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The map label for this gene is abfD [H]

Identifier: 156937388

GI number: 156937388

Start: 532213

End: 533751

Strand: Direct

Name: abfD [H]

Synonym: Igni_0595

Alternate gene names: 156937388

Gene position: 532213-533751 (Clockwise)

Preceding gene: 156937387

Following gene: 156937392

Centisome position: 41.02

GC content: 56.73

Gene sequence:

>1539_bases
ATGGGGCTCCGTAAGCCCGAGGAGTACATAAGCGCTCTCAGAGAGAAGAGCAAGAGGGCCGAAATATACGTCCTCGGGGA
GAGGGTAGAGGACGTCACGAAACACCCCTTCACCCTGCCGGCCGTCAAGGCCTTCGAGTGGACCTACAAGGCCCCGTGGG
ACCCCTACTTCTACGACAAAGAGATGGGCGTCCACCTAGCTAAGGCCTACTCGCCGTTCATAAACGAAGAGATAAACCGC
TTCAACCACATCCACCAGAGCCCTCAAGACTTAGCCATTAAGGTCAAGTACTTCAGGAAGCTCTCCCACAAGGCCGGCTC
GTGCTTCCAGAGGTGCGTAGGCTGGGACGCCATAAACACGATAAGCATCATCTCCTATGACATAGACAAATGGTACGAAC
AGAAGGGCGAGAAGCCGGAGCACTTCGAGCACTTCGGCACTTACTACGAGAGGTTCCTCAAGTTCCTCAAGTACGTACAA
AAGGAGGACATCGCGCTCGCCGGAGTCATGACCGACGCCAAGGGAGTGAGGTCCTTGAGGCCCCACGAACAGCCAAACAA
GGACGTTTACGTGCACGTGAAGGAGGTAGCCGACGAAGGCATAATAGTGAGGGGAGCTAAGGCCAACATAACTGGTGTCG
TAGCCAGCGAAGAGATGATTGTAATGCCTACCAGGGCCATGACGGAGAAGGACAAGGACTTTGCCATAGCCTTCGCGGCG
CCGATAGACGCCGAAGGGGTCACCTTGGTAGTCGGCAGGTACACGAACGACACTAGGAGGTTCGAGGAGCCCAACGTGGA
CGCGCTGCCCGGAGTCTTTCCGGCTGAGGCCATAGTCATCTTCGACGACGTGTTCGTCCCGTGGGAGAGGGTGTTCATGT
TTAGGGAGTACCAGTTCGCCGCCGAATTGGTCGAGATATTCTCGAGCTACCACAGGCAAGCCTACGGCGGCTGCAAGCCG
GGCTTAGCGGACGTGATAATAGGCGGCGCTTACCACTTGGCGAAGCAGATGGGCATAGCCAACAAGTGCCACATACGGGA
GAAGCTCACCGAGATGATATTCCTCACGGAAGCCATGCACGCCGGCGGGCTCGCCGCCGCTTGGGAAGGCAAGAAGGCCG
CCTCCGGCACTTACTACGTCGACCAGATGAAGGCCAACGTAACCAAACAGATGGTCACCAGGTTCCCCTACGAAATAGCC
AGGTTGGCCCACGACATAGCCGGCGGCCTGATAGGCACTTTGCCGTCTTACAAGGACTTGATGCACGAGAAGATAGGGAA
GTACTTACAGCAGTACCTCCAGTGCTTGCCCGACTACCCGGCGGAGTGGAGGCACCGCGTGCTCCGCTTGCTGGAGAACA
TAACTTTGAGCGTGGAGTTCCTAATAGAGAGCGTACACGGAGCGGGGAGCCCAGAGGCCCAGAGGATATGGATGAGGAGG
TTCTACCCGTTGGAGGAAATGGAGGATGTCGCCCTTTCCTTGGCCGGCATAAACCCGGAGGAGAAGCCGGAGAGGAAGAA
GCAGAAAGAGCAAACCTAA

Upstream 100 bases:

>100_bases
GCGATGAGGTTTGCACGTCAGGCCGACCGGTGAGGAGGATTGGCCTTTCCGAGCCAAACCCCTTTTACCGCCGAGTCTCT
AACGCGTCGCGGCGACAGCA

Downstream 100 bases:

