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The map label for this gene is ilvB [H]

Identifier: 154686967

GI number: 154686967

Start: 2659791

End: 2661584

Strand: Reverse

Name: ilvB [H]

Synonym: RBAM_025370

Alternate gene names: 154686967

Gene position: 2661584-2659791 (Counterclockwise)

Preceding gene: 154686968

Following gene: 154686966

Centisome position: 67.92

GC content: 50.56

Gene sequence:

>1794_bases
TTGCGTCAGCCGGCGCTGCGTTTTATGAATTGGATTTTAGAAGGGAACATTGAAAAGGAGGAACTGAAAATGGGCACAAA
TGTACAGGCGGATTCTTTGTCTGCCGAATGTACAAAAACAATGAACGGGGCTCTGATGCTGATTGAATCATTAAAGAAAG
AAAATGTCGAGATGATCTTCGGCTATCCGGGGGGAGCCGTCCTTCCGATTTACGATAAACTGTATCAATCAGGGCTGGTC
CACATCCTGCCCCGCCACGAGCAAGGCGCGATTCACGCGGCAGAAGGGTATGCGCGAATCTCCGGAAAGCCGGGGGTTGT
GATTGCTACCTCCGGACCCGGAGCGACAAATTTAGTTACCGGTCTTGCGGATGCGATGATAGATTCATTGCCGCTCGTAG
TGTTTACCGGTCAGGTGGCCACTTCGGTCATCGGCAGCGACGCTTTCCAGGAAGCCGACATTTTAGGAATTACGATGCCG
ATTACGAAACACAGCTACCAAGTCCGCCGTCCGGAAGATCTCCCCCGTGTGATTAAAGAGGCGTTCCATATCGCCACGAC
GGGAAGACCGGGCCCGGTATTAATTGATATACCGAAGGATGTCGCAGCATTTGAAGGAGAGTTCAGATATGATCACGAGA
TCAGCCTTCCGGGTTATCAGCCGGTAAAAGAGCCTAACTATCTGCAGATCCGAAAGCTCGTGGAAGCTGTCAGCAGTGCG
AAAAAGCCGGTTATTTTAGCCGGAGCGGGTGTTTTGCACGGTAAAGCTTCAGAAGATTTAAAAAATTATGCCGAACAGCA
GCAAATACCGGTAGCGCACACGCTTCTCGGTCTGGGCGGTTTCCCGGCAGACCATCCTCTTTTTCTGGGAATGGCGGGGA
TGCACGGCACATACACAGCGAATATGGCGCTTTACCACTGTGACTTGTTGATCAGCATCGGAGCGCGGTTCGATGATCGC
GTGACCGGAAATCTGAAGCATTTTGCAAAATCGGCCAAAGTGGCGCATATCGATATTGATCCTGCTGAAATCGGAAAAAT
TATTGAAACGCAGATTCCGGTGGTCGGCGACAGTAAAATCGTCCTTCAGGAATTATTGAAGCAAAACGGAAAACAGGGAC
AGACAGAGGAATGGAAACAGCAGCTTTCAGAATGGAAAGAAGAATATCCGCTTTGGTATACAGACAACAGAGAAGAGGGC
TTGAAACCCCAAAAGCTGATTGAGTATATTCACCAATTCACAAACGGTGAGGCGATTGTGGCGACGGATGTCGGCCAGCA
CCAGATGTGGGCGGCTCAGTTTTATCCTTTCCGAAAGGCTGATAAGTGGGTTACCTCGGGGGGACTCGGCACAATGGGCT
TCGGCCTTCCGGCGGCTATCGGAGCGCAGCTGGCCGATCGTAACGCCACGGTCGTTGCCATCCTCGGAGACGGCGGTTTT
CAGATGACCCTTCAGGAATTAGATGTCATCCGGCAGCTGAATCTTCCTGTCAAAGTGGTTATCTTGAACAACGAATGTCT
GGGCATGGTCAGACAGTGGCAGGAAATCTTCTATGAAGAGCGCTATTCTGAATCCAAATTCTCGGCGCAGCCTGACTTTG
TCAAATTGTCAGAAGCCTACGGCATTAAAGGCGTCCGGATTTCATCGGAGGAAGAGGCGGAAGAAGAGCTTAAAAAGGCG
CTTTCATCAAAAGAACCGGCCGTTATTGACGTCCGGGTCGCCAAAAGCGAGAAAGTATTCCCGATGATTGCGCCGGGTAA
AGGACTTCATGAAATGGTGGGGGTGAAACCTTGA

Upstream 100 bases:

>100_bases
ACCGTAAACAAGGGTGGTACCGCGGAGAGCAAAGCCTTTTCGCCCCTTTTGGCTATCGCATGAAATGCTTGGCTGAATGT
GGGTGGGAAGGCTTTTTTAT

Downstream 100 bases:

>100_bases
AACGCATTATCACATTAACGGTGGTCAACCGCTCGGGAGTATTAAACCGGATCACCGGCCTGTTTACAAAAAGACACTAT
AATATCGAAAGCATCACTGT

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 597; Mature: 597

Protein sequence:

>597_residues
MRQPALRFMNWILEGNIEKEELKMGTNVQADSLSAECTKTMNGALMLIESLKKENVEMIFGYPGGAVLPIYDKLYQSGLV
HILPRHEQGAIHAAEGYARISGKPGVVIATSGPGATNLVTGLADAMIDSLPLVVFTGQVATSVIGSDAFQEADILGITMP
ITKHSYQVRRPEDLPRVIKEAFHIATTGRPGPVLIDIPKDVAAFEGEFRYDHEISLPGYQPVKEPNYLQIRKLVEAVSSA
KKPVILAGAGVLHGKASEDLKNYAEQQQIPVAHTLLGLGGFPADHPLFLGMAGMHGTYTANMALYHCDLLISIGARFDDR
VTGNLKHFAKSAKVAHIDIDPAEIGKIIETQIPVVGDSKIVLQELLKQNGKQGQTEEWKQQLSEWKEEYPLWYTDNREEG
LKPQKLIEYIHQFTNGEAIVATDVGQHQMWAAQFYPFRKADKWVTSGGLGTMGFGLPAAIGAQLADRNATVVAILGDGGF
QMTLQELDVIRQLNLPVKVVILNNECLGMVRQWQEIFYEERYSESKFSAQPDFVKLSEAYGIKGVRISSEEEAEEELKKA
LSSKEPAVIDVRVAKSEKVFPMIAPGKGLHEMVGVKP

Sequences:

