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The map label for this gene is argG

Identifier: 156937428

GI number: 156937428

Start: 571309

End: 572499

Strand: Direct

Name: argG

Synonym: Igni_0635

Alternate gene names: 156937428

Gene position: 571309-572499 (Clockwise)

Preceding gene: 156937427

Following gene: 156937430

Centisome position: 44.03

GC content: 52.9

Gene sequence:

>1191_bases
GTGTCTAAGATCGTTCTAGCCTACTCGGGAGGGCTGGACACGTCGGTTGCAGTGGCGTGGCTGAAGGAGAAGTTCGGGGC
GGAAGTAATAACTGTGACGGTAGATGTCGGACAAAAGGAAGATTTCAAGGAGATAGAGGAGAGGGCTTACAAGGCTGGCG
CATCCAAGCATTACCTAATTGATGCTAAAGAAGAGTTCGTAAACAAGTACATAAAGCCAGCAATAATAGCGAACGCACTC
TACGAGGAAGTCTACCCCTTAGGCACCGCGCTCGCCAGGCCCCTCATAGGGGAGAAGGTAATAGAAGTAGCCAGAAAGGA
AGGGGCGGACGCGGTGGCTCACGGGGCCACGAGCAAGGGCAACGACCAAGTGCGGTTTGAAGCGGCCTTCAAGGCCTTAG
CCCCAGAAATAAAGATAATAGCTCCTATAAGGATGTGGGGGATGAACAGAAAGGAAGAATACGAGTTCGCTAAGAAGAAG
GGTATCCCGATAAGTGCTGAGTCCAAGAAATATAGCATAGACGAGAACTTGTGGTCTAGAAGCATAGAGGGTGGCGAGCT
GGAGGACCCTTGGCAAGAGCCGCCGGAGGAGGCCTTCGAGTGGACCGTCCCTCCGGAGAAGGCGCCAGAGGAGCCCGCGT
ACTTAACGATCACCTTTGAGAAGGGTGTCCCGGTGGCCCTAAACGGCAAGAGGATGGAATTGATTGAGATAATAGATACC
CTCAACAAGTTAGCAGGCCAGCACGGGTACGGACGCGTCGACCACATGGAGAACAGGGTAGTCGGGCTTAAGAGCAGAGA
AGTTTACGAGGCGCCTGCCGCCCTCACTATAATAAATTCTAAGAAGGACTTGGAGAAGCTCGTGCTCAACAAGAGGGAGT
ACAGAATTAAAAGGATGCTCGACTCTTACTGGGCGGACCTAGTTTACGACGGACTCTGGTTCGACCCCATAAGGGTGGCC
TTGGACAATATGTTGAGCGAACTGAGCGAGAACGTTAACGGCGACGTGAAGGTGAAGTTATACAAGGGCTCCTTGCGCGT
CGTAGGGAGGAGGAGTCCAAACGCGATCTACGACAAAGCGATAAGCAGCTACGAGAGCGAGTGGTTCCCGAGCGACGAGA
TGGCCAGAGGCTTCATAGACGCTTGGCTTATGGACAGCGTCGTCAGCTTTCGGAAGAGGTACTTGAAGTGA

Upstream 100 bases:

>100_bases
GCGTTGCCGATTCTTTGATGAATTGAGCAAAAGAATCCTCAGCATACTATATAATCTGCACTAGAAGGTCCTTTATGGGC
GCGCGGCTGTGAAGGGAGAA

Downstream 100 bases:

>100_bases
GGTACTTCTTAACTGGGACCTCGAAGGGGGGCTTCTCTACCTTAACTTCCTTAACGTCTCCGAATCCCAAGCTTACGTTC
GAGCCTAACCCGAAGTAGTG

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSKIVLAYSGGLDTSVAVAWLKEKFGAEVITVTVDVGQKEDFKEIEERAYKAGASKHYLIDAKEEFVNKYIKPAIIANAL
YEEVYPLGTALARPLIGEKVIEVARKEGADAVAHGATSKGNDQVRFEAAFKALAPEIKIIAPIRMWGMNRKEEYEFAKKK
GIPISAESKKYSIDENLWSRSIEGGELEDPWQEPPEEAFEWTVPPEKAPEEPAYLTITFEKGVPVALNGKRMELIEIIDT
LNKLAGQHGYGRVDHMENRVVGLKSREVYEAPAALTIINSKKDLEKLVLNKREYRIKRMLDSYWADLVYDGLWFDPIRVA
LDNMLSELSENVNGDVKVKLYKGSLRVVGRRSPNAIYDKAISSYESEWFPSDEMARGFIDAWLMDSVVSFRKRYLK

Sequences:

>Translated_396_residues
MSKIVLAYSGGLDTSVAVAWLKEKFGAEVITVTVDVGQKEDFKEIEERAYKAGASKHYLIDAKEEFVNKYIKPAIIANAL
YEEVYPLGTALARPLIGEKVIEVARKEGADAVAHGATSKGNDQVRFEAAFKALAPEIKIIAPIRMWGMNRKEEYEFAKKK
GIPISAESKKYSIDENLWSRSIEGGELEDPWQEPPEEAFEWTVPPEKAPEEPAYLTITFEKGVPVALNGKRMELIEIIDT
LNKLAGQHGYGRVDHMENRVVGLKSREVYEAPAALTIINSKKDLEKLVLNKREYRIKRMLDSYWADLVYDGLWFDPIRVA
LDNMLSELSENVNGDVKVKLYKGSLRVVGRRSPNAIYDKAISSYESEWFPSDEMARGFIDAWLMDSVVSFRKRYLK
>Mature_395_residues
SKIVLAYSGGLDTSVAVAWLKEKFGAEVITVTVDVGQKEDFKEIEERAYKAGASKHYLIDAKEEFVNKYIKPAIIANALY
EEVYPLGTALARPLIGEKVIEVARKEGADAVAHGATSKGNDQVRFEAAFKALAPEIKIIAPIRMWGMNRKEEYEFAKKKG
IPISAESKKYSIDENLWSRSIEGGELEDPWQEPPEEAFEWTVPPEKAPEEPAYLTITFEKGVPVALNGKRMELIEIIDTL
NKLAGQHGYGRVDHMENRVVGLKSREVYEAPAALTIINSKKDLEKLVLNKREYRIKRMLDSYWADLVYDGLWFDPIRVAL
DNMLSELSENVNGDVKVKLYKGSLRVVGRRSPNAIYDKAISSYESEWFPSDEMARGFIDAWLMDSVVSFRKRYLK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=390, Percent_Identity=43.0769230769231, Blast_Score=292, Evalue=3e-79,
Organism=Homo sapiens, GI16950633, Length=390, Percent_Identity=43.0769230769231, Blast_Score=292, Evalue=3e-79,
Organism=Escherichia coli, GI1789563, Length=371, Percent_Identity=29.9191374663073, Blast_Score=133, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6324514, Length=399, Percent_Identity=40.3508771929825, Blast_Score=281, Evalue=1e-76,
Organism=Drosophila melanogaster, GI21358151, Length=393, Percent_Identity=43.0025445292621, Blast_Score=286, Evalue=2e-77,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_IGNH4 (A8AA65)

Other databases:

- EMBL:   CP000816
- RefSeq:   YP_001435224.1
- ProteinModelPortal:   A8AA65
- SMR:   A8AA65
- STRING:   A8AA65
- GeneID:   5563088
- GenomeReviews:   CP000816_GR
- KEGG:   iho:Igni_0635
- eggNOG:   arNOG04108
- HOGENOM:   HBG335267
- OMA:   KRVILAY
- ProtClustDB:   PRK00509
- BioCyc:   IHOS453591:IGNI_0635-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 44839; Mature: 44708

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 85-85 BINDING 115-115 BINDING 117-117 BINDING 121-121 BINDING 121-121 BINDING 122-122 BINDING 125-125 BINDING 172-172 BINDING 181-181 BINDING 257-257 BINDING 269-269

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIVLAYSGGLDTSVAVAWLKEKFGAEVITVTVDVGQKEDFKEIEERAYKAGASKHYLI
CCEEEEEECCCCCHHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEE
DAKEEFVNKYIKPAIIANALYEEVYPLGTALARPLIGEKVIEVARKEGADAVAHGATSKG
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCC
NDQVRFEAAFKALAPEIKIIAPIRMWGMNRKEEYEFAKKKGIPISAESKKYSIDENLWSR
CCCCHHHHHHHHHCCCEEEEEEHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHC
SIEGGELEDPWQEPPEEAFEWTVPPEKAPEEPAYLTITFEKGVPVALNGKRMELIEIIDT
CCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEECCCHHHHHHHHHH
LNKLAGQHGYGRVDHMENRVVGLKSREVYEAPAALTIINSKKDLEKLVLNKREYRIKRML
HHHHHCCCCCCCHHHHCCCEECCCCCHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
DSYWADLVYDGLWFDPIRVALDNMLSELSENVNGDVKVKLYKGSLRVVGRRSPNAIYDKA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEECCCCCHHHHHH
ISSYESEWFPSDEMARGFIDAWLMDSVVSFRKRYLK
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKIVLAYSGGLDTSVAVAWLKEKFGAEVITVTVDVGQKEDFKEIEERAYKAGASKHYLI
CEEEEEECCCCCHHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEE
DAKEEFVNKYIKPAIIANALYEEVYPLGTALARPLIGEKVIEVARKEGADAVAHGATSKG
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCC
NDQVRFEAAFKALAPEIKIIAPIRMWGMNRKEEYEFAKKKGIPISAESKKYSIDENLWSR
CCCCHHHHHHHHHCCCEEEEEEHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHC
SIEGGELEDPWQEPPEEAFEWTVPPEKAPEEPAYLTITFEKGVPVALNGKRMELIEIIDT
CCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEECCCHHHHHHHHHH
LNKLAGQHGYGRVDHMENRVVGLKSREVYEAPAALTIINSKKDLEKLVLNKREYRIKRML
HHHHHCCCCCCCHHHHCCCEECCCCCHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
DSYWADLVYDGLWFDPIRVALDNMLSELSENVNGDVKVKLYKGSLRVVGRRSPNAIYDKA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEECCCCCHHHHHH
ISSYESEWFPSDEMARGFIDAWLMDSVVSFRKRYLK
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA