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The map label for this gene is rio1 [H]

Identifier: 156937468

GI number: 156937468

Start: 608834

End: 609736

Strand: Reverse

Name: rio1 [H]

Synonym: Igni_0675

Alternate gene names: 156937468

Gene position: 609736-608834 (Counterclockwise)

Preceding gene: 156937469

Following gene: 156937467

Centisome position: 46.99

GC content: 52.05

Gene sequence:

>903_bases
ATGAAAAAAGTAGAGGAAGAAGTCAAGGCCCCCCAAAAGGAAGAAGAGGTAGAAGAACAACTTGAAGAGGAAGAGGAACT
CGAAGAGGGAGAGGAGTTAGAAGAGGAAGTAGAAGAACCCATTCCGAAAGAGGTCGTCCAGAGAAGGAAATACTTGAAGA
GGTACAAGGACAAGGACTTGTTTGAGACTGTCGAAGAAGTCTTCGACATGGCCACGCAGATGGCCGTCTATGAACTCATT
AGAAGAGGTGTCATAGGTGAGCTGAAGGGCGTCATATCGGCCGGCAAGGAGGCTAGGGTCTACTGCGGCAAGAGCCCGCA
AGGCGACGACATAGCCGTCAAGATATACTTAACTACAACTGCGGAGTTCCGCAAGAGCATAAGGAAGTACATCATAGGGG
ACCCCCGCTTCGAACAGATAGCCAACAGAGGGCTTCGTCACTTGATCTACGCGTGGGCGAGGAAGGAGTTCAGAAACTTG
AAGAGGCTGGAGCAAGCCGGAGTTAGGGTGCCCTCCCCCATCGCTGTGTACCGGAACGTGCTAGTTATGGAATTCATAGG
CGAGAACTGCAAGAGGGCCCCCTTATTGGTGGAGCTGGCGAAGCCCGTCAACCAGCTCGACGTAGAGGAGTGGAAGAAGA
TATTCGAGACCGTATACGACTACATGGTTAAGATGTACCAAAAGGCTAGGCTCGTCCACGCGGACCTTAACGAGTACAAC
ATAATGTACTGGAAGGGCGAGCCGGTAATTATAGACGTGAGTCAAGCCGTCCCCATAAACCACCCCTACGCCCACGACTT
CTTGATGCACGACATACAGCAGATACGCCGGTTCTTCTCTTCCGTAGGCGTAGAGGTCCCGAGCGCCGCTGAGATGTACG
CGCGAATAACGGGCTTAGAGTGA

Upstream 100 bases:

>100_bases
AAATAGCCTTGACTGCCCAACAAATGGCACAAGAAGAAGCGGAGGGTGAGGAGGCGTGAGCGAGGAGGAGGTTACCAAGG
CTGAGAAGGTAGAGGACGTC

Downstream 100 bases:

>100_bases
GCCCGAGTACAGTTTCTCTAACATTTATTTAAACTTCATTACCCCTTCTGCGCGAACTCCCGGAGAGCGCCTTTGCCGGC
GAAGAGGATCGTAGTCAGAG

Product: non-specific serine/threonine protein kinase

Products: NA

Alternate protein names: AfRio1 [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MKKVEEEVKAPQKEEEVEEQLEEEEELEEGEELEEEVEEPIPKEVVQRRKYLKRYKDKDLFETVEEVFDMATQMAVYELI
RRGVIGELKGVISAGKEARVYCGKSPQGDDIAVKIYLTTTAEFRKSIRKYIIGDPRFEQIANRGLRHLIYAWARKEFRNL
KRLEQAGVRVPSPIAVYRNVLVMEFIGENCKRAPLLVELAKPVNQLDVEEWKKIFETVYDYMVKMYQKARLVHADLNEYN
IMYWKGEPVIIDVSQAVPINHPYAHDFLMHDIQQIRRFFSSVGVEVPSAAEMYARITGLE

Sequences:

>Translated_300_residues
MKKVEEEVKAPQKEEEVEEQLEEEEELEEGEELEEEVEEPIPKEVVQRRKYLKRYKDKDLFETVEEVFDMATQMAVYELI
RRGVIGELKGVISAGKEARVYCGKSPQGDDIAVKIYLTTTAEFRKSIRKYIIGDPRFEQIANRGLRHLIYAWARKEFRNL
KRLEQAGVRVPSPIAVYRNVLVMEFIGENCKRAPLLVELAKPVNQLDVEEWKKIFETVYDYMVKMYQKARLVHADLNEYN
IMYWKGEPVIIDVSQAVPINHPYAHDFLMHDIQQIRRFFSSVGVEVPSAAEMYARITGLE
>Mature_300_residues
MKKVEEEVKAPQKEEEVEEQLEEEEELEEGEELEEEVEEPIPKEVVQRRKYLKRYKDKDLFETVEEVFDMATQMAVYELI
RRGVIGELKGVISAGKEARVYCGKSPQGDDIAVKIYLTTTAEFRKSIRKYIIGDPRFEQIANRGLRHLIYAWARKEFRNL
KRLEQAGVRVPSPIAVYRNVLVMEFIGENCKRAPLLVELAKPVNQLDVEEWKKIFETVYDYMVKMYQKARLVHADLNEYN
IMYWKGEPVIIDVSQAVPINHPYAHDFLMHDIQQIRRFFSSVGVEVPSAAEMYARITGLE

Specific function: Autophosphorylation of the rio1 protein is not necessary for maintenance of kinase activity. Prefers ATP over GTP. The yeast ortholog is involved in ribosome biogenesis [H]

COG id: COG1718

COG function: function code TD; Serine/threonine protein kinase involved in cell cycle control

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 protein kinase domain [H]

Homologues:

Organism=Homo sapiens, GI23510356, Length=252, Percent_Identity=40.8730158730159, Blast_Score=180, Evalue=1e-45,
Organism=Homo sapiens, GI22325377, Length=259, Percent_Identity=33.5907335907336, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI23510358, Length=150, Percent_Identity=42, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI229331971, Length=210, Percent_Identity=26.6666666666667, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI229331967, Length=210, Percent_Identity=26.6666666666667, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI115532073, Length=242, Percent_Identity=37.1900826446281, Blast_Score=159, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17556977, Length=249, Percent_Identity=34.5381526104418, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71989043, Length=145, Percent_Identity=38.6206896551724, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI32564178, Length=207, Percent_Identity=29.4685990338164, Blast_Score=85, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324693, Length=258, Percent_Identity=38.7596899224806, Blast_Score=143, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6324122, Length=218, Percent_Identity=27.5229357798165, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24662811, Length=251, Percent_Identity=38.2470119521912, Blast_Score=164, Evalue=5e-41,
Organism=Drosophila melanogaster, GI21356879, Length=251, Percent_Identity=38.2470119521912, Blast_Score=164, Evalue=5e-41,
Organism=Drosophila melanogaster, GI19920700, Length=260, Percent_Identity=34.6153846153846, Blast_Score=137, Evalue=9e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011009
- InterPro:   IPR000719
- InterPro:   IPR018934
- InterPro:   IPR000687
- InterPro:   IPR018935 [H]

Pfam domain/function: PF01163 RIO1 [H]

EC number: =2.7.11.1 [H]

Molecular weight: Translated: 35123; Mature: 35123

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS01245 RIO1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVEEEVKAPQKEEEVEEQLEEEEELEEGEELEEEVEEPIPKEVVQRRKYLKRYKDKDL
CCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHH
FETVEEVFDMATQMAVYELIRRGVIGELKGVISAGKEARVYCGKSPQGDDIAVKIYLTTT
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEEECC
AEFRKSIRKYIIGDPRFEQIANRGLRHLIYAWARKEFRNLKRLEQAGVRVPSPIAVYRNV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
LVMEFIGENCKRAPLLVELAKPVNQLDVEEWKKIFETVYDYMVKMYQKARLVHADLNEYN
HHHHHHCCCCCCCHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
IMYWKGEPVIIDVSQAVPINHPYAHDFLMHDIQQIRRFFSSVGVEVPSAAEMYARITGLE
EEEEECCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MKKVEEEVKAPQKEEEVEEQLEEEEELEEGEELEEEVEEPIPKEVVQRRKYLKRYKDKDL
CCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHH
FETVEEVFDMATQMAVYELIRRGVIGELKGVISAGKEARVYCGKSPQGDDIAVKIYLTTT
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEEECC
AEFRKSIRKYIIGDPRFEQIANRGLRHLIYAWARKEFRNLKRLEQAGVRVPSPIAVYRNV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
LVMEFIGENCKRAPLLVELAKPVNQLDVEEWKKIFETVYDYMVKMYQKARLVHADLNEYN
HHHHHHCCCCCCCHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
IMYWKGEPVIIDVSQAVPINHPYAHDFLMHDIQQIRRFFSSVGVEVPSAAEMYARITGLE
EEEEECCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]