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The map label for this gene is ytsJ [H]

Identifier: 154687055

GI number: 154687055

Start: 2753831

End: 2755063

Strand: Reverse

Name: ytsJ [H]

Synonym: RBAM_026270

Alternate gene names: 154687055

Gene position: 2755063-2753831 (Counterclockwise)

Preceding gene: 154687056

Following gene: 154687054

Centisome position: 70.31

GC content: 49.47

Gene sequence:

>1233_bases
ATGTCATTAAGAGAAGAAGCGTTACATTTACACAAAGTAAATCAAGGAAAGCTGGAATCAAAAGCAAAGGTAGAAGTCAG
AAATGCAGATGACTTAAGCCTTGCCTACTCTCCCGGAGTAGCTGAGCCTTGTAAAGAGATTTATGACGATAAAAACAAAG
TATATGATTATACAATGAAGGGGAACATGGTAGCAGTCGTAACAGACGGATCAGCCGTTCTCGGTCTCGGTAATATCGGG
CCTGAAGCTTCACTTCCGGTCATGGAAGGGAAAGCGGTGCTGTTCAAAAGTTTCGCGGGCGTGGATGCATTCCCGATCGC
GCTTGACACAAACGATGTGGACAAAATCATTGACACTGTAAAGCTGCTTGCACCGACATTCGGCGGTGTCAACCTTGAAG
ATATTGCGGCGCCGAACTGCTTCATTATCGAAGAGCGCCTGAAAAAAGAAACAAACATTCCGGTTTTCCACGATGATCAG
CACGGCACTGCCATTGTAACCGTTGCCGGACTTGTAAACGCGCTGAAATTATCAGGTAAATCCATGTCATCCATTAAAGT
TGTGGCAAACGGAGCGGGAGCGGCAGGCATCGCGATCATCAAGCTTCTTTACCATTTCGGCGTACGCGACATCGTAATGT
GCGACTCTAAAGGGGCGATTTACGAAGGGCGTCCGGCCGGCATGAACGCAGTGAAAAACGAAGTGGCGAAGTTTACAAAC
CAAGACCGCAACGACGGTTCGCTGCAGGATGTCATTAAAGATGCAGACGTCTTTATCGGCGTATCAGTCGAAGGCGCGCT
GACGAAAGAAATGGTGGAAAGCATGGCGAAAGATCCGATTATCTTCGCAATGGCCAACCCGAATCCGGAAATCATGCCTG
AAGACGCGCGCGCAGCCGGCGCAAGCGTAATCGGAACAGGCCGTTCTGATTTCCCGAACCAGGTCAACAACGTCCTGGCA
TTCCCTGGAATTTTCCGCGGAGCGCTTGACGTTCGCGCGACTCACATCAACGAAGAAATGAAAATTGCTGCGGTTGAAGC
GATTGCTTCCCTTGTTACAGAGGAAGAATTAAGCGCTGATTATGTCATCCCGGCTCCATTTGACAAACGTGTGGCTCCTG
CCGTTGCGAAAGCTGTTGCGAAAGCAGCGATGGAAACGGGCGTCGCCAGAATTACGGTCGATCCTGAGGAAGTGGCTGAA
AAAACAAGACAGCTCACGGTCATCGGCGAATAA

Upstream 100 bases:

>100_bases
GTGCTATCTTATTTAAAGATTCTGAAATTTTTCACCTCCAGCGATTGTACCAGTCTATGCGGCAGAATATGTAACAAAGT
ACTTAAAAGGAGTGTTTAGA

Downstream 100 bases:

>100_bases
TAACACAAAAATGAAATGGCAGCGCGTTGGAAAAAAACAACGCGCTGCTGTTCAATATACGGACTGCTGGCATTTTTTTA
AGGGCAGGTCTTAAAAAAAT

Product: YtsJ

Products: NA

Alternate protein names: NAD-ME 4 [H]

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MSLREEALHLHKVNQGKLESKAKVEVRNADDLSLAYSPGVAEPCKEIYDDKNKVYDYTMKGNMVAVVTDGSAVLGLGNIG
PEASLPVMEGKAVLFKSFAGVDAFPIALDTNDVDKIIDTVKLLAPTFGGVNLEDIAAPNCFIIEERLKKETNIPVFHDDQ
HGTAIVTVAGLVNALKLSGKSMSSIKVVANGAGAAGIAIIKLLYHFGVRDIVMCDSKGAIYEGRPAGMNAVKNEVAKFTN
QDRNDGSLQDVIKDADVFIGVSVEGALTKEMVESMAKDPIIFAMANPNPEIMPEDARAAGASVIGTGRSDFPNQVNNVLA
FPGIFRGALDVRATHINEEMKIAAVEAIASLVTEEELSADYVIPAPFDKRVAPAVAKAVAKAAMETGVARITVDPEEVAE
KTRQLTVIGE

Sequences:

>Translated_410_residues
MSLREEALHLHKVNQGKLESKAKVEVRNADDLSLAYSPGVAEPCKEIYDDKNKVYDYTMKGNMVAVVTDGSAVLGLGNIG
PEASLPVMEGKAVLFKSFAGVDAFPIALDTNDVDKIIDTVKLLAPTFGGVNLEDIAAPNCFIIEERLKKETNIPVFHDDQ
HGTAIVTVAGLVNALKLSGKSMSSIKVVANGAGAAGIAIIKLLYHFGVRDIVMCDSKGAIYEGRPAGMNAVKNEVAKFTN
QDRNDGSLQDVIKDADVFIGVSVEGALTKEMVESMAKDPIIFAMANPNPEIMPEDARAAGASVIGTGRSDFPNQVNNVLA
FPGIFRGALDVRATHINEEMKIAAVEAIASLVTEEELSADYVIPAPFDKRVAPAVAKAVAKAAMETGVARITVDPEEVAE
KTRQLTVIGE
>Mature_409_residues
SLREEALHLHKVNQGKLESKAKVEVRNADDLSLAYSPGVAEPCKEIYDDKNKVYDYTMKGNMVAVVTDGSAVLGLGNIGP
EASLPVMEGKAVLFKSFAGVDAFPIALDTNDVDKIIDTVKLLAPTFGGVNLEDIAAPNCFIIEERLKKETNIPVFHDDQH
GTAIVTVAGLVNALKLSGKSMSSIKVVANGAGAAGIAIIKLLYHFGVRDIVMCDSKGAIYEGRPAGMNAVKNEVAKFTNQ
DRNDGSLQDVIKDADVFIGVSVEGALTKEMVESMAKDPIIFAMANPNPEIMPEDARAAGASVIGTGRSDFPNQVNNVLAF
PGIFRGALDVRATHINEEMKIAAVEAIASLVTEEELSADYVIPAPFDKRVAPAVAKAVAKAAMETGVARITVDPEEVAEK
TRQLTVIGE

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=404, Percent_Identity=28.7128712871287, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI62420880, Length=404, Percent_Identity=28.7128712871287, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI239049447, Length=404, Percent_Identity=28.7128712871287, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI4505143, Length=395, Percent_Identity=26.0759493670886, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI4505145, Length=412, Percent_Identity=27.4271844660194, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI270265879, Length=324, Percent_Identity=29.0123456790123, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1788806, Length=406, Percent_Identity=52.2167487684729, Blast_Score=400, Evalue=1e-113,
Organism=Escherichia coli, GI87081919, Length=441, Percent_Identity=28.7981859410431, Blast_Score=109, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17537199, Length=453, Percent_Identity=24.9448123620309, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6322823, Length=387, Percent_Identity=28.4237726098191, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21356279, Length=447, Percent_Identity=27.0693512304251, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI281362672, Length=447, Percent_Identity=27.0693512304251, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI281362674, Length=447, Percent_Identity=27.0693512304251, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24646388, Length=447, Percent_Identity=27.7404921700224, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24646386, Length=447, Percent_Identity=27.7404921700224, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI78707242, Length=412, Percent_Identity=26.6990291262136, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI78707238, Length=412, Percent_Identity=26.6990291262136, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI78707240, Length=399, Percent_Identity=26.3157894736842, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281363505, Length=412, Percent_Identity=24.0291262135922, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI78707236, Length=412, Percent_Identity=24.0291262135922, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI281363503, Length=412, Percent_Identity=23.7864077669903, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI78707232, Length=371, Percent_Identity=24.7978436657682, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 43591; Mature: 43460

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLREEALHLHKVNQGKLESKAKVEVRNADDLSLAYSPGVAEPCKEIYDDKNKVYDYTMK
CCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEECCCCHHHHHHHHCCCCCEEEEEEC
GNMVAVVTDGSAVLGLGNIGPEASLPVMEGKAVLFKSFAGVDAFPIALDTNDVDKIIDTV
CCEEEEEECCCEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEECCCHHHHHHHHH
KLLAPTFGGVNLEDIAAPNCFIIEERLKKETNIPVFHDDQHGTAIVTVAGLVNALKLSGK
HHHHCCCCCCCHHHHCCCCEEEEHHHHHHHCCCCEEECCCCCEEEEEHHHHHHHHHCCCC
SMSSIKVVANGAGAAGIAIIKLLYHFGVRDIVMCDSKGAIYEGRPAGMNAVKNEVAKFTN
CCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCEECCCCCCHHHHHHHHHHHHC
QDRNDGSLQDVIKDADVFIGVSVEGALTKEMVESMAKDPIIFAMANPNPEIMPEDARAAG
CCCCCCHHHHHHCCCCEEEEEEECHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHCC
ASVIGTGRSDFPNQVNNVLAFPGIFRGALDVRATHINEEMKIAAVEAIASLVTEEELSAD
CEEEECCCCCCCHHHHHEEECCHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHCCCC
YVIPAPFDKRVAPAVAKAVAKAAMETGVARITVDPEEVAEKTRQLTVIGE
EEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHCEEEECC
>Mature Secondary Structure 
SLREEALHLHKVNQGKLESKAKVEVRNADDLSLAYSPGVAEPCKEIYDDKNKVYDYTMK
CHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEECCCCHHHHHHHHCCCCCEEEEEEC
GNMVAVVTDGSAVLGLGNIGPEASLPVMEGKAVLFKSFAGVDAFPIALDTNDVDKIIDTV
CCEEEEEECCCEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEECCCHHHHHHHHH
KLLAPTFGGVNLEDIAAPNCFIIEERLKKETNIPVFHDDQHGTAIVTVAGLVNALKLSGK
HHHHCCCCCCCHHHHCCCCEEEEHHHHHHHCCCCEEECCCCCEEEEEHHHHHHHHHCCCC
SMSSIKVVANGAGAAGIAIIKLLYHFGVRDIVMCDSKGAIYEGRPAGMNAVKNEVAKFTN
CCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCEECCCCCCHHHHHHHHHHHHC
QDRNDGSLQDVIKDADVFIGVSVEGALTKEMVESMAKDPIIFAMANPNPEIMPEDARAAG
CCCCCCHHHHHHCCCCEEEEEEECHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHCC
ASVIGTGRSDFPNQVNNVLAFPGIFRGALDVRATHINEEMKIAAVEAIASLVTEEELSAD
CEEEECCCCCCCHHHHHEEECCHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHCCCC
YVIPAPFDKRVAPAVAKAVAKAAMETGVARITVDPEEVAEKTRQLTVIGE
EEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]