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The map label for this gene is folE [H]

Identifier: 156937425

GI number: 156937425

Start: 568762

End: 569322

Strand: Direct

Name: folE [H]

Synonym: Igni_0632

Alternate gene names: 156937425

Gene position: 568762-569322 (Clockwise)

Preceding gene: 156937420

Following gene: 156937427

Centisome position: 43.83

GC content: 54.01

Gene sequence:

>561_bases
ATGGAAGAGAACGTCTTGGAGGTTGCTATCAAAAAGTTCTTAGAAGAAATGGGCGAAGACCCCAGCAGGCCGGGCTTGAG
CGAAACCCCCGAGAGGTACGTGAGGGCTCTTAGAGAGCTCTTCAAAGGCTTGGAGACCCCCAAGCCTGAGCTGAAGACCT
TCAAGACCGAGTACCTCCTCGGGGAGAACGACCTAATAGTAGTCAAGAACGTGTCTTTCAGCTCCTTGTGCGAACATCAC
TTGCTGCCCATACTGGGCAGAGCTTCCATCGTGTACCACCCCAAGAGGGACGAGGTGATCGGTTTGAGCAAGATACCGAG
GATAGTGAAGTGGTTAGCCAAGAGGCCTATGTTGCAAGAGAGGTTCACGGCCATGATTGCCGACGAGATAATGAAGGCCG
GCGCGAGGGGCGTTTACGTCCTAGTTAGGGCGAAGCACTCTTGCGTAGCGGTTAGGGGGGTGGAGGACCCGGACGTGGAA
ATGATTACGGAGGCGGTGAGGGGCACTTTGGAGGCGAACCCGAGCTTGTTAGAAAGGGCAAGAAAACTTGTGTTAGACTA
A

Upstream 100 bases:

>100_bases
GCTCTTTTATGAAGTCCTCTCTGTTCAAGAGTCCGCCCTTTAGGCCCCGCGCTTCGACCGCTTTTATGCTGCCTCGGACA
ACCCGAAGGGGAGTTAGGCT

Downstream 100 bases:

>100_bases
GGCTAGGTAGGGCCTCTCGTCAGTACTGACTGTCGTTTGCGCAGCCATAGCCTTCCTCACGTGTTTTGGCAGCGAGAACA
TCCCGACGTGCGTAATCCCG

Product: GTP cyclohydrolase

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MEENVLEVAIKKFLEEMGEDPSRPGLSETPERYVRALRELFKGLETPKPELKTFKTEYLLGENDLIVVKNVSFSSLCEHH
LLPILGRASIVYHPKRDEVIGLSKIPRIVKWLAKRPMLQERFTAMIADEIMKAGARGVYVLVRAKHSCVAVRGVEDPDVE
MITEAVRGTLEANPSLLERARKLVLD

Sequences:

>Translated_186_residues
MEENVLEVAIKKFLEEMGEDPSRPGLSETPERYVRALRELFKGLETPKPELKTFKTEYLLGENDLIVVKNVSFSSLCEHH
LLPILGRASIVYHPKRDEVIGLSKIPRIVKWLAKRPMLQERFTAMIADEIMKAGARGVYVLVRAKHSCVAVRGVEDPDVE
MITEAVRGTLEANPSLLERARKLVLD
>Mature_186_residues
MEENVLEVAIKKFLEEMGEDPSRPGLSETPERYVRALRELFKGLETPKPELKTFKTEYLLGENDLIVVKNVSFSSLCEHH
LLPILGRASIVYHPKRDEVIGLSKIPRIVKWLAKRPMLQERFTAMIADEIMKAGARGVYVLVRAKHSCVAVRGVEDPDVE
MITEAVRGTLEANPSLLERARKLVLD

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=176, Percent_Identity=34.0909090909091, Blast_Score=102, Evalue=2e-22,
Organism=Homo sapiens, GI4503949, Length=176, Percent_Identity=34.0909090909091, Blast_Score=102, Evalue=2e-22,
Organism=Homo sapiens, GI66932970, Length=152, Percent_Identity=34.8684210526316, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI66932972, Length=142, Percent_Identity=35.9154929577465, Blast_Score=83, Evalue=1e-16,
Organism=Escherichia coli, GI1788476, Length=150, Percent_Identity=35.3333333333333, Blast_Score=95, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17560486, Length=180, Percent_Identity=33.8888888888889, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321706, Length=170, Percent_Identity=35.2941176470588, Blast_Score=108, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24656782, Length=176, Percent_Identity=34.0909090909091, Blast_Score=95, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24656796, Length=176, Percent_Identity=34.0909090909091, Blast_Score=94, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24656791, Length=176, Percent_Identity=34.0909090909091, Blast_Score=93, Evalue=1e-19,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 21076; Mature: 21076

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEENVLEVAIKKFLEEMGEDPSRPGLSETPERYVRALRELFKGLETPKPELKTFKTEYLL
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEE
GENDLIVVKNVSFSSLCEHHLLPILGRASIVYHPKRDEVIGLSKIPRIVKWLAKRPMLQE
CCCCEEEEECCCHHHHHHHHHHHHHCCCEEEECCCCCCEECHHHHHHHHHHHHHCCHHHH
RFTAMIADEIMKAGARGVYVLVRAKHSCVAVRGVEDPDVEMITEAVRGTLEANPSLLERA
HHHHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHH
RKLVLD
HHHHCC
>Mature Secondary Structure
MEENVLEVAIKKFLEEMGEDPSRPGLSETPERYVRALRELFKGLETPKPELKTFKTEYLL
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEE
GENDLIVVKNVSFSSLCEHHLLPILGRASIVYHPKRDEVIGLSKIPRIVKWLAKRPMLQE
CCCCEEEEECCCHHHHHHHHHHHHHCCCEEEECCCCCCEECHHHHHHHHHHHHHCCHHHH
RFTAMIADEIMKAGARGVYVLVRAKHSCVAVRGVEDPDVEMITEAVRGTLEANPSLLERA
HHHHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHH
RKLVLD
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA