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The map label for this gene is leuA2 [H]

Identifier: 156938036

GI number: 156938036

Start: 1108645

End: 1110078

Strand: Direct

Name: leuA2 [H]

Synonym: Igni_1249

Alternate gene names: 156938036

Gene position: 1108645-1110078 (Clockwise)

Preceding gene: 156938032

Following gene: 156938038

Centisome position: 85.44

GC content: 58.44

Gene sequence:

>1434_bases
TTGGTGACGCCGTTCTCAGATGAGCTGAGAAAGTACAGGCCGGCGTTCGAACGGGTTAAGATACTTGACGCCACCTTGAG
GGAGGGGGAGCAGACCCCGGGCGTCTCCTTCACTATAGACCAGAAGCTGGAGATAGCGAAGAAGCTGGACGAGATAGGCG
TCCACATGATAGAGGCCGGCCACCCGGCGGTGGCGCCGGACGTGTACGAGGCCGTGAGGAGGATAGCGTGGATGAAGAAG
GAGGGGGAGATAAGGGCCGAGGTCGTGGCCCACAGCAGGGCGGTCAAGAGCGACATAGACAAGGCCGCGGAGACCGAAGC
GGATAGGATAGCCATCTTCTACGGGGTCAGCGACATACACTTGAACGCTAAACACAAGAAGACGAGGGAAGAGGCCCTCA
GCATAATCGGCGAAATGGTGGAGTACGCGAAGGGTTACGGCGTGAAGGTAAGGTTCACCGCCGAGGACGCCTCGAGGGCG
GACTTGCAGTACTTGGAGCAAGTGGTCAAGACCGCGAGGGACGCGGGGGCGGACAGGGTGAGCTTGGCCGACACGGTAGG
GGTACTAACCCCGTGGACCGCTCAGAAGTTCTTCGAACACATGGTAAAGGCAGTCCCTAACGTTGAATACGACATCCACG
CTCACAATGACTTAGGCATGGCGCTGGCCACGGCGTTCGGGGCGGTAATGGGAGGGGCCACGGCAGTACACGTGACAGTA
AACGGGCTGGGCGAGAGGGTGGGCATAGTCCCCCTCCAGCTCTTCGCGGTGGCGGTGAAGGTACACTTCGACGTGGAGCT
GGTGAAGCTGAGCGGCCTGATGGAGTTGACGAGGATGGTCGAGCGCTACTCCGGCATAGAGGTCCCCTACAACATGCCGG
TCATAGGCGACTACGCGTTCTTGCACAAGGCCGGCGTCCACGTGGCCGGCATACTGGCAGACCCGAGGACCTACGAGGCC
TTCCCGCCGGAGATGATAGGGAGGAGCAGGGACTACGTGATAGACAAATACACCGGAAAGAAGGCAGTGAAGGCGAAGCT
CGAGAAGCTCGGAATAAAGGTGGACGACGACACGCTGATGAAGATACTAGAAGAGATAAAGAGGAGCAACTTAAGGTTCC
TCACCGACGACGCGTTGATAAGCATCGCTGAGAAGGTGAGCGGCAAGGTGCTCAGGCCCAAGGCGACGGAGAACGTGGAG
GCGGTGGTCTGGGTGAAGGTGGACAGCAACGCCTTTACGGGCTCGGTGGCCCGGAGGCTGAGCAACATAGAGGGCGTTAA
GGAGGTTTCCGAAGTAACTGGCGACTATGACTTGCTAGTGAGGATAGAGGCCCCAGACCACAAAGTGCTAAATACCATAC
TGGACACCGTGAGGGGCATGAAGGGTATAGTAGGTACCCACACCCAAATAGTCCTCAAGAAGCTCGAGAAGTGA

Upstream 100 bases:

>100_bases
CGGTCACTGCGCGATCCCGGTCGGACTCGGGGAGGAGGTTATATAGTGTTGCTGCAAAAACCCTATTATTTCCTACTAAG
CGCGCGTCCGGTAGTCGGCT

Downstream 100 bases:

>100_bases
ACCCTCTCTCCTTCAACAACTCCTCCTTAGCCTTCTCTATAGCTAGATCGAGCGCGAGCCTCTTTATCATATTTTCTAAC
TCAGAGGCGCTCAAGGGGCC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2 [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MVTPFSDELRKYRPAFERVKILDATLREGEQTPGVSFTIDQKLEIAKKLDEIGVHMIEAGHPAVAPDVYEAVRRIAWMKK
EGEIRAEVVAHSRAVKSDIDKAAETEADRIAIFYGVSDIHLNAKHKKTREEALSIIGEMVEYAKGYGVKVRFTAEDASRA
DLQYLEQVVKTARDAGADRVSLADTVGVLTPWTAQKFFEHMVKAVPNVEYDIHAHNDLGMALATAFGAVMGGATAVHVTV
NGLGERVGIVPLQLFAVAVKVHFDVELVKLSGLMELTRMVERYSGIEVPYNMPVIGDYAFLHKAGVHVAGILADPRTYEA
FPPEMIGRSRDYVIDKYTGKKAVKAKLEKLGIKVDDDTLMKILEEIKRSNLRFLTDDALISIAEKVSGKVLRPKATENVE
AVVWVKVDSNAFTGSVARRLSNIEGVKEVSEVTGDYDLLVRIEAPDHKVLNTILDTVRGMKGIVGTHTQIVLKKLEK

Sequences:

>Translated_477_residues
MVTPFSDELRKYRPAFERVKILDATLREGEQTPGVSFTIDQKLEIAKKLDEIGVHMIEAGHPAVAPDVYEAVRRIAWMKK
EGEIRAEVVAHSRAVKSDIDKAAETEADRIAIFYGVSDIHLNAKHKKTREEALSIIGEMVEYAKGYGVKVRFTAEDASRA
DLQYLEQVVKTARDAGADRVSLADTVGVLTPWTAQKFFEHMVKAVPNVEYDIHAHNDLGMALATAFGAVMGGATAVHVTV
NGLGERVGIVPLQLFAVAVKVHFDVELVKLSGLMELTRMVERYSGIEVPYNMPVIGDYAFLHKAGVHVAGILADPRTYEA
FPPEMIGRSRDYVIDKYTGKKAVKAKLEKLGIKVDDDTLMKILEEIKRSNLRFLTDDALISIAEKVSGKVLRPKATENVE
AVVWVKVDSNAFTGSVARRLSNIEGVKEVSEVTGDYDLLVRIEAPDHKVLNTILDTVRGMKGIVGTHTQIVLKKLEK
>Mature_477_residues
MVTPFSDELRKYRPAFERVKILDATLREGEQTPGVSFTIDQKLEIAKKLDEIGVHMIEAGHPAVAPDVYEAVRRIAWMKK
EGEIRAEVVAHSRAVKSDIDKAAETEADRIAIFYGVSDIHLNAKHKKTREEALSIIGEMVEYAKGYGVKVRFTAEDASRA
DLQYLEQVVKTARDAGADRVSLADTVGVLTPWTAQKFFEHMVKAVPNVEYDIHAHNDLGMALATAFGAVMGGATAVHVTV
NGLGERVGIVPLQLFAVAVKVHFDVELVKLSGLMELTRMVERYSGIEVPYNMPVIGDYAFLHKAGVHVAGILADPRTYEA
FPPEMIGRSRDYVIDKYTGKKAVKAKLEKLGIKVDDDTLMKILEEIKRSNLRFLTDDALISIAEKVSGKVLRPKATENVE
AVVWVKVDSNAFTGSVARRLSNIEGVKEVSEVTGDYDLLVRIEAPDHKVLNTILDTVRGMKGIVGTHTQIVLKKLEK

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=349, Percent_Identity=33.2378223495702, Blast_Score=194, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6320019, Length=397, Percent_Identity=31.7380352644836, Blast_Score=203, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6320071, Length=400, Percent_Identity=31.5, Blast_Score=200, Evalue=4e-52,
Organism=Drosophila melanogaster, GI24582381, Length=247, Percent_Identity=25.1012145748988, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011008
- InterPro:   IPR011872
- InterPro:   IPR000891
- InterPro:   IPR019887 [H]

Pfam domain/function: PF01037 AsnC_trans_reg; PF00682 HMGL-like [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 52727; Mature: 52727

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTPFSDELRKYRPAFERVKILDATLREGEQTPGVSFTIDQKLEIAKKLDEIGVHMIEAG
CCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHCCEEEECC
HPAVAPDVYEAVRRIAWMKKEGEIRAEVVAHSRAVKSDIDKAAETEADRIAIFYGVSDIH
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEE
LNAKHKKTREEALSIIGEMVEYAKGYGVKVRFTAEDASRADLQYLEQVVKTARDAGADRV
ECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCE
SLADTVGVLTPWTAQKFFEHMVKAVPNVEYDIHAHNDLGMALATAFGAVMGGATAVHVTV
EHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEE
NGLGERVGIVPLQLFAVAVKVHFDVELVKLSGLMELTRMVERYSGIEVPYNMPVIGDYAF
ECCCCCCCHHHHHHHHHHHHHEECEEEHHHHHHHHHHHHHHHHCCCCCCCCCCEECCHHH
LHKAGVHVAGILADPRTYEAFPPEMIGRSRDYVIDKYTGKKAVKAKLEKLGIKVDDDTLM
HHHCCCEEEEEEECCCCCCCCCHHHHCCCCCCEEECCCCHHHHHHHHHHCCCEECHHHHH
KILEEIKRSNLRFLTDDALISIAEKVSGKVLRPKATENVEAVVWVKVDSNAFTGSVARRL
HHHHHHHHCCCEEEEHHHHHHHHHHHCCCEECCCCCCCCEEEEEEEECCCCCHHHHHHHH
SNIEGVKEVSEVTGDYDLLVRIEAPDHKVLNTILDTVRGMKGIVGTHTQIVLKKLEK
HHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MVTPFSDELRKYRPAFERVKILDATLREGEQTPGVSFTIDQKLEIAKKLDEIGVHMIEAG
CCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHCCEEEECC
HPAVAPDVYEAVRRIAWMKKEGEIRAEVVAHSRAVKSDIDKAAETEADRIAIFYGVSDIH
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEE
LNAKHKKTREEALSIIGEMVEYAKGYGVKVRFTAEDASRADLQYLEQVVKTARDAGADRV
ECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCE
SLADTVGVLTPWTAQKFFEHMVKAVPNVEYDIHAHNDLGMALATAFGAVMGGATAVHVTV
EHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEE
NGLGERVGIVPLQLFAVAVKVHFDVELVKLSGLMELTRMVERYSGIEVPYNMPVIGDYAF
ECCCCCCCHHHHHHHHHHHHHEECEEEHHHHHHHHHHHHHHHHCCCCCCCCCCEECCHHH
LHKAGVHVAGILADPRTYEAFPPEMIGRSRDYVIDKYTGKKAVKAKLEKLGIKVDDDTLM
HHHCCCEEEEEEECCCCCCCCCHHHHCCCCCCEEECCCCHHHHHHHHHHCCCEECHHHHH
KILEEIKRSNLRFLTDDALISIAEKVSGKVLRPKATENVEAVVWVKVDSNAFTGSVARRL
HHHHHHHHCCCEEEEHHHHHHHHHHHCCCEECCCCCCCCEEEEEEEECCCCCHHHHHHHH
SNIEGVKEVSEVTGDYDLLVRIEAPDHKVLNTILDTVRGMKGIVGTHTQIVLKKLEK
HHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479 [H]