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The map label for this gene is hemC

Identifier: 156937477

GI number: 156937477

Start: 616503

End: 617417

Strand: Direct

Name: hemC

Synonym: Igni_0684

Alternate gene names: 156937477

Gene position: 616503-617417 (Clockwise)

Preceding gene: 156937476

Following gene: 156937478

Centisome position: 47.51

GC content: 55.74

Gene sequence:

>915_bases
TTGAAAATCAAGATAGCCGCGCGCGGGAGCAAACTCAGCTTAAAGCAAGTATCCATGTTCACCTCCTACCTCCTCAAGTT
CTTTCCCGACTTGGAGTACGAAGTGATAACAGTCAAAACCACCGGGGACAAGGCCAACGCCCCTTTCGAGGAGCTCGCCA
AAAGGGGCCTCACCGGCCTCTTCGAGAAGGAAGTTAACAAGGCCGTATTGGAGGGGAAGGCCGACGTGGCGGTCCACTCA
CTCAAGGACTTACCCACAGAACTGGACCCTAGGTTAGAAATAGCGGCCTTCTTGCCCCGCGACCCCCCTTACGACGTCTT
GATCTCCAGGGCCGGCAACTACGACATATTTGACCTCCCCAAAGGCTCCGTGGTGGGGACCTCCTCGGCCCGCAGGAAGG
CTTTAATCAAGAACTTGAGACCCGATCTCGTAGTAAAGGACTTGAGGGGCAACGTGGACACGCGCTTGGAAAAGCTGAGG
AGGGGAGAGTACGACGCCATAGTTCTGGCCGAGGCGGGGGTGTCTAGGTTGGGACTTAATGTCGATTACGTTAGGCTCGA
TTGGAGGCTCTTCCCTCCCTCGCCCGGCCAAGGGATAATTGTAGCTGTAACTAGAAAGGGTAGCGAAATATCTGACCTGT
TGAAGTCCATAAGCGACGTGAAAAGCGAGAAGCTCGCAACCGCCGAAAGGACCGTGTTGAAAGAGTTCGGTGGGGGTTGT
TTCGTAGCGTTGGGGGCCATAGCTTTCGAAGAGGGAAGTTTGATTAGGCTGAGGGCTACGGTCTTAAGCCCCAGCGGGAG
GGAGAGGGTAGACGTGGAGCTGATAGGCAAGGGCCCGGAGGAGGTAGGGATGAGGGCAGCAGAACGCTTGAAGGCCCTAA
ACCCGATGAAGACTACGGTGGGTTCCGAGGAGTGA

Upstream 100 bases:

>100_bases
CGCCGAGCTTGTTCGAAGCGTGGTTCACCTCCGCGGCTCACGGGGAGGACGTGGTCAACAAGACGTTGGAGGCCTTATCC
GAAGCGCTAAAGGTGATATC

Downstream 100 bases:

>100_bases
CGAAGTTCGCTAGGGCTGAGGCTCCTCGCTTTGGCCCCTCTTCACCCCAAACAGTCGGGAGGCCCGGTCCGAGGTTAGAC
CGGGCCCGTTTCCAGTCATC

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MKIKIAARGSKLSLKQVSMFTSYLLKFFPDLEYEVITVKTTGDKANAPFEELAKRGLTGLFEKEVNKAVLEGKADVAVHS
LKDLPTELDPRLEIAAFLPRDPPYDVLISRAGNYDIFDLPKGSVVGTSSARRKALIKNLRPDLVVKDLRGNVDTRLEKLR
RGEYDAIVLAEAGVSRLGLNVDYVRLDWRLFPPSPGQGIIVAVTRKGSEISDLLKSISDVKSEKLATAERTVLKEFGGGC
FVALGAIAFEEGSLIRLRATVLSPSGRERVDVELIGKGPEEVGMRAAERLKALNPMKTTVGSEE

Sequences:

>Translated_304_residues
MKIKIAARGSKLSLKQVSMFTSYLLKFFPDLEYEVITVKTTGDKANAPFEELAKRGLTGLFEKEVNKAVLEGKADVAVHS
LKDLPTELDPRLEIAAFLPRDPPYDVLISRAGNYDIFDLPKGSVVGTSSARRKALIKNLRPDLVVKDLRGNVDTRLEKLR
RGEYDAIVLAEAGVSRLGLNVDYVRLDWRLFPPSPGQGIIVAVTRKGSEISDLLKSISDVKSEKLATAERTVLKEFGGGC
FVALGAIAFEEGSLIRLRATVLSPSGRERVDVELIGKGPEEVGMRAAERLKALNPMKTTVGSEE
>Mature_304_residues
MKIKIAARGSKLSLKQVSMFTSYLLKFFPDLEYEVITVKTTGDKANAPFEELAKRGLTGLFEKEVNKAVLEGKADVAVHS
LKDLPTELDPRLEIAAFLPRDPPYDVLISRAGNYDIFDLPKGSVVGTSSARRKALIKNLRPDLVVKDLRGNVDTRLEKLR
RGEYDAIVLAEAGVSRLGLNVDYVRLDWRLFPPSPGQGIIVAVTRKGSEISDLLKSISDVKSEKLATAERTVLKEFGGGC
FVALGAIAFEEGSLIRLRATVLSPSGRERVDVELIGKGPEEVGMRAAERLKALNPMKTTVGSEE

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI20149500, Length=261, Percent_Identity=36.3984674329502, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI66933009, Length=261, Percent_Identity=36.3984674329502, Blast_Score=135, Evalue=3e-32,
Organism=Escherichia coli, GI48994974, Length=297, Percent_Identity=40.4040404040404, Blast_Score=169, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6319996, Length=284, Percent_Identity=33.0985915492958, Blast_Score=129, Evalue=4e-31,
Organism=Drosophila melanogaster, GI20130425, Length=248, Percent_Identity=36.6935483870968, Blast_Score=135, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_IGNH4 (A8AAB4)

Other databases:

- EMBL:   CP000816
- RefSeq:   YP_001435273.1
- ProteinModelPortal:   A8AAB4
- SMR:   A8AAB4
- STRING:   A8AAB4
- GeneID:   5562426
- GenomeReviews:   CP000816_GR
- KEGG:   iho:Igni_0684
- eggNOG:   arNOG08614
- HOGENOM:   HBG624842
- OMA:   RATEVFD
- ProtClustDB:   PRK00072
- BioCyc:   IHOS453591:IGNI_0684-MONOMER
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 33281; Mature: 33281

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKIAARGSKLSLKQVSMFTSYLLKFFPDLEYEVITVKTTGDKANAPFEELAKRGLTGL
CEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHCCCHHH
FEKEVNKAVLEGKADVAVHSLKDLPTELDPRLEIAAFLPRDPPYDVLISRAGNYDIFDLP
HHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHEECCCCCCEEECC
KGSVVGTSSARRKALIKNLRPDLVVKDLRGNVDTRLEKLRRGEYDAIVLAEAGVSRLGLN
CCCEECCCHHHHHHHHHHCCCCCEEHHHCCCHHHHHHHHHCCCCCEEEEECCCHHHHCCC
VDYVRLDWRLFPPSPGQGIIVAVTRKGSEISDLLKSISDVKSEKLATAERTVLKEFGGGC
EEEEEEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
FVALGAIAFEEGSLIRLRATVLSPSGRERVDVELIGKGPEEVGMRAAERLKALNPMKTTV
EEEEHHHEECCCCEEEEEEEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCHHHHC
GSEE
CCCC
>Mature Secondary Structure
MKIKIAARGSKLSLKQVSMFTSYLLKFFPDLEYEVITVKTTGDKANAPFEELAKRGLTGL
CEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHCCCHHH
FEKEVNKAVLEGKADVAVHSLKDLPTELDPRLEIAAFLPRDPPYDVLISRAGNYDIFDLP
HHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHEECCCCCCEEECC
KGSVVGTSSARRKALIKNLRPDLVVKDLRGNVDTRLEKLRRGEYDAIVLAEAGVSRLGLN
CCCEECCCHHHHHHHHHHCCCCCEEHHHCCCHHHHHHHHHCCCCCEEEEECCCHHHHCCC
VDYVRLDWRLFPPSPGQGIIVAVTRKGSEISDLLKSISDVKSEKLATAERTVLKEFGGGC
EEEEEEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
FVALGAIAFEEGSLIRLRATVLSPSGRERVDVELIGKGPEEVGMRAAERLKALNPMKTTV
EEEEHHHEECCCCEEEEEEEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCHHHHC
GSEE
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA