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The map label for this gene is nadB [H]

Identifier: 154686922

GI number: 154686922

Start: 2612403

End: 2613968

Strand: Reverse

Name: nadB [H]

Synonym: RBAM_024920

Alternate gene names: 154686922

Gene position: 2613968-2612403 (Counterclockwise)

Preceding gene: 154686925

Following gene: 154686921

Centisome position: 66.71

GC content: 50.26

Gene sequence:

>1566_bases
ATGTCTGAAAAAACAATTATCGTCATTGGTTCGGGGACGGCGGCCCTTGCCTGTGCTTCCTCATTTCCTCCGTCATACCA
AGTGACCGTCATCACGAAGAGAAACGTAACGAACAGCAATTCGCATTATGCTCAAGGCGGGATTGCTGTTTCATACGCAA
AAGATGACTCGATAGAATCCCATGTGAACGATACACTGTATGCGGGGTGCGGACACAATGACGTGACAGCCGTGCGGCAT
TTGCTGAAAAGCGGACAGAGCATGATAAACAGGCTGATAGAAGGCCATTTCCCTTTTGATACGAAGTCGGACGGAACGTT
TTGTCTCGGCAGGGAAGGCGCTCATTCCAAAAGGAGGATTCTGCACGCCGGGGGAGATGCGACCGGACGGATGTTAGTTG
AGTTTCTGGTTCAGCAGCTGAGGGACGGCATACGGATCATTGAACACGAGACGGTTGTTGATCTGCTGATTGAACACGGA
CGATGCGCAGGTGTTGCCGTAAAGGATGCTTCAGGCGCTGTTTCTTACAGAAGAGCGGATTGTGTAGTGCTTGCATCAGG
CGGCTGCