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The map label for this gene is pheT [H]

Identifier: 154686998

GI number: 154686998

Start: 2691018

End: 2693432

Strand: Reverse

Name: pheT [H]

Synonym: RBAM_025700

Alternate gene names: 154686998

Gene position: 2693432-2691018 (Counterclockwise)

Preceding gene: 154686999

Following gene: 154686996

Centisome position: 68.73

GC content: 50.19

Gene sequence:

>2415_bases
ATGTTTGTTTCTTATAAATGGTTAGAAGATTACGTTGACTTGCAGGGAATCGACCCGGCCGTGCTTGCAGAAAAAATTAC
GAGAGCCGGTATTGAAGTTGAGGGTATCGAGTATAAAGGGGAGGGCGTAAAAGGCGTTGTCATCGGACACGTGCTTGAGC
GCGAACAGCACCCGAATGCCGACAAACTCAACAAATGCCTTGTTGATATTGGTGCGGAGGAACCCGTGCAGATTATCTGC
GGCGCCCCGAATGTTGATAAAGGACAGAAGGTTGCCGTTGCAACGGTCGGAGCCGTGCTTCCCGGCAATTTCAAAATTAA
AAAAGCCAAGCTGCGCGGCGAAGCGTCTCACGGCATGATCTGTTCCCTTCAGGAGCTCGGCATTGAAAGCAAGCTCGTGG
CGAAAGAATATGCGGAAGGCATTTTCGTTTTTCCGAATGATGCTGAAACGGGAGCTGACGCAATGGCGGCCCTTCAGCTT
GATGATGCGATTTTAGAATTGGGCTTAACGCCGAACCGCGCCGACGCGATGAACATGCTTGGCGTGGCTTATGAAGTTGC
GGCTATCCTCGGCGCAGAGGTAAAGCTTCCGGAGGCTTCATATGAAAAAGCGGCAGAAAAAGCGGCGGATGCTGTTTCCG
TTAAGGTTGAAGATACGCAGGCCAATCCGCATTATGCCGCAAAAATCATTAAAAACGTCAAAATCGGCCCGTCTCCGCTT
TGGATGCAGACGAAACTGATGAATGCAGGCATCCGTCCGCATAATAACGTCGTGGATATTACAAACTTCGTCCTCCTTGA
GTACGGACAGCCGCTTCACGCTTTCGATTACGACCGGTTCGGTTCAAAAGAGGTCGTCGTCAGAAAAGCGGCGGAAAACG
AAACCATCGTAACGCTGGATGAACAAGAGCGCAAGCTGTCAAGCAGCCATTTGGTCATCACAAACGGAAAAAAAGCGCAA
GCCGTTGCGGGAGTCATGGGCGGAGCTGAGTCTGAAGTCCGCGAAGACACCACTGCGATTTTACTGGAAGCCGCTTATTT
CGACGGACAGACCGTCCGCAAAGCGTCGAGAGACCTCGGATTGAGAAGCGAAGCGAGCGCCAGATATGAAAAAGGCATTG
ATCCGGCCCGCGTTTTGCTGGCGGCTGAACGGGCCTGCAGTCTCATTCAGGCATATGCCGGCGGAGAAGTGCTCTCAGGC
ACGGTTGAAGAAAACCATCTGAAAATCGAAGCAAACAACATTCACGTGTCTGTTGAAAAAGTGAACGCCGTACTCGGCAT
GACAATCGCTAAAGAAGAAATCATCAGCATTTACAAGCGCCTCGGGTTTGCAGTCGGTGAAGCTGAAGACGTTCTCGTCG
TAACGGTGCCGTCGCGCCGCGGAGATATTAGAATAGAAGAAGACCTGATTGAAGAAGCGGCGAGATTGTACGGGTATGAC
AATATCCCGTCCACGCTTCCGGAAACGGCCGGCACGACAGGCGGCTTGACACCGTATCAGGCAAAACGCCGGAAAGTGAG
ACGTTTCCTTGAAGGAGCGGGTCTTTCACAAGCGACCACTTATTCATTGACGAATGATAAAAAAGCGGCCGCTTTTGCGA
TCGAAAAATCATTCAACACGATGCTTGCTCTGCCGATGAGCGAAGAAAGAAGCATTCTCAGACACAGCCTCGTGCCGAAT
CTTCTTGAAGCCGTTTCATACAATCTGGCCAGACAGACTGATTCCGTTGCTTTATATGAAACGGGTTCTGTATTTCTGAC
GAAAGAAGAAAACACAAAACCGGTTGAAAAAGAACGGGTTGCCGGAGCTGTAACCGGATTGTGGCGCAAAAATTTATGGC
AGGGTGAGAAAAAGCCGGTTGATTTCTTCGTCGCCAAAGGAATCGTTGAAGGCCTGCTGCATCAATTGAATGTGTCAGAC
CGGGTTGAATTTGTCCAGTCAGAACGCAAAAACATGCATCCCGGCCGGACGGCAAACATTCTGTTGAACGGTTCGCTTGC
CGGATTTATCGGCCAGGTGCATCCGGCAATGGAAAAAGAGCTGGATATCAAAGAAACGTATGTGTTCGAGCTTGATCTGC
ACGCGCTTCTGACCGAGGAAACAGAACCTGTCGTGTATACGCCGATTCCGAAGTACCCGTCTGTCACGCGCGACATTGCG
CTTGTGGCAGATAAAACCGTTACAAGCGGACAGCTCGAAGCCGTGATCAAAGAAGCCGGCGGTGCGCTGCTGAAAGAAGT
CACCGTATTTGATGTGTACGAAGGTGAGCATATGGAAGAAGGCAAAAAATCCGTCGCCTTCTCGCTCCAATATGTTAACC
CTGAACAAACATTGACAGAAGAAGAAGTGACAAAAGTACATGAAAACGTGCTGAAAGCGCTGGAAGAAACGTATCAGGCC
GTATTAAGAGGATAA

Upstream 100 bases:

>100_bases
ATCGCGATGCTGAAATACGGCATTGAAGATATCCGCCATTTCTATACCAATGATGTCAGATTCATTTCGCAGTTTAAACA
GGCGTAAGAGGAGGATACAC

Downstream 100 bases:

>100_bases
AGAAAAAGCTTGCAGATCACTCTGCAAGCTTTTTTATTATGACCGCTTCCGTTCAACAAGGCGTTTCGCTTTTTGCGTAT
TGGCAAAATGAAGTTTGGTG

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 804; Mature: 804

Protein sequence:

>804_residues
MFVSYKWLEDYVDLQGIDPAVLAEKITRAGIEVEGIEYKGEGVKGVVIGHVLEREQHPNADKLNKCLVDIGAEEPVQIIC
GAPNVDKGQKVAVATVGAVLPGNFKIKKAKLRGEASHGMICSLQELGIESKLVAKEYAEGIFVFPNDAETGADAMAALQL
DDAILELGLTPNRADAMNMLGVAYEVAAILGAEVKLPEASYEKAAEKAADAVSVKVEDTQANPHYAAKIIKNVKIGPSPL
WMQTKLMNAGIRPHNNVVDITNFVLLEYGQPLHAFDYDRFGSKEVVVRKAAENETIVTLDEQERKLSSSHLVITNGKKAQ
AVAGVMGGAESEVREDTTAILLEAAYFDGQTVRKASRDLGLRSEASARYEKGIDPARVLLAAERACSLIQAYAGGEVLSG
TVEENHLKIEANNIHVSVEKVNAVLGMTIAKEEIISIYKRLGFAVGEAEDVLVVTVPSRRGDIRIEEDLIEEAARLYGYD
NIPSTLPETAGTTGGLTPYQAKRRKVRRFLEGAGLSQATTYSLTNDKKAAAFAIEKSFNTMLALPMSEERSILRHSLVPN
LLEAVSYNLARQTDSVALYETGSVFLTKEENTKPVEKERVAGAVTGLWRKNLWQGEKKPVDFFVAKGIVEGLLHQLNVSD
RVEFVQSERKNMHPGRTANILLNGSLAGFIGQVHPAMEKELDIKETYVFELDLHALLTEETEPVVYTPIPKYPSVTRDIA
LVADKTVTSGQLEAVIKEAGGALLKEVTVFDVYEGEHMEEGKKSVAFSLQYVNPEQTLTEEEVTKVHENVLKALEETYQA
VLRG

Sequences:

>Translated_804_residues
MFVSYKWLEDYVDLQGIDPAVLAEKITRAGIEVEGIEYKGEGVKGVVIGHVLEREQHPNADKLNKCLVDIGAEEPVQIIC
GAPNVDKGQKVAVATVGAVLPGNFKIKKAKLRGEASHGMICSLQELGIESKLVAKEYAEGIFVFPNDAETGADAMAALQL
DDAILELGLTPNRADAMNMLGVAYEVAAILGAEVKLPEASYEKAAEKAADAVSVKVEDTQANPHYAAKIIKNVKIGPSPL
WMQTKLMNAGIRPHNNVVDITNFVLLEYGQPLHAFDYDRFGSKEVVVRKAAENETIVTLDEQERKLSSSHLVITNGKKAQ
AVAGVMGGAESEVREDTTAILLEAAYFDGQTVRKASRDLGLRSEASARYEKGIDPARVLLAAERACSLIQAYAGGEVLSG
TVEENHLKIEANNIHVSVEKVNAVLGMTIAKEEIISIYKRLGFAVGEAEDVLVVTVPSRRGDIRIEEDLIEEAARLYGYD
NIPSTLPETAGTTGGLTPYQAKRRKVRRFLEGAGLSQATTYSLTNDKKAAAFAIEKSFNTMLALPMSEERSILRHSLVPN
LLEAVSYNLARQTDSVALYETGSVFLTKEENTKPVEKERVAGAVTGLWRKNLWQGEKKPVDFFVAKGIVEGLLHQLNVSD
RVEFVQSERKNMHPGRTANILLNGSLAGFIGQVHPAMEKELDIKETYVFELDLHALLTEETEPVVYTPIPKYPSVTRDIA
LVADKTVTSGQLEAVIKEAGGALLKEVTVFDVYEGEHMEEGKKSVAFSLQYVNPEQTLTEEEVTKVHENVLKALEETYQA
VLRG
>Mature_804_residues
MFVSYKWLEDYVDLQGIDPAVLAEKITRAGIEVEGIEYKGEGVKGVVIGHVLEREQHPNADKLNKCLVDIGAEEPVQIIC
GAPNVDKGQKVAVATVGAVLPGNFKIKKAKLRGEASHGMICSLQELGIESKLVAKEYAEGIFVFPNDAETGADAMAALQL
DDAILELGLTPNRADAMNMLGVAYEVAAILGAEVKLPEASYEKAAEKAADAVSVKVEDTQANPHYAAKIIKNVKIGPSPL
WMQTKLMNAGIRPHNNVVDITNFVLLEYGQPLHAFDYDRFGSKEVVVRKAAENETIVTLDEQERKLSSSHLVITNGKKAQ
AVAGVMGGAESEVREDTTAILLEAAYFDGQTVRKASRDLGLRSEASARYEKGIDPARVLLAAERACSLIQAYAGGEVLSG
TVEENHLKIEANNIHVSVEKVNAVLGMTIAKEEIISIYKRLGFAVGEAEDVLVVTVPSRRGDIRIEEDLIEEAARLYGYD
NIPSTLPETAGTTGGLTPYQAKRRKVRRFLEGAGLSQATTYSLTNDKKAAAFAIEKSFNTMLALPMSEERSILRHSLVPN
LLEAVSYNLARQTDSVALYETGSVFLTKEENTKPVEKERVAGAVTGLWRKNLWQGEKKPVDFFVAKGIVEGLLHQLNVSD
RVEFVQSERKNMHPGRTANILLNGSLAGFIGQVHPAMEKELDIKETYVFELDLHALLTEETEPVVYTPIPKYPSVTRDIA
LVADKTVTSGQLEAVIKEAGGALLKEVTVFDVYEGEHMEEGKKSVAFSLQYVNPEQTLTEEEVTKVHENVLKALEETYQA
VLRG

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI124028525, Length=271, Percent_Identity=25.0922509225092, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI1788006, Length=809, Percent_Identity=39.0605686032138, Blast_Score=498, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI17533403, Length=565, Percent_Identity=20, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21355211, Length=569, Percent_Identity=21.2653778558875, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 87807; Mature: 87807

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVSYKWLEDYVDLQGIDPAVLAEKITRAGIEVEGIEYKGEGVKGVVIGHVLEREQHPNA
CCEEHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCEEEHHHHHHHCCCCCH
DKLNKCLVDIGAEEPVQIICGAPNVDKGQKVAVATVGAVLPGNFKIKKAKLRGEASHGMI
HHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEEEECEECCCCEEEEEEEECCCCCCCEE
CSLQELGIESKLVAKEYAEGIFVFPNDAETGADAMAALQLDDAILELGLTPNRADAMNML
EEHHHCCCCHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
GVAYEVAAILGAEVKLPEASYEKAAEKAADAVSVKVEDTQANPHYAAKIIKNVKIGPSPL
HHHHHHHHHHCCCEECCCHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCC
WMQTKLMNAGIRPHNNVVDITNFVLLEYGQPLHAFDYDRFGSKEVVVRKAAENETIVTLD
HHHHHHHHCCCCCCCCEEHHHHEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEC
EQERKLSSSHLVITNGKKAQAVAGVMGGAESEVREDTTAILLEAAYFDGQTVRKASRDLG
HHHHHCCCCEEEEECCCCHHHHHHHHCCCHHHHHHCHHEEEEEEHHCCCHHHHHHHHHCC
LRSEASARYEKGIDPARVLLAAERACSLIQAYAGGEVLSGTVEENHLKIEANNIHVSVEK
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEECCEECCEEEEEECCEEEEHHH
VNAVLGMTIAKEEIISIYKRLGFAVGEAEDVLVVTVPSRRGDIRIEEDLIEEAARLYGYD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEEHHHHHHHHHHHCCCC
NIPSTLPETAGTTGGLTPYQAKRRKVRRFLEGAGLSQATTYSLTNDKKAAAFAIEKSFNT
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHEEEECCCCE
MLALPMSEERSILRHSLVPNLLEAVSYNLARQTDSVALYETGSVFLTKEENTKPVEKERV
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEECCCCCCHHHHHH
AGAVTGLWRKNLWQGEKKPVDFFVAKGIVEGLLHQLNVSDRVEFVQSERKNMHPGRTANI
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEE
LLNGSLAGFIGQVHPAMEKELDIKETYVFELDLHALLTEETEPVVYTPIPKYPSVTRDIA
EEECCHHHHHHHHCHHHHHCCCCCEEEEEEEEHHHHHCCCCCCEEEECCCCCCCCHHCEE
LVADKTVTSGQLEAVIKEAGGALLKEVTVFDVYEGEHMEEGKKSVAFSLQYVNPEQTLTE
EEECCCCCCHHHHHHHHHHCCHHHHHEEEEEEECCCHHHCCCCEEEEEEEEECCHHHCCH
EEVTKVHENVLKALEETYQAVLRG
HHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFVSYKWLEDYVDLQGIDPAVLAEKITRAGIEVEGIEYKGEGVKGVVIGHVLEREQHPNA
CCEEHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCEEEHHHHHHHCCCCCH
DKLNKCLVDIGAEEPVQIICGAPNVDKGQKVAVATVGAVLPGNFKIKKAKLRGEASHGMI
HHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEEEECEECCCCEEEEEEEECCCCCCCEE
CSLQELGIESKLVAKEYAEGIFVFPNDAETGADAMAALQLDDAILELGLTPNRADAMNML
EEHHHCCCCHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
GVAYEVAAILGAEVKLPEASYEKAAEKAADAVSVKVEDTQANPHYAAKIIKNVKIGPSPL
HHHHHHHHHHCCCEECCCHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCC
WMQTKLMNAGIRPHNNVVDITNFVLLEYGQPLHAFDYDRFGSKEVVVRKAAENETIVTLD
HHHHHHHHCCCCCCCCEEHHHHEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEC
EQERKLSSSHLVITNGKKAQAVAGVMGGAESEVREDTTAILLEAAYFDGQTVRKASRDLG
HHHHHCCCCEEEEECCCCHHHHHHHHCCCHHHHHHCHHEEEEEEHHCCCHHHHHHHHHCC
LRSEASARYEKGIDPARVLLAAERACSLIQAYAGGEVLSGTVEENHLKIEANNIHVSVEK
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEECCEECCEEEEEECCEEEEHHH
VNAVLGMTIAKEEIISIYKRLGFAVGEAEDVLVVTVPSRRGDIRIEEDLIEEAARLYGYD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEEHHHHHHHHHHHCCCC
NIPSTLPETAGTTGGLTPYQAKRRKVRRFLEGAGLSQATTYSLTNDKKAAAFAIEKSFNT
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHEEEECCCCE
MLALPMSEERSILRHSLVPNLLEAVSYNLARQTDSVALYETGSVFLTKEENTKPVEKERV
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEECCCCCCHHHHHH
AGAVTGLWRKNLWQGEKKPVDFFVAKGIVEGLLHQLNVSDRVEFVQSERKNMHPGRTANI
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEE
LLNGSLAGFIGQVHPAMEKELDIKETYVFELDLHALLTEETEPVVYTPIPKYPSVTRDIA
EEECCHHHHHHHHCHHHHHCCCCCEEEEEEEEHHHHHCCCCCCEEEECCCCCCCCHHCEE
LVADKTVTSGQLEAVIKEAGGALLKEVTVFDVYEGEHMEEGKKSVAFSLQYVNPEQTLTE
EEECCCCCCHHHHHHHHHHCCHHHHHEEEEEEECCCHHHCCCCEEEEEEEEECCHHHCCH
EEVTKVHENVLKALEETYQAVLRG
HHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2127701; 1903307; 8969504; 9384377 [H]