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The map label for this gene is lysX [H]

Identifier: 156936852

GI number: 156936852

Start: 54109

End: 54954

Strand: Direct

Name: lysX [H]

Synonym: Igni_0057

Alternate gene names: 156936852

Gene position: 54109-54954 (Clockwise)

Preceding gene: 156936850

Following gene: 156936853

Centisome position: 4.17

GC content: 52.01

Gene sequence:

>846_bases
ATGAAGGTGGGGATAGCCTACGACCATCCCAGGTGGGAGGAGAAACACATAATCGAGCTGTTGAGGTCTAGGGGCCACGA
GGTGATAGAGTATCCTCTCAGACATAGTTCGTTCGAAATAGGCGTCTCCAGCGAACTTCCGGACGTGGTGGTTCAAAGGG
CTGTGAGCAGCGCTAGGGCGATTTCCTTCACCGCGCACATGGAGTCAATGGGCGTTCCGGTAGTTAATTCTCTTCATACA
CAGCTGGTGTGCGATAATAAGGTGTTGACAGACTCGGCCCTCAATAAGGAGAACGTGCCTCGGCCCAGAACGTTCATCGC
GTACGACTTGGAGAGCGCCTTAGAGGCCGCTAGGGAGTTAGGCTTTCCCCTAGTCGTTAAGCCCCTACAGGGGAGTTGGG
GGAGGCTTCAAGCCTTAGTAAAGGACGAGGACGCCCTAAAGGCCATAATAGAGCATAGGGAAGCCATGCCTTCGCCCCAG
TTCAAGGTCCATTACCTTCAAGAATATATAAACAAGCCTAATAGAGACATCAGAGTGTTCGTAGTAGGGGACTCCGTTCC
AGTAGCTATATATAGAATCTCCGAGAGGGAGTGGAGGACCAACACGGCCCTTGGGGGCAGGGCCGAGAAGGCAGAAGTCG
ACGAGGAGCTCGAGGAACTTGCCATAAAGGCGGCTAGGGCTGTGGGAGGCGGCGTGTTGGGCGTGGACGTGGTCGAAGAT
CCAGAGAGGGGCTACTTAGTGATTGAGGTTAATTCCAACGTGGACTTTAAAAACACATACAAGGTTACAGGCTTTGATAT
GGGAGAAGCCATAATAGACTATGCTTTGTCTTTAGTAAAACGTTGA

Upstream 100 bases:

>100_bases
AAAGCCGATGAGGACCCGTGTTTCAGCCGAACTCGTGAGGAGACGAGGCCGTGCACCGAGGCTTAAAGAGTAAATGGTCA
TTCGAAGTGGCGGATGAAGA

Downstream 100 bases:

>100_bases
GGTCCTTAAATAGGCCACTTAACTGCGGGCTGAGTGGTGGAAGGGAGTGACCCTGAACAAGGGCGACATAGTTGTCTTAG
ATTACACCTTAAGACTGGCT

Product: L-2-aminoadipate N-acetyltransferase

Products: NA

Alternate protein names: AAA--lysW ligase lysX [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MKVGIAYDHPRWEEKHIIELLRSRGHEVIEYPLRHSSFEIGVSSELPDVVVQRAVSSARAISFTAHMESMGVPVVNSLHT
QLVCDNKVLTDSALNKENVPRPRTFIAYDLESALEAARELGFPLVVKPLQGSWGRLQALVKDEDALKAIIEHREAMPSPQ
FKVHYLQEYINKPNRDIRVFVVGDSVPVAIYRISEREWRTNTALGGRAEKAEVDEELEELAIKAARAVGGGVLGVDVVED
PERGYLVIEVNSNVDFKNTYKVTGFDMGEAIIDYALSLVKR

Sequences:

>Translated_281_residues
MKVGIAYDHPRWEEKHIIELLRSRGHEVIEYPLRHSSFEIGVSSELPDVVVQRAVSSARAISFTAHMESMGVPVVNSLHT
QLVCDNKVLTDSALNKENVPRPRTFIAYDLESALEAARELGFPLVVKPLQGSWGRLQALVKDEDALKAIIEHREAMPSPQ
FKVHYLQEYINKPNRDIRVFVVGDSVPVAIYRISEREWRTNTALGGRAEKAEVDEELEELAIKAARAVGGGVLGVDVVED
PERGYLVIEVNSNVDFKNTYKVTGFDMGEAIIDYALSLVKR
>Mature_281_residues
MKVGIAYDHPRWEEKHIIELLRSRGHEVIEYPLRHSSFEIGVSSELPDVVVQRAVSSARAISFTAHMESMGVPVVNSLHT
QLVCDNKVLTDSALNKENVPRPRTFIAYDLESALEAARELGFPLVVKPLQGSWGRLQALVKDEDALKAIIEHREAMPSPQ
FKVHYLQEYINKPNRDIRVFVVGDSVPVAIYRISEREWRTNTALGGRAEKAEVDEELEELAIKAARAVGGGVLGVDVVED
PERGYLVIEVNSNVDFKNTYKVTGFDMGEAIIDYALSLVKR

Specific function: Catalyzes the ATP-dependent binding between the amino group of alpha-aminoadipate (AAA) and the gamma-carboxyl group of the C-terminal glutamate residue of lysW (Probable) [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI164663810, Length=247, Percent_Identity=31.5789473684211, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI134254462, Length=243, Percent_Identity=32.0987654320988, Blast_Score=89, Evalue=5e-18,
Organism=Escherichia coli, GI1787076, Length=193, Percent_Identity=33.160621761658, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011870
- InterPro:   IPR016185
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 31474; Mature: 31474

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGIAYDHPRWEEKHIIELLRSRGHEVIEYPLRHSSFEIGVSSELPDVVVQRAVSSARA
CEEECCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHH
ISFTAHMESMGVPVVNSLHTQLVCDNKVLTDSALNKENVPRPRTFIAYDLESALEAAREL
EEEEHHHHHCCCCHHHHHHHHEEECCCEECCCCCCCCCCCCCCEEEEECHHHHHHHHHHC
GFPLVVKPLQGSWGRLQALVKDEDALKAIIEHREAMPSPQFKVHYLQEYINKPNRDIRVF
CCCEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEE
VVGDSVPVAIYRISEREWRTNTALGGRAEKAEVDEELEELAIKAARAVGGGVLGVDVVED
EECCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEC
PERGYLVIEVNSNVDFKNTYKVTGFDMGEAIIDYALSLVKR
CCCCEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVGIAYDHPRWEEKHIIELLRSRGHEVIEYPLRHSSFEIGVSSELPDVVVQRAVSSARA
CEEECCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHH
ISFTAHMESMGVPVVNSLHTQLVCDNKVLTDSALNKENVPRPRTFIAYDLESALEAAREL
EEEEHHHHHCCCCHHHHHHHHEEECCCEECCCCCCCCCCCCCCEEEEECHHHHHHHHHHC
GFPLVVKPLQGSWGRLQALVKDEDALKAIIEHREAMPSPQFKVHYLQEYINKPNRDIRVF
CCCEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEE
VVGDSVPVAIYRISEREWRTNTALGGRAEKAEVDEELEELAIKAARAVGGGVLGVDVVED
EECCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEC
PERGYLVIEVNSNVDFKNTYKVTGFDMGEAIIDYALSLVKR
CCCCEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726; 12042311 [H]