>Translated_597_residues
MRQPALRFMNWILEGNIEKEELKMGTNVQADSLSAECTKTMNGALMLIESLKKENVEMIFGYPGGAVLPIYDKLYQSGLV
HILPRHEQGAIHAAEGYARISGKPGVVIATSGPGATNLVTGLADAMIDSLPLVVFTGQVATSVIGSDAFQEADILGITMP
ITKHSYQVRRPEDLPRVIKEAFHIATTGRPGPVLIDIPKDVAAFEGEFRYDHEISLPGYQPVKEPNYLQIRKLVEAVSSA
KKPVILAGAGVLHGKASEDLKNYAEQQQIPVAHTLLGLGGFPADHPLFLGMAGMHGTYTANMALYHCDLLISIGARFDDR
VTGNLKHFAKSAKVAHIDIDPAEIGKIIETQIPVVGDSKIVLQELLKQNGKQGQTEEWKQQLSEWKEEYPLWYTDNREEG
LKPQKLIEYIHQFTNGEAIVATDVGQHQMWAAQFYPFRKADKWVTSGGLGTMGFGLPAAIGAQLADRNATVVAILGDGGF
QMTLQELDVIRQLNLPVKVVILNNECLGMVRQWQEIFYEERYSESKFSAQPDFVKLSEAYGIKGVRISSEEEAEEELKKA
LSSKEPAVIDVRVAKSEKVFPMIAPGKGLHEMVGVKP
>Mature_597_residues
MRQPALRFMNWILEGNIEKEELKMGTNVQADSLSAECTKTMNGALMLIESLKKENVEMIFGYPGGAVLPIYDKLYQSGLV
HILPRHEQGAIHAAEGYARISGKPGVVIATSGPGATNLVTGLADAMIDSLPLVVFTGQVATSVIGSDAFQEADILGITMP
ITKHSYQVRRPEDLPRVIKEAFHIATTGRPGPVLIDIPKDVAAFEGEFRYDHEISLPGYQPVKEPNYLQIRKLVEAVSSA
KKPVILAGAGVLHGKASEDLKNYAEQQQIPVAHTLLGLGGFPADHPLFLGMAGMHGTYTANMALYHCDLLISIGARFDDR
VTGNLKHFAKSAKVAHIDIDPAEIGKIIETQIPVVGDSKIVLQELLKQNGKQGQTEEWKQQLSEWKEEYPLWYTDNREEG
LKPQKLIEYIHQFTNGEAIVATDVGQHQMWAAQFYPFRKADKWVTSGGLGTMGFGLPAAIGAQLADRNATVVAILGDGGF
QMTLQELDVIRQLNLPVKVVILNNECLGMVRQWQEIFYEERYSESKFSAQPDFVKLSEAYGIKGVRISSEEEAEEELKKA
LSSKEPAVIDVRVAKSEKVFPMIAPGKGLHEMVGVKP

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=565, Percent_Identity=24.4247787610619, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI21361361, Length=583, Percent_Identity=24.6998284734134, Blast_Score=162, Evalue=1e-39,
Organism=Escherichia coli, GI87081685, Length=564, Percent_Identity=47.1631205673759, Blast_Score=541, Evalue=1e-155,
Organism=Escherichia coli, GI1790104, Length=568, Percent_Identity=43.3098591549296, Blast_Score=472, Evalue=1e-134,
Organism=Escherichia coli, GI1786717, Length=543, Percent_Identity=35.7274401473296, Blast_Score=327, Evalue=2e-90,
Organism=Escherichia coli, GI1787096, Length=544, Percent_Identity=29.2279411764706, Blast_Score=217, Evalue=2e-57,
Organism=Escherichia coli, GI1788716, Length=548, Percent_Identity=25.1824817518248, Blast_Score=170, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17531299, Length=595, Percent_Identity=27.2268907563025, Blast_Score=181, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17531301, Length=595, Percent_Identity=27.2268907563025, Blast_Score=181, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17542570, Length=606, Percent_Identity=24.4224422442244, Blast_Score=151, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6323755, Length=579, Percent_Identity=46.9775474956822, Blast_Score=515, Evalue=1e-147,
Organism=Saccharomyces cerevisiae, GI6320816, Length=552, Percent_Identity=22.463768115942, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6323163, Length=486, Percent_Identity=25.3086419753086, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321524, Length=491, Percent_Identity=25.050916496945, Blast_Score=97, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6323073, Length=493, Percent_Identity=24.7464503042596, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19922626, Length=564, Percent_Identity=24.113475177305, Blast_Score=171, Evalue=2e-42,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 65620; Mature: 65620

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQPALRFMNWILEGNIEKEELKMGTNVQADSLSAECTKTMNGALMLIESLKKENVEMIF
CCCHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHCCCEEEE
GYPGGAVLPIYDKLYQSGLVHILPRHEQGAIHAAEGYARISGKPGVVIATSGPGATNLVT
ECCCCCEEHHHHHHHHCCCEEEEECCCCCCEEECCCEEEECCCCCEEEEECCCCHHHHHH
GLADAMIDSLPLVVFTGQVATSVIGSDAFQEADILGITMPITKHSYQVRRPEDLPRVIKE
HHHHHHHHCCCEEEEECHHHHHHHCCCHHCCCCEEEEEECCCCCCEECCCCHHHHHHHHH
AFHIATTGRPGPVLIDIPKDVAAFEGEFRYDHEISLPGYQPVKEPNYLQIRKLVEAVSSA
HHHEEECCCCCCEEEECCCHHHHCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHC
KKPVILAGAGVLHGKASEDLKNYAEQQQIPVAHTLLGLGGFPADHPLFLGMAGMHGTYTA
CCCEEEECCCEECCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEEECCCCCCEEC
NMALYHCDLLISIGARFDDRVTGNLKHFAKSAKVAHIDIDPAEIGKIIETQIPVVGDSKI
CCCEEEHHHHEECCCCCCCCCCCCHHHHHHCCCEEEEECCHHHHHHHHHHCCCCCCCCHH
VLQELLKQNGKQGQTEEWKQQLSEWKEEYPLWYTDNREEGLKPQKLIEYIHQFTNGEAIV
HHHHHHHHCCCCCCHHHHHHHHHHHHHHCCEEEECCHHHCCCHHHHHHHHHHHCCCCEEE
ATDVGQHQMWAAQFYPFRKADKWVTSGGLGTMGFGLPAAIGAQLADRNATVVAILGDGGF
EECCCCHHHHHHHHCCCHHHCCEECCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCC
QMTLQELDVIRQLNLPVKVVILNNECLGMVRQWQEIFYEERYSESKFSAQPDFVKLSEAY
EEHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHC
GIKGVRISSEEEAEEELKKALSSKEPAVIDVRVAKSEKVFPMIAPGKGLHEMVGVKP
CCCEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEECCCCCHHHHCCCCC
>Mature Secondary Structure
MRQPALRFMNWILEGNIEKEELKMGTNVQADSLSAECTKTMNGALMLIESLKKENVEMIF
CCCHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHCCCEEEE
GYPGGAVLPIYDKLYQSGLVHILPRHEQGAIHAAEGYARISGKPGVVIATSGPGATNLVT
ECCCCCEEHHHHHHHHCCCEEEEECCCCCCEEECCCEEEECCCCCEEEEECCCCHHHHHH
GLADAMIDSLPLVVFTGQVATSVIGSDAFQEADILGITMPITKHSYQVRRPEDLPRVIKE
HHHHHHHHCCCEEEEECHHHHHHHCCCHHCCCCEEEEEECCCCCCEECCCCHHHHHHHHH
AFHIATTGRPGPVLIDIPKDVAAFEGEFRYDHEISLPGYQPVKEPNYLQIRKLVEAVSSA
HHHEEECCCCCCEEEECCCHHHHCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHC
KKPVILAGAGVLHGKASEDLKNYAEQQQIPVAHTLLGLGGFPADHPLFLGMAGMHGTYTA
CCCEEEECCCEECCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEEECCCCCCEEC
NMALYHCDLLISIGARFDDRVTGNLKHFAKSAKVAHIDIDPAEIGKIIETQIPVVGDSKI
CCCEEEHHHHEECCCCCCCCCCCCHHHHHHCCCEEEEECCHHHHHHHHHHCCCCCCCCHH
VLQELLKQNGKQGQTEEWKQQLSEWKEEYPLWYTDNREEGLKPQKLIEYIHQFTNGEAIV
HHHHHHHHCCCCCCHHHHHHHHHHHHHHCCEEEECCHHHCCCHHHHHHHHHHHCCCCEEE
ATDVGQHQMWAAQFYPFRKADKWVTSGGLGTMGFGLPAAIGAQLADRNATVVAILGDGGF
EECCCCHHHHHHHHCCCHHHCCEECCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCC
QMTLQELDVIRQLNLPVKVVILNNECLGMVRQWQEIFYEERYSESKFSAQPDFVKLSEAY
EEHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHC
GIKGVRISSEEEAEEELKKALSSKEPAVIDVRVAKSEKVFPMIAPGKGLHEMVGVKP
CCCEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEECCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]