>100_bases
AAGCAGCCTCAGTAATAGAAGTTTCCACTAAAGTACCTAATTTTTCCCCTCACTACGGTTGCCACTACCTCTCCCCAGAG
GTTCATGCCCTCGAAGGGGC

Product: vinylacetyl-CoA Delta-isomerase

Products: NA

Alternate protein names: 4-BUDH; Gamma-aminobutyrate metabolism dehydratase/isomerase [H]

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MGLRKPEEYISALREKSKRAEIYVLGERVEDVTKHPFTLPAVKAFEWTYKAPWDPYFYDKEMGVHLAKAYSPFINEEINR
FNHIHQSPQDLAIKVKYFRKLSHKAGSCFQRCVGWDAINTISIISYDIDKWYEQKGEKPEHFEHFGTYYERFLKFLKYVQ
KEDIALAGVMTDAKGVRSLRPHEQPNKDVYVHVKEVADEGIIVRGAKANITGVVASEEMIVMPTRAMTEKDKDFAIAFAA
PIDAEGVTLVVGRYTNDTRRFEEPNVDALPGVFPAEAIVIFDDVFVPWERVFMFREYQFAAELVEIFSSYHRQAYGGCKP
GLADVIIGGAYHLAKQMGIANKCHIREKLTEMIFLTEAMHAGGLAAAWEGKKAASGTYYVDQMKANVTKQMVTRFPYEIA
RLAHDIAGGLIGTLPSYKDLMHEKIGKYLQQYLQCLPDYPAEWRHRVLRLLENITLSVEFLIESVHGAGSPEAQRIWMRR
FYPLEEMEDVALSLAGINPEEKPERKKQKEQT

Sequences:

>Translated_512_residues
MGLRKPEEYISALREKSKRAEIYVLGERVEDVTKHPFTLPAVKAFEWTYKAPWDPYFYDKEMGVHLAKAYSPFINEEINR
FNHIHQSPQDLAIKVKYFRKLSHKAGSCFQRCVGWDAINTISIISYDIDKWYEQKGEKPEHFEHFGTYYERFLKFLKYVQ
KEDIALAGVMTDAKGVRSLRPHEQPNKDVYVHVKEVADEGIIVRGAKANITGVVASEEMIVMPTRAMTEKDKDFAIAFAA
PIDAEGVTLVVGRYTNDTRRFEEPNVDALPGVFPAEAIVIFDDVFVPWERVFMFREYQFAAELVEIFSSYHRQAYGGCKP
GLADVIIGGAYHLAKQMGIANKCHIREKLTEMIFLTEAMHAGGLAAAWEGKKAASGTYYVDQMKANVTKQMVTRFPYEIA
RLAHDIAGGLIGTLPSYKDLMHEKIGKYLQQYLQCLPDYPAEWRHRVLRLLENITLSVEFLIESVHGAGSPEAQRIWMRR
FYPLEEMEDVALSLAGINPEEKPERKKQKEQT
>Mature_511_residues
GLRKPEEYISALREKSKRAEIYVLGERVEDVTKHPFTLPAVKAFEWTYKAPWDPYFYDKEMGVHLAKAYSPFINEEINRF
NHIHQSPQDLAIKVKYFRKLSHKAGSCFQRCVGWDAINTISIISYDIDKWYEQKGEKPEHFEHFGTYYERFLKFLKYVQK
EDIALAGVMTDAKGVRSLRPHEQPNKDVYVHVKEVADEGIIVRGAKANITGVVASEEMIVMPTRAMTEKDKDFAIAFAAP
IDAEGVTLVVGRYTNDTRRFEEPNVDALPGVFPAEAIVIFDDVFVPWERVFMFREYQFAAELVEIFSSYHRQAYGGCKPG
LADVIIGGAYHLAKQMGIANKCHIREKLTEMIFLTEAMHAGGLAAAWEGKKAASGTYYVDQMKANVTKQMVTRFPYEIAR
LAHDIAGGLIGTLPSYKDLMHEKIGKYLQQYLQCLPDYPAEWRHRVLRLLENITLSVEFLIESVHGAGSPEAQRIWMRRF
YPLEEMEDVALSLAGINPEEKPERKKQKEQT

Specific function: Catalyzes the reversible conversion of 4-hydroxybutyryl- CoA to crotonyl-CoA. The mechanism of the reaction seems to go through three steps:(1) the FAD-dependent oxidation of 4- hydroxybutyryl-CoA to 4-hydroxycrotonyl-CoA; (2) the hydroxyl group is substi

COG id: COG2368

COG function: function code Q; Aromatic ring hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR009100
- InterPro:   IPR004925 [H]

Pfam domain/function: PF03241 HpaB [H]

EC number: =4.2.1.120; =5.3.3.3 [H]

Molecular weight: Translated: 58714; Mature: 58583

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLRKPEEYISALREKSKRAEIYVLGERVEDVTKHPFTLPAVKAFEWTYKAPWDPYFYDK
CCCCCHHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCCCCCHHHEEECCCCCCCCCCCCH
EMGVHLAKAYSPFINEEINRFNHIHQSPQDLAIKVKYFRKLSHKAGSCFQRCVGWDAINT
HHCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCH
ISIISYDIDKWYEQKGEKPEHFEHFGTYYERFLKFLKYVQKEDIALAGVMTDAKGVRSLR
HHHEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHCHHHHHHCC
PHEQPNKDVYVHVKEVADEGIIVRGAKANITGVVASEEMIVMPTRAMTEKDKDFAIAFAA
CCCCCCCCEEEEEHHHCCCCEEEECCCCCEEEEEECCCEEEECCHHHCCCCCCEEEEEEC
PIDAEGVTLVVGRYTNDTRRFEEPNVDALPGVFPAEAIVIFDDVFVPWERVFMFREYQFA
CCCCCCEEEEEEECCCCHHHCCCCCCCCCCCCCCCCEEEEEECHHCCHHHHHHHHHHHHH
AELVEIFSSYHRQAYGGCKPGLADVIIGGAYHLAKQMGIANKCHIREKLTEMIFLTEAMH
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
AGGLAAAWEGKKAASGTYYVDQMKANVTKQMVTRFPYEIARLAHDIAGGLIGTLPSYKDL
CCCCEEECCCCCCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHH
MHEKIGKYLQQYLQCLPDYPAEWRHRVLRLLENITLSVEFLIESVHGAGSPEAQRIWMRR
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
FYPLEEMEDVALSLAGINPEEKPERKKQKEQT
CCCHHHHHHHHHHHCCCCCCCCHHHHHHCCCC
>Mature Secondary Structure 
GLRKPEEYISALREKSKRAEIYVLGERVEDVTKHPFTLPAVKAFEWTYKAPWDPYFYDK
CCCCHHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCCCCCHHHEEECCCCCCCCCCCCH
EMGVHLAKAYSPFINEEINRFNHIHQSPQDLAIKVKYFRKLSHKAGSCFQRCVGWDAINT
HHCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCH
ISIISYDIDKWYEQKGEKPEHFEHFGTYYERFLKFLKYVQKEDIALAGVMTDAKGVRSLR
HHHEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHCHHHHHHCC
PHEQPNKDVYVHVKEVADEGIIVRGAKANITGVVASEEMIVMPTRAMTEKDKDFAIAFAA
CCCCCCCCEEEEEHHHCCCCEEEECCCCCEEEEEECCCEEEECCHHHCCCCCCEEEEEEC
PIDAEGVTLVVGRYTNDTRRFEEPNVDALPGVFPAEAIVIFDDVFVPWERVFMFREYQFA
CCCCCCEEEEEEECCCCHHHCCCCCCCCCCCCCCCCEEEEEECHHCCHHHHHHHHHHHHH
AELVEIFSSYHRQAYGGCKPGLADVIIGGAYHLAKQMGIANKCHIREKLTEMIFLTEAMH
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
AGGLAAAWEGKKAASGTYYVDQMKANVTKQMVTRFPYEIARLAHDIAGGLIGTLPSYKDL
CCCCEEECCCCCCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHH
MHEKIGKYLQQYLQCLPDYPAEWRHRVLRLLENITLSVEFLIESVHGAGSPEAQRIWMRR
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
FYPLEEMEDVALSLAGINPEEKPERKKQKEQT
CCCHHHHHHHHHHHCCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11041350; 8344309 [H]