Click here to switch to the map view.

The map label for this gene is lysC [H]

Identifier: 154686982

GI number: 154686982

Start: 2672939

End: 2674168

Strand: Reverse

Name: lysC [H]

Synonym: RBAM_025540

Alternate gene names: 154686982

Gene position: 2674168-2672939 (Counterclockwise)

Preceding gene: 154686983

Following gene: 154686980

Centisome position: 68.24

GC content: 49.84

Gene sequence:

>1230_bases
ATGGGTCTTATCGTACAAAAATTCGGAGGCACTTCCGTCGGCTCTGCCGAAAAAATTCAAAATGCCGCAAATCGCGCCAT
TGCTGAAAAGCAAAAAGGCCATGATGTCGTAGTCGTCGTTTCGGCAATGGGAAAATCAACCGACGCGCTCGTTAACCTCG
CGGCTGAAATCACATCTGAACCAAGCAGACGCGAGATGGATATGCTTTTGTCTACGGGAGAGCAGGTCACGATTTCGCTT
TTGGCCATGGCGCTTCAGGAAAAAGGATATGACGCCGTCTCTTATACGGGCTGGCAGGCGGGTGTCCGCACGGAAGCTGT
TCACGGCAATGCGAGAATTACGGATATTGATATCACGGCGATTCGTTCGCAGCTTGCAGAAGGCAGGATTACAGTCGTTG
CCGGTTTTCAGGGCGTAACGGAAGACGGAGGCATCACGACGCTCGGACGGGGCGGTTCCGATACGACGGCTGTCGCTCTT
GCCGCAGCGCTGGAAGCGGATAAATGCGACATTTATACAGATGTTCCGGGCGTATTTACGACAGATCCGCGGTATGTCCG
GTCAGCGAGAAAACTGGCGGGAATTTCTTATGATGAAATGCTGGAGCTTGCAAATCTGGGAGCGGGTGTTCTTCACCCGC
GGGCGGTTGAGTTCGCGAAAAACTACCAAGTGCCTTTGGAAGTGCGGTCAAGCACGGAACATGAAGCGGGAACATTAATT
GAGGAGGAATCATCAATGGAGCAGAATTTAATCGTCAGAGGAATCGCATTTGAAGACCAGATCACGAGGGTGTCCGTTAC
AGGCCTTGCAAGCGGATTGACAACACTTTCAACAATCTTCACCGCGCTGGCAAAACGAAACATCAATGTTGACATTATTA
TTCAGACACAGTCGGACGATGGGGAAGCGGATATTTCTTTCTCAGTGAAAACGGAAGATGCCGCGCAAACGGTCGCCGTG
CTTGAAGAATATAAAAACGCGCTTGACTATGAAAAAATTGAAACCGAAAGAAAACTGGCGAAAGTCTCTATTGTCGGGTC
GGGCATGGTTTCAAATCCCGGCGTCGCCGCGGACATGTTTGCCGTACTGGCGGAAAAAAATGTACAAGTCAAAATGGTGA
GCACATCTGAAATAAAAGTATCAGCGGTTGTCAGCGAAAATGATATGGTGAAAGCGGTCGAAGCGCTTCATAATGCGTTT
GAGCTTTCTAAACAGCCGTCTGCCGTGTAA

Upstream 100 bases:

>100_bases
AAAAGCAATGAGAGCTATTCCTCTCATTGCTTTTTTTATTGTGGACAAAACGCTCTTTTCTCCTCACCCGCACGAACCAA
AATGTAAAGGGTGGTCTAGT

Downstream 100 bases:

>100_bases
ACGGGCAGGCAAAAAGGCGGGACTTGGAGTCACCGCCTTTTTTCAATTATACAGGGTCCTTCATATCCCATTTTACAGTC
AGGACGACTTTATCCGCTCT

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase 2; Aspartokinase II [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MGLIVQKFGGTSVGSAEKIQNAANRAIAEKQKGHDVVVVVSAMGKSTDALVNLAAEITSEPSRREMDMLLSTGEQVTISL
LAMALQEKGYDAVSYTGWQAGVRTEAVHGNARITDIDITAIRSQLAEGRITVVAGFQGVTEDGGITTLGRGGSDTTAVAL
AAALEADKCDIYTDVPGVFTTDPRYVRSARKLAGISYDEMLELANLGAGVLHPRAVEFAKNYQVPLEVRSSTEHEAGTLI
EEESSMEQNLIVRGIAFEDQITRVSVTGLASGLTTLSTIFTALAKRNINVDIIIQTQSDDGEADISFSVKTEDAAQTVAV
LEEYKNALDYEKIETERKLAKVSIVGSGMVSNPGVAADMFAVLAEKNVQVKMVSTSEIKVSAVVSENDMVKAVEALHNAF
ELSKQPSAV

Sequences:

>Translated_409_residues
MGLIVQKFGGTSVGSAEKIQNAANRAIAEKQKGHDVVVVVSAMGKSTDALVNLAAEITSEPSRREMDMLLSTGEQVTISL
LAMALQEKGYDAVSYTGWQAGVRTEAVHGNARITDIDITAIRSQLAEGRITVVAGFQGVTEDGGITTLGRGGSDTTAVAL
AAALEADKCDIYTDVPGVFTTDPRYVRSARKLAGISYDEMLELANLGAGVLHPRAVEFAKNYQVPLEVRSSTEHEAGTLI
EEESSMEQNLIVRGIAFEDQITRVSVTGLASGLTTLSTIFTALAKRNINVDIIIQTQSDDGEADISFSVKTEDAAQTVAV
LEEYKNALDYEKIETERKLAKVSIVGSGMVSNPGVAADMFAVLAEKNVQVKMVSTSEIKVSAVVSENDMVKAVEALHNAF
ELSKQPSAV
>Mature_408_residues
GLIVQKFGGTSVGSAEKIQNAANRAIAEKQKGHDVVVVVSAMGKSTDALVNLAAEITSEPSRREMDMLLSTGEQVTISLL
AMALQEKGYDAVSYTGWQAGVRTEAVHGNARITDIDITAIRSQLAEGRITVVAGFQGVTEDGGITTLGRGGSDTTAVALA
AALEADKCDIYTDVPGVFTTDPRYVRSARKLAGISYDEMLELANLGAGVLHPRAVEFAKNYQVPLEVRSSTEHEAGTLIE
EESSMEQNLIVRGIAFEDQITRVSVTGLASGLTTLSTIFTALAKRNINVDIIIQTQSDDGEADISFSVKTEDAAQTVAVL
EEYKNALDYEKIETERKLAKVSIVGSGMVSNPGVAADMFAVLAEKNVQVKMVSTSEIKVSAVVSENDMVKAVEALHNAFE
LSKQPSAV

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=343, Percent_Identity=33.8192419825073, Blast_Score=164, Evalue=8e-42,
Organism=Escherichia coli, GI1790455, Length=363, Percent_Identity=31.6804407713499, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1790376, Length=295, Percent_Identity=25.0847457627119, Blast_Score=76, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6320893, Length=378, Percent_Identity=26.7195767195767, Blast_Score=122, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 43477; Mature: 43345

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLIVQKFGGTSVGSAEKIQNAANRAIAEKQKGHDVVVVVSAMGKSTDALVNLAAEITSE
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCC
PSRREMDMLLSTGEQVTISLLAMALQEKGYDAVSYTGWQAGVRTEAVHGNARITDIDITA
CCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCEEEEEHHHH
IRSQLAEGRITVVAGFQGVTEDGGITTLGRGGSDTTAVALAAALEADKCDIYTDVPGVFT
HHHHHCCCCEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCEEEECCCCCEE
TDPRYVRSARKLAGISYDEMLELANLGAGVLHPRAVEFAKNYQVPLEVRSSTEHEAGTLI
CCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCEE
EEESSMEQNLIVRGIAFEDQITRVSVTGLASGLTTLSTIFTALAKRNINVDIIIQTQSDD
CCHHHHCCCEEEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
GEADISFSVKTEDAAQTVAVLEEYKNALDYEKIETERKLAKVSIVGSGMVSNPGVAADMF
CCCEEEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHEEEEEEECCCCCCCCHHHHHH
AVLAEKNVQVKMVSTSEIKVSAVVSENDMVKAVEALHNAFELSKQPSAV
HHHHCCCCEEEEEECCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GLIVQKFGGTSVGSAEKIQNAANRAIAEKQKGHDVVVVVSAMGKSTDALVNLAAEITSE
CEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCC
PSRREMDMLLSTGEQVTISLLAMALQEKGYDAVSYTGWQAGVRTEAVHGNARITDIDITA
CCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCEEEEEHHHH
IRSQLAEGRITVVAGFQGVTEDGGITTLGRGGSDTTAVALAAALEADKCDIYTDVPGVFT
HHHHHCCCCEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCEEEECCCCCEE
TDPRYVRSARKLAGISYDEMLELANLGAGVLHPRAVEFAKNYQVPLEVRSSTEHEAGTLI
CCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCEE
EEESSMEQNLIVRGIAFEDQITRVSVTGLASGLTTLSTIFTALAKRNINVDIIIQTQSDD
CCHHHHCCCEEEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
GEADISFSVKTEDAAQTVAVLEEYKNALDYEKIETERKLAKVSIVGSGMVSNPGVAADMF
CCCEEEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHEEEEEEECCCCCCCCHHHHHH
AVLAEKNVQVKMVSTSEIKVSAVVSENDMVKAVEALHNAFELSKQPSAV
HHHHCCCCEEEEEECCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036830; 8969504; 9384377; 2837491; 2168395 [H]