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The map label for this gene is proS

Identifier: 156937156

GI number: 156937156

Start: 315677

End: 317113

Strand: Direct

Name: proS

Synonym: Igni_0362

Alternate gene names: 156937156

Gene position: 315677-317113 (Clockwise)

Preceding gene: 156937155

Following gene: 156937160

Centisome position: 24.33

GC content: 57.69

Gene sequence:

>1437_bases
GTGAAGCTTAACAAGGAGGAGAACTTCAGCGAGTGGTTCGATACGGTCCTAAGGGAGAGCGGGCTCTACGACTACGGCCG
TTACCCGGTTAAGGGCATGGGGGTGTGGCCCCCCTTCGGCTTCAAGCTCAGGAAGTTGGTCCTAAACATAATAAGGGATC
TCTTGGACTCCACCGGTCACGAGGAAGTGTTGTTCCCGGTCCTAATTCCCAAGACGTTATTGGAGAAAGAGAGCGAACAC
ATAAGGGGCTTCGAGGGCGAGGTCTTCTGGGTGACCAAGGGAGGACACGAGGACTTGGACGTCCCCTTAGCTCTGAGGCC
CACGAGCGAGACCGCTATAAGCTACATGGAGTCCTTCTGGATAAGCAGCTACAAGCAACTCCCCATGAAGCTTTACCAAA
TAGTCCCGGTGTATAGGTACGAGACCAAGGCTACGAGGCCGCTGATAAGGCTGAGGGAGGTGAGCACCTTCAAGGAGGCC
CACACAGCTCACGAGAGCTTCGAGGGGGCTGACTCTCAGTGCGCCGAGGCGATAGAGATCTACAAGAAGGTCTTCGATAG
GCTGGGAATACCGTATATGATAAGCCAGAGGCCCCCTTGGGACAAGTTCGCGGGGGCCCTCTACACGGTGGCCTTCGACA
CCGTTATGCCCGACGGGAGGGTGCTCCAGATAGGCACGGTCCACCACTTAGGCCAAAACTTCTCCGTGCCCTTCGAGGTC
AGGTTCCACACGGAGGACGGGGACAAGGACTACGTCTGGCAGACCTCCTACGGGCTGTCGGACAGAGTGATAGCGTCGTT
GGTGGCCGTGCACGGGGACGAGAGGGGCTTGGTCCTCCCTCCGGAGGTCGCCCCCGTACAAGTGGTGATAGTTCCCATAC
CTCAGAAGGAGGAGGAACAACAGAGGAAGGTCTTGGAGGAAGCCAAGAGGGTGGAGGAGGAGCTCAAGGCTCGGGGCTGG
AGGACGGTGCTCGACGACAGGGACGAGCTGACTCCCGGAGCGAAGTACTACGAGTGGGAACTCAAGGGAGTCCCATTTAG
GATAGAGATAGGTAAGAAGGAGGTTGAGGGGGACGAGCTCACTGTGGCGCGCAGGGACTTGAAGAAGAGGGTGAAAGTGA
AGAAAGGTGAGATCCACCAGAGGTTAAAGGAAATGAGCGACGACATGCTCAACAACATGAGGGAGAGGGCGTGGAGCTTC
ATGAAGAGTAGGATAAAGAGGGTGAGGAGCTTGGAGGAGGCGAAGGAGTTAGTGGAGAAGAGGTACGTGGTGGAGTTGCC
GTGGTGCGGCTCCAAGGAGTGTGGGCTGAGGGTGGACGAGGAGGTGGGGAGGGTGTTAGGGGTGCCGCTGGACGAGGATG
CGGAGGCGAAGGGCGAGAGGTGCGCGGTCTGCGGGAGGGAGGCGAAGTGGTGGATAAGGGTAGCAAAAACCTATTAG

Upstream 100 bases:

>100_bases
GTAAAGGTCTCCAGGGGGAGGTACAGCCCTAGGGAGGAGCTCTTCTGGTGTCCCGACAACCAATAAAATTTTTAGCACTA
CCTCGGGCTGGGAGGGGCGC

Downstream 100 bases:

>100_bases
TCGTGCGCTTCTTCGGCCCCACTCAACATTTCGGAGTGCTTTACCTTCGGGACGGGCAAGTCCTTGACGGGCTTGAACCT
GTCCTTGAGCCTCTCTAGCA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MKLNKEENFSEWFDTVLRESGLYDYGRYPVKGMGVWPPFGFKLRKLVLNIIRDLLDSTGHEEVLFPVLIPKTLLEKESEH
IRGFEGEVFWVTKGGHEDLDVPLALRPTSETAISYMESFWISSYKQLPMKLYQIVPVYRYETKATRPLIRLREVSTFKEA
HTAHESFEGADSQCAEAIEIYKKVFDRLGIPYMISQRPPWDKFAGALYTVAFDTVMPDGRVLQIGTVHHLGQNFSVPFEV
RFHTEDGDKDYVWQTSYGLSDRVIASLVAVHGDERGLVLPPEVAPVQVVIVPIPQKEEEQQRKVLEEAKRVEEELKARGW
RTVLDDRDELTPGAKYYEWELKGVPFRIEIGKKEVEGDELTVARRDLKKRVKVKKGEIHQRLKEMSDDMLNNMRERAWSF
MKSRIKRVRSLEEAKELVEKRYVVELPWCGSKECGLRVDEEVGRVLGVPLDEDAEAKGERCAVCGREAKWWIRVAKTY

Sequences:

>Translated_478_residues
MKLNKEENFSEWFDTVLRESGLYDYGRYPVKGMGVWPPFGFKLRKLVLNIIRDLLDSTGHEEVLFPVLIPKTLLEKESEH
IRGFEGEVFWVTKGGHEDLDVPLALRPTSETAISYMESFWISSYKQLPMKLYQIVPVYRYETKATRPLIRLREVSTFKEA
HTAHESFEGADSQCAEAIEIYKKVFDRLGIPYMISQRPPWDKFAGALYTVAFDTVMPDGRVLQIGTVHHLGQNFSVPFEV
RFHTEDGDKDYVWQTSYGLSDRVIASLVAVHGDERGLVLPPEVAPVQVVIVPIPQKEEEQQRKVLEEAKRVEEELKARGW
RTVLDDRDELTPGAKYYEWELKGVPFRIEIGKKEVEGDELTVARRDLKKRVKVKKGEIHQRLKEMSDDMLNNMRERAWSF
MKSRIKRVRSLEEAKELVEKRYVVELPWCGSKECGLRVDEEVGRVLGVPLDEDAEAKGERCAVCGREAKWWIRVAKTY
>Mature_478_residues
MKLNKEENFSEWFDTVLRESGLYDYGRYPVKGMGVWPPFGFKLRKLVLNIIRDLLDSTGHEEVLFPVLIPKTLLEKESEH
IRGFEGEVFWVTKGGHEDLDVPLALRPTSETAISYMESFWISSYKQLPMKLYQIVPVYRYETKATRPLIRLREVSTFKEA
HTAHESFEGADSQCAEAIEIYKKVFDRLGIPYMISQRPPWDKFAGALYTVAFDTVMPDGRVLQIGTVHHLGQNFSVPFEV
RFHTEDGDKDYVWQTSYGLSDRVIASLVAVHGDERGLVLPPEVAPVQVVIVPIPQKEEEQQRKVLEEAKRVEEELKARGW
RTVLDDRDELTPGAKYYEWELKGVPFRIEIGKKEVEGDELTVARRDLKKRVKVKKGEIHQRLKEMSDDMLNNMRERAWSF
MKSRIKRVRSLEEAKELVEKRYVVELPWCGSKECGLRVDEEVGRVLGVPLDEDAEAKGERCAVCGREAKWWIRVAKTY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily

Homologues:

Organism=Homo sapiens, GI62241042, Length=501, Percent_Identity=31.1377245508982, Blast_Score=272, Evalue=5e-73,
Organism=Escherichia coli, GI1786392, Length=174, Percent_Identity=31.0344827586207, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71991342, Length=491, Percent_Identity=31.5682281059063, Blast_Score=270, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI71991346, Length=361, Percent_Identity=35.7340720221607, Blast_Score=239, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6321808, Length=505, Percent_Identity=32.2772277227723, Blast_Score=279, Evalue=7e-76,
Organism=Drosophila melanogaster, GI24649468, Length=501, Percent_Identity=31.3373253493014, Blast_Score=272, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24649466, Length=501, Percent_Identity=31.3373253493014, Blast_Score=272, Evalue=4e-73,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_IGNH4 (A8A9E3)

Other databases:

- EMBL:   CP000816
- RefSeq:   YP_001434952.1
- ProteinModelPortal:   A8A9E3
- SMR:   A8A9E3
- STRING:   A8A9E3
- GeneID:   5561887
- GenomeReviews:   CP000816_GR
- KEGG:   iho:Igni_0362
- eggNOG:   arNOG04466
- HOGENOM:   HBG334108
- OMA:   GIPYPEE
- ProtClustDB:   PRK08661
- BioCyc:   IHOS453591:IGNI_0362-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01571
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.30.110.30
- PANTHER:   PTHR11451:SF6
- PRINTS:   PR01046
- SMART:   SM00946
- TIGRFAMs:   TIGR00408

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd; SSF64586 Pro-tRNA_synth_II_C

EC number: =6.1.1.15

Molecular weight: Translated: 55399; Mature: 55399

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNKEENFSEWFDTVLRESGLYDYGRYPVKGMGVWPPFGFKLRKLVLNIIRDLLDSTGH
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
EEVLFPVLIPKTLLEKESEHIRGFEGEVFWVTKGGHEDLDVPLALRPTSETAISYMESFW
CCEEEHHHHHHHHHHCCHHHHCCCCCEEEEEECCCCCCCCCCEEECCCHHHHHHHHHHHH
ISSYKQLPMKLYQIVPVYRYETKATRPLIRLREVSTFKEAHTAHESFEGADSQCAEAIEI
HHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
YKKVFDRLGIPYMISQRPPWDKFAGALYTVAFDTVMPDGRVLQIGTVHHLGQNFSVPFEV
HHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECHHHCCCCCCCCEEE
RFHTEDGDKDYVWQTSYGLSDRVIASLVAVHGDERGLVLPPEVAPVQVVIVPIPQKEEEQ
EEECCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEEECCCCHHHH
QRKVLEEAKRVEEELKARGWRTVLDDRDELTPGAKYYEWELKGVPFRIEIGKKEVEGDEL
HHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCHH
TVARRDLKKRVKVKKGEIHQRLKEMSDDMLNNMRERAWSFMKSRIKRVRSLEEAKELVEK
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYVVELPWCGSKECGLRVDEEVGRVLGVPLDEDAEAKGERCAVCGREAKWWIRVAKTY
CCEEEECCCCCCCCCCEECHHHHHHCCCCCCCCCCCCCCEEEECCCCCEEEEEEEECC
>Mature Secondary Structure
MKLNKEENFSEWFDTVLRESGLYDYGRYPVKGMGVWPPFGFKLRKLVLNIIRDLLDSTGH
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
EEVLFPVLIPKTLLEKESEHIRGFEGEVFWVTKGGHEDLDVPLALRPTSETAISYMESFW
CCEEEHHHHHHHHHHCCHHHHCCCCCEEEEEECCCCCCCCCCEEECCCHHHHHHHHHHHH
ISSYKQLPMKLYQIVPVYRYETKATRPLIRLREVSTFKEAHTAHESFEGADSQCAEAIEI
HHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
YKKVFDRLGIPYMISQRPPWDKFAGALYTVAFDTVMPDGRVLQIGTVHHLGQNFSVPFEV
HHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECHHHCCCCCCCCEEE
RFHTEDGDKDYVWQTSYGLSDRVIASLVAVHGDERGLVLPPEVAPVQVVIVPIPQKEEEQ
EEECCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEEECCCCHHHH
QRKVLEEAKRVEEELKARGWRTVLDDRDELTPGAKYYEWELKGVPFRIEIGKKEVEGDEL
HHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCHH
TVARRDLKKRVKVKKGEIHQRLKEMSDDMLNNMRERAWSFMKSRIKRVRSLEEAKELVEK
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYVVELPWCGSKECGLRVDEEVGRVLGVPLDEDAEAKGERCAVCGREAKWWIRVAKTY
CCEEEECCCCCCCCCCEECHHHHHHCCCCCCCCCCCCCCEEEECCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA