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The map label for this gene is pfkA

Identifier: 154687052

GI number: 154687052

Start: 2750517

End: 2751476

Strand: Reverse

Name: pfkA

Synonym: RBAM_026240

Alternate gene names: 154687052

Gene position: 2751476-2750517 (Counterclockwise)

Preceding gene: 154687053

Following gene: 154687051

Centisome position: 70.22

GC content: 47.81

Gene sequence:

>960_bases
ATGAAGCGTATAGGAGTTTTAACGAGCGGCGGGGATTCCCCGGGTATGAACGCTGCGGTGCGCGCGGTTGTGCGGAAAGC
TATCTACCACGACGTCGAAGTGTACGGAATTTATAACGGTTATTCAGGATTAATCAGCGGAAAAATAGAGAAACTGGAGC
TTGGTTCGGTCGGTGACATCATCCACCGCGGCGGAACAAAGCTGTATACGGCCAGATGTCCTGAATTCAAAACAGTTGAA
GGCCGTGAAAAAGGAATCGAAAATTTAAAGAAACTCGGCATTGAAGGTCTTGTCGTCATCGGCGGAGACGGTTCATACAT
GGGTGCGAAAAAATTAACGGAACACGGGTTTCCGTGTGTAGGTGTACCGGGTACGATAGATAATGACATCCCGGGAACTG
ATTTTACGATCGGATTTGATACGGCGTTAAACACCGTAATTGATGCGATTGATAAAATCCGTGACACCGCTACGTCTCAT
GAGCGTACATACGTTATTGAAGTGATGGGGCGCCATGCGGGCGACATTGCTTTATGGGCAGGTCTTGCAGGCGGAGCGGA
ATCAATTTTAATTCCTGAAGCGGACTACGATATGCAGGAAATTATCGGCCGTTTGAAGAGAGGGCACGACCGCGGCAAAA
AACACAGTATCATCATCGTTGCAGAAGGGGTAGGCAGCGGGGTTGAATTCGGAAAACGCATCGAAGAAGAAACGAACCTT
GAAACACGGGTATCCGTTTTAGGGCACATCCAGCGCGGCGGATCTCCAAGCGCTTCTGACCGGGTTCTTGCAAGCCGCCT
CGGCGCTTACGCGGTTGAGCTTCTGCTTAAAGGAAAAGGCGGACGCTGCGTAGGTATACAAAACAACAAGCTTGTAGACC
ATGATATTATAGAAATTTTAGAATCAAAACATACTGTTGAACCAAACATGTATCAGCTTTCAAAAGAACTGTCTATTTAA

Upstream 100 bases:

>100_bases
ATAGGTAAGGTTTCATGGGAGCTCAGGCGTCCCTTTTCATTGTTTTAGGGCAATGATCATGATATGTTTAACATATATAT
GTTGCTGAGGTGAATAGGAA

Downstream 100 bases:

>100_bases
GGCGGAAGAAGCTGAAGATTTCAGAAGGAAGTGAACGAGTTGAAAAAAACAAAAATCGTTTGTACAATCGGTCCGGCCAG
TGAAAGTGTAGAAATGCTGA

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKRIGVLTSGGDSPGMNAAVRAVVRKAIYHDVEVYGIYNGYSGLISGKIEKLELGSVGDIIHRGGTKLYTARCPEFKTVE
GREKGIENLKKLGIEGLVVIGGDGSYMGAKKLTEHGFPCVGVPGTIDNDIPGTDFTIGFDTALNTVIDAIDKIRDTATSH
ERTYVIEVMGRHAGDIALWAGLAGGAESILIPEADYDMQEIIGRLKRGHDRGKKHSIIIVAEGVGSGVEFGKRIEEETNL
ETRVSVLGHIQRGGSPSASDRVLASRLGAYAVELLLKGKGGRCVGIQNNKLVDHDIIEILESKHTVEPNMYQLSKELSI

Sequences:

>Translated_319_residues
MKRIGVLTSGGDSPGMNAAVRAVVRKAIYHDVEVYGIYNGYSGLISGKIEKLELGSVGDIIHRGGTKLYTARCPEFKTVE
GREKGIENLKKLGIEGLVVIGGDGSYMGAKKLTEHGFPCVGVPGTIDNDIPGTDFTIGFDTALNTVIDAIDKIRDTATSH
ERTYVIEVMGRHAGDIALWAGLAGGAESILIPEADYDMQEIIGRLKRGHDRGKKHSIIIVAEGVGSGVEFGKRIEEETNL
ETRVSVLGHIQRGGSPSASDRVLASRLGAYAVELLLKGKGGRCVGIQNNKLVDHDIIEILESKHTVEPNMYQLSKELSI
>Mature_319_residues
MKRIGVLTSGGDSPGMNAAVRAVVRKAIYHDVEVYGIYNGYSGLISGKIEKLELGSVGDIIHRGGTKLYTARCPEFKTVE
GREKGIENLKKLGIEGLVVIGGDGSYMGAKKLTEHGFPCVGVPGTIDNDIPGTDFTIGFDTALNTVIDAIDKIRDTATSH
ERTYVIEVMGRHAGDIALWAGLAGGAESILIPEADYDMQEIIGRLKRGHDRGKKHSIIIVAEGVGSGVEFGKRIEEETNL
ETRVSVLGHIQRGGSPSASDRVLASRLGAYAVELLLKGKGGRCVGIQNNKLVDHDIIEILESKHTVEPNMYQLSKELSI

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family

Homologues:

Organism=Homo sapiens, GI11321601, Length=347, Percent_Identity=42.0749279538905, Blast_Score=231, Evalue=8e-61,
Organism=Homo sapiens, GI48762920, Length=347, Percent_Identity=41.7867435158501, Blast_Score=224, Evalue=6e-59,
Organism=Homo sapiens, GI266453768, Length=349, Percent_Identity=42.4068767908309, Blast_Score=222, Evalue=3e-58,
Organism=Homo sapiens, GI266453748, Length=349, Percent_Identity=42.4068767908309, Blast_Score=222, Evalue=3e-58,
Organism=Homo sapiens, GI4505749, Length=349, Percent_Identity=42.4068767908309, Blast_Score=222, Evalue=3e-58,
Organism=Homo sapiens, GI266453619, Length=349, Percent_Identity=42.6934097421203, Blast_Score=222, Evalue=3e-58,
Organism=Escherichia coli, GI1790350, Length=318, Percent_Identity=56.6037735849057, Blast_Score=363, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI25147584, Length=347, Percent_Identity=40.6340057636888, Blast_Score=221, Evalue=5e-58,
Organism=Caenorhabditis elegans, GI17558788, Length=309, Percent_Identity=41.1003236245955, Blast_Score=213, Evalue=9e-56,
Organism=Caenorhabditis elegans, GI72003601, Length=319, Percent_Identity=31.974921630094, Blast_Score=160, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6321679, Length=344, Percent_Identity=39.8255813953488, Blast_Score=206, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6323861, Length=338, Percent_Identity=36.9822485207101, Blast_Score=195, Evalue=8e-51,
Organism=Drosophila melanogaster, GI24652337, Length=348, Percent_Identity=41.3793103448276, Blast_Score=232, Evalue=2e-61,
Organism=Drosophila melanogaster, GI17647809, Length=348, Percent_Identity=41.3793103448276, Blast_Score=231, Evalue=4e-61,
Organism=Drosophila melanogaster, GI28573326, Length=347, Percent_Identity=40.9221902017291, Blast_Score=231, Evalue=4e-61,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): K6PF_BACA2 (A7Z7K6)

Other databases:

- EMBL:   CP000560
- RefSeq:   YP_001422213.1
- ProteinModelPortal:   A7Z7K6
- SMR:   A7Z7K6
- STRING:   A7Z7K6
- EnsemblBacteria:   EBBACT00000006060
- GeneID:   5460684
- GenomeReviews:   CP000560_GR
- KEGG:   bay:RBAM_026240
- eggNOG:   COG0205
- GeneTree:   EBGT00050000001727
- HOGENOM:   HBG405044
- OMA:   EADYDMN
- ProtClustDB:   PRK03202
- BioCyc:   BAMY326423:RBAM_026240-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00339
- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023
- PIRSF:   PIRSF000532
- PRINTS:   PR00476
- TIGRFAMs:   TIGR02482

Pfam domain/function: PF00365 PFK; SSF53784 Ppfruckinase

EC number: =2.7.1.11

Molecular weight: Translated: 34262; Mature: 34262

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: ACT_SITE 127-127 BINDING 162-162 BINDING 243-243 BINDING 249-249 BINDING 252-252

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIGVLTSGGDSPGMNAAVRAVVRKAIYHDVEVYGIYNGYSGLISGKIEKLELGSVGDI
CCCEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCEEEEECCCHHHH
IHRGGTKLYTARCPEFKTVEGREKGIENLKKLGIEGLVVIGGDGSYMGAKKLTEHGFPCV
HHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHCCCCEE
GVPGTIDNDIPGTDFTIGFDTALNTVIDAIDKIRDTATSHERTYVIEVMGRHAGDIALWA
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEE
GLAGGAESILIPEADYDMQEIIGRLKRGHDRGKKHSIIIVAEGVGSGVEFGKRIEEETNL
CCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCH
ETRVSVLGHIQRGGSPSASDRVLASRLGAYAVELLLKGKGGRCVGIQNNKLVDHDIIEIL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEECCCCEEEECCCCCCCHHHHHHHH
ESKHTVEPNMYQLSKELSI
HHCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MKRIGVLTSGGDSPGMNAAVRAVVRKAIYHDVEVYGIYNGYSGLISGKIEKLELGSVGDI
CCCEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCEEEEECCCHHHH
IHRGGTKLYTARCPEFKTVEGREKGIENLKKLGIEGLVVIGGDGSYMGAKKLTEHGFPCV
HHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHCCCCEE
GVPGTIDNDIPGTDFTIGFDTALNTVIDAIDKIRDTATSHERTYVIEVMGRHAGDIALWA
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEE
GLAGGAESILIPEADYDMQEIIGRLKRGHDRGKKHSIIIVAEGVGSGVEFGKRIEEETNL
CCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCH
ETRVSVLGHIQRGGSPSASDRVLASRLGAYAVELLLKGKGGRCVGIQNNKLVDHDIIEIL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEECCCCEEEECCCCCCCHHHHHHHH
ESKHTVEPNMYQLSKELSI
HHCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA