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The map label for this gene is hmp [H]

Identifier: 154687099

GI number: 154687099

Start: 2796779

End: 2797969

Strand: Reverse

Name: hmp [H]

Synonym: RBAM_026710

Alternate gene names: 154687099

Gene position: 2797969-2796779 (Counterclockwise)

Preceding gene: 154687101

Following gene: 154687098

Centisome position: 71.4

GC content: 44.92

Gene sequence:

>1191_bases
ATGCTCGATGAAAAAACGATTGAAATCATTAAAAGCACAGTGCCGGTCTTAGAAGAGCATGGTGAAGAAATCACAGGACG
ATTTTATGACCTGCTGTTTCAGCATCACCCTGAGCTGCTGAATATCTTTAATCAGACAAATCAAAAGAAAAAAACACAGC
GTACGGCCTTGTCGAATGCTGTGATTGCAGCCGCAGCCAATATTGACCGGCTGGAAAACATCGTTCCCGTCGTCAAACAA
ATCAGCCATAAACACCGGAGTATCGGTGTTAAGCCCGAGCATTACCCAATCGTTGGAAAGTACTTGTTAATCGCGATAAA
GGATGTGCTAAAAGACGGGGCCGCTCCTGAGGTCATTCAGGCATGGGAAAAAGCATACGGCGTCATTGCAGACGCTTTTA
TCGGAATTGAGAAGGACATGTATGAACAAGCGGAAACGCAAAAAGGCGGCTGGAAAGACTATAAGGCGTTTCAAATTGAT
AAAAAGGTAAAAGAAAGCAAAGAGATAACGTCGTTTTATTTAAAACCGGAAGACGGACAAGTTCTCCCCGAATTCAAAGC
GGGGCAGTATATCAGTGTAAAAGTATACATTCCGGACACGGGCTATACGCATATCCGCCAATACAGCTTATCCGACGTGT
CCGGTAAGGATTATTACCGGATTTCCGTGAAAAAAGAAGGGATTGTTTCCTCATATCTGCATGACCAATTACACGAAAAG
GATTCAATTGAGATCAGCGCTCCGGCCGGGGATTTCGTATTAGACCCTGATTCCAAAAAGAATCTGGTTCTCATCAGTGC
CGGTGTCGGAATTACCCCGATGATCAGCATGTTAAAGACGGTTGCCAAAACCCAGCCGCAGCGAAACGTTCTCTTTATCC
ATGCGGCAAGGAGCGGAGAGTATCATGCGTTGCGGCATGAAGCTGAAGAAGCCGCGGAACACTCATCCGTTGACACCCTC
TTTATTTACAGTGAGCCGTCCGAACAAGACCGTTCAGAAAACATTCGTTTTCGGGAAGGCCGGATTGACAAACAGCTGTT
AGAAGAAGTGATGACAGATAAAGATGCTGATTTTTACCTGTGCGGATCACCGTCGTTCATTAAAGAAATGAAAAAGCTGA
TTTCTGAATTCAATACACCGCCGGAATCCATTCACTATGAATTATTTGAACCGCAGCTGAGTATGGTGTGA

Upstream 100 bases:

>100_bases
GCTAACCCTTTTTGAGCGGGGAAATCTATAAAAAAGAATTCAAAAACGATTCTTTTCTCAGGTCAATCGAGATGCATACA
TCTTTAGGGGGATTTTTAGA

Downstream 100 bases:

>100_bases
TTGAAGAAGGAAGGCCGATGATTTTAATGTATGAAGCACCCGGGTTGGGTGCTTTTTTTATTCTCTTGCAAGGAAGGGCT
GAATTAAGTGCCATATTAGT

Product: nitric oxide dioxygenase

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MLDEKTIEIIKSTVPVLEEHGEEITGRFYDLLFQHHPELLNIFNQTNQKKKTQRTALSNAVIAAAANIDRLENIVPVVKQ
ISHKHRSIGVKPEHYPIVGKYLLIAIKDVLKDGAAPEVIQAWEKAYGVIADAFIGIEKDMYEQAETQKGGWKDYKAFQID
KKVKESKEITSFYLKPEDGQVLPEFKAGQYISVKVYIPDTGYTHIRQYSLSDVSGKDYYRISVKKEGIVSSYLHDQLHEK
DSIEISAPAGDFVLDPDSKKNLVLISAGVGITPMISMLKTVAKTQPQRNVLFIHAARSGEYHALRHEAEEAAEHSSVDTL
FIYSEPSEQDRSENIRFREGRIDKQLLEEVMTDKDADFYLCGSPSFIKEMKKLISEFNTPPESIHYELFEPQLSMV

Sequences:

>Translated_396_residues
MLDEKTIEIIKSTVPVLEEHGEEITGRFYDLLFQHHPELLNIFNQTNQKKKTQRTALSNAVIAAAANIDRLENIVPVVKQ
ISHKHRSIGVKPEHYPIVGKYLLIAIKDVLKDGAAPEVIQAWEKAYGVIADAFIGIEKDMYEQAETQKGGWKDYKAFQID
KKVKESKEITSFYLKPEDGQVLPEFKAGQYISVKVYIPDTGYTHIRQYSLSDVSGKDYYRISVKKEGIVSSYLHDQLHEK
DSIEISAPAGDFVLDPDSKKNLVLISAGVGITPMISMLKTVAKTQPQRNVLFIHAARSGEYHALRHEAEEAAEHSSVDTL
FIYSEPSEQDRSENIRFREGRIDKQLLEEVMTDKDADFYLCGSPSFIKEMKKLISEFNTPPESIHYELFEPQLSMV
>Mature_396_residues
MLDEKTIEIIKSTVPVLEEHGEEITGRFYDLLFQHHPELLNIFNQTNQKKKTQRTALSNAVIAAAANIDRLENIVPVVKQ
ISHKHRSIGVKPEHYPIVGKYLLIAIKDVLKDGAAPEVIQAWEKAYGVIADAFIGIEKDMYEQAETQKGGWKDYKAFQID
KKVKESKEITSFYLKPEDGQVLPEFKAGQYISVKVYIPDTGYTHIRQYSLSDVSGKDYYRISVKKEGIVSSYLHDQLHEK
DSIEISAPAGDFVLDPDSKKNLVLISAGVGITPMISMLKTVAKTQPQRNVLFIHAARSGEYHALRHEAEEAAEHSSVDTL
FIYSEPSEQDRSENIRFREGRIDKQLLEEVMTDKDADFYLCGSPSFIKEMKKLISEFNTPPESIHYELFEPQLSMV

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=396, Percent_Identity=41.1616161616162, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI1788104, Length=243, Percent_Identity=25.1028806584362, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787658, Length=249, Percent_Identity=21.285140562249, Blast_Score=62, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6321673, Length=416, Percent_Identity=41.5865384615385, Blast_Score=269, Evalue=5e-73,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 44993; Mature: 44993

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS01033 GLOBIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDEKTIEIIKSTVPVLEEHGEEITGRFYDLLFQHHPELLNIFNQTNQKKKTQRTALSNA
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHH
VIAAAANIDRLENIVPVVKQISHKHRSIGVKPEHYPIVGKYLLIAIKDVLKDGAAPEVIQ
HHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHH
AWEKAYGVIADAFIGIEKDMYEQAETQKGGWKDYKAFQIDKKVKESKEITSFYLKPEDGQ
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCC
VLPEFKAGQYISVKVYIPDTGYTHIRQYSLSDVSGKDYYRISVKKEGIVSSYLHDQLHEK
CCCCCCCCCEEEEEEEECCCCCHHHHEECCCCCCCCCEEEEEEECCHHHHHHHHHHHCCC
DSIEISAPAGDFVLDPDSKKNLVLISAGVGITPMISMLKTVAKTQPQRNVLFIHAARSGE
CCEEEECCCCCEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
YHALRHEAEEAAEHSSVDTLFIYSEPSEQDRSENIRFREGRIDKQLLEEVMTDKDADFYL
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHCCCEEECCCCCHHHHHHHHCCCCCCEEE
CGSPSFIKEMKKLISEFNTPPESIHYELFEPQLSMV
ECCCHHHHHHHHHHHHHCCCHHHHHHHEECCCCCCC
>Mature Secondary Structure
MLDEKTIEIIKSTVPVLEEHGEEITGRFYDLLFQHHPELLNIFNQTNQKKKTQRTALSNA
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHH
VIAAAANIDRLENIVPVVKQISHKHRSIGVKPEHYPIVGKYLLIAIKDVLKDGAAPEVIQ
HHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHH
AWEKAYGVIADAFIGIEKDMYEQAETQKGGWKDYKAFQIDKKVKESKEITSFYLKPEDGQ
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCC
VLPEFKAGQYISVKVYIPDTGYTHIRQYSLSDVSGKDYYRISVKKEGIVSSYLHDQLHEK
CCCCCCCCCEEEEEEEECCCCCHHHHEECCCCCCCCCEEEEEEECCHHHHHHHHHHHCCC
DSIEISAPAGDFVLDPDSKKNLVLISAGVGITPMISMLKTVAKTQPQRNVLFIHAARSGE
CCEEEECCCCCEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
YHALRHEAEEAAEHSSVDTLFIYSEPSEQDRSENIRFREGRIDKQLLEEVMTDKDADFYL
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHCCCEEECCCCCHHHHHHHHCCCCCCEEE
CGSPSFIKEMKKLISEFNTPPESIHYELFEPQLSMV
ECCCHHHHHHHHHHHHHCCCHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8682784; 9384377 [H]