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The map label for this gene is malS [H]

Identifier: 154687127

GI number: 154687127

Start: 2824024

End: 2825721

Strand: Reverse

Name: malS [H]

Synonym: RBAM_026990

Alternate gene names: 154687127

Gene position: 2825721-2824024 (Counterclockwise)

Preceding gene: 154687128

Following gene: 154687125

Centisome position: 72.11

GC content: 51.71

Gene sequence:

>1698_bases
GTGAATCAATTTCAAGTGACGAAAGATGGAGAAATCAAAACAACACTGCGCGGATTAGAAGTGCTTTCTACGCCTTTTTT
AAATAAAGGGGTGGCCTTTACACAGGAGGAAAGAAAGGAACTCGGGCTCGAGGGGTTTCTGCCGCCGAAAGTATTGACAA
TCGAAGAGCAGGCGAGGCGGGCCTACGAACAATTCAGTTCGCAGCCTGATGAGCTGAGCAAAAACGTGTACTTGACCGCT
CTCCATGACCGGAATGAAACGTTATTTTACAGGCTGTTAAATGATCATTTAGGCGAAATGCTGCCGATCGTATATACGCC
GACCGTCGGCACCGCGATCCAGCGTTACAGCCATGAGTACAGAAAACCCCGCGGTCTGTATTTGTCAATAGATGATCCTG
AGGGAATGCGCGCCGCATTTCAGCAGTTCGGAAAAAATGAAAACATTGACTTAATCGTTGCCACAGATGCGGAGGGCATT
CTGGGAATCGGTGACTGGGGCGTCGGCGGCATCGCCATTTCTGTCGGAAAACTCGCGGTGTATACGGCGGCGGCCGGAAT
TGATCCATCACGAGTGCTTGCGGTCGTATTGGACGCGGGGACAAATCAGGAAAGCCTCTTAAATGACCCGCTGTATGTCG
GAAACCAGCATTCAAGAGTGCGCGGCGAGAAATATGACCGCTTTATTGATGAGTATGTCTCGCTTGCCCGGGAAACATTC
CCGAATGCGCTGCTTCATTGGGAGGATTTCGGTACGGCTAACGCCCGCGGCATATTAAAACGGTATAAAGATAAAGTCTG
TACGTTTAACGATGACATTCAAGGAACGGGCGCGGTATCGCTTGCAGCTGTTTTAGCGTGTGCAAAAGCATCAAAAGTGC
CTTTAAAAGAGCATAAAGTCGTCGTCTTCGGCGCGGGAACGGCCGGTATCGGAATTGCTGAGCAGCTGCGCGAAGCCATG
GTGCATGACGGATTAAGCGAAGAAGAATCCTACAGCCATTTTTGGTGTATCGACAGAAACGGATTGTTAACGGATGATAT
GAAGGACTTGCGTGATTTTCAGGTTCCGTACGCGCGCCCTGCTGATGAAGTGAAAGAGTACGGCCGCAGCGGTGAAGGCG
GATCAATCGATCTGGCGGAGACGGTGCGTCAGGCCAAGCCGACGATTCTGATCGGCACATCCACCGTATCAGGCGCATTT
ACGGAAGAAATCGTCAAAGAAATGGCCTCACATGTCAAACGGCCGGCTATTTTGCCGATGTCAAACCCGACGACATTATC
AGAAGCAAAACCTGAAGATTTGATTAAATGGACGGAAGGCCGCGCGCTTGTCACTACGGGTAGTCCGTTTCCGCCTGTTG
CGTATAAAGGCGTTACCTACCATATCGGACAGGCGAACAACGCGCTCGTATTCCCGGGACTCGGGCTCGGAACGATTGTG
ACAAAAGCAAAGCTCATTACGGACAATATGTTCGTTGCCTGCGCGAATGCCATCGCCGGTATGGTGGACGTCGGCAAGCC
GGGTGCGCCGATGCTTCCGAAAGTGGAGGATCTCAGGACGGTATCGGCAACTGTGGCGGTAGCTGTAGCAAAAGCTGCGA
TTCAGGACGGTGTAGCGGAAACGGAACCCGAGGATCTGATCCAAGCGGTGCAGGACGCGATGTGGCACCCCGTATATAAA
CGAATTCGGCCGATGTAA

Upstream 100 bases:

>100_bases
TGTCAAACGGGAGAAAAAATAAGCGGATCGATGATTTGCCTGTATGAATTTCGGGAATAAAAGCAAACCTAAGACAAATT
GCTTTGAAAGGAAGAGACAG

Downstream 100 bases:

>100_bases
CCGAATAAAGCCCAAAACGCTGATGGTTTTGGGCTTTATTTTTTGCTTGTCCGAATTATGAGGCGACAGAATCAATGACA
TCGACAATCTGGCCTGTTTT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NAD-ME 3 [H]

Number of amino acids: Translated: 565; Mature: 565

Protein sequence:

>565_residues
MNQFQVTKDGEIKTTLRGLEVLSTPFLNKGVAFTQEERKELGLEGFLPPKVLTIEEQARRAYEQFSSQPDELSKNVYLTA
LHDRNETLFYRLLNDHLGEMLPIVYTPTVGTAIQRYSHEYRKPRGLYLSIDDPEGMRAAFQQFGKNENIDLIVATDAEGI
LGIGDWGVGGIAISVGKLAVYTAAAGIDPSRVLAVVLDAGTNQESLLNDPLYVGNQHSRVRGEKYDRFIDEYVSLARETF
PNALLHWEDFGTANARGILKRYKDKVCTFNDDIQGTGAVSLAAVLACAKASKVPLKEHKVVVFGAGTAGIGIAEQLREAM
VHDGLSEEESYSHFWCIDRNGLLTDDMKDLRDFQVPYARPADEVKEYGRSGEGGSIDLAETVRQAKPTILIGTSTVSGAF
TEEIVKEMASHVKRPAILPMSNPTTLSEAKPEDLIKWTEGRALVTTGSPFPPVAYKGVTYHIGQANNALVFPGLGLGTIV
TKAKLITDNMFVACANAIAGMVDVGKPGAPMLPKVEDLRTVSATVAVAVAKAAIQDGVAETEPEDLIQAVQDAMWHPVYK
RIRPM

Sequences:

>Translated_565_residues
MNQFQVTKDGEIKTTLRGLEVLSTPFLNKGVAFTQEERKELGLEGFLPPKVLTIEEQARRAYEQFSSQPDELSKNVYLTA
LHDRNETLFYRLLNDHLGEMLPIVYTPTVGTAIQRYSHEYRKPRGLYLSIDDPEGMRAAFQQFGKNENIDLIVATDAEGI
LGIGDWGVGGIAISVGKLAVYTAAAGIDPSRVLAVVLDAGTNQESLLNDPLYVGNQHSRVRGEKYDRFIDEYVSLARETF
PNALLHWEDFGTANARGILKRYKDKVCTFNDDIQGTGAVSLAAVLACAKASKVPLKEHKVVVFGAGTAGIGIAEQLREAM
VHDGLSEEESYSHFWCIDRNGLLTDDMKDLRDFQVPYARPADEVKEYGRSGEGGSIDLAETVRQAKPTILIGTSTVSGAF
TEEIVKEMASHVKRPAILPMSNPTTLSEAKPEDLIKWTEGRALVTTGSPFPPVAYKGVTYHIGQANNALVFPGLGLGTIV
TKAKLITDNMFVACANAIAGMVDVGKPGAPMLPKVEDLRTVSATVAVAVAKAAIQDGVAETEPEDLIQAVQDAMWHPVYK
RIRPM
>Mature_565_residues
MNQFQVTKDGEIKTTLRGLEVLSTPFLNKGVAFTQEERKELGLEGFLPPKVLTIEEQARRAYEQFSSQPDELSKNVYLTA
LHDRNETLFYRLLNDHLGEMLPIVYTPTVGTAIQRYSHEYRKPRGLYLSIDDPEGMRAAFQQFGKNENIDLIVATDAEGI
LGIGDWGVGGIAISVGKLAVYTAAAGIDPSRVLAVVLDAGTNQESLLNDPLYVGNQHSRVRGEKYDRFIDEYVSLARETF
PNALLHWEDFGTANARGILKRYKDKVCTFNDDIQGTGAVSLAAVLACAKASKVPLKEHKVVVFGAGTAGIGIAEQLREAM
VHDGLSEEESYSHFWCIDRNGLLTDDMKDLRDFQVPYARPADEVKEYGRSGEGGSIDLAETVRQAKPTILIGTSTVSGAF
TEEIVKEMASHVKRPAILPMSNPTTLSEAKPEDLIKWTEGRALVTTGSPFPPVAYKGVTYHIGQANNALVFPGLGLGTIV
TKAKLITDNMFVACANAIAGMVDVGKPGAPMLPKVEDLRTVSATVAVAVAKAAIQDGVAETEPEDLIQAVQDAMWHPVYK
RIRPM

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=556, Percent_Identity=39.7482014388489, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI62420882, Length=548, Percent_Identity=40.6934306569343, Blast_Score=404, Evalue=1e-113,
Organism=Homo sapiens, GI62420880, Length=548, Percent_Identity=40.6934306569343, Blast_Score=404, Evalue=1e-113,
Organism=Homo sapiens, GI239049447, Length=548, Percent_Identity=40.6934306569343, Blast_Score=404, Evalue=1e-113,
Organism=Homo sapiens, GI4505145, Length=556, Percent_Identity=38.8489208633094, Blast_Score=388, Evalue=1e-108,
Organism=Homo sapiens, GI270265879, Length=462, Percent_Identity=41.991341991342, Blast_Score=357, Evalue=2e-98,
Organism=Escherichia coli, GI87081919, Length=545, Percent_Identity=45.8715596330275, Blast_Score=488, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI17537199, Length=555, Percent_Identity=41.2612612612613, Blast_Score=406, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6322823, Length=587, Percent_Identity=41.7376490630324, Blast_Score=479, Evalue=1e-136,
Organism=Drosophila melanogaster, GI21356279, Length=571, Percent_Identity=40.2802101576182, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI281362674, Length=571, Percent_Identity=40.2802101576182, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI281362672, Length=571, Percent_Identity=40.2802101576182, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24646388, Length=566, Percent_Identity=37.4558303886926, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24646386, Length=566, Percent_Identity=37.4558303886926, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI281363505, Length=570, Percent_Identity=34.9122807017544, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI78707236, Length=570, Percent_Identity=34.9122807017544, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI78707242, Length=545, Percent_Identity=36.697247706422, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI78707238, Length=545, Percent_Identity=36.697247706422, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI281363503, Length=543, Percent_Identity=35.3591160220994, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI78707232, Length=541, Percent_Identity=36.0443622920518, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI78707240, Length=521, Percent_Identity=37.236084452975, Blast_Score=358, Evalue=8e-99,
Organism=Drosophila melanogaster, GI19922384, Length=553, Percent_Identity=28.75226039783, Blast_Score=258, Evalue=7e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 61682; Mature: 61682

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQFQVTKDGEIKTTLRGLEVLSTPFLNKGVAFTQEERKELGLEGFLPPKVLTIEEQARR
CCCEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCEEEHHHHHHH
AYEQFSSQPDELSKNVYLTALHDRNETLFYRLLNDHLGEMLPIVYTPTVGTAIQRYSHEY
HHHHHCCCHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHH
RKPRGLYLSIDDPEGMRAAFQQFGKNENIDLIVATDAEGILGIGDWGVGGIAISVGKLAV
CCCCCEEEEECCCCHHHHHHHHHCCCCCEEEEEEECCCCEEEECCCCCCCEEEEHHHHEE
YTAAAGIDPSRVLAVVLDAGTNQESLLNDPLYVGNQHSRVRGEKYDRFIDEYVSLARETF
EHHHCCCCHHHEEEEEEECCCCHHHHCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHC
PNALLHWEDFGTANARGILKRYKDKVCTFNDDIQGTGAVSLAAVLACAKASKVPLKEHKV
CCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEE
VVFGAGTAGIGIAEQLREAMVHDGLSEEESYSHFWCIDRNGLLTDDMKDLRDFQVPYARP
EEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCCCCCC
ADEVKEYGRSGEGGSIDLAETVRQAKPTILIGTSTVSGAFTEEIVKEMASHVKRPAILPM
HHHHHHHCCCCCCCCEEHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEC
SNPTTLSEAKPEDLIKWTEGRALVTTGSPFPPVAYKGVTYHIGQANNALVFPGLGLGTIV
CCCCCCCCCCHHHHHEECCCCEEEECCCCCCCCEECCCEEEECCCCCEEEECCCCHHHHH
TKAKLITDNMFVACANAIAGMVDVGKPGAPMLPKVEDLRTVSATVAVAVAKAAIQDGVAE
HHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
TEPEDLIQAVQDAMWHPVYKRIRPM
CCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNQFQVTKDGEIKTTLRGLEVLSTPFLNKGVAFTQEERKELGLEGFLPPKVLTIEEQARR
CCCEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCEEEHHHHHHH
AYEQFSSQPDELSKNVYLTALHDRNETLFYRLLNDHLGEMLPIVYTPTVGTAIQRYSHEY
HHHHHCCCHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHH
RKPRGLYLSIDDPEGMRAAFQQFGKNENIDLIVATDAEGILGIGDWGVGGIAISVGKLAV
CCCCCEEEEECCCCHHHHHHHHHCCCCCEEEEEEECCCCEEEECCCCCCCEEEEHHHHEE
YTAAAGIDPSRVLAVVLDAGTNQESLLNDPLYVGNQHSRVRGEKYDRFIDEYVSLARETF
EHHHCCCCHHHEEEEEEECCCCHHHHCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHC
PNALLHWEDFGTANARGILKRYKDKVCTFNDDIQGTGAVSLAAVLACAKASKVPLKEHKV
CCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEE
VVFGAGTAGIGIAEQLREAMVHDGLSEEESYSHFWCIDRNGLLTDDMKDLRDFQVPYARP
EEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCCCCCC
ADEVKEYGRSGEGGSIDLAETVRQAKPTILIGTSTVSGAFTEEIVKEMASHVKRPAILPM
HHHHHHHCCCCCCCCEEHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEC
SNPTTLSEAKPEDLIKWTEGRALVTTGSPFPPVAYKGVTYHIGQANNALVFPGLGLGTIV
CCCCCCCCCCHHHHHEECCCCEEEECCCCCCCCEECCCEEEECCCCCEEEECCCCHHHHH
TKAKLITDNMFVACANAIAGMVDVGKPGAPMLPKVEDLRTVSATVAVAVAKAAIQDGVAE
HHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
TEPEDLIQAVQDAMWHPVYKRIRPM
CCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]