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The map label for this gene is hypF [H]

Identifier: 156937467

GI number: 156937467

Start: 606551

End: 608761

Strand: Reverse

Name: hypF [H]

Synonym: Igni_0674

Alternate gene names: 156937467

Gene position: 608761-606551 (Counterclockwise)

Preceding gene: 156937468

Following gene: 156937466

Centisome position: 46.92

GC content: 57.98

Gene sequence:

>2211_bases
TTGCCGGCGAAGAGGATCGTAGTCAGAGGCCTCGTCCAAGGGGTTGGCTTCAGGCCCCAAGTCGCCCGCCTGGCCCGCGG
CTTGAAGTTGAAGGGCTACGTGAAGAACGTGGCTGGAGGTGCAGCTGAGATATACGTAATTGGTGAGGATGAGAAAATCT
CCAAGTTCATTAATTTGCTCAAGAAGTTACCTCCCCCCATTAGAGTAGAGGACCTCCAAGCGGAGGATGCCGAGGAGATG
AGTTTTGATGACTTCATTATATTGAAAAGCGACAAAAGCTCCTACAAACACTCCCAGATACCTCCGGACTTGGGGATATG
TGAGGACTGCTTGAGGGAAATGCTCAACCCCTTCTCGAGAAGGTACATGTACCCGTTGATCTCATGCGCCAAGTGTGGCC
CGAGGTTTTCTATGATCCGTTCTCTGCCCTACGATAGAGAGAACACCTCGATGGCCGAGTTCCCGTTCTGCGCAGAGTGC
GAAAGGGAGTACCGGGACCCGTGGGACCCTAGATACCACGTGCAGGGGTTCGCTTGCGCCAAGTGCGGCCCGAAAGTGAG
GCTCTTGGACTCTGAGGGCCGTACGGTGTTGGTCAGAGATCCAATAAAGGAGGCTGCGAAGCTTCTGGACGAGGGTTTCA
TAATAGCGGTCAAGGGCATGGGCGGCTACCACTTGGCCGCCAAGGCGACGGAGGACGAAGTGGTGGCGGAGCTGCGGAGG
AGGAAGGGGAGGCCTAGGAAGCCCTTCGCGCTGATGGCCCTTAATTCCGAGGTAGCGAATAGAATAGTCGTAGTTGACGA
CGAAGAGCTCTTCAACTCTCCGGAAGCCCCCATAGTCCTCTTCCGCAAGAGAGAGGACGGCCCCTTGAGCGAGCTGGTGG
CGCCCGGCCACGCCTTAGTGGGGGTCATGAGGGCCTACACCGGCCTCCATTACTTGCTGCTCAACTACACTGCCGACAAG
TTTTCCATTATGACTTCAGCAAACCCCTCGGGGAAGCCCACGTGTATAACGAAGGAGTGCGCACTAGGGATGGCAGACTA
CGTCTTAGAGCACGATAGGGAAATAGTCCACCGGGTGGACGACAGCGTAGTTAGGAGGAGCGCCGGCGAGAGGCTCTTCT
TGAGGAGGAGCAGGGGCTATGCCCCCGCTTGGCTGGAGGCGGATGAAGAGCTGCTCAGCGGCGCCTCAGTGGGTGCGGAG
CTTCAGAACGCGGGAGGAATTTCCTTTTCCAACAAAGTGGTCTTAACCCAATACATCGGCGACACCGACGAATACGATAA
CTTGAGATTTATGGAAGAAGAGTTGAGGTGGTTATTGAAACAATATTCCGTTACCCCGGAGTTCGTAGCGGCGGACCTCC
ACCCCCGCTACAGCAGCAAGCTCCTAGCGCTGAGCTTGGCGGAGGAGTTCGGAGCAGAGCTGGTAGAGGTCCAACACCAC
CACGCCCACGCGGCCTCGGTCATGGCGGAGGCGCGCTTGGAGGAGGCGGCCGCAATAGTGGTGGACGGCACGGGCTATGG
CTTGGACGGGAACTCTTGGGGAGGGGAGGTCTTGAGGGTAAACAGGGAGGACTTCGAGAGGGTTGCCCACTTAGAGTACT
TCCCCTTGCCGGGCGGAGACAGGGCGGTCAAGTACCCGGCGAGGGCTTTAATGGGCCTCTTGTATGCAGCCGGCGAGGAC
TTAGAGAAGTGGAAAGGCAAGCTCGCGAGGGCCCTCCCGGGAGGGGAGGGAGAGTTCGAGGTGGCTCGCAAGGTTCTCGG
GAGGAGCGTGCTAACCTCCTCCTTAGGTAGGACCTTGGACGCGTTCGCGGCCCTCCTCGGGGTCGCCTACGAGAGGAGTT
ACGAGGGCGAGCCCGCGATGCTCTTGGAGGCGGCCTCCCTCGGGGGCAAACCATTAACAAAGCTAGACTTAATCGATGGA
AACGTTATCAAGGTGACTGAGCTTCTGAGGTGGGCCGTCGAGGCCCTTGATAGCGGCAAAAAGTTAAGGGACGTAGCGTT
TACAATCCAGTACAACCTGGGCTACAACATGGCCCTTAAGGCGGCGGAGCTCGGACTCCCGGTGGTGGTTTCCGGCGGCG
CCGCAGTTAACGAGCCCTTCTTGTTGGGCGTCAAAGAAGTAGTGAAACCCCTCTTGCCACACAAGGTCCCCCCGGGGGAC
GGGGGGATAGCCTTGGGCCAGCTGATAATAGCGTCTAGGAAGCTGAAGTAG

Upstream 100 bases:

>100_bases
GTACGCGCGAATAACGGGCTTAGAGTGAGCCCGAGTACAGTTTCTCTAACATTTATTTAAACTTCATTACCCCTTCTGCG
CGAACTCCCGGAGAGCGCCT

Downstream 100 bases:

>100_bases
TTTCACCGTCCACTTAGTAAAGAAAGTCATTATAGCGGTAAACGGTCTCTAAGGAACGGAGTGAGGCTTTGCCCGAACGC
GTGGTGTTCCTCGGCGGGGG

Product: (NiFe) hydrogenase maturation protein HypF

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]

Number of amino acids: Translated: 736; Mature: 735

Protein sequence:

>736_residues
MPAKRIVVRGLVQGVGFRPQVARLARGLKLKGYVKNVAGGAAEIYVIGEDEKISKFINLLKKLPPPIRVEDLQAEDAEEM
SFDDFIILKSDKSSYKHSQIPPDLGICEDCLREMLNPFSRRYMYPLISCAKCGPRFSMIRSLPYDRENTSMAEFPFCAEC
EREYRDPWDPRYHVQGFACAKCGPKVRLLDSEGRTVLVRDPIKEAAKLLDEGFIIAVKGMGGYHLAAKATEDEVVAELRR
RKGRPRKPFALMALNSEVANRIVVVDDEELFNSPEAPIVLFRKREDGPLSELVAPGHALVGVMRAYTGLHYLLLNYTADK
FSIMTSANPSGKPTCITKECALGMADYVLEHDREIVHRVDDSVVRRSAGERLFLRRSRGYAPAWLEADEELLSGASVGAE
LQNAGGISFSNKVVLTQYIGDTDEYDNLRFMEEELRWLLKQYSVTPEFVAADLHPRYSSKLLALSLAEEFGAELVEVQHH
HAHAASVMAEARLEEAAAIVVDGTGYGLDGNSWGGEVLRVNREDFERVAHLEYFPLPGGDRAVKYPARALMGLLYAAGED
LEKWKGKLARALPGGEGEFEVARKVLGRSVLTSSLGRTLDAFAALLGVAYERSYEGEPAMLLEAASLGGKPLTKLDLIDG
NVIKVTELLRWAVEALDSGKKLRDVAFTIQYNLGYNMALKAAELGLPVVVSGGAAVNEPFLLGVKEVVKPLLPHKVPPGD
GGIALGQLIIASRKLK

Sequences:

>Translated_736_residues
MPAKRIVVRGLVQGVGFRPQVARLARGLKLKGYVKNVAGGAAEIYVIGEDEKISKFINLLKKLPPPIRVEDLQAEDAEEM
SFDDFIILKSDKSSYKHSQIPPDLGICEDCLREMLNPFSRRYMYPLISCAKCGPRFSMIRSLPYDRENTSMAEFPFCAEC
EREYRDPWDPRYHVQGFACAKCGPKVRLLDSEGRTVLVRDPIKEAAKLLDEGFIIAVKGMGGYHLAAKATEDEVVAELRR
RKGRPRKPFALMALNSEVANRIVVVDDEELFNSPEAPIVLFRKREDGPLSELVAPGHALVGVMRAYTGLHYLLLNYTADK
FSIMTSANPSGKPTCITKECALGMADYVLEHDREIVHRVDDSVVRRSAGERLFLRRSRGYAPAWLEADEELLSGASVGAE
LQNAGGISFSNKVVLTQYIGDTDEYDNLRFMEEELRWLLKQYSVTPEFVAADLHPRYSSKLLALSLAEEFGAELVEVQHH
HAHAASVMAEARLEEAAAIVVDGTGYGLDGNSWGGEVLRVNREDFERVAHLEYFPLPGGDRAVKYPARALMGLLYAAGED
LEKWKGKLARALPGGEGEFEVARKVLGRSVLTSSLGRTLDAFAALLGVAYERSYEGEPAMLLEAASLGGKPLTKLDLIDG
NVIKVTELLRWAVEALDSGKKLRDVAFTIQYNLGYNMALKAAELGLPVVVSGGAAVNEPFLLGVKEVVKPLLPHKVPPGD
GGIALGQLIIASRKLK
>Mature_735_residues
PAKRIVVRGLVQGVGFRPQVARLARGLKLKGYVKNVAGGAAEIYVIGEDEKISKFINLLKKLPPPIRVEDLQAEDAEEMS
FDDFIILKSDKSSYKHSQIPPDLGICEDCLREMLNPFSRRYMYPLISCAKCGPRFSMIRSLPYDRENTSMAEFPFCAECE
REYRDPWDPRYHVQGFACAKCGPKVRLLDSEGRTVLVRDPIKEAAKLLDEGFIIAVKGMGGYHLAAKATEDEVVAELRRR
KGRPRKPFALMALNSEVANRIVVVDDEELFNSPEAPIVLFRKREDGPLSELVAPGHALVGVMRAYTGLHYLLLNYTADKF
SIMTSANPSGKPTCITKECALGMADYVLEHDREIVHRVDDSVVRRSAGERLFLRRSRGYAPAWLEADEELLSGASVGAEL
QNAGGISFSNKVVLTQYIGDTDEYDNLRFMEEELRWLLKQYSVTPEFVAADLHPRYSSKLLALSLAEEFGAELVEVQHHH
AHAASVMAEARLEEAAAIVVDGTGYGLDGNSWGGEVLRVNREDFERVAHLEYFPLPGGDRAVKYPARALMGLLYAAGEDL
EKWKGKLARALPGGEGEFEVARKVLGRSVLTSSLGRTLDAFAALLGVAYERSYEGEPAMLLEAASLGGKPLTKLDLIDGN
VIKVTELLRWAVEALDSGKKLRDVAFTIQYNLGYNMALKAAELGLPVVVSGGAAVNEPFLLGVKEVVKPLLPHKVPPGDG
GIALGQLIIASRKLK

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=752, Percent_Identity=35.7712765957447, Blast_Score=338, Evalue=5e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 81128; Mature: 80997

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAKRIVVRGLVQGVGFRPQVARLARGLKLKGYVKNVAGGAAEIYVIGEDEKISKFINLL
CCCHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHCCCCCEEEEEECCCHHHHHHHHHH
KKLPPPIRVEDLQAEDAEEMSFDDFIILKSDKSSYKHSQIPPDLGICEDCLREMLNPFSR
HHCCCCCEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
RYMYPLISCAKCGPRFSMIRSLPYDRENTSMAEFPFCAECEREYRDPWDPRYHVQGFACA
HHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEECCEEEC
KCGPKVRLLDSEGRTVLVRDPIKEAAKLLDEGFIIAVKGMGGYHLAAKATEDEVVAELRR
CCCCEEEEEECCCCEEEEECCHHHHHHHHCCCCEEEEECCCCEEEEECCCHHHHHHHHHH
RKGRPRKPFALMALNSEVANRIVVVDDEELFNSPEAPIVLFRKREDGPLSELVAPGHALV
HCCCCCCCEEEEEECHHHCCEEEEEECHHHHCCCCCCEEEEEECCCCCHHHHHCCHHHHH
GVMRAYTGLHYLLLNYTADKFSIMTSANPSGKPTCITKECALGMADYVLEHDREIVHRVD
HHHHHHHHHEEEEEEECCCCEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
DSVVRRSAGERLFLRRSRGYAPAWLEADEELLSGASVGAELQNAGGISFSNKVVLTQYIG
HHHHHHCCCCEEEEECCCCCCCHHHCCCHHHHCCCCCCCHHHCCCCCCCCCCEEEEEECC
DTDEYDNLRFMEEELRWLLKQYSVTPEFVAADLHPRYSSKLLALSLAEEFGAELVEVQHH
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHEECCCCCHHHHHHHHHHHHHHCHHHHHHHHH
HAHAASVMAEARLEEAAAIVVDGTGYGLDGNSWGGEVLRVNREDFERVAHLEYFPLPGGD
HHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEEECHHHHHHHHHCEEECCCCCC
RAVKYPARALMGLLYAAGEDLEKWKGKLARALPGGEGEFEVARKVLGRSVLTSSLGRTLD
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AFAALLGVAYERSYEGEPAMLLEAASLGGKPLTKLDLIDGNVIKVTELLRWAVEALDSGK
HHHHHHHHHHHCCCCCCCCEEEEEHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCC
KLRDVAFTIQYNLGYNMALKAAELGLPVVVSGGAAVNEPFLLGVKEVVKPLLPHKVPPGD
CEEEEEEEEEEECCCCEEEEHHHCCCCEEEECCCCCCCCHHCCHHHHHHHHCCCCCCCCC
GGIALGQLIIASRKLK
CCHHHHHHHHHHCCCC
>Mature Secondary Structure 
PAKRIVVRGLVQGVGFRPQVARLARGLKLKGYVKNVAGGAAEIYVIGEDEKISKFINLL
CCHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHCCCCCEEEEEECCCHHHHHHHHHH
KKLPPPIRVEDLQAEDAEEMSFDDFIILKSDKSSYKHSQIPPDLGICEDCLREMLNPFSR
HHCCCCCEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
RYMYPLISCAKCGPRFSMIRSLPYDRENTSMAEFPFCAECEREYRDPWDPRYHVQGFACA
HHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEECCEEEC
KCGPKVRLLDSEGRTVLVRDPIKEAAKLLDEGFIIAVKGMGGYHLAAKATEDEVVAELRR
CCCCEEEEEECCCCEEEEECCHHHHHHHHCCCCEEEEECCCCEEEEECCCHHHHHHHHHH
RKGRPRKPFALMALNSEVANRIVVVDDEELFNSPEAPIVLFRKREDGPLSELVAPGHALV
HCCCCCCCEEEEEECHHHCCEEEEEECHHHHCCCCCCEEEEEECCCCCHHHHHCCHHHHH
GVMRAYTGLHYLLLNYTADKFSIMTSANPSGKPTCITKECALGMADYVLEHDREIVHRVD
HHHHHHHHHEEEEEEECCCCEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
DSVVRRSAGERLFLRRSRGYAPAWLEADEELLSGASVGAELQNAGGISFSNKVVLTQYIG
HHHHHHCCCCEEEEECCCCCCCHHHCCCHHHHCCCCCCCHHHCCCCCCCCCCEEEEEECC
DTDEYDNLRFMEEELRWLLKQYSVTPEFVAADLHPRYSSKLLALSLAEEFGAELVEVQHH
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHEECCCCCHHHHHHHHHHHHHHCHHHHHHHHH
HAHAASVMAEARLEEAAAIVVDGTGYGLDGNSWGGEVLRVNREDFERVAHLEYFPLPGGD
HHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEEECHHHHHHHHHCEEECCCCCC
RAVKYPARALMGLLYAAGEDLEKWKGKLARALPGGEGEFEVARKVLGRSVLTSSLGRTLD
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AFAALLGVAYERSYEGEPAMLLEAASLGGKPLTKLDLIDGNVIKVTELLRWAVEALDSGK
HHHHHHHHHHHCCCCCCCCEEEEEHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCC
KLRDVAFTIQYNLGYNMALKAAELGLPVVVSGGAAVNEPFLLGVKEVVKPLLPHKVPPGD
CEEEEEEEEEEECCCCEEEEHHHCCCCEEEECCCCCCCCHHCCHHHHHHHHCCCCCCCCC
GGIALGQLIIASRKLK
CCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]