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The map label for this gene is cysK [H]

Identifier: 156937373

GI number: 156937373

Start: 521730

End: 522671

Strand: Direct

Name: cysK [H]

Synonym: Igni_0580

Alternate gene names: 156937373

Gene position: 521730-522671 (Clockwise)

Preceding gene: 156937371

Following gene: 156937375

Centisome position: 40.21

GC content: 58.39

Gene sequence:

>942_bases
ATGCCTATTGTGTGGGTGGGGGAGGCCGTAGGGAACACCCCAGCCGTGCTGCTGAGGAGGCTCACCAGCGGTAAGGGCAA
CGTAATGGTAAAGCTGGAATTTATGAATCCCAGTGGCAGCATAAAGGACAGGCCAGCTCTCTACATGATCAAAGAGGCGG
AGGAGAGGGGCCTCCTGAAACCGGGATCGACCATAGTCGAACCCTCTTCCGGAAACACAGCCCTCTCCTTGGCCATGCTC
GCAGCCGCCAAGGGCTACAAGATGGTCGCGGTGGTCCCGGAAACTACGAGCGAACAGAAGGTGAAAATGATGGAGTTGTT
GGGGGCCAAGGTAATTTTCTCCAAAGCGGGCGTACCCTTGGGCCACCCTGAACATCACTACACCTTGGCGAAGAGGTTGG
CTGAGGAGAACGGGTGGGTGATGCTGGATCAATACAAGAACGAGGCGAACGTGAGGGCCCATTACGAGACCACCGGGCCG
GAGGTACTCAAGCAAGCGAGGGAGCTCATGGGAGGGTTGGACGCCTTCGTGGCCGGGGTGGGCACCGGAGGCACCGTCAT
AGGCGTTGGAAAGTTCTTGAAGGAGAGGGTCAAGGGCGTCAAGGTAGTGGCAGTAGTTCCCAAGGGAACTAAAATAGCGG
GCTTCTTCGGCTCCGAGGGAGAGCGGCTGAGCCACGCCATAGAAGGCTTGACGGCGATGCCCGTCAGCGAGCTAATCAAG
AAGTACAAACACGTAATAGACGAAATAATCGAAGTGGACGACGAAGAGGCCGTGAAGACCGCTAGGGAGTTGGCCTCGAA
GGAGGGCCTCTTGGCAGGCTTGAGCGCCGGCGCAAACGTCCGCGCCGCACTGGAGCTGGAAAGGGAGGGCTTGAGGGTGG
CCACAGTCGCGCCGGACGGCGCCATTAGGTATTTGGAGAGGCTCACCTCACGAAGATCTTGA

Upstream 100 bases:

>100_bases
GGCTTTGCAGCCCAAGACGTCGGCGCCGTACAAGGGCTCCGCCTCTTCGGGCGCTTCAAGGTTCTCCTTATAATCTCCCC
TCGTCGCAGTGGGACGGCTG

Downstream 100 bases:

>100_bases
ACTCGCAGTGGGGGTCTCCCTTGGCCTCGCAAGCGACTTCCTTCACGTCGACCCTCCTTCCGGTAGCGCTCTCTAAGAGG
CCCGCCAGTATTCCCGCCAC

Product: pyridoxal-5'-phosphate-dependent enzyme, beta subunit

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; O-acetylserine sulfhydrylase [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MPIVWVGEAVGNTPAVLLRRLTSGKGNVMVKLEFMNPSGSIKDRPALYMIKEAEERGLLKPGSTIVEPSSGNTALSLAML
AAAKGYKMVAVVPETTSEQKVKMMELLGAKVIFSKAGVPLGHPEHHYTLAKRLAEENGWVMLDQYKNEANVRAHYETTGP
EVLKQARELMGGLDAFVAGVGTGGTVIGVGKFLKERVKGVKVVAVVPKGTKIAGFFGSEGERLSHAIEGLTAMPVSELIK
KYKHVIDEIIEVDDEEAVKTARELASKEGLLAGLSAGANVRAALELEREGLRVATVAPDGAIRYLERLTSRRS

Sequences:

>Translated_313_residues
MPIVWVGEAVGNTPAVLLRRLTSGKGNVMVKLEFMNPSGSIKDRPALYMIKEAEERGLLKPGSTIVEPSSGNTALSLAML
AAAKGYKMVAVVPETTSEQKVKMMELLGAKVIFSKAGVPLGHPEHHYTLAKRLAEENGWVMLDQYKNEANVRAHYETTGP
EVLKQARELMGGLDAFVAGVGTGGTVIGVGKFLKERVKGVKVVAVVPKGTKIAGFFGSEGERLSHAIEGLTAMPVSELIK
KYKHVIDEIIEVDDEEAVKTARELASKEGLLAGLSAGANVRAALELEREGLRVATVAPDGAIRYLERLTSRRS
>Mature_312_residues
PIVWVGEAVGNTPAVLLRRLTSGKGNVMVKLEFMNPSGSIKDRPALYMIKEAEERGLLKPGSTIVEPSSGNTALSLAMLA
AAKGYKMVAVVPETTSEQKVKMMELLGAKVIFSKAGVPLGHPEHHYTLAKRLAEENGWVMLDQYKNEANVRAHYETTGPE
VLKQARELMGGLDAFVAGVGTGGTVIGVGKFLKERVKGVKVVAVVPKGTKIAGFFGSEGERLSHAIEGLTAMPVSELIKK
YKHVIDEIIEVDDEEAVKTARELASKEGLLAGLSAGANVRAALELEREGLRVATVAPDGAIRYLERLTSRRS

Specific function: Two Cysteine Synthase Enzymes Are Found. Both Catalyze The Same Reaction. Cysteine Synthase B Can Also Use Thiosulfate In Place Of Sulfide To Give Cysteine Thiosulfonate As A Product. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=313, Percent_Identity=38.9776357827476, Blast_Score=211, Evalue=5e-55,
Organism=Homo sapiens, GI295821200, Length=313, Percent_Identity=38.9776357827476, Blast_Score=211, Evalue=5e-55,
Organism=Homo sapiens, GI4557415, Length=313, Percent_Identity=38.9776357827476, Blast_Score=211, Evalue=5e-55,
Organism=Homo sapiens, GI11345492, Length=297, Percent_Identity=27.6094276094276, Blast_Score=65, Evalue=1e-10,
Organism=Escherichia coli, GI2367138, Length=299, Percent_Identity=37.4581939799331, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1788754, Length=312, Percent_Identity=37.1794871794872, Blast_Score=155, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI32566674, Length=308, Percent_Identity=37.6623376623377, Blast_Score=181, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17562970, Length=309, Percent_Identity=37.5404530744337, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17535051, Length=312, Percent_Identity=35.8974358974359, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI115535073, Length=303, Percent_Identity=38.2838283828383, Blast_Score=167, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17561720, Length=306, Percent_Identity=37.9084967320261, Blast_Score=154, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI17534315, Length=318, Percent_Identity=35.8490566037736, Blast_Score=144, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI25147552, Length=318, Percent_Identity=36.1635220125786, Blast_Score=140, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI32566672, Length=222, Percent_Identity=34.2342342342342, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71996324, Length=318, Percent_Identity=27.0440251572327, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321594, Length=323, Percent_Identity=37.4613003095975, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6321449, Length=334, Percent_Identity=37.7245508982036, Blast_Score=169, Evalue=8e-43,
Organism=Drosophila melanogaster, GI24643623, Length=312, Percent_Identity=37.1794871794872, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI20129101, Length=312, Percent_Identity=37.1794871794872, Blast_Score=176, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 33584; Mature: 33452

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIVWVGEAVGNTPAVLLRRLTSGKGNVMVKLEFMNPSGSIKDRPALYMIKEAEERGLLK
CCEEEECCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCEEEEEEEHHHCCCCC
PGSTIVEPSSGNTALSLAMLAAAKGYKMVAVVPETTSEQKVKMMELLGAKVIFSKAGVPL
CCCEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCC
GHPEHHYTLAKRLAEENGWVMLDQYKNEANVRAHYETTGPEVLKQARELMGGLDAFVAGV
CCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCC
GTGGTVIGVGKFLKERVKGVKVVAVVPKGTKIAGFFGSEGERLSHAIEGLTAMPVSELIK
CCCCCEEHHHHHHHHHHCCEEEEEEECCCCEEEEEECCCCHHHHHHHCCCCCCCHHHHHH
KYKHVIDEIIEVDDEEAVKTARELASKEGLLAGLSAGANVRAALELEREGLRVATVAPDG
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEECCCCCCEEEEEEECCCCCEEEEECCCH
AIRYLERLTSRRS
HHHHHHHHHHCCC
>Mature Secondary Structure 
PIVWVGEAVGNTPAVLLRRLTSGKGNVMVKLEFMNPSGSIKDRPALYMIKEAEERGLLK
CEEEECCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCEEEEEEEHHHCCCCC
PGSTIVEPSSGNTALSLAMLAAAKGYKMVAVVPETTSEQKVKMMELLGAKVIFSKAGVPL
CCCEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCC
GHPEHHYTLAKRLAEENGWVMLDQYKNEANVRAHYETTGPEVLKQARELMGGLDAFVAGV
CCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCC
GTGGTVIGVGKFLKERVKGVKVVAVVPKGTKIAGFFGSEGERLSHAIEGLTAMPVSELIK
CCCCCEEHHHHHHHHHHCCEEEEEEECCCCEEEEEECCCCHHHHHHHCCCCCCCHHHHHH
KYKHVIDEIIEVDDEEAVKTARELASKEGLLAGLSAGANVRAALELEREGLRVATVAPDG
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEECCCCCCEEEEEEECCCCCEEEEECCCH
AIRYLERLTSRRS
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA