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The map label for this gene is leuB [H]

Identifier: 156937773

GI number: 156937773

Start: 874935

End: 875966

Strand: Direct

Name: leuB [H]

Synonym: Igni_0982

Alternate gene names: 156937773

Gene position: 874935-875966 (Clockwise)

Preceding gene: 156937772

Following gene: 156937775

Centisome position: 67.43

GC content: 59.21

Gene sequence:

>1032_bases
ATGGTAACCGTTGCCGTCATAGAGGGCGACGGTATAGGCCCCGAGGTCGTGGGCGCGACCCTTAAGGTGCTCGAAAAGAT
AAGGGAAACTTTCAAACTGCCGCTGGAGTTCGTCTTCGTGGAGGCGGGCGACAGAGCTAAGGAGAAGTACGGCGAGGCCC
TCCCTAAGGAGAGCTACGAGAGGCTCCTACGAGCGGACGCTATACTCAAGGGCCCCGTCGGCGAGACGGCAGCGGACGTC
ATAGTTAGGTTGAGGAGGGAGCTGGACCTCTTCGCCAACATAAGGCCCGCGAAGGTCTTGCCCGGAGTCCCCGCACTTAA
GGAAAATGTAGATCTAATTATAGTTAGGGAAAATATAGAGGACCTTTACGTTGGTGCCGAGAACCTCTTGCCCCAGACCT
CCTTGGGCCACAAGGTCGCGGTGGGCCTCAGACTGGCTTCAGAGAGGGAGACGAGGAGGGTGGCGAAGGTCGCCGCGGAG
TACGCTAAAGCCCGTAGGAACAAAGTAACTATAGTACACAAGGCTAACGTAATGAGGGTGACGTGCGGGCTCTTCAGAGA
CGTCGCGAAGGAGGTCTTGGAGGCTGAGGGGGTTGAGGTAGACGAGATGTACGTGGACGCGGCCGCTATGGAGTTGGTCA
GAAGGCCGGAGAGGTTCGACGTAATGCTGACTCCGAACGTCTTCGGGGACATACTCTCCGACTTGGCGGCCCAAGTGGTC
GGCAGCTTGGGCTTGGCGCCCAGCGGCAACATAGGCGAGGAGCGCGCGCTGTTCGAGCCGGTCCACGGGGCGGCCTTCGA
CATAGCGGGTAAGGGGATAGCCAACCCTACGGCGACCATGCTAGCGGCGTCGATGATGTTGGAGTGGTTAGAGAGGAAGA
AGGGGCTCCCCAAAGGGGCGGAAGCGGCCGCTGCGCTTCAGAGGGCGATAGAGAAGGCCTTGTCAGAGGGAGTAAAGACG
CCGGACTTGGGCGGGAACTACGGCACCTTCGAGTTCGCAGAAGAGGTCGTTAAGAGGTTGCAGCTTCCTTAG

Upstream 100 bases:

>100_bases
GGGGGCCTACGTGGCGTCCATCACGGTGGGCTTCAAGCTGTTGATTGTCCCCACGAAATGATTTTTGTCCCTACTCCTCC
TACACTCCCGGTAGGTGTTT

Downstream 100 bases:

>100_bases
CCCTCTCCCTCTCCTCTAAGACCTTTTCTACGATCTCCCTCAGCTTGTCCTCGGTAACCCTAATGCCCATGTCGCCGAGC
CTCTTAACCTCGTCTAACAC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; IMDH; Beta-IPM dehydrogenase [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MVTVAVIEGDGIGPEVVGATLKVLEKIRETFKLPLEFVFVEAGDRAKEKYGEALPKESYERLLRADAILKGPVGETAADV
IVRLRRELDLFANIRPAKVLPGVPALKENVDLIIVRENIEDLYVGAENLLPQTSLGHKVAVGLRLASERETRRVAKVAAE
YAKARRNKVTIVHKANVMRVTCGLFRDVAKEVLEAEGVEVDEMYVDAAAMELVRRPERFDVMLTPNVFGDILSDLAAQVV
GSLGLAPSGNIGEERALFEPVHGAAFDIAGKGIANPTATMLAASMMLEWLERKKGLPKGAEAAAALQRAIEKALSEGVKT
PDLGGNYGTFEFAEEVVKRLQLP

Sequences:

>Translated_343_residues
MVTVAVIEGDGIGPEVVGATLKVLEKIRETFKLPLEFVFVEAGDRAKEKYGEALPKESYERLLRADAILKGPVGETAADV
IVRLRRELDLFANIRPAKVLPGVPALKENVDLIIVRENIEDLYVGAENLLPQTSLGHKVAVGLRLASERETRRVAKVAAE
YAKARRNKVTIVHKANVMRVTCGLFRDVAKEVLEAEGVEVDEMYVDAAAMELVRRPERFDVMLTPNVFGDILSDLAAQVV
GSLGLAPSGNIGEERALFEPVHGAAFDIAGKGIANPTATMLAASMMLEWLERKKGLPKGAEAAAALQRAIEKALSEGVKT
PDLGGNYGTFEFAEEVVKRLQLP
>Mature_343_residues
MVTVAVIEGDGIGPEVVGATLKVLEKIRETFKLPLEFVFVEAGDRAKEKYGEALPKESYERLLRADAILKGPVGETAADV
IVRLRRELDLFANIRPAKVLPGVPALKENVDLIIVRENIEDLYVGAENLLPQTSLGHKVAVGLRLASERETRRVAKVAAE
YAKARRNKVTIVHKANVMRVTCGLFRDVAKEVLEAEGVEVDEMYVDAAAMELVRRPERFDVMLTPNVFGDILSDLAAQVV
GSLGLAPSGNIGEERALFEPVHGAAFDIAGKGIANPTATMLAASMMLEWLERKKGLPKGAEAAAALQRAIEKALSEGVKT
PDLGGNYGTFEFAEEVVKRLQLP

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=349, Percent_Identity=37.8223495702006, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI4758582, Length=356, Percent_Identity=34.2696629213483, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI28178821, Length=356, Percent_Identity=35.1123595505618, Blast_Score=170, Evalue=1e-42,
Organism=Homo sapiens, GI28178816, Length=358, Percent_Identity=34.9162011173184, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI28178838, Length=336, Percent_Identity=34.8214285714286, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI28178819, Length=218, Percent_Identity=36.697247706422, Blast_Score=121, Evalue=7e-28,
Organism=Escherichia coli, GI87081683, Length=344, Percent_Identity=36.6279069767442, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1788101, Length=362, Percent_Identity=35.9116022099448, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1787381, Length=355, Percent_Identity=32.6760563380282, Blast_Score=161, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI71986051, Length=348, Percent_Identity=37.9310344827586, Blast_Score=210, Evalue=7e-55,
Organism=Caenorhabditis elegans, GI25144293, Length=347, Percent_Identity=36.0230547550432, Blast_Score=178, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI17550882, Length=359, Percent_Identity=33.983286908078, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17505779, Length=355, Percent_Identity=32.6760563380282, Blast_Score=161, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6322097, Length=357, Percent_Identity=40.6162464985994, Blast_Score=241, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6324709, Length=351, Percent_Identity=36.7521367521368, Blast_Score=193, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6324291, Length=349, Percent_Identity=33.810888252149, Blast_Score=183, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6319830, Length=368, Percent_Identity=31.25, Blast_Score=145, Evalue=7e-36,
Organism=Drosophila melanogaster, GI24643270, Length=349, Percent_Identity=39.2550143266476, Blast_Score=220, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24643268, Length=349, Percent_Identity=39.2550143266476, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24661184, Length=351, Percent_Identity=36.1823361823362, Blast_Score=207, Evalue=7e-54,
Organism=Drosophila melanogaster, GI161078635, Length=351, Percent_Identity=31.3390313390313, Blast_Score=160, Evalue=8e-40,
Organism=Drosophila melanogaster, GI161078637, Length=351, Percent_Identity=31.3390313390313, Blast_Score=160, Evalue=9e-40,
Organism=Drosophila melanogaster, GI161078633, Length=351, Percent_Identity=31.3390313390313, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24650122, Length=351, Percent_Identity=31.3390313390313, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI161078639, Length=349, Percent_Identity=31.2320916905444, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI281362242, Length=351, Percent_Identity=32.7635327635328, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24648872, Length=351, Percent_Identity=32.7635327635328, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI20130355, Length=342, Percent_Identity=27.4853801169591, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 37218; Mature: 37218

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTVAVIEGDGIGPEVVGATLKVLEKIRETFKLPLEFVFVEAGDRAKEKYGEALPKESYE
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHCCCHHHHH
RLLRADAILKGPVGETAADVIVRLRRELDLFANIRPAKVLPGVPALKENVDLIIVRENIE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHCCCCEEEEECCCH
DLYVGAENLLPQTSLGHKVAVGLRLASERETRRVAKVAAEYAKARRNKVTIVHKANVMRV
HHHHCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHH
TCGLFRDVAKEVLEAEGVEVDEMYVDAAAMELVRRPERFDVMLTPNVFGDILSDLAAQVV
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHH
GSLGLAPSGNIGEERALFEPVHGAAFDIAGKGIANPTATMLAASMMLEWLERKKGLPKGA
HHCCCCCCCCCCCHHHHHCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCH
EAAAALQRAIEKALSEGVKTPDLGGNYGTFEFAEEVVKRLQLP
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MVTVAVIEGDGIGPEVVGATLKVLEKIRETFKLPLEFVFVEAGDRAKEKYGEALPKESYE
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHCCCHHHHH
RLLRADAILKGPVGETAADVIVRLRRELDLFANIRPAKVLPGVPALKENVDLIIVRENIE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHCCCCEEEEECCCH
DLYVGAENLLPQTSLGHKVAVGLRLASERETRRVAKVAAEYAKARRNKVTIVHKANVMRV
HHHHCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHH
TCGLFRDVAKEVLEAEGVEVDEMYVDAAAMELVRRPERFDVMLTPNVFGDILSDLAAQVV
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHH
GSLGLAPSGNIGEERALFEPVHGAAFDIAGKGIANPTATMLAASMMLEWLERKKGLPKGA
HHCCCCCCCCCCCHHHHHCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCH
EAAAALQRAIEKALSEGVKTPDLGGNYGTFEFAEEVVKRLQLP
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9023199; 11572479; 7557336 [H]