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The map label for this gene is pyk [H]

Identifier: 154687051

GI number: 154687051

Start: 2748720

End: 2750477

Strand: Reverse

Name: pyk [H]

Synonym: RBAM_026230

Alternate gene names: 154687051

Gene position: 2750477-2748720 (Counterclockwise)

Preceding gene: 154687052

Following gene: 154687050

Centisome position: 70.19

GC content: 49.6

Gene sequence:

>1758_bases
TTGAAAAAAACAAAAATCGTTTGTACAATCGGTCCGGCCAGTGAAAGTGTAGAAATGCTGACAAAATTGATGGAAGCGGG
TATGAACGTTGCTCGTCTGAATTTTTCACACGGTGATTTCGAAGAGCACGGCGCAAGAATCAAAAACATCAGAGAAGCAA
GCAAAAAGCTCGGCAAAAACGTGGGGATTCTCCTCGATACGAAAGGTCCGGAAATCCGCACGCACGATATGGAAAACGGC
GCGCTTGAGCTTCAGGCGGGAAATGAAATTATCGTTTCTACGAAACAGGTGCTGGGAACACTTGGAAAATTCTCAGTTTC
TTATGAAGGCCTGGCAGACGACGTGTCAGCAGGGTCTATCATCCTGTTGGATGACGGTTTAATCGGATTGGAAGTGCTAG
AATCAAATCCGGAAAAACACGAAATCAAAACAAAAATCTTAAACAGCGGCACGCTGAAAAATAAAAAAGGCGTAAACGTT
CCCGGCGTCAGCGTCAACCTTCCCGGAATTACTGAAAAAGACGCCAAAGACATCGTATTCGGTATCGAGCAAGGCGTTGA
TTTCATCGCTGCGTCATTTGTACGCCGTTCTACGGATGTCCTGGAAATCCGCGAACTGCTTGAAGAGCATAACGCGTCTG
ACATTCAAATCATTCCTAAGATCGAGAACCAGGAAGGCGTGGACAACCTGGATGCGATCCTGGAAGTATCTGACGGCTTA
ATGGTAGCGCGCGGAGACCTCGGCGTAGAGATTCCGGCCGAAGAAGTGCCGCTCGTGCAAAAAGAAATGATCAAAAAATG
CAACGCGCTCGGCAAACCGGTTATTACTGCGACACAAATGCTTGACAGCATGCAGCGCAACCCTCGTCCGACACGCGCTG
AAGCAAGTGACGTTGCCAACGCCATTTTCGACGGCACAGATGCTATCATGCTTTCCGGTGAGACGGCTGCGGGACAATAT
CCTGTCGAGGCGGTTCAAACAATGTTTAACATCGCCACACGCACGGAAGAATCTCTGAACTACAAAGAAATTCTGTCTAA
ACGCAGAGATCAGGTCGGCATGACGATTACCGATGCGATCGGCCAATCAGTTGCGCACACGGCGATCAATCTGAGTGCGG
CAGCTATCGTTACGCCTACAGAAAGCGGCCATACGGCGCGTATGATCGCGAAATACCGTCCGCAGGCTCCGATTGTAGCC
GTTACGGTTAATGAGTCTGTGTCACGCAAACTCGGCCTTGTATTCGGTGTATTCCCGTCAAGCGGCCAAAACGCGAATTC
AACCGATGAAATGCTTGAAGACGCGGTTCAAAAATCACTTGACAGCGGCATCGTGAAACGCGGTGATCTGATTGTCATTA
CAGCAGGTTCTGTCGGTGAATCAGGCACGACAAATCTGATGAAAGTCCACACTGTCGGCAGCATTATCGCGAAAGGGCAA
GGCATCGGGCGCAAATCTGCATTCGGTCCGGTCGTTGTTGCAAAAAATGCGAAAGAAGCTGAACAAAAAATGACAGACGG
CGCGGTATTGGTTGCCTCAAGCACTGACCGCGACATGATCGCTTCACTTGAAAAAGCATCAGCGCTCATCACTGAAGAAG
GCGGTCTGACAAGCCATGCAGCCGTTGTCGGTTTAAGCTTGGGCATTCCGGTTATCGTCGGTCTGGAGAACGCAACTTCA
GTTTTAACAGAAGGACAGGTTATCACAGTTGACGCTGCCCGCGGCGCTGTGTATGAAGGCCGCGCAAGCGTTCTTTAA

Upstream 100 bases:

>100_bases
AGAATCAAAACATACTGTTGAACCAAACATGTATCAGCTTTCAAAAGAACTGTCTATTTAAGGCGGAAGAAGCTGAAGAT
TTCAGAAGGAAGTGAACGAG

Downstream 100 bases:

>100_bases
ACAGCTTTAAAAAAGAGAGGGGGGTTCTCCCTTCTCTTTTGTCTTTAGGCTGATTTTCCTATTCCCATGCGGGCGGTGAC
GCGCTTTTAGAAGGGCAGGA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK; Vegetative protein 17; VEG17 [H]

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MKKTKIVCTIGPASESVEMLTKLMEAGMNVARLNFSHGDFEEHGARIKNIREASKKLGKNVGILLDTKGPEIRTHDMENG
ALELQAGNEIIVSTKQVLGTLGKFSVSYEGLADDVSAGSIILLDDGLIGLEVLESNPEKHEIKTKILNSGTLKNKKGVNV
PGVSVNLPGITEKDAKDIVFGIEQGVDFIAASFVRRSTDVLEIRELLEEHNASDIQIIPKIENQEGVDNLDAILEVSDGL
MVARGDLGVEIPAEEVPLVQKEMIKKCNALGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGQY
PVEAVQTMFNIATRTEESLNYKEILSKRRDQVGMTITDAIGQSVAHTAINLSAAAIVTPTESGHTARMIAKYRPQAPIVA
VTVNESVSRKLGLVFGVFPSSGQNANSTDEMLEDAVQKSLDSGIVKRGDLIVITAGSVGESGTTNLMKVHTVGSIIAKGQ
GIGRKSAFGPVVVAKNAKEAEQKMTDGAVLVASSTDRDMIASLEKASALITEEGGLTSHAAVVGLSLGIPVIVGLENATS
VLTEGQVITVDAARGAVYEGRASVL

Sequences:

>Translated_585_residues
MKKTKIVCTIGPASESVEMLTKLMEAGMNVARLNFSHGDFEEHGARIKNIREASKKLGKNVGILLDTKGPEIRTHDMENG
ALELQAGNEIIVSTKQVLGTLGKFSVSYEGLADDVSAGSIILLDDGLIGLEVLESNPEKHEIKTKILNSGTLKNKKGVNV
PGVSVNLPGITEKDAKDIVFGIEQGVDFIAASFVRRSTDVLEIRELLEEHNASDIQIIPKIENQEGVDNLDAILEVSDGL
MVARGDLGVEIPAEEVPLVQKEMIKKCNALGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGQY
PVEAVQTMFNIATRTEESLNYKEILSKRRDQVGMTITDAIGQSVAHTAINLSAAAIVTPTESGHTARMIAKYRPQAPIVA
VTVNESVSRKLGLVFGVFPSSGQNANSTDEMLEDAVQKSLDSGIVKRGDLIVITAGSVGESGTTNLMKVHTVGSIIAKGQ
GIGRKSAFGPVVVAKNAKEAEQKMTDGAVLVASSTDRDMIASLEKASALITEEGGLTSHAAVVGLSLGIPVIVGLENATS
VLTEGQVITVDAARGAVYEGRASVL
>Mature_585_residues
MKKTKIVCTIGPASESVEMLTKLMEAGMNVARLNFSHGDFEEHGARIKNIREASKKLGKNVGILLDTKGPEIRTHDMENG
ALELQAGNEIIVSTKQVLGTLGKFSVSYEGLADDVSAGSIILLDDGLIGLEVLESNPEKHEIKTKILNSGTLKNKKGVNV
PGVSVNLPGITEKDAKDIVFGIEQGVDFIAASFVRRSTDVLEIRELLEEHNASDIQIIPKIENQEGVDNLDAILEVSDGL
MVARGDLGVEIPAEEVPLVQKEMIKKCNALGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGQY
PVEAVQTMFNIATRTEESLNYKEILSKRRDQVGMTITDAIGQSVAHTAINLSAAAIVTPTESGHTARMIAKYRPQAPIVA
VTVNESVSRKLGLVFGVFPSSGQNANSTDEMLEDAVQKSLDSGIVKRGDLIVITAGSVGESGTTNLMKVHTVGSIIAKGQ
GIGRKSAFGPVVVAKNAKEAEQKMTDGAVLVASSTDRDMIASLEKASALITEEGGLTSHAAVVGLSLGIPVIVGLENATS
VLTEGQVITVDAARGAVYEGRASVL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI32967597, Length=493, Percent_Identity=44.0162271805274, Blast_Score=399, Evalue=1e-111,
Organism=Homo sapiens, GI10835121, Length=493, Percent_Identity=44.0162271805274, Blast_Score=399, Evalue=1e-111,
Organism=Homo sapiens, GI33286422, Length=488, Percent_Identity=44.2622950819672, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI33286420, Length=488, Percent_Identity=44.2622950819672, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI33286418, Length=488, Percent_Identity=44.2622950819672, Blast_Score=393, Evalue=1e-109,
Organism=Homo sapiens, GI310128732, Length=290, Percent_Identity=47.5862068965517, Blast_Score=248, Evalue=8e-66,
Organism=Homo sapiens, GI310128730, Length=290, Percent_Identity=47.5862068965517, Blast_Score=248, Evalue=8e-66,
Organism=Homo sapiens, GI310128736, Length=238, Percent_Identity=43.2773109243698, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI310128734, Length=238, Percent_Identity=43.2773109243698, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI310128738, Length=217, Percent_Identity=41.4746543778802, Blast_Score=156, Evalue=6e-38,
Organism=Escherichia coli, GI1787965, Length=474, Percent_Identity=53.3755274261603, Blast_Score=491, Evalue=1e-140,
Organism=Escherichia coli, GI1788160, Length=482, Percent_Identity=40.6639004149378, Blast_Score=304, Evalue=1e-83,
Organism=Escherichia coli, GI1787994, Length=107, Percent_Identity=41.1214953271028, Blast_Score=82, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17544584, Length=483, Percent_Identity=46.7908902691511, Blast_Score=400, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17506829, Length=489, Percent_Identity=44.9897750511247, Blast_Score=373, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI71984406, Length=489, Percent_Identity=44.9897750511247, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI71984413, Length=489, Percent_Identity=44.9897750511247, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17506831, Length=489, Percent_Identity=44.5807770961145, Blast_Score=372, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6319279, Length=485, Percent_Identity=43.0927835051546, Blast_Score=371, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6324923, Length=486, Percent_Identity=41.5637860082305, Blast_Score=354, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24648964, Length=486, Percent_Identity=46.0905349794239, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI28571814, Length=486, Percent_Identity=46.0905349794239, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24648966, Length=408, Percent_Identity=45.343137254902, Blast_Score=338, Evalue=8e-93,
Organism=Drosophila melanogaster, GI24581235, Length=481, Percent_Identity=34.0956340956341, Blast_Score=268, Evalue=6e-72,
Organism=Drosophila melanogaster, GI24646914, Length=266, Percent_Identity=44.3609022556391, Blast_Score=217, Evalue=2e-56,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 61866; Mature: 61866

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTKIVCTIGPASESVEMLTKLMEAGMNVARLNFSHGDFEEHGARIKNIREASKKLGKN
CCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
VGILLDTKGPEIRTHDMENGALELQAGNEIIVSTKQVLGTLGKFSVSYEGLADDVSAGSI
CEEEEECCCCCEEEEECCCCEEEEECCCEEEEECHHHHHHHHHCEEECCCCCCCCCCCEE
ILLDDGLIGLEVLESNPEKHEIKTKILNSGTLKNKKGVNVPGVSVNLPGITEKDAKDIVF
EEEECCCEEEHEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHH
GIEQGVDFIAASFVRRSTDVLEIRELLEEHNASDIQIIPKIENQEGVDNLDAILEVSDGL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHCCCE
MVARGDLGVEIPAEEVPLVQKEMIKKCNALGKPVITATQMLDSMQRNPRPTRAEASDVAN
EEEECCCCCCCCHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHH
AIFDGTDAIMLSGETAAGQYPVEAVQTMFNIATRTEESLNYKEILSKRRDQVGMTITDAI
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCHHCCCHHHHHHHHHHHCCCEEHHHH
GQSVAHTAINLSAAAIVTPTESGHTARMIAKYRPQAPIVAVTVNESVSRKLGLVFGVFPS
HHHHHHHHHEEEEEEEEECCCCCCHHHHHHHCCCCCCEEEEEECCHHHHCCCEEEEECCC
SGQNANSTDEMLEDAVQKSLDSGIVKRGDLIVITAGSVGESGTTNLMKVHTVGSIIAKGQ
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCEEEEEHHHHHHHCCC
GIGRKSAFGPVVVAKNAKEAEQKMTDGAVLVASSTDRDMIASLEKASALITEEGGLTSHA
CCCCCCCCCCEEEECCHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHEEECCCCCCHHH
AVVGLSLGIPVIVGLENATSVLTEGQVITVDAARGAVYEGRASVL
HHEEEECCCEEEEECCCHHHHHCCCEEEEEECCCCCEECCCCCCC
>Mature Secondary Structure
MKKTKIVCTIGPASESVEMLTKLMEAGMNVARLNFSHGDFEEHGARIKNIREASKKLGKN
CCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
VGILLDTKGPEIRTHDMENGALELQAGNEIIVSTKQVLGTLGKFSVSYEGLADDVSAGSI
CEEEEECCCCCEEEEECCCCEEEEECCCEEEEECHHHHHHHHHCEEECCCCCCCCCCCEE
ILLDDGLIGLEVLESNPEKHEIKTKILNSGTLKNKKGVNVPGVSVNLPGITEKDAKDIVF
EEEECCCEEEHEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHH
GIEQGVDFIAASFVRRSTDVLEIRELLEEHNASDIQIIPKIENQEGVDNLDAILEVSDGL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHCCCE
MVARGDLGVEIPAEEVPLVQKEMIKKCNALGKPVITATQMLDSMQRNPRPTRAEASDVAN
EEEECCCCCCCCHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHH
AIFDGTDAIMLSGETAAGQYPVEAVQTMFNIATRTEESLNYKEILSKRRDQVGMTITDAI
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCHHCCCHHHHHHHHHHHCCCEEHHHH
GQSVAHTAINLSAAAIVTPTESGHTARMIAKYRPQAPIVAVTVNESVSRKLGLVFGVFPS
HHHHHHHHHEEEEEEEEECCCCCCHHHHHHHCCCCCCEEEEEECCHHHHCCCEEEEECCC
SGQNANSTDEMLEDAVQKSLDSGIVKRGDLIVITAGSVGESGTTNLMKVHTVGSIIAKGQ
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCEEEEEHHHHHHHCCC
GIGRKSAFGPVVVAKNAKEAEQKMTDGAVLVASSTDRDMIASLEKASALITEEGGLTSHA
CCCCCCCCCCEEEECCHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHEEECCCCCCHHH
AVVGLSLGIPVIVGLENATSVLTEGQVITVDAARGAVYEGRASVL
HHEEEECCCEEEEECCCHHHHHCCCEEEEEECCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 9298659 [H]