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The map label for this gene is leuD [H]

Identifier: 156938035

GI number: 156938035

Start: 1108024

End: 1108551

Strand: Reverse

Name: leuD [H]

Synonym: Igni_1248

Alternate gene names: 156938035

Gene position: 1108551-1108024 (Counterclockwise)

Preceding gene: 156938037

Following gene: 156938034

Centisome position: 85.43

GC content: 58.52

Gene sequence:

>528_bases
GTGACCGGAGCTATAAGGGCGAAGGCCATTAAGGTGGGCGACAACATAGACACGGACGTCATAATTCCGGGGCGTTACTT
GATCTATACTGACCCCGAAACTCTGGGCAAGCACTGCTTCGAGCCGATAATGCCGGACTTCTACCAGCGCAGCAAGGAGC
TGGGCGGCGTCATAGTCGTCGCGGGCAAGAACTTCGGGATGGGGAGCTCCAGGGAGCAAGCCGTTGTGGCCTTAAAGGGC
GCCGGGGTTAAGGCGGTGGTCGCGGAGAGCTTCGCCAGAATATTCTATAGGAACTGCATAAACCAAGGCTTGCCCGCGGT
GGCCGTCAAGGGGATAAGCGAGGCGGTGGAAGAGGGGGACGAGGTCGAGGTAGACCTAGAGGGAGGGAAGGTGAGGGTTT
ACGACAAGGAAGGGAACCTCAAGAAGGAGTTCGACGTCCCCAAGCTCCCCCCGAGCTTACTGGAGATCTTGGAGAGCGGG
GGCTTGGTCCCCTACCTTAAGAAGAGGTTGGCGGTGGAGGGCAAATGA

Upstream 100 bases:

>100_bases
TCACCAAAGCCGACTACCGGACGCGCGCTTAGTAGGAAATAATAGGGTTTTTGCAGCAACACTATATAACCTCCTCCCCG
AGTCCGACCGGGATCGCGCA

Downstream 100 bases:

>100_bases
GGGCCGTCAAGTTCCGAGGGGACTTGGTCGTCGAAGAGACGCCCATTCCCCCCGTCCCGAAGGACCACGTCCTCGTAAAG
GTGAAGTTAGCCTCGGTCTC

Product: 3-isopropylmalate dehydratase, small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 175; Mature: 174

Protein sequence:

>175_residues
MTGAIRAKAIKVGDNIDTDVIIPGRYLIYTDPETLGKHCFEPIMPDFYQRSKELGGVIVVAGKNFGMGSSREQAVVALKG
AGVKAVVAESFARIFYRNCINQGLPAVAVKGISEAVEEGDEVEVDLEGGKVRVYDKEGNLKKEFDVPKLPPSLLEILESG
GLVPYLKKRLAVEGK

Sequences:

>Translated_175_residues
MTGAIRAKAIKVGDNIDTDVIIPGRYLIYTDPETLGKHCFEPIMPDFYQRSKELGGVIVVAGKNFGMGSSREQAVVALKG
AGVKAVVAESFARIFYRNCINQGLPAVAVKGISEAVEEGDEVEVDLEGGKVRVYDKEGNLKKEFDVPKLPPSLLEILESG
GLVPYLKKRLAVEGK
>Mature_174_residues
TGAIRAKAIKVGDNIDTDVIIPGRYLIYTDPETLGKHCFEPIMPDFYQRSKELGGVIVVAGKNFGMGSSREQAVVALKGA
GVKAVVAESFARIFYRNCINQGLPAVAVKGISEAVEEGDEVEVDLEGGKVRVYDKEGNLKKEFDVPKLPPSLLEILESGG
LVPYLKKRLAVEGK

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=142, Percent_Identity=36.6197183098592, Blast_Score=70, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6320440, Length=175, Percent_Identity=36, Blast_Score=82, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6321429, Length=145, Percent_Identity=31.7241379310345, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011827 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 18923; Mature: 18792

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGAIRAKAIKVGDNIDTDVIIPGRYLIYTDPETLGKHCFEPIMPDFYQRSKELGGVIVV
CCCCCEEEEEEECCCCCCCEEECCEEEEEECHHHHHHHHHHHCCHHHHHHHHHCCCEEEE
AGKNFGMGSSREQAVVALKGAGVKAVVAESFARIFYRNCINQGLPAVAVKGISEAVEEGD
ECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
EVEVDLEGGKVRVYDKEGNLKKEFDVPKLPPSLLEILESGGLVPYLKKRLAVEGK
EEEEEECCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCCC
>Mature Secondary Structure 
TGAIRAKAIKVGDNIDTDVIIPGRYLIYTDPETLGKHCFEPIMPDFYQRSKELGGVIVV
CCCCEEEEEEECCCCCCCEEECCEEEEEECHHHHHHHHHHHCCHHHHHHHHHCCCEEEE
AGKNFGMGSSREQAVVALKGAGVKAVVAESFARIFYRNCINQGLPAVAVKGISEAVEEGD
ECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
EVEVDLEGGKVRVYDKEGNLKKEFDVPKLPPSLLEILESGGLVPYLKKRLAVEGK
EEEEEECCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA