Click here to switch to the map view.

The map label for this gene is tgtA [H]

Identifier: 156937272

GI number: 156937272

Start: 424991

End: 426523

Strand: Direct

Name: tgtA [H]

Synonym: Igni_0478

Alternate gene names: 156937272

Gene position: 424991-426523 (Clockwise)

Preceding gene: 156937271

Following gene: 156937275

Centisome position: 32.75

GC content: 63.27

Gene sequence:

>1533_bases
TTGATCTGGGAGCCGGAGGCCAGGGACGGGCCGGCGAGGGCCGGGAAGCTCAAGGTAGGCGCCTACGAGGTCGAGACCCC
CGCCCTTTTGGCGGTGGTGGACCCGGACCCGAAGAAGCAACTCGTCCCCTTGGACGAGATGAGGAGGGCCGGGGTCCAAG
TAATAATGACCTCAGCCTTCATAGCTAAGAAGAAGGTAGGCCCAACGAACTTGAAGGAGCGCTTGGGGTGGGAGGGCCTC
CTATACACCGACTCGGGGACCTTTCAAGCGTACAGCAGGGGGGTGAGGGTGGACCCGGAGGAGTCGGTGAGGTACCAGAT
AAGCGCCGGCTCCGACATAATAACGCCCGTGGACCTCTTCAGCTTGCCCACGGACAGCAAGGAGGTAGCGGCGAAGAAGG
CGGAGGTGAGCTTCCGGAGGTGGCTGAGGGCGAGGGAGCTGAAGGAGGAGGTGAGCGCCCCGGTACAAGGGGGCCTCTAC
CCGGACGTGAGGGCGGCCGTCGCGAGGAGGTACTCCGAGGCCGGGGCCAGGCTCTTGGCCGTGGGGGGTATAGTTCCCTT
GATGGAAGAGTACAAGTTCAAGGAGTTGGTTAACGCGGTGCTCCCGGTCCTCGCCTCCAGGCCGCCGGAGGCCGCGGTGC
ACGCCTTCGGGGCCGGCCACCCGCTCGCCTTCCCCCTCTTGGCCTTCCTAGGCGTGGACCTCTTCGACTCGGCCATGTAC
GCTATAGCGGCGAGGGAGGGTAGGTACTTAACGCCGTTTGGTACCTTTAGGCTCGAAGAATTAGTAATGATGAGGGAGTT
CCCCTGCGACTGCCCCGCTTGCTCCTCCCTCTCCCCGCGCGACTTGCTGTCCATGAGCGAGGAGGAGAGGACCAAGTTCT
TAGCCCTTCACAACCTTTACGCAGCAATTAAAATGGTGAAGGAAATAAGGGAGAGGATAGTTTACGGCACCTTCCACAAG
TGGGCGATAGCCTTCGCCCACAGCCACCCGAGGCTGTACGAGGCCTTTGCAGAGGCCTTGGGCAAGTGGAGGGGCTACTT
CGAGAGGGAGAGGAACGCCCTCCCCAAGTCGCCGGTCCCGGAGGGGTGCGAGCTCTGCGACTCCAGCCCGGAGAGGGACC
CGGTGGGGGAGGGGACTTACGGGACGAGGGAGCTGTTCAACGTCATCGCCAAGAGGGCCTACGGGAAGGGGCTGAGTTCC
GAAGAACCCTTGGAGGCCTTAAGGCCCCCTAGGGCCAGCTTCTCGCCCCGCGCGCTGAGGGAGCTCTGCTCGGTGACCCT
CAACTCCCCTCCCAAGGGGAGGGCGATAAGGAAGAAGGACTTAAAGGAAGTTAACTGCTTGTTGAGGCCCAACCATGAGG
TCCTCGTCCTCTGGGAGGGAGGGGAGGCGAAGGCCGTTTCCCTCGTCTCGTTCGCGGAGCTCTCGTTGGCCTCCGAAGAG
GCCGTGGCGCTCTTACTCCCCCCTGAGGGCCTCCACGGGGTCAAGCTTGGAGGCCTTGTAGGCCGGGTAGAGGCCGGCGA
CCACGGCGGTTAA

Upstream 100 bases:

>100_bases
CGTGTTGACTTACGAGCCTTTCTCCCGGTAGCCCCTCGGGAGAGGGGCCCGGGGTTATAGACAAGAGGAAACTCTGAGAC
TGCCCGGGCCCGGGAAGAGC

Downstream 100 bases:

>100_bases
CACGGAGAAGAGCGCCGCCTCAGCGACCAGCTGGGGGCTCACGTAAGCCTTGGCGGTCTTCAAGAACTCCGCTTGTATGT
AGCTCTTCAGCTGGGCCCCT

Product: tRNA-guanine transglycosylase, various specificities

Products: tRNAqueuine; guanine [C]

Alternate protein names: Archaeal tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MIWEPEARDGPARAGKLKVGAYEVETPALLAVVDPDPKKQLVPLDEMRRAGVQVIMTSAFIAKKKVGPTNLKERLGWEGL
LYTDSGTFQAYSRGVRVDPEESVRYQISAGSDIITPVDLFSLPTDSKEVAAKKAEVSFRRWLRARELKEEVSAPVQGGLY
PDVRAAVARRYSEAGARLLAVGGIVPLMEEYKFKELVNAVLPVLASRPPEAAVHAFGAGHPLAFPLLAFLGVDLFDSAMY
AIAAREGRYLTPFGTFRLEELVMMREFPCDCPACSSLSPRDLLSMSEEERTKFLALHNLYAAIKMVKEIRERIVYGTFHK
WAIAFAHSHPRLYEAFAEALGKWRGYFERERNALPKSPVPEGCELCDSSPERDPVGEGTYGTRELFNVIAKRAYGKGLSS
EEPLEALRPPRASFSPRALRELCSVTLNSPPKGRAIRKKDLKEVNCLLRPNHEVLVLWEGGEAKAVSLVSFAELSLASEE
AVALLLPPEGLHGVKLGGLVGRVEAGDHGG

Sequences:

>Translated_510_residues
MIWEPEARDGPARAGKLKVGAYEVETPALLAVVDPDPKKQLVPLDEMRRAGVQVIMTSAFIAKKKVGPTNLKERLGWEGL
LYTDSGTFQAYSRGVRVDPEESVRYQISAGSDIITPVDLFSLPTDSKEVAAKKAEVSFRRWLRARELKEEVSAPVQGGLY
PDVRAAVARRYSEAGARLLAVGGIVPLMEEYKFKELVNAVLPVLASRPPEAAVHAFGAGHPLAFPLLAFLGVDLFDSAMY
AIAAREGRYLTPFGTFRLEELVMMREFPCDCPACSSLSPRDLLSMSEEERTKFLALHNLYAAIKMVKEIRERIVYGTFHK
WAIAFAHSHPRLYEAFAEALGKWRGYFERERNALPKSPVPEGCELCDSSPERDPVGEGTYGTRELFNVIAKRAYGKGLSS
EEPLEALRPPRASFSPRALRELCSVTLNSPPKGRAIRKKDLKEVNCLLRPNHEVLVLWEGGEAKAVSLVSFAELSLASEE
AVALLLPPEGLHGVKLGGLVGRVEAGDHGG
>Mature_510_residues
MIWEPEARDGPARAGKLKVGAYEVETPALLAVVDPDPKKQLVPLDEMRRAGVQVIMTSAFIAKKKVGPTNLKERLGWEGL
LYTDSGTFQAYSRGVRVDPEESVRYQISAGSDIITPVDLFSLPTDSKEVAAKKAEVSFRRWLRARELKEEVSAPVQGGLY
PDVRAAVARRYSEAGARLLAVGGIVPLMEEYKFKELVNAVLPVLASRPPEAAVHAFGAGHPLAFPLLAFLGVDLFDSAMY
AIAAREGRYLTPFGTFRLEELVMMREFPCDCPACSSLSPRDLLSMSEEERTKFLALHNLYAAIKMVKEIRERIVYGTFHK
WAIAFAHSHPRLYEAFAEALGKWRGYFERERNALPKSPVPEGCELCDSSPERDPVGEGTYGTRELFNVIAKRAYGKGLSS
EEPLEALRPPRASFSPRALRELCSVTLNSPPKGRAIRKKDLKEVNCLLRPNHEVLVLWEGGEAKAVSLVSFAELSLASEE
AVALLLPPEGLHGVKLGGLVGRVEAGDHGG

Specific function: Exchanges the guanine residue with 7-cyano-7- deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D- loop) of archaeal tRNAs [H]

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=349, Percent_Identity=25.7879656160458, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1786607, Length=371, Percent_Identity=24.2587601078167, Blast_Score=95, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17544680, Length=368, Percent_Identity=22.554347826087, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI19920510, Length=357, Percent_Identity=24.0896358543417, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004804
- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR002616
- InterPro:   IPR004521 [H]

Pfam domain/function: PF01472 PUA; PF01702 TGT [H]

EC number: 2.4.2.29 [C]

Molecular weight: Translated: 56106; Mature: 56106

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIWEPEARDGPARAGKLKVGAYEVETPALLAVVDPDPKKQLVPLDEMRRAGVQVIMTSAF
CCCCCCCCCCCCCCCEEEECEEEECCCEEEEEECCCCCCCCCCHHHHHHCCHHHHHHHHH
IAKKKVGPTNLKERLGWEGLLYTDSGTFQAYSRGVRVDPEESVRYQISAGSDIITPVDLF
HHHCCCCCCCHHHHCCCCEEEEECCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCCHHHH
SLPTDSKEVAAKKAEVSFRRWLRARELKEEVSAPVQGGLYPDVRAAVARRYSEAGARLLA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCEEEE
VGGIVPLMEEYKFKELVNAVLPVLASRPPEAAVHAFGAGHPLAFPLLAFLGVDLFDSAMY
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
AIAAREGRYLTPFGTFRLEELVMMREFPCDCPACSSLSPRDLLSMSEEERTKFLALHNLY
HHHHCCCCEECCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHH
AAIKMVKEIRERIVYGTFHKWAIAFAHSHPRLYEAFAEALGKWRGYFERERNALPKSPVP
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EGCELCDSSPERDPVGEGTYGTRELFNVIAKRAYGKGLSSEEPLEALRPPRASFSPRALR
CHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCHHHHH
ELCSVTLNSPPKGRAIRKKDLKEVNCLLRPNHEVLVLWEGGEAKAVSLVSFAELSLASEE
HHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEHHHHHHHHHHCCCC
AVALLLPPEGLHGVKLGGLVGRVEAGDHGG
CEEEEECCCCCCCEEECCEEEEECCCCCCC
>Mature Secondary Structure
MIWEPEARDGPARAGKLKVGAYEVETPALLAVVDPDPKKQLVPLDEMRRAGVQVIMTSAF
CCCCCCCCCCCCCCCEEEECEEEECCCEEEEEECCCCCCCCCCHHHHHHCCHHHHHHHHH
IAKKKVGPTNLKERLGWEGLLYTDSGTFQAYSRGVRVDPEESVRYQISAGSDIITPVDLF
HHHCCCCCCCHHHHCCCCEEEEECCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCCHHHH
SLPTDSKEVAAKKAEVSFRRWLRARELKEEVSAPVQGGLYPDVRAAVARRYSEAGARLLA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCEEEE
VGGIVPLMEEYKFKELVNAVLPVLASRPPEAAVHAFGAGHPLAFPLLAFLGVDLFDSAMY
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
AIAAREGRYLTPFGTFRLEELVMMREFPCDCPACSSLSPRDLLSMSEEERTKFLALHNLY
HHHHCCCCEECCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHH
AAIKMVKEIRERIVYGTFHKWAIAFAHSHPRLYEAFAEALGKWRGYFERERNALPKSPVP
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EGCELCDSSPERDPVGEGTYGTRELFNVIAKRAYGKGLSSEEPLEALRPPRASFSPRALR
CHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCHHHHH
ELCSVTLNSPPKGRAIRKKDLKEVNCLLRPNHEVLVLWEGGEAKAVSLVSFAELSLASEE
HHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEHHHHHHHHHHCCCC
AVALLLPPEGLHGVKLGGLVGRVEAGDHGG
CEEEEECCCCCCCEEECCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Zn; Mg [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.000014 {7-(aminomethyl)-7-deazaguanine}} 0.00006 {guanine}} 0.000053 {guanine}} [C]

Substrates: tRNAguanine; queuine [C]

Specific reaction: tRNAguanine + queuine = tRNAqueuine + guanine [C]

General reaction: Pentosyl group transfer [C]

Inhibitor: Bisimidoester; Dimethyl suberimidate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]