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The map label for this gene is panB

Identifier: 156937186

GI number: 156937186

Start: 345247

End: 346038

Strand: Direct

Name: panB

Synonym: Igni_0392

Alternate gene names: 156937186

Gene position: 345247-346038 (Clockwise)

Preceding gene: 156937184

Following gene: 156937187

Centisome position: 26.61

GC content: 58.21

Gene sequence:

>792_bases
GTGAAGAAGGTTACCATAAGAGATATTATGAAAAAGTACAAAAATGGCGAAAAGATCGTCATGGTTACCGCTTACGATTA
CCCGTTCGCCAAGCTCGTGGACGAGGCCGGCGTGGACATGATCCTGGTTGGGGACTCCTTGGGCATGGTAGTCCTCGGCT
ACCCCTCTACCAACCAAGTCACCATGAAGGACATGCTCCACCACGTGGCGGCGGTCGCGAGGGCGAACCCCAAAGCGATG
ATAGTTGGGGACATGCCCTTCGGGAGCTACGAGGTTTCCGAGGAAGAGGCGGTGAGAAACGCGGTGGAGCTGGTGAGGGC
CGGGGCTGAAGCGGTGAAGTTAGAAGGGGGAAAGGAGGTGGCGGACAAGGTTGAGGCCATAGTGAAGGCCGGGATACCGG
TGATGGGTCACTTGGGCTTGACGCCGCAGAAGAGGCACGCCTTGGGCGGCTACCGCTTGAGGGGCAAGACGGAAGAGGAA
GCGAGGGAGCTGCTGGAAGACGCGAAGGCGTTGGAGGAGGCGGGGGTCTTCTCCATAGTCCTTGAATTCGTCAAGGCCGA
GGTGGCTAAGAAGATCACCGAAGAGGTCGGCGTGCCTACGATATGCATAGGCGCCGGTCCTTGGTGTTCGGGCCAAGTGT
TGGTAATTCACGACATATTGGGCTTGGCCCCCTTCAGCCCTCCCTTCGCTAAGAAGTACTTCGACTGCGGCAAGGCCATA
GTGGAAGCTGTGAAGAAGTTCGCCGAGGAGGTCAGGTCGGGAGAGTTCCCGGGGGAGGGGTACTACTGGTAG

Upstream 100 bases:

>100_bases
CAGCTTCGTCCACAAGCCCCCTTACCTAGGCCCGGCCGCCCGGGGCCCTTATTGTGTGAACAGAAATAACCGCTGGATGT
AAGCCCTCTGGGCTAAGGAA

Downstream 100 bases:

>100_bases
TTTCCTAAGCCTCCACTTGATAACTCCCCTTAGGGGGAGGGCTACCGGTCACGGATTATGTCTGCCAACATACCTCTGAA
GAGGGTCGGCACCTACGAAT

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MKKVTIRDIMKKYKNGEKIVMVTAYDYPFAKLVDEAGVDMILVGDSLGMVVLGYPSTNQVTMKDMLHHVAAVARANPKAM
IVGDMPFGSYEVSEEEAVRNAVELVRAGAEAVKLEGGKEVADKVEAIVKAGIPVMGHLGLTPQKRHALGGYRLRGKTEEE
ARELLEDAKALEEAGVFSIVLEFVKAEVAKKITEEVGVPTICIGAGPWCSGQVLVIHDILGLAPFSPPFAKKYFDCGKAI
VEAVKKFAEEVRSGEFPGEGYYW

Sequences:

>Translated_263_residues
MKKVTIRDIMKKYKNGEKIVMVTAYDYPFAKLVDEAGVDMILVGDSLGMVVLGYPSTNQVTMKDMLHHVAAVARANPKAM
IVGDMPFGSYEVSEEEAVRNAVELVRAGAEAVKLEGGKEVADKVEAIVKAGIPVMGHLGLTPQKRHALGGYRLRGKTEEE
ARELLEDAKALEEAGVFSIVLEFVKAEVAKKITEEVGVPTICIGAGPWCSGQVLVIHDILGLAPFSPPFAKKYFDCGKAI
VEAVKKFAEEVRSGEFPGEGYYW
>Mature_263_residues
MKKVTIRDIMKKYKNGEKIVMVTAYDYPFAKLVDEAGVDMILVGDSLGMVVLGYPSTNQVTMKDMLHHVAAVARANPKAM
IVGDMPFGSYEVSEEEAVRNAVELVRAGAEAVKLEGGKEVADKVEAIVKAGIPVMGHLGLTPQKRHALGGYRLRGKTEEE
ARELLEDAKALEEAGVFSIVLEFVKAEVAKKITEEVGVPTICIGAGPWCSGQVLVIHDILGLAPFSPPFAKKYFDCGKAI
VEAVKKFAEEVRSGEFPGEGYYW

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=265, Percent_Identity=44.5283018867925, Blast_Score=219, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6319653, Length=267, Percent_Identity=41.1985018726592, Blast_Score=204, Evalue=1e-53,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_IGNH4 (A8A9H3)

Other databases:

- EMBL:   CP000816
- RefSeq:   YP_001434982.1
- ProteinModelPortal:   A8A9H3
- SMR:   A8A9H3
- STRING:   A8A9H3
- GeneID:   5562368
- GenomeReviews:   CP000816_GR
- KEGG:   iho:Igni_0392
- eggNOG:   arNOG04418
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- ProtClustDB:   PRK00311
- BioCyc:   IHOS453591:IGNI_0392-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 28575; Mature: 28575

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: ACT_SITE 182-182 BINDING 84-84 BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVTIRDIMKKYKNGEKIVMVTAYDYPFAKLVDEAGVDMILVGDSLGMVVLGYPSTNQV
CCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHCCCCEEEECCCCCEEEEECCCCCCE
TMKDMLHHVAAVARANPKAMIVGDMPFGSYEVSEEEAVRNAVELVRAGAEAVKLEGGKEV
EHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHEEECCCHHH
ADKVEAIVKAGIPVMGHLGLTPQKRHALGGYRLRGKTEEEARELLEDAKALEEAGVFSIV
HHHHHHHHHHCCCCEECCCCCCCHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
LEFVKAEVAKKITEEVGVPTICIGAGPWCSGQVLVIHDILGLAPFSPPFAKKYFDCGKAI
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEHHHHHHHCCCCCCHHHHHHHHHHHHH
VEAVKKFAEEVRSGEFPGEGYYW
HHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKKVTIRDIMKKYKNGEKIVMVTAYDYPFAKLVDEAGVDMILVGDSLGMVVLGYPSTNQV
CCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHCCCCEEEECCCCCEEEEECCCCCCE
TMKDMLHHVAAVARANPKAMIVGDMPFGSYEVSEEEAVRNAVELVRAGAEAVKLEGGKEV
EHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHEEECCCHHH
ADKVEAIVKAGIPVMGHLGLTPQKRHALGGYRLRGKTEEEARELLEDAKALEEAGVFSIV
HHHHHHHHHHCCCCEECCCCCCCHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
LEFVKAEVAKKITEEVGVPTICIGAGPWCSGQVLVIHDILGLAPFSPPFAKKYFDCGKAI
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEHHHHHHHCCCCCCHHHHHHHHHHHHH
VEAVKKFAEEVRSGEFPGEGYYW
HHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA