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The map label for this gene is pheS [H]

Identifier: 154686999

GI number: 154686999

Start: 2693446

End: 2694483

Strand: Reverse

Name: pheS [H]

Synonym: RBAM_025710

Alternate gene names: 154686999

Gene position: 2694483-2693446 (Counterclockwise)

Preceding gene: 154687000

Following gene: 154686998

Centisome position: 68.76

GC content: 49.71

Gene sequence:

>1038_bases
ATGATGGAAGAGAAGCTTAAACAGCTTGAACTGGAAGCTGCAGAAAAAGTAGAAGCGGCGGGTTCATTAAAAGAAGTTAA
CGACATTCGGGTGCAATATCTCGGAAAAAAAGGGCCGATTACAGAAGTTCTCCGCGGCATGGGCAAACTGTCCGCCGAAG
AGCGCCCGAAAATGGGAGCGCTCGCCAATGAAGTGAGAGAGCGCATTGCAGCAGCCATTACGGCGAAAAATGAACAGCTT
GAACAAGAAGAAATGAATAAAAAACTCAGCAGCCAGACGATTGACGTCACATTGCCGGGCAGTCAGGTAAACATCGGCGG
ACGCCATCCGCTGACGGTCGTTATTGAAGAAATTGAAGATTTATTTATCGGCATGGGATATACCGTCGAAGAAGGCCCAG
AAGTGGAAACGGATTATTACAACTTTGAGGCGCTGAACCTGCCGAAGGAACACCCGGCCCGCGACATGCAGGACAGCTTT
TACATTACAGAAGAAATGCTGATGAGAACGCAGACGTCACCGGTGCAGACGCGCACGATGGAAAAGCATAAAGGAAAAGG
CCCCGTCAAAATCATTTGTCCCGGTAAAGTGTATCGCCGCGACAACGATGACGCGACACATTCCCATCAGTTCATGCAGA
TCGAGGGTCTCGTCGTCGACCGCAAAATCAGCATGAGCGACCTGAAAGGCACGCTTGAATTGGTCGCCAAAAAAATGTTC
GGCCAAGACCGTGAAATCAGGCTCCGTCCGAGCTTTTTCCCATTCACAGAGCCTTCTGTCGAGGTGGATGTGACCTGCTT
TAAGTGCGGCGGACAAGGCTGCTCCGTCTGCAAAAAAACAGGCTGGATCGAAATTCTCGGCGCCGGCATGGTTCACCCGA
ACGTGCTGAAGATGGCCGGATTCAATCCTGAGGAATACCAGGGTTTTGCTTTCGGAATGGGTGTTGAACGTATCGCGATG
CTGAAATACGGCATTGAAGATATCCGCCATTTCTATACCAATGATGTCAGATTCATTTCGCAGTTTAAACAGGCGTAA

Upstream 100 bases:

>100_bases
GTGGTACCGCGGCCACAACTCGTCCCTTCTCTCAAGGGGCGGGTTTTTTTGTTTTCTGAAAAAGGCATAGAACGGTTTGG
TTTACATAAAAGGAGGATGC

Downstream 100 bases:

>100_bases
GAGGAGGATACACATGTTTGTTTCTTATAAATGGTTAGAAGATTACGTTGACTTGCAGGGAATCGACCCGGCCGTGCTTG
CAGAAAAAATTACGAGAGCC

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MMEEKLKQLELEAAEKVEAAGSLKEVNDIRVQYLGKKGPITEVLRGMGKLSAEERPKMGALANEVRERIAAAITAKNEQL
EQEEMNKKLSSQTIDVTLPGSQVNIGGRHPLTVVIEEIEDLFIGMGYTVEEGPEVETDYYNFEALNLPKEHPARDMQDSF
YITEEMLMRTQTSPVQTRTMEKHKGKGPVKIICPGKVYRRDNDDATHSHQFMQIEGLVVDRKISMSDLKGTLELVAKKMF
GQDREIRLRPSFFPFTEPSVEVDVTCFKCGGQGCSVCKKTGWIEILGAGMVHPNVLKMAGFNPEEYQGFAFGMGVERIAM
LKYGIEDIRHFYTNDVRFISQFKQA

Sequences:

>Translated_345_residues
MMEEKLKQLELEAAEKVEAAGSLKEVNDIRVQYLGKKGPITEVLRGMGKLSAEERPKMGALANEVRERIAAAITAKNEQL
EQEEMNKKLSSQTIDVTLPGSQVNIGGRHPLTVVIEEIEDLFIGMGYTVEEGPEVETDYYNFEALNLPKEHPARDMQDSF
YITEEMLMRTQTSPVQTRTMEKHKGKGPVKIICPGKVYRRDNDDATHSHQFMQIEGLVVDRKISMSDLKGTLELVAKKMF
GQDREIRLRPSFFPFTEPSVEVDVTCFKCGGQGCSVCKKTGWIEILGAGMVHPNVLKMAGFNPEEYQGFAFGMGVERIAM
LKYGIEDIRHFYTNDVRFISQFKQA
>Mature_345_residues
MMEEKLKQLELEAAEKVEAAGSLKEVNDIRVQYLGKKGPITEVLRGMGKLSAEERPKMGALANEVRERIAAAITAKNEQL
EQEEMNKKLSSQTIDVTLPGSQVNIGGRHPLTVVIEEIEDLFIGMGYTVEEGPEVETDYYNFEALNLPKEHPARDMQDSF
YITEEMLMRTQTSPVQTRTMEKHKGKGPVKIICPGKVYRRDNDDATHSHQFMQIEGLVVDRKISMSDLKGTLELVAKKMF
GQDREIRLRPSFFPFTEPSVEVDVTCFKCGGQGCSVCKKTGWIEILGAGMVHPNVLKMAGFNPEEYQGFAFGMGVERIAM
LKYGIEDIRHFYTNDVRFISQFKQA

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=277, Percent_Identity=30.3249097472924, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI5729820, Length=291, Percent_Identity=27.8350515463918, Blast_Score=118, Evalue=8e-27,
Organism=Escherichia coli, GI1788007, Length=338, Percent_Identity=47.6331360946746, Blast_Score=340, Evalue=9e-95,
Organism=Caenorhabditis elegans, GI32566635, Length=254, Percent_Identity=33.0708661417323, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI32563657, Length=273, Percent_Identity=27.8388278388278, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17508957, Length=273, Percent_Identity=27.8388278388278, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6321087, Length=348, Percent_Identity=25.8620689655172, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6325304, Length=356, Percent_Identity=26.9662921348315, Blast_Score=111, Evalue=2e-25,
Organism=Drosophila melanogaster, GI18858079, Length=274, Percent_Identity=29.1970802919708, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI17137424, Length=303, Percent_Identity=27.0627062706271, Blast_Score=112, Evalue=3e-25,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 38939; Mature: 38939

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEEKLKQLELEAAEKVEAAGSLKEVNDIRVQYLGKKGPITEVLRGMGKLSAEERPKMGA
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEECCCCCCHHHHHHHHHHCCCCCCCCHHH
LANEVRERIAAAITAKNEQLEQEEMNKKLSSQTIDVTLPGSQVNIGGRHPLTVVIEEIED
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCEEEEEECCCEEECCCCCCHHHHHHHHHH
LFIGMGYTVEEGPEVETDYYNFEALNLPKEHPARDMQDSFYITEEMLMRTQTSPVQTRTM
HHHCCCCEECCCCCCCCCEECEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHH
EKHKGKGPVKIICPGKVYRRDNDDATHSHQFMQIEGLVVDRKISMSDLKGTLELVAKKMF
HHHCCCCCEEEEECCHHEECCCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHH
GQDREIRLRPSFFPFTEPSVEVDVTCFKCGGQGCSVCKKTGWIEILGAGMVHPNVLKMAG
CCCCEEEECCCCCCCCCCCCEEEEEEEEECCCCCHHHHHCCCEEEEECCCCCCCHHHHCC
FNPEEYQGFAFGMGVERIAMLKYGIEDIRHFYTNDVRFISQFKQA
CCHHHHCCEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MMEEKLKQLELEAAEKVEAAGSLKEVNDIRVQYLGKKGPITEVLRGMGKLSAEERPKMGA
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEECCCCCCHHHHHHHHHHCCCCCCCCHHH
LANEVRERIAAAITAKNEQLEQEEMNKKLSSQTIDVTLPGSQVNIGGRHPLTVVIEEIED
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCEEEEEECCCEEECCCCCCHHHHHHHHHH
LFIGMGYTVEEGPEVETDYYNFEALNLPKEHPARDMQDSFYITEEMLMRTQTSPVQTRTM
HHHCCCCEECCCCCCCCCEECEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHH
EKHKGKGPVKIICPGKVYRRDNDDATHSHQFMQIEGLVVDRKISMSDLKGTLELVAKKMF
HHHCCCCCEEEEECCHHEECCCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHH
GQDREIRLRPSFFPFTEPSVEVDVTCFKCGGQGCSVCKKTGWIEILGAGMVHPNVLKMAG
CCCCEEEECCCCCCCCCCCCEEEEEEEEECCCCCHHHHHCCCEEEEECCCCCCCHHHHCC
FNPEEYQGFAFGMGVERIAMLKYGIEDIRHFYTNDVRFISQFKQA
CCHHHHCCEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2127701; 1903307; 8969504; 9384377 [H]