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The map label for this gene is thiI [C]

Identifier: 156938122

GI number: 156938122

Start: 1187326

End: 1188444

Strand: Reverse

Name: thiI [C]

Synonym: Igni_1335

Alternate gene names: 156938122

Gene position: 1188444-1187326 (Counterclockwise)

Preceding gene: 156938126

Following gene: 156938120

Centisome position: 91.59

GC content: 55.23

Gene sequence:

>1119_bases
TTGACGTGCGCCATACTGGCGAAGACCGATATTGGCATGGAAAAGTACGTAGCTAACGTACTCAGGGTGCCGTGTAAGAA
GGTAGTCCCGGCCCCTATGGGGTTCAAGGGCCTCATTCTAATAGAGGAGTGTGAGGACTTCGAGAAGGCCTTTCAAGTAG
TTTCCAAAGTACCGGAAGTCATTAGGGTAATGAAGGCCGAAAGGTGCGTTAGGGCCGATCTGAACGAGCTGAAGAGAGTT
GCGGAAGAGATGGCCAGTAGACTGAAAGGTAAGAGGTTTGCCGTAAGGACCGTGAGGAGGGGAAAACACCCATTCACTTC
GGTTCAAGTGAACGCGGAGCTGGGGTCCGTCGTACTGTCCAAGGTAGACGCCGAAGTAGACCTTTCCGACCCAGAGGCGG
TGCTTATGGTAGAGATAGTGGGAGATAGAGCCTTCGTTTCTATAGTCCCTCCCGAGTATTACGCGTTAAAGAAAAAGAAA
GGAAAGGTAGACGTGTCAGAAGTCATTAGAAAGGTGGTAGTCGTCCAAGAGCCCTACTTAGGCCCTTTGGAGGGCGTCAA
GGAGGTGTCCAAGAGAGTCGGGAGGGTGCTACAGAGCTTCGGCGTCGGGACGTACTACGCCGCCCTCACTGAGGAGGCCG
ACGGCGACCAGCTGGCGGAGTTCTTGAGAGGGGTTAGGGAGGGGGCCGAGTCCAGGAGGAGGCAGAGGGAGAAGAGCGAG
GGTAAGGCTAATAGGTTGCAAATAAAGGTGTTTGACATGTACCATTTAGTAGCTTCGAGGGGGAAGAAGGACTTAGTTGT
GGTATTCGAGCCAGAAGGCAAGAGCTTCGAAGAAGTAATGGAAGATCTAGGAAGGGCCTTGAGGAAGGCTAGGAGGGTCT
ACCTAGTCCTAGGGTCTAGGAAAGGAGTACCCATGGGCTTATACAAGTTTGCGGACTTCGTGGTCGACGTGGCCCCGGGG
GCGGTCCTCTCCACGGAAACGGCCGTGGCCGCCGCCTTGGAGGCGGTCGTCATAGCTTTCTTGGCCTCACAAAGGTCCGA
CGTCGGGCCAGCCTTGCCTCTGTCCGGTTCCCGCGAGGCCGGTGAGGGCTTGGGGCCCCTTAAGGATCAAGAGGGCTAA

Upstream 100 bases:

>100_bases
AGCGGGAGCTTTTCCAACAATATCGTGTGGCCCCACGCCGCTCCTCGGGGGTTCACTATTTTTCGTTGTCCCTCCGCAGC
GCGGCTCAAGGGGTATCGCT

Downstream 100 bases:

>100_bases
GTTCTCCGCGTGTTCCTTTGCCCTCTGTACGGCTATTTCCTTCAGCTTCCGCGGGTCGTCGGTCTCGTCCTCGTGCACAG
TTACATCTATTATGTGTTTT

Product: THUMP domain-containing protein

Products: ThiS-COSH; AMP [C]

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MTCAILAKTDIGMEKYVANVLRVPCKKVVPAPMGFKGLILIEECEDFEKAFQVVSKVPEVIRVMKAERCVRADLNELKRV
AEEMASRLKGKRFAVRTVRRGKHPFTSVQVNAELGSVVLSKVDAEVDLSDPEAVLMVEIVGDRAFVSIVPPEYYALKKKK
GKVDVSEVIRKVVVVQEPYLGPLEGVKEVSKRVGRVLQSFGVGTYYAALTEEADGDQLAEFLRGVREGAESRRRQREKSE
GKANRLQIKVFDMYHLVASRGKKDLVVVFEPEGKSFEEVMEDLGRALRKARRVYLVLGSRKGVPMGLYKFADFVVDVAPG
AVLSTETAVAAALEAVVIAFLASQRSDVGPALPLSGSREAGEGLGPLKDQEG

Sequences:

>Translated_372_residues
MTCAILAKTDIGMEKYVANVLRVPCKKVVPAPMGFKGLILIEECEDFEKAFQVVSKVPEVIRVMKAERCVRADLNELKRV
AEEMASRLKGKRFAVRTVRRGKHPFTSVQVNAELGSVVLSKVDAEVDLSDPEAVLMVEIVGDRAFVSIVPPEYYALKKKK
GKVDVSEVIRKVVVVQEPYLGPLEGVKEVSKRVGRVLQSFGVGTYYAALTEEADGDQLAEFLRGVREGAESRRRQREKSE
GKANRLQIKVFDMYHLVASRGKKDLVVVFEPEGKSFEEVMEDLGRALRKARRVYLVLGSRKGVPMGLYKFADFVVDVAPG
AVLSTETAVAAALEAVVIAFLASQRSDVGPALPLSGSREAGEGLGPLKDQEG
>Mature_371_residues
TCAILAKTDIGMEKYVANVLRVPCKKVVPAPMGFKGLILIEECEDFEKAFQVVSKVPEVIRVMKAERCVRADLNELKRVA
EEMASRLKGKRFAVRTVRRGKHPFTSVQVNAELGSVVLSKVDAEVDLSDPEAVLMVEIVGDRAFVSIVPPEYYALKKKKG
KVDVSEVIRKVVVVQEPYLGPLEGVKEVSKRVGRVLQSFGVGTYYAALTEEADGDQLAEFLRGVREGAESRRRQREKSEG
KANRLQIKVFDMYHLVASRGKKDLVVVFEPEGKSFEEVMEDLGRALRKARRVYLVLGSRKGVPMGLYKFADFVVDVAPGA
VLSTETAVAAALEAVVIAFLASQRSDVGPALPLSGSREAGEGLGPLKDQEG

Specific function: Required For The Synthesis Of The Thiazole Moiety. [C]

COG id: COG1818

COG function: function code R; Predicted RNA-binding protein, contains THUMP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004114 [H]

Pfam domain/function: PF02926 THUMP [H]

EC number: NA

Molecular weight: Translated: 40825; Mature: 40694

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS51165 THUMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCAILAKTDIGMEKYVANVLRVPCKKVVPAPMGFKGLILIEECEDFEKAFQVVSKVPEV
CCEEEEECCCCCHHHHHHHHHHCCHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
IRVMKAERCVRADLNELKRVAEEMASRLKGKRFAVRTVRRGKHPFTSVQVNAELGSVVLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEECHHHHHHHHH
KVDAEVDLSDPEAVLMVEIVGDRAFVSIVPPEYYALKKKKGKVDVSEVIRKVVVVQEPYL
HHCCCCCCCCCCEEEEEEEECCCEEEEECCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCC
GPLEGVKEVSKRVGRVLQSFGVGTYYAALTEEADGDQLAEFLRGVREGAESRRRQREKSE
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GKANRLQIKVFDMYHLVASRGKKDLVVVFEPEGKSFEEVMEDLGRALRKARRVYLVLGSR
CCCCEEEEEEHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCEEEEEEECC
KGVPMGLYKFADFVVDVAPGAVLSTETAVAAALEAVVIAFLASQRSDVGPALPLSGSREA
CCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
GEGLGPLKDQEG
CCCCCCCCCCCC
>Mature Secondary Structure 
TCAILAKTDIGMEKYVANVLRVPCKKVVPAPMGFKGLILIEECEDFEKAFQVVSKVPEV
CEEEEECCCCCHHHHHHHHHHCCHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
IRVMKAERCVRADLNELKRVAEEMASRLKGKRFAVRTVRRGKHPFTSVQVNAELGSVVLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEECHHHHHHHHH
KVDAEVDLSDPEAVLMVEIVGDRAFVSIVPPEYYALKKKKGKVDVSEVIRKVVVVQEPYL
HHCCCCCCCCCCEEEEEEEECCCEEEEECCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCC
GPLEGVKEVSKRVGRVLQSFGVGTYYAALTEEADGDQLAEFLRGVREGAESRRRQREKSE
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GKANRLQIKVFDMYHLVASRGKKDLVVVFEPEGKSFEEVMEDLGRALRKARRVYLVLGSR
CCCCEEEEEEHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCEEEEEEECC
KGVPMGLYKFADFVVDVAPGAVLSTETAVAAALEAVVIAFLASQRSDVGPALPLSGSREA
CCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
GEGLGPLKDQEG
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]