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The map label for this gene is ilvD

Identifier: 156937752

GI number: 156937752

Start: 851968

End: 853626

Strand: Reverse

Name: ilvD

Synonym: Igni_0961

Alternate gene names: 156937752

Gene position: 853626-851968 (Counterclockwise)

Preceding gene: 156937759

Following gene: 156937751

Centisome position: 65.79

GC content: 58.53

Gene sequence:

>1659_bases
TTGAAGAGAAGCGACGTCTTCTTGAGGCCGGAGTACGCCCCTCATAGGGCTCTGTGGAGGGCCTCTGGGCTAATTGACGA
AGAGCTGAGGAGGCCTCTAATAGGAGTGGCGAACTCTTGGAACGAAATAGTCCCCGGCCACGTCCACTTGGACAAGGTAG
CGGAGGCGGTAAAGGCCGGGATAAGGATGGCAGGGGGCACTCCGCTGGAGTTCGGCACCATCGCCGTATGCGACGGGATC
GCAATGGGTCACGAGGGGATGAGGTATTCGCTGCCTTCTAGGGAGGTCATAGCAGACACTGTCGAAATAATGGTGGAAGC
TCACAGGCTGGACGCCGTAGTTATGGTGACCAACTGCGACAAGATAACTCCGGGCTTCTTGTTGGCCGCGGCCAGGCTGG
AGGTCCCGGTAATTCTGATCAACGGCGGCCCCATGATGCCCGGCGTATACGGCAAGGAACGGATAGACTTCAAGGACCTC
ATGGAGAGGATGAACGTCCTCATTAAGGAGGGAAGAACGGAAGAACTAAGGAAGTTAGAGGAGAGCGCGCTCCCCGGCCC
GGGCAGCTGCGCGGGCCTCTTCACGGCAAACACGATGAATATGCTCTCGGAAGCCATGGGGTTGATGTTGCCGGGCGCGT
CCACGGTACCAGCAGTGGAGGCGAGGAGGCTCTGGTACGCCAAGCTTACCGGTATGAGAATAGTAAAAATGGTGGAAGAG
GGACTGACTCCCGACAAAATACTGACAAGGAAGGCATTAGAGAACGCTATAGCCGTGGACATGGCGTTGGGCGGCTCTAC
CAACTCCGTGCTACACTTGGAAGCGTTAGCGTACGAGCTCGGAATAGACTTACCCCTCGAAGTGTTCGACGAAATATCCC
GCAAGGTGCCCCACATAGCTTCCATATCGCCTTCCGGGAGACACTTCGTAGTGGACTTGGACAGAGCCGGGGGGATTCCG
GCAGTACTAAAAGAGCTCGGGGAGGCCGGTCTCATTCACAAGGACGCCTTGACCGTGACCGGCAAGACCGTCTGGGAGAA
CGTTAAGGACGCGGCAGTCTTGGATAGAGAAGTAATAAGGCCTTTGGATAACCCCTACAGTCCCTTCGGAGGCCTTGCCA
TACTCAAGGGCTCCTTGGCCCCTAACGGAGCCGTGGTTAAGGCTTCAGCCGTGAAGAGGGAGCTCTGGAAGTTCAAGGGC
GTGGCGAGGGTCTTTGACAGAGAGGAGGACGCGGTAAAGGCCATCAGAGGGGGCGAGATAGAGCCCGGGACCGTAATAGT
GATCAGGTACGAGGGCCCGAGAGGGGGGCCCGGGATGCGCGAGATGTTGACGGCCACTGCAGCAGTTATGGCGCTCGGCT
TGGGCGATAAGGTAGCGCTGGTGACGGACGGCCGCTTCTCGGGGGCCACTAGGGGGCCAGCGATAGGCCACGTCTCCCCC
GAGGCCGCCGCCGGAGGGCCCATAGCGCTCGTTCAAGACGGCGACGAGATAGTGATAGATATCGAGAAGAGGAGGTTAGA
CTTGCTGGTGGACGAGAAGGAGCTAGAGGAGAGGAGGGCTCGCTGGAAGCCGAAGGTGAAGCCCTTGAGGAGGGGAATAC
TTAGGAGGTACGCGAAGATGGCCTTGAGCGCCGACAAGGGAGGGGCGTTAGAGTACTGA

Upstream 100 bases:

>100_bases
AAGTCGCTACCCGGTGTGGAAAACGCAGCTGGGTTTTTGACATTAAGCGCGCTACGAACGAGTTTTAGCTTCCGCGTGGG
AGTGGAAGTGGTCCCGGAAG

Downstream 100 bases:

>100_bases
ACAGTTAGCTATTGTTAGAGTACGTCCGTTGGGGGTTTATGCGAGAGGGAAGGGGAGTGAAGACGGTAACGCGTTCGATC
AAAACGTTCTAAGCAAGAAG

Product: dihydroxyacid dehydratase

Products: NA

Alternate protein names: DAD

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MKRSDVFLRPEYAPHRALWRASGLIDEELRRPLIGVANSWNEIVPGHVHLDKVAEAVKAGIRMAGGTPLEFGTIAVCDGI
AMGHEGMRYSLPSREVIADTVEIMVEAHRLDAVVMVTNCDKITPGFLLAAARLEVPVILINGGPMMPGVYGKERIDFKDL
MERMNVLIKEGRTEELRKLEESALPGPGSCAGLFTANTMNMLSEAMGLMLPGASTVPAVEARRLWYAKLTGMRIVKMVEE
GLTPDKILTRKALENAIAVDMALGGSTNSVLHLEALAYELGIDLPLEVFDEISRKVPHIASISPSGRHFVVDLDRAGGIP
AVLKELGEAGLIHKDALTVTGKTVWENVKDAAVLDREVIRPLDNPYSPFGGLAILKGSLAPNGAVVKASAVKRELWKFKG
VARVFDREEDAVKAIRGGEIEPGTVIVIRYEGPRGGPGMREMLTATAAVMALGLGDKVALVTDGRFSGATRGPAIGHVSP
EAAAGGPIALVQDGDEIVIDIEKRRLDLLVDEKELEERRARWKPKVKPLRRGILRRYAKMALSADKGGALEY

Sequences:

>Translated_552_residues
MKRSDVFLRPEYAPHRALWRASGLIDEELRRPLIGVANSWNEIVPGHVHLDKVAEAVKAGIRMAGGTPLEFGTIAVCDGI
AMGHEGMRYSLPSREVIADTVEIMVEAHRLDAVVMVTNCDKITPGFLLAAARLEVPVILINGGPMMPGVYGKERIDFKDL
MERMNVLIKEGRTEELRKLEESALPGPGSCAGLFTANTMNMLSEAMGLMLPGASTVPAVEARRLWYAKLTGMRIVKMVEE
GLTPDKILTRKALENAIAVDMALGGSTNSVLHLEALAYELGIDLPLEVFDEISRKVPHIASISPSGRHFVVDLDRAGGIP
AVLKELGEAGLIHKDALTVTGKTVWENVKDAAVLDREVIRPLDNPYSPFGGLAILKGSLAPNGAVVKASAVKRELWKFKG
VARVFDREEDAVKAIRGGEIEPGTVIVIRYEGPRGGPGMREMLTATAAVMALGLGDKVALVTDGRFSGATRGPAIGHVSP
EAAAGGPIALVQDGDEIVIDIEKRRLDLLVDEKELEERRARWKPKVKPLRRGILRRYAKMALSADKGGALEY
>Mature_552_residues
MKRSDVFLRPEYAPHRALWRASGLIDEELRRPLIGVANSWNEIVPGHVHLDKVAEAVKAGIRMAGGTPLEFGTIAVCDGI
AMGHEGMRYSLPSREVIADTVEIMVEAHRLDAVVMVTNCDKITPGFLLAAARLEVPVILINGGPMMPGVYGKERIDFKDL
MERMNVLIKEGRTEELRKLEESALPGPGSCAGLFTANTMNMLSEAMGLMLPGASTVPAVEARRLWYAKLTGMRIVKMVEE
GLTPDKILTRKALENAIAVDMALGGSTNSVLHLEALAYELGIDLPLEVFDEISRKVPHIASISPSGRHFVVDLDRAGGIP
AVLKELGEAGLIHKDALTVTGKTVWENVKDAAVLDREVIRPLDNPYSPFGGLAILKGSLAPNGAVVKASAVKRELWKFKG
VARVFDREEDAVKAIRGGEIEPGTVIVIRYEGPRGGPGMREMLTATAAVMALGLGDKVALVTDGRFSGATRGPAIGHVSP
EAAAGGPIALVQDGDEIVIDIEKRRLDLLVDEKELEERRARWKPKVKPLRRGILRRYAKMALSADKGGALEY

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family

Homologues:

Organism=Escherichia coli, GI48994964, Length=591, Percent_Identity=44.839255499154, Blast_Score=467, Evalue=1e-133,
Organism=Escherichia coli, GI1788157, Length=522, Percent_Identity=32.7586206896552, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI1786464, Length=492, Percent_Identity=33.9430894308943, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI2367371, Length=494, Percent_Identity=32.1862348178138, Blast_Score=225, Evalue=5e-60,
Organism=Saccharomyces cerevisiae, GI6322476, Length=562, Percent_Identity=43.0604982206406, Blast_Score=435, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ILVD_IGNH4 (A8AB39)

Other databases:

- EMBL:   CP000816
- RefSeq:   YP_001435548.1
- ProteinModelPortal:   A8AB39
- SMR:   A8AB39
- STRING:   A8AB39
- GeneID:   5562278
- GenomeReviews:   CP000816_GR
- KEGG:   iho:Igni_0961
- eggNOG:   arNOG04340
- HOGENOM:   HBG671001
- OMA:   KVPCLSK
- BioCyc:   IHOS453591:IGNI_0961-MONOMER
- HAMAP:   MF_00012
- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558
- PANTHER:   PTHR21000
- TIGRFAMs:   TIGR00110

Pfam domain/function: PF00920 ILVD_EDD; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.9

Molecular weight: Translated: 59728; Mature: 59728

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00886 ILVD_EDD_1; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRSDVFLRPEYAPHRALWRASGLIDEELRRPLIGVANSWNEIVPGHVHLDKVAEAVKAG
CCCCCEEECCCCCCHHHHHHHCCCCHHHHCCCHHHHCCCCCCCCCCCEEHHHHHHHHHHH
IRMAGGTPLEFGTIAVCDGIAMGHEGMRYSLPSREVIADTVEIMVEAHRLDAVVMVTNCD
HEECCCCCCCCCCEEEECCHHCCCCCCEECCCCHHHHHHHHHHHHHHHHCCEEEEEECCC
KITPGFLLAAARLEVPVILINGGPMMPGVYGKERIDFKDLMERMNVLIKEGRTEELRKLE
CCCCHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHH
ESALPGPGSCAGLFTANTMNMLSEAMGLMLPGASTVPAVEARRLWYAKLTGMRIVKMVEE
HHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GLTPDKILTRKALENAIAVDMALGGSTNSVLHLEALAYELGIDLPLEVFDEISRKVPHIA
CCCHHHHHHHHHHHHHHEEEEEECCCCCCEEEHHHHHHHHCCCCCHHHHHHHHHCCCCEE
SISPSGRHFVVDLDRAGGIPAVLKELGEAGLIHKDALTVTGKTVWENVKDAAVLDREVIR
ECCCCCCEEEEEECCCCCCHHHHHHHCCCCCEECCCEEECCHHHHHHHHHHHHHHHHHHH
PLDNPYSPFGGLAILKGSLAPNGAVVKASAVKRELWKFKGVARVFDREEDAVKAIRGGEI
HCCCCCCCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCC
EPGTVIVIRYEGPRGGPGMREMLTATAAVMALGLGDKVALVTDGRFSGATRGPAIGHVSP
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCC
EAAAGGPIALVQDGDEIVIDIEKRRLDLLVDEKELEERRARWKPKVKPLRRGILRRYAKM
CCCCCCCEEEEECCCEEEEEEECCCEEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ALSADKGGALEY
HHCCCCCCCCCC
>Mature Secondary Structure
MKRSDVFLRPEYAPHRALWRASGLIDEELRRPLIGVANSWNEIVPGHVHLDKVAEAVKAG
CCCCCEEECCCCCCHHHHHHHCCCCHHHHCCCHHHHCCCCCCCCCCCEEHHHHHHHHHHH
IRMAGGTPLEFGTIAVCDGIAMGHEGMRYSLPSREVIADTVEIMVEAHRLDAVVMVTNCD
HEECCCCCCCCCCEEEECCHHCCCCCCEECCCCHHHHHHHHHHHHHHHHCCEEEEEECCC
KITPGFLLAAARLEVPVILINGGPMMPGVYGKERIDFKDLMERMNVLIKEGRTEELRKLE
CCCCHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHH
ESALPGPGSCAGLFTANTMNMLSEAMGLMLPGASTVPAVEARRLWYAKLTGMRIVKMVEE
HHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GLTPDKILTRKALENAIAVDMALGGSTNSVLHLEALAYELGIDLPLEVFDEISRKVPHIA
CCCHHHHHHHHHHHHHHEEEEEECCCCCCEEEHHHHHHHHCCCCCHHHHHHHHHCCCCEE
SISPSGRHFVVDLDRAGGIPAVLKELGEAGLIHKDALTVTGKTVWENVKDAAVLDREVIR
ECCCCCCEEEEEECCCCCCHHHHHHHCCCCCEECCCEEECCHHHHHHHHHHHHHHHHHHH
PLDNPYSPFGGLAILKGSLAPNGAVVKASAVKRELWKFKGVARVFDREEDAVKAIRGGEI
HCCCCCCCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCC
EPGTVIVIRYEGPRGGPGMREMLTATAAVMALGLGDKVALVTDGRFSGATRGPAIGHVSP
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCC
EAAAGGPIALVQDGDEIVIDIEKRRLDLLVDEKELEERRARWKPKVKPLRRGILRRYAKM
CCCCCCCEEEEECCCEEEEEEECCCEEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ALSADKGGALEY
HHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA