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The map label for this gene is tyrS [H]

Identifier: 154687107

GI number: 154687107

Start: 2804175

End: 2805440

Strand: Reverse

Name: tyrS [H]

Synonym: RBAM_026790

Alternate gene names: 154687107

Gene position: 2805440-2804175 (Counterclockwise)

Preceding gene: 154687108

Following gene: 154687103

Centisome position: 71.59

GC content: 46.76

Gene sequence:

>1266_bases
ATGACTAACTTATTAGAGGATTTATCATTCCGCGGCTTAATCCAGCAAATGACGGATGAAGAAGGACTGAATAAACAGCT
GAACGAGGAAAAAATCCGTTTATACTCAGGGTTTGACCCGACGGCTGACAGCCTGCACATCGGACACCTTCTGCCGATAT
TGACACTGCGCCGTTTCCAGCTTGCAGGCCACCATCCGATTGCGCTTGTAGGCGGCGCGACGGGACTGATCGGCGATCCG
AGCGGCAAAAAAGCGGAACGTACGTTAAATACTCAGGATATCGTTGTCGAATGGTCGCAAAAAATTAAAAATCAGCTTTC
ACGATTTTTAGATTTCGAGGCCGGAGAAAACCCCGCTGTCATAGCGAACAACTTTGATTGGATCGGAAAGCTGAGCATTA
TTGAATTTCTCCGTGACGTCGGAAAGAACTTCGGAATTAACTACATGCTTGCGAAAGATACTGTAAGCTCAAGAATTGAA
ACCGGAATTTCTTACACGGAATTCAGCTACATGATCCTGCAATCCTACGATTTTCTGAATCTGTACAGAGAAAAGAACTG
CAAACTCCAAATCGGAGGCAGTGACCAGTGGGGCAACATCACGGCCGGTCTTGAACTGATCAGAAAATCAGAAGAAGAAG
GGGCGAAAGCTTTCGGTCTTACCATTCCGCTCGTCACAAAAGCTGACGGCACGAAATTCGGCAAAACGGAAGGCGGCGCC
ATTTGGCTTGATAAAGAAAAAACATCGCCGTACGAATTCTACCAGTTCTGGATCAATACAGATGACCGCGACGTCGTCAA
ATACTTAAAATACTTTACTTTCTTATCAAAAGAGGAAATTGAAGCTTATGCTGAAAAAACGGAGACGGCTCCTGAGAAAA
GAGAAGCGCAGAAACGTCTCGCTGAAGAGGTGACTGTTCTTGTACACGGCCGCGAAGCGCTTGAGCAGGCCGTTCATATC
TCCCAGGCGCTGTTCAGCGGCAATATTAAAGAGCTTTCCGCTCAAGACGTCAAGGTAGGATTTAAAGATGTGCCTTCGTT
TGAAAAAGACCGCTCTGAAGAACTGTCTTTAGTAGACGTGCTTGTGGAATCTAAATTGTCGCCTTCAAAACGGCAGGCCC
GCGAAGACATTCAAAACGGAGCTGTTTACATTAACGGCGAGCGCCAGACGGACATCGGGCATCTTCTGACTGCGGAAGAC
CGCATTGAAGATCAATTTACCGTACTGCGCCGCGGAAAGAAAAAATACTTCCTGATCACGTACTGA

Upstream 100 bases:

>100_bases
TTTTTATGCTTTTATTAGTAGGGTGGTACCGCGATTATATCGTCCCTTCGTTTTAAACGAAGGGGCGTTTTTTATTTTCA
TAAACAAAGGAGCAGACAAA

Downstream 100 bases:

>100_bases
AAATAAAAAGCAGTCCGGCCGCTCAATGCGGCAGGCTGCTTTTTTATGTTTTGTGAATACTGCACGGGGAAATGAGTACA
GTACATAGTATAAGCAAAAC

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase 1; TyrRS 1 [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MTNLLEDLSFRGLIQQMTDEEGLNKQLNEEKIRLYSGFDPTADSLHIGHLLPILTLRRFQLAGHHPIALVGGATGLIGDP
SGKKAERTLNTQDIVVEWSQKIKNQLSRFLDFEAGENPAVIANNFDWIGKLSIIEFLRDVGKNFGINYMLAKDTVSSRIE
TGISYTEFSYMILQSYDFLNLYREKNCKLQIGGSDQWGNITAGLELIRKSEEEGAKAFGLTIPLVTKADGTKFGKTEGGA
IWLDKEKTSPYEFYQFWINTDDRDVVKYLKYFTFLSKEEIEAYAEKTETAPEKREAQKRLAEEVTVLVHGREALEQAVHI
SQALFSGNIKELSAQDVKVGFKDVPSFEKDRSEELSLVDVLVESKLSPSKRQAREDIQNGAVYINGERQTDIGHLLTAED
RIEDQFTVLRRGKKKYFLITY

Sequences:

>Translated_421_residues
MTNLLEDLSFRGLIQQMTDEEGLNKQLNEEKIRLYSGFDPTADSLHIGHLLPILTLRRFQLAGHHPIALVGGATGLIGDP
SGKKAERTLNTQDIVVEWSQKIKNQLSRFLDFEAGENPAVIANNFDWIGKLSIIEFLRDVGKNFGINYMLAKDTVSSRIE
TGISYTEFSYMILQSYDFLNLYREKNCKLQIGGSDQWGNITAGLELIRKSEEEGAKAFGLTIPLVTKADGTKFGKTEGGA
IWLDKEKTSPYEFYQFWINTDDRDVVKYLKYFTFLSKEEIEAYAEKTETAPEKREAQKRLAEEVTVLVHGREALEQAVHI
SQALFSGNIKELSAQDVKVGFKDVPSFEKDRSEELSLVDVLVESKLSPSKRQAREDIQNGAVYINGERQTDIGHLLTAED
RIEDQFTVLRRGKKKYFLITY
>Mature_420_residues
TNLLEDLSFRGLIQQMTDEEGLNKQLNEEKIRLYSGFDPTADSLHIGHLLPILTLRRFQLAGHHPIALVGGATGLIGDPS
GKKAERTLNTQDIVVEWSQKIKNQLSRFLDFEAGENPAVIANNFDWIGKLSIIEFLRDVGKNFGINYMLAKDTVSSRIET
GISYTEFSYMILQSYDFLNLYREKNCKLQIGGSDQWGNITAGLELIRKSEEEGAKAFGLTIPLVTKADGTKFGKTEGGAI
WLDKEKTSPYEFYQFWINTDDRDVVKYLKYFTFLSKEEIEAYAEKTETAPEKREAQKRLAEEVTVLVHGREALEQAVHIS
QALFSGNIKELSAQDVKVGFKDVPSFEKDRSEELSLVDVLVESKLSPSKRQAREDIQNGAVYINGERQTDIGHLLTAEDR
IEDQFTVLRRGKKKYFLITY

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=440, Percent_Identity=37.7272727272727, Blast_Score=261, Evalue=7e-70,
Organism=Escherichia coli, GI1787925, Length=424, Percent_Identity=54.4811320754717, Blast_Score=475, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI25145802, Length=434, Percent_Identity=35.9447004608295, Blast_Score=246, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI25145805, Length=337, Percent_Identity=33.5311572700297, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6325160, Length=439, Percent_Identity=37.8132118451025, Blast_Score=288, Evalue=1e-78,
Organism=Drosophila melanogaster, GI20130383, Length=407, Percent_Identity=41.5233415233415, Blast_Score=323, Evalue=1e-88,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 47757; Mature: 47625

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLLEDLSFRGLIQQMTDEEGLNKQLNEEKIRLYSGFDPTADSLHIGHLLPILTLRRFQ
CCCHHHHHHHHHHHHHHHCHHCCHHHCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHH
LAGHHPIALVGGATGLIGDPSGKKAERTLNTQDIVVEWSQKIKNQLSRFLDFEAGENPAV
HCCCCCEEEECCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
IANNFDWIGKLSIIEFLRDVGKNFGINYMLAKDTVSSRIETGISYTEFSYMILQSYDFLN
EECCCCHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LYREKNCKLQIGGSDQWGNITAGLELIRKSEEEGAKAFGLTIPLVTKADGTKFGKTEGGA
HHHCCCCEEEECCCCCCCCHHHHHHHHHHCHHCCCEEEEEEEEEEECCCCCCCCCCCCCE
IWLDKEKTSPYEFYQFWINTDDRDVVKYLKYFTFLSKEEIEAYAEKTETAPEKREAQKRL
EEEECCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
AEEVTVLVHGREALEQAVHISQALFSGNIKELSAQDVKVGFKDVPSFEKDRSEELSLVDV
HHHHEEEEECHHHHHHHHHHHHHHHCCCHHHCCCHHHHCCHHCCCCCCCCCHHHHHHHHH
LVESKLSPSKRQAREDIQNGAVYINGERQTDIGHLLTAEDRIEDQFTVLRRGKKKYFLIT
HHHCCCCCHHHHHHHHHHCCEEEECCCCCCCHHHEECCHHHHHHHHHHHHCCCCCEEEEE
Y
C
>Mature Secondary Structure 
TNLLEDLSFRGLIQQMTDEEGLNKQLNEEKIRLYSGFDPTADSLHIGHLLPILTLRRFQ
CCHHHHHHHHHHHHHHHCHHCCHHHCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHH
LAGHHPIALVGGATGLIGDPSGKKAERTLNTQDIVVEWSQKIKNQLSRFLDFEAGENPAV
HCCCCCEEEECCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
IANNFDWIGKLSIIEFLRDVGKNFGINYMLAKDTVSSRIETGISYTEFSYMILQSYDFLN
EECCCCHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LYREKNCKLQIGGSDQWGNITAGLELIRKSEEEGAKAFGLTIPLVTKADGTKFGKTEGGA
HHHCCCCEEEECCCCCCCCHHHHHHHHHHCHHCCCEEEEEEEEEEECCCCCCCCCCCCCE
IWLDKEKTSPYEFYQFWINTDDRDVVKYLKYFTFLSKEEIEAYAEKTETAPEKREAQKRL
EEEECCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
AEEVTVLVHGREALEQAVHISQALFSGNIKELSAQDVKVGFKDVPSFEKDRSEELSLVDV
HHHHEEEEECHHHHHHHHHHHHHHHCCCHHHCCCHHHHCCHHCCCCCCCCCHHHHHHHHH
LVESKLSPSKRQAREDIQNGAVYINGERQTDIGHLLTAEDRIEDQFTVLRRGKKKYFLIT
HHHCCCCCHHHHHHHHHHCCEEEECCCCCCCHHHEECCHHHHHHHHHHHHCCCCCEEEEE
Y
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1735721; 9387221; 9384377; 1699930 [H]