Click here to switch to the map view.

The map label for this gene is speE [H]

Identifier: 156937426

GI number: 156937426

Start: 569314

End: 570240

Strand: Reverse

Name: speE [H]

Synonym: Igni_0633

Alternate gene names: 156937426

Gene position: 570240-569314 (Counterclockwise)

Preceding gene: 156937429

Following gene: 156937424

Centisome position: 43.95

GC content: 55.02

Gene sequence:

>927_bases
GTGCACAGAGTTCCCTTGAAGACCTTTAATTGGTTCTTAGAGGTCCAAACGCCGTGTTCCATGCACGCCCACGGCCTCTT
GGAGACTTTGTACGTAGGCAAGAGCAAGTACCAAGAGATAGTCGTTGCGAGGCTCTGCAACGTCGGGAAGGCATTAATAC
TAGACGGGAAGATCCAGTCGAGCGAGTACGACGAGTGGATCTACCACGAGTCGCTAGTTCACCCGGTCATGTTGGCCCAC
CCCAACCCCACGGAGGTCCTAATACTGGGCGGTGGCGAGGGGGCCACCTTGAGGGAGGTCTTGAAGCATAAGAGCGTAAA
GAAGGTGACGATGGTTGACTTAGACGAGGAAGTAATTAAAGTCAGTAAAAAGTACTTGCCGGAGTGGCACCGAGGGTCCT
TCGACGACCCTCGGGCAGAGCTCGTTATAGAGGACGGCCGCAAGTTCTTGGAGGAAGCCCCGGAGAACTCTTACGACGTC
GTAATAATAGACCTCGTGGACCCCACTGAGGGCGCGCCTGCAGCTAAGCTCTACACCAAGGAGTTCTACGAATTGGTGAA
GAAGGTCTTGAGGGATGAAGGAATAATGGTCACTCAAGCTACCTCGACTTCCTACACATTGGACGTGTTCACGGTAGTAT
ATAGAACCGTCAAGGAAGTGTTCGAGAAGGCGGGAGGCTACCACTCCTTCGTACAAGCGTTCGACGCGACTTGGGGCTTC
GTCTGGGGCTCCACGGGCCCCGACCCGAGCTCTTTGAGCCCCGACGCAGTCGACCGCCTCATACGGGAGAGGCTTGAAGG
GGAGCTCAAGTACTACGACGGGATTACGCACGTCGGGATGTTCTCGCTGCCAAAACACGTGAGGAAGGCTATGGCTGCGC
AAACGACAGTCAGTACTGACGAGAGGCCCTACCTAGCCTTAGTCTAA

Upstream 100 bases:

>100_bases
AGCTCCTCAGGGTCGTGGACCGGCACTTCCTCTAGCATCTCTATCACCAAAGCCCAGATAACTTCCGGAGCTTTCAAAAG
ACTTAGGGAGGTGAAAAAGG

Downstream 100 bases:

>100_bases
CACAAGTTTTCTTGCCCTTTCTAACAAGCTCGGGTTCGCCTCCAAAGTGCCCCTCACCGCCTCCGTAATCATTTCCACGT
CCGGGTCCTCCACCCCCCTA

Product: spermine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MHRVPLKTFNWFLEVQTPCSMHAHGLLETLYVGKSKYQEIVVARLCNVGKALILDGKIQSSEYDEWIYHESLVHPVMLAH
PNPTEVLILGGGEGATLREVLKHKSVKKVTMVDLDEEVIKVSKKYLPEWHRGSFDDPRAELVIEDGRKFLEEAPENSYDV
VIIDLVDPTEGAPAAKLYTKEFYELVKKVLRDEGIMVTQATSTSYTLDVFTVVYRTVKEVFEKAGGYHSFVQAFDATWGF
VWGSTGPDPSSLSPDAVDRLIRERLEGELKYYDGITHVGMFSLPKHVRKAMAAQTTVSTDERPYLALV

Sequences:

>Translated_308_residues
MHRVPLKTFNWFLEVQTPCSMHAHGLLETLYVGKSKYQEIVVARLCNVGKALILDGKIQSSEYDEWIYHESLVHPVMLAH
PNPTEVLILGGGEGATLREVLKHKSVKKVTMVDLDEEVIKVSKKYLPEWHRGSFDDPRAELVIEDGRKFLEEAPENSYDV
VIIDLVDPTEGAPAAKLYTKEFYELVKKVLRDEGIMVTQATSTSYTLDVFTVVYRTVKEVFEKAGGYHSFVQAFDATWGF
VWGSTGPDPSSLSPDAVDRLIRERLEGELKYYDGITHVGMFSLPKHVRKAMAAQTTVSTDERPYLALV
>Mature_308_residues
MHRVPLKTFNWFLEVQTPCSMHAHGLLETLYVGKSKYQEIVVARLCNVGKALILDGKIQSSEYDEWIYHESLVHPVMLAH
PNPTEVLILGGGEGATLREVLKHKSVKKVTMVDLDEEVIKVSKKYLPEWHRGSFDDPRAELVIEDGRKFLEEAPENSYDV
VIIDLVDPTEGAPAAKLYTKEFYELVKKVLRDEGIMVTQATSTSYTLDVFTVVYRTVKEVFEKAGGYHSFVQAFDATWGF
VWGSTGPDPSSLSPDAVDRLIRERLEGELKYYDGITHVGMFSLPKHVRKAMAAQTTVSTDERPYLALV

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=288, Percent_Identity=33.3333333333333, Blast_Score=162, Evalue=4e-40,
Organism=Escherichia coli, GI1786312, Length=269, Percent_Identity=34.2007434944238, Blast_Score=176, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI25154106, Length=266, Percent_Identity=36.0902255639098, Blast_Score=151, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI212645616, Length=197, Percent_Identity=41.6243654822335, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6323175, Length=289, Percent_Identity=34.2560553633218, Blast_Score=170, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6325326, Length=288, Percent_Identity=36.1111111111111, Blast_Score=160, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24645443, Length=285, Percent_Identity=34.3859649122807, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI21355941, Length=215, Percent_Identity=35.3488372093023, Blast_Score=125, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 34780; Mature: 34780

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRVPLKTFNWFLEVQTPCSMHAHGLLETLYVGKSKYQEIVVARLCNVGKALILDGKIQS
CCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCEEEECCCCCC
SEYDEWIYHESLVHPVMLAHPNPTEVLILGGGEGATLREVLKHKSVKKVTMVDLDEEVIK
CCCHHHHHHHHHHCCEEEECCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEECCCHHHHH
VSKKYLPEWHRGSFDDPRAELVIEDGRKFLEEAPENSYDVVIIDLVDPTEGAPAAKLYTK
HHHHHCCHHHCCCCCCCHHHHEEHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHH
EFYELVKKVLRDEGIMVTQATSTSYTLDVFTVVYRTVKEVFEKAGGYHSFVQAFDATWGF
HHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCE
VWGSTGPDPSSLSPDAVDRLIRERLEGELKYYDGITHVGMFSLPKHVRKAMAAQTTVSTD
EECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
ERPYLALV
CCCEEEEC
>Mature Secondary Structure
MHRVPLKTFNWFLEVQTPCSMHAHGLLETLYVGKSKYQEIVVARLCNVGKALILDGKIQS
CCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCEEEECCCCCC
SEYDEWIYHESLVHPVMLAHPNPTEVLILGGGEGATLREVLKHKSVKKVTMVDLDEEVIK
CCCHHHHHHHHHHCCEEEECCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEECCCHHHHH
VSKKYLPEWHRGSFDDPRAELVIEDGRKFLEEAPENSYDVVIIDLVDPTEGAPAAKLYTK
HHHHHCCHHHCCCCCCCHHHHEEHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHH
EFYELVKKVLRDEGIMVTQATSTSYTLDVFTVVYRTVKEVFEKAGGYHSFVQAFDATWGF
HHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCE
VWGSTGPDPSSLSPDAVDRLIRERLEGELKYYDGITHVGMFSLPKHVRKAMAAQTTVSTD
EECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
ERPYLALV
CCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479 [H]