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The map label for this gene is thiD [C]

Identifier: 156937681

GI number: 156937681

Start: 787111

End: 788403

Strand: Direct

Name: thiD [C]

Synonym: Igni_0888

Alternate gene names: 156937681

Gene position: 787111-788403 (Clockwise)

Preceding gene: 156937679

Following gene: 156937682

Centisome position: 60.66

GC content: 59.55

Gene sequence:

>1293_bases
TTGAATGTGAAGAAGGTGGCGGCCGTCGGCGGACTCGACCCCTCGGGGGGCGCCGGGATAGAGATGGACGTCAAGGTCGG
CAGCGCCGTAGGCGTTCACGTTCACCCAGTGGCGACGGCCATCACTTACCAAACGCCCACCGAGTTCTACGGCTCTAAGT
GCACCGATATAGAGGTTCTGGAAGGCCAACTCAAGGCCGTAAAGGGAGTGCGGAACTGGAAGCTAGGGATGCTCTGTAAC
GAAAGGATCGCGGAGACCTTGTTGAAGTACTTAGAAGGGGTGGTTGTGGTAGATCCCGTACTCAAGGCCACGGCCGGAGG
AACGCTCTACGAGGGCAGTCTGGAAACTTACAAGAAGATCTTGTCGGTCTCCTTCGCAGTCACCCCCAACGCGCTCGAGG
CCGAGGCCCTCACGGGCTTGAGGGTCCGCACGGTGGATGACGCCATAAGGGCGGCAGAGGCCTTGGCAGAGGCGGGCCCT
AAACTCGTGGTAATAACTGGAGGTCACTTGGAGATCTTCGCCGACGTGATATATTACGACGGGTACGTAGAGGTGATAAG
GGGGACGAGGAAGGTAACCTCCTTCCACGGGTCGGGGAGCTTCATGGCCATGGCATTGGCTTCCTACTTGGCGTTGGGGG
AGGCCCCGGTGGTCGCCGCGAGGAAGGCCGTGGGCTACACTAGGGTCGCGCACCTCTTCTCCCAAGGCAAGGAGGCCCTC
GATCCCCTGTACCAAATGAGGTACGACGCGGTCAGACACTGTATGTACGAGGAGTACAAGTACTTCATAGAGTGGCTTGA
AAGCTTGCCCTTTGAGAAGGCAAAGAAGATCGCGCCGGAGGTAGGGATAAACGTCGCCTACTCGGTCCCGAAGGAGTTGG
TGAGGGGCAAGGGCTCGGTGCTAGGGGTCCCCGGGAGGCTACGGCTGACCCCCAGAGGCCTTAGGTCTTGTTGCAACCCT
TGGTGGGGCCAAGCCGACCACACGGCGCGCTTGCTGCTGGAGGCCCAGAAGTACAACCCGAAGCTGAGGGTCGCCATGAA
CATAAGGTACAGCCCGGAGAACGTCGCCGCGCTCTCCGGCGCGGGCTACGAGGTCTACGAGCTCAAGAGGGAGACCGAGC
CGCCGGGGGAGGAGAGTATGAGGTGGGCAGCGAAGAAGGCCTACTCCGACTTGGGCAAGGTGCCGGACGTCATATACGAC
AAGGGCTTCTACGGCAAGGAAGCCATGATAAGGTTGCTCACTGAGGGCTTAGAGGAGCTTAAGGAAATGTTGAGGGTAGT
CCTCCAGATTTAA

Upstream 100 bases:

>100_bases
CCGAAGGTCTCTCTTAGGACAGCTATGTAGGGACCCCATTCGACCTTCGAACACTTAATGTTGAACTTTTTCAACAGCAC
CTTATCATCACCCTAGGCGT

Downstream 100 bases:

>100_bases
GGAGCCTTTGGGGAGACGCGGCGGCGAAGGCCTTGAAGCAGCTCGCCGCTCTGCTGATCCTGACCTCCTTGGCCTTCGGA
CTGAGCCAGTGCTACTCTCA

Product: phosphomethylpyrimidine kinase type-1

Products: ADP; 4-amino-2-methyl-5-diphosphomethylpyrimidine

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MNVKKVAAVGGLDPSGGAGIEMDVKVGSAVGVHVHPVATAITYQTPTEFYGSKCTDIEVLEGQLKAVKGVRNWKLGMLCN
ERIAETLLKYLEGVVVVDPVLKATAGGTLYEGSLETYKKILSVSFAVTPNALEAEALTGLRVRTVDDAIRAAEALAEAGP
KLVVITGGHLEIFADVIYYDGYVEVIRGTRKVTSFHGSGSFMAMALASYLALGEAPVVAARKAVGYTRVAHLFSQGKEAL
DPLYQMRYDAVRHCMYEEYKYFIEWLESLPFEKAKKIAPEVGINVAYSVPKELVRGKGSVLGVPGRLRLTPRGLRSCCNP
WWGQADHTARLLLEAQKYNPKLRVAMNIRYSPENVAALSGAGYEVYELKRETEPPGEESMRWAAKKAYSDLGKVPDVIYD
KGFYGKEAMIRLLTEGLEELKEMLRVVLQI

Sequences:

>Translated_430_residues
MNVKKVAAVGGLDPSGGAGIEMDVKVGSAVGVHVHPVATAITYQTPTEFYGSKCTDIEVLEGQLKAVKGVRNWKLGMLCN
ERIAETLLKYLEGVVVVDPVLKATAGGTLYEGSLETYKKILSVSFAVTPNALEAEALTGLRVRTVDDAIRAAEALAEAGP
KLVVITGGHLEIFADVIYYDGYVEVIRGTRKVTSFHGSGSFMAMALASYLALGEAPVVAARKAVGYTRVAHLFSQGKEAL
DPLYQMRYDAVRHCMYEEYKYFIEWLESLPFEKAKKIAPEVGINVAYSVPKELVRGKGSVLGVPGRLRLTPRGLRSCCNP
WWGQADHTARLLLEAQKYNPKLRVAMNIRYSPENVAALSGAGYEVYELKRETEPPGEESMRWAAKKAYSDLGKVPDVIYD
KGFYGKEAMIRLLTEGLEELKEMLRVVLQI
>Mature_430_residues
MNVKKVAAVGGLDPSGGAGIEMDVKVGSAVGVHVHPVATAITYQTPTEFYGSKCTDIEVLEGQLKAVKGVRNWKLGMLCN
ERIAETLLKYLEGVVVVDPVLKATAGGTLYEGSLETYKKILSVSFAVTPNALEAEALTGLRVRTVDDAIRAAEALAEAGP
KLVVITGGHLEIFADVIYYDGYVEVIRGTRKVTSFHGSGSFMAMALASYLALGEAPVVAARKAVGYTRVAHLFSQGKEAL
DPLYQMRYDAVRHCMYEEYKYFIEWLESLPFEKAKKIAPEVGINVAYSVPKELVRGKGSVLGVPGRLRLTPRGLRSCCNP
WWGQADHTARLLLEAQKYNPKLRVAMNIRYSPENVAALSGAGYEVYELKRETEPPGEESMRWAAKKAYSDLGKVPDVIYD
KGFYGKEAMIRLLTEGLEELKEMLRVVLQI

Specific function: Catalyzes The Phosphorylation Of Hmp-P To Hmp-Pp. [C]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788420, Length=221, Percent_Identity=30.7692307692308, Blast_Score=68, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324517, Length=236, Percent_Identity=31.3559322033898, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324997, Length=236, Percent_Identity=29.6610169491525, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303
- InterPro:   IPR013749
- InterPro:   IPR019293 [H]

Pfam domain/function: PF10120 MethylPyrKinase; PF08543 Phos_pyr_kin [H]

EC number: 2.7.4.7

Molecular weight: Translated: 47133; Mature: 47133

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVKKVAAVGGLDPSGGAGIEMDVKVGSAVGVHVHPVATAITYQTPTEFYGSKCTDIEVL
CCCHHEEHCCCCCCCCCCCEEEEEEECCEECEEEECCEEEEEECCCHHHHCCCCCCHHHH
EGQLKAVKGVRNWKLGMLCNERIAETLLKYLEGVVVVDPVLKATAGGTLYEGSLETYKKI
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCEEEECHHHHHCCCCCEEECCHHHHHHH
LSVSFAVTPNALEAEALTGLRVRTVDDAIRAAEALAEAGPKLVVITGGHLEIFADVIYYD
HHHHEEECCCCCCHHHHCCCEEEHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHC
GYVEVIRGTRKVTSFHGSGSFMAMALASYLALGEAPVVAARKAVGYTRVAHLFSQGKEAL
CHHHHHHCCHHHEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHH
DPLYQMRYDAVRHCMYEEYKYFIEWLESLPFEKAKKIAPEVGINVAYSVPKELVRGKGSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHCCEEEHHCHHHHHCCCCCE
LGVPGRLRLTPRGLRSCCNPWWGQADHTARLLLEAQKYNPKLRVAMNIRYSPENVAALSG
EECCCCEEECHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEEECCCCCEEECC
AGYEVYELKRETEPPGEESMRWAAKKAYSDLGKVPDVIYDKGFYGKEAMIRLLTEGLEEL
CCCCEEEHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHH
KEMLRVVLQI
HHHHHHHHCC
>Mature Secondary Structure
MNVKKVAAVGGLDPSGGAGIEMDVKVGSAVGVHVHPVATAITYQTPTEFYGSKCTDIEVL
CCCHHEEHCCCCCCCCCCCEEEEEEECCEECEEEECCEEEEEECCCHHHHCCCCCCHHHH
EGQLKAVKGVRNWKLGMLCNERIAETLLKYLEGVVVVDPVLKATAGGTLYEGSLETYKKI
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCEEEECHHHHHCCCCCEEECCHHHHHHH
LSVSFAVTPNALEAEALTGLRVRTVDDAIRAAEALAEAGPKLVVITGGHLEIFADVIYYD
HHHHEEECCCCCCHHHHCCCEEEHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHC
GYVEVIRGTRKVTSFHGSGSFMAMALASYLALGEAPVVAARKAVGYTRVAHLFSQGKEAL
CHHHHHHCCHHHEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHH
DPLYQMRYDAVRHCMYEEYKYFIEWLESLPFEKAKKIAPEVGINVAYSVPKELVRGKGSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHCCEEEHHCHHHHHCCCCCE
LGVPGRLRLTPRGLRSCCNPWWGQADHTARLLLEAQKYNPKLRVAMNIRYSPENVAALSG
EECCCCEEECHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEEECCCCCEEECC
AGYEVYELKRETEPPGEESMRWAAKKAYSDLGKVPDVIYDKGFYGKEAMIRLLTEGLEEL
CCCCEEEHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHH
KEMLRVVLQI
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 4-amino-2-methyl-5-phosphomethylpyrimidine

Specific reaction: ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]