNWKIKNNLILKGDVSWDAWPFLTLNARGWTTLSSGNGHMEDYDWQKEDQSHWTDRSRH
PDTDLNYANEYDLNLKGWVLKGDDYKVGVMAGYQQTRFSWTAYGGSFNYANGNDVGSFPNGARGIGYQQKYSMPYIGLTG
QYRYQDFEVNAQFKFSDWVTAKDNDEHYMRDLTFREKANDSRYYGLAVDAGYYVLPNTKLFAEFSYNRYEEGKSGTQMIN
NATGTSFSDNGDAAGLQNKNYTITAGLQYRF

Specific function: Seems to play an essential role in plague transmission by mediating flea blockage in a temperature-dependent fashion. Fibrinolytic activity prevails at 37 degrees Celsius whereas coagulase expression predominates at lower temperatures (<30 degrees Celsius

COG id: COG4571

COG function: function code M; Outer membrane protease

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A26 family [H]

Homologues:

Organism=Escherichia coli, GI1786777, Length=279, Percent_Identity=51.2544802867383, Blast_Score=283, Evalue=6e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020080
- InterPro:   IPR000036
- InterPro:   IPR020079 [H]

Pfam domain/function: PF01278 Omptin [H]

EC number: =3.4.23.48 [H]

Molecular weight: Translated: 31085; Mature: 31085

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00834 OMPTIN_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAGESKEFVYDSSTGRKISQLNWKIKNNLILKGDVSWDAWPFLTLNARGWTTLSSGNGH
CCCCCCCCEEEECCCCCEEEEEEEEEECCEEEEECCCCCCCEEEEEECCCCEEEECCCCC
MEDYDWQKEDQSHWTDRSRHPDTDLNYANEYDLNLKGWVLKGDDYKVGVMAGYQQTRFSW
CCCCCCCCCCHHHCCCCCCCCCCCCCCCCEECCCEEEEEEECCCEEEEEEECCCEEEEEE
TAYGGSFNYANGNDVGSFPNGARGIGYQQKYSMPYIGLTGQYRYQDFEVNAQFKFSDWVT
EEECCEEECCCCCCCCCCCCCCCCCCCCHHCCCCEEECCCCEEEEEEEEEEEEEECCEEE
AKDNDEHYMRDLTFREKANDSRYYGLAVDAGYYVLPNTKLFAEFSYNRYEEGKSGTQMIN
ECCCCHHHHHHHHHHHCCCCCEEEEEEEECCEEEECCCEEEEEECCCCCCCCCCCHHHHH
NATGTSFSDNGDAAGLQNKNYTITAGLQYRF
CCCCCCCCCCCCCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MLAGESKEFVYDSSTGRKISQLNWKIKNNLILKGDVSWDAWPFLTLNARGWTTLSSGNGH
CCCCCCCCEEEECCCCCEEEEEEEEEECCEEEEECCCCCCCEEEEEECCCCEEEECCCCC
MEDYDWQKEDQSHWTDRSRHPDTDLNYANEYDLNLKGWVLKGDDYKVGVMAGYQQTRFSW
CCCCCCCCCCHHHCCCCCCCCCCCCCCCCEECCCEEEEEEECCCEEEEEEECCCEEEEEE
TAYGGSFNYANGNDVGSFPNGARGIGYQQKYSMPYIGLTGQYRYQDFEVNAQFKFSDWVT
EEECCEEECCCCCCCCCCCCCCCCCCCCHHCCCCEEECCCCEEEEEEEEEEEEEECCEEE
AKDNDEHYMRDLTFREKANDSRYYGLAVDAGYYVLPNTKLFAEFSYNRYEEGKSGTQMIN
ECCCCHHHHHHHHHHHCCCCCEEEEEEEECCEEEECCCEEEEEECCCCCCCCCCCHHHHH
NATGTSFSDNGDAAGLQNKNYTITAGLQYRF
CCCCCCCCCCCCCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2526282; 2651310; 9748454; 11586360 [H]