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The map label for this gene is ygcB [C]

Identifier: 156937925

GI number: 156937925

Start: 1014625

End: 1016256

Strand: Reverse

Name: ygcB [C]

Synonym: Igni_1137

Alternate gene names: 156937925

Gene position: 1016256-1014625 (Counterclockwise)

Preceding gene: 156937926

Following gene: 156937924

Centisome position: 78.32

GC content: 58.21

Gene sequence:

>1632_bases
GTGCCTTTCAAGCCTAAGGCCGCTCACGAAGAGGCGGTAAGGCTTCTGGAGGAGAGCAACGTCGTCATGGTAGCCCCGAC
CAGCTACGGGAAGAGCAAGGGAGTACCCTACATGCAAGACCGGCTGAAGCTCCCGAGGAGCGTCCATTCCATGCCTTTGA
GGAGCTTGGTAATATCTCAATATAACTTCTTGCTCCGCGAGTACGGGGAGGCGTGTCACGCGTCCGGCCTTCAGCTGGAG
GGCAAGTGTCCCTACTTCGGGGGCAAGAGGGTAGTTTCGACGGTAGACTACTTGTCCTTGAACTTGTTGAGGCTGCCCCC
GGCGGAGCTCGGGTGGGCGTTGAAGTACAGCTACGGCCACTACGAGTACCCCAGAGCCAATTTGTTCTCTTCCTTGGTGG
TGTTCGACGAGGCCCACTTGCTCTCAGAACCTTGGAGCGGGGGCGAGAGCGCCGGGAGGGACTTCCTCCACGCCTCCTTG
GAGGCCCTCGGCGCCCTCTCCCAAGGCTTGAGGTTCAAGGTGTTAGTCGCCACCGCGACCTTGCCGGAGGTCGAGCTGAA
GAAGATAGTTAAGAAAATGAAGCCCAAGGCCGAGGTGGTCGCGGTCTGTAAGGGTTGTTACGGCGGCTTGAGAAACATAA
AGAGGGTAAACGACTTCTCCTCGGACGTCCGCTGGGAGACGGAGGCGGAGGGAGTCACGCTGCTGAACTACCTCCAAAGC
GAGTTGAAGAGGGTAGAGGACGAAGCCTCCTCTGGGAAGGTGCTGATAGTTGCCAACACCGTTAAGGAGGCCATCAAGGC
GGCCGAGCTCCTTAGGCCTTACTTCGGCAGCGACTTGGTGCTGGTCCACGGGAGGCTGAGCGAGGAGGATAAGAAAAATT
CTGTGAACAAGATAGAGAAAGCTAAGGTCGTGGTCAGCACCCAAGTGATAGAGGCCGGAGTCGACGTGGACGCCACTTGG
CTGATAACCGAGGCCGCGCCCGTCAGCTCCCTGGCCCAGAGGGCCGGGAGGCTCTGTAGGTCTAGGGACTGCGACGTAGC
TAAGGTAACGGTGCTCGCGCCCTCCAAGGGAGATCCTTACGGCGACGCCTCCGAGAAGGCCTTCAAGACGGTGAAGGAAG
TAGCGGAGGGAGGTGGGATAGAGTGGAGGGAACTGGACGACACGCAGAGCGGGAAGACCTTCTTGAGCTTGATAAACGCG
TTGGAGAGTAACTTCAAACCTTACCAAACTAAGAGCACGATTACTAAAATAATAACTACGCCTTTGCCGAACATAAAGGG
CATAAAAGAGCTGCTGGACGAGCTCTGCTCGTTCGTGAGGGACTCCGCGCTAATAGAGCTCAGGGCGGGCGAGGAGAGGC
TAGAGGTCTCCTTGAGCTGGCTCGCGGGAGCGCTCAAGAGGGGTTTGGAGGTGAGCGGCGTGAAGGCCGTTAAGTTCGGC
GGCGAGGAGCCCTTGAGCGAAGGGGACGTCAGCAACTTTCTCAAAAGGCTCGTCAAGGCTAAGAACAGCCCCAAGGCCTC
GTGTAGAGCCTACTCGACGCTCCTCGAGAAGTACGGCGCCTTGAGCTTGGCCGTGGAGGTCCCAGCGGACGAGTACAGAA
AGGGGTGGGGGATGAGAGTTGACTTGCGTTGA

Upstream 100 bases:

>100_bases
CTTGTACGACTGCGCCCGAAGGTGTGCTGAGGAGGAAGACGCGCGCGTCCGGGCGCTGGTCAATAAGATAAAGAAAATAA
CCAAAGAAGCGGTGGTCTAG

Downstream 100 bases:

>100_bases
CGCGCCGGCGTTCTACGAGGTGAGGGGAGGGAAGGAGGTCTTCGAGGGCCTCTGTGAGCACAGCCTTAGGACGGCTGCCG
CGGGCTTAGCGCTGTTCGAG

Product: CRISPR-associated helicase Cas3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 543; Mature: 542

Protein sequence:

>543_residues
MPFKPKAAHEEAVRLLEESNVVMVAPTSYGKSKGVPYMQDRLKLPRSVHSMPLRSLVISQYNFLLREYGEACHASGLQLE
GKCPYFGGKRVVSTVDYLSLNLLRLPPAELGWALKYSYGHYEYPRANLFSSLVVFDEAHLLSEPWSGGESAGRDFLHASL
EALGALSQGLRFKVLVATATLPEVELKKIVKKMKPKAEVVAVCKGCYGGLRNIKRVNDFSSDVRWETEAEGVTLLNYLQS
ELKRVEDEASSGKVLIVANTVKEAIKAAELLRPYFGSDLVLVHGRLSEEDKKNSVNKIEKAKVVVSTQVIEAGVDVDATW
LITEAAPVSSLAQRAGRLCRSRDCDVAKVTVLAPSKGDPYGDASEKAFKTVKEVAEGGGIEWRELDDTQSGKTFLSLINA
LESNFKPYQTKSTITKIITTPLPNIKGIKELLDELCSFVRDSALIELRAGEERLEVSLSWLAGALKRGLEVSGVKAVKFG
GEEPLSEGDVSNFLKRLVKAKNSPKASCRAYSTLLEKYGALSLAVEVPADEYRKGWGMRVDLR

Sequences:

>Translated_543_residues
MPFKPKAAHEEAVRLLEESNVVMVAPTSYGKSKGVPYMQDRLKLPRSVHSMPLRSLVISQYNFLLREYGEACHASGLQLE
GKCPYFGGKRVVSTVDYLSLNLLRLPPAELGWALKYSYGHYEYPRANLFSSLVVFDEAHLLSEPWSGGESAGRDFLHASL
EALGALSQGLRFKVLVATATLPEVELKKIVKKMKPKAEVVAVCKGCYGGLRNIKRVNDFSSDVRWETEAEGVTLLNYLQS
ELKRVEDEASSGKVLIVANTVKEAIKAAELLRPYFGSDLVLVHGRLSEEDKKNSVNKIEKAKVVVSTQVIEAGVDVDATW
LITEAAPVSSLAQRAGRLCRSRDCDVAKVTVLAPSKGDPYGDASEKAFKTVKEVAEGGGIEWRELDDTQSGKTFLSLINA
LESNFKPYQTKSTITKIITTPLPNIKGIKELLDELCSFVRDSALIELRAGEERLEVSLSWLAGALKRGLEVSGVKAVKFG
GEEPLSEGDVSNFLKRLVKAKNSPKASCRAYSTLLEKYGALSLAVEVPADEYRKGWGMRVDLR
>Mature_542_residues
PFKPKAAHEEAVRLLEESNVVMVAPTSYGKSKGVPYMQDRLKLPRSVHSMPLRSLVISQYNFLLREYGEACHASGLQLEG
KCPYFGGKRVVSTVDYLSLNLLRLPPAELGWALKYSYGHYEYPRANLFSSLVVFDEAHLLSEPWSGGESAGRDFLHASLE
ALGALSQGLRFKVLVATATLPEVELKKIVKKMKPKAEVVAVCKGCYGGLRNIKRVNDFSSDVRWETEAEGVTLLNYLQSE
LKRVEDEASSGKVLIVANTVKEAIKAAELLRPYFGSDLVLVHGRLSEEDKKNSVNKIEKAKVVVSTQVIEAGVDVDATWL
ITEAAPVSSLAQRAGRLCRSRDCDVAKVTVLAPSKGDPYGDASEKAFKTVKEVAEGGGIEWRELDDTQSGKTFLSLINAL
ESNFKPYQTKSTITKIITTPLPNIKGIKELLDELCSFVRDSALIELRAGEERLEVSLSWLAGALKRGLEVSGVKAVKFGG
EEPLSEGDVSNFLKRLVKAKNSPKASCRAYSTLLEKYGALSLAVEVPADEYRKGWGMRVDLR

Specific function: Unknown

COG id: COG1203

COG function: function code R; Predicted helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR006474
- InterPro:   IPR014021 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 59705; Mature: 59574

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFKPKAAHEEAVRLLEESNVVMVAPTSYGKSKGVPYMQDRLKLPRSVHSMPLRSLVISQ
CCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHH
YNFLLREYGEACHASGLQLEGKCPYFGGKRVVSTVDYLSLNLLRLPPAELGWALKYSYGH
HHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHEEECCCHHHCEEEEECCCC
YEYPRANLFSSLVVFDEAHLLSEPWSGGESAGRDFLHASLEALGALSQGLRFKVLVATAT
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECC
LPEVELKKIVKKMKPKAEVVAVCKGCYGGLRNIKRVNDFSSDVRWETEAEGVTLLNYLQS
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCCHHHHHHHHH
ELKRVEDEASSGKVLIVANTVKEAIKAAELLRPYFGSDLVLVHGRLSEEDKKNSVNKIEK
HHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHH
AKVVVSTQVIEAGVDVDATWLITEAAPVSSLAQRAGRLCRSRDCDVAKVTVLAPSKGDPY
HHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCC
GDASEKAFKTVKEVAEGGGIEWRELDDTQSGKTFLSLINALESNFKPYQTKSTITKIITT
CCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHC
PLPNIKGIKELLDELCSFVRDSALIELRAGEERLEVSLSWLAGALKRGLEVSGVKAVKFG
CCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEECC
GEEPLSEGDVSNFLKRLVKAKNSPKASCRAYSTLLEKYGALSLAVEVPADEYRKGWGMRV
CCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCHHHHHCCCCEEE
DLR
ECC
>Mature Secondary Structure 
PFKPKAAHEEAVRLLEESNVVMVAPTSYGKSKGVPYMQDRLKLPRSVHSMPLRSLVISQ
CCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHH
YNFLLREYGEACHASGLQLEGKCPYFGGKRVVSTVDYLSLNLLRLPPAELGWALKYSYGH
HHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHEEECCCHHHCEEEEECCCC
YEYPRANLFSSLVVFDEAHLLSEPWSGGESAGRDFLHASLEALGALSQGLRFKVLVATAT
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECC
LPEVELKKIVKKMKPKAEVVAVCKGCYGGLRNIKRVNDFSSDVRWETEAEGVTLLNYLQS
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCCHHHHHHHHH
ELKRVEDEASSGKVLIVANTVKEAIKAAELLRPYFGSDLVLVHGRLSEEDKKNSVNKIEK
HHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHH
AKVVVSTQVIEAGVDVDATWLITEAAPVSSLAQRAGRLCRSRDCDVAKVTVLAPSKGDPY
HHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCC
GDASEKAFKTVKEVAEGGGIEWRELDDTQSGKTFLSLINALESNFKPYQTKSTITKIITT
CCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHC
PLPNIKGIKELLDELCSFVRDSALIELRAGEERLEVSLSWLAGALKRGLEVSGVKAVKFG
CCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEECC
GEEPLSEGDVSNFLKRLVKAKNSPKASCRAYSTLLEKYGALSLAVEVPADEYRKGWGMRV
CCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCHHHHHCCCCEEE
DLR
ECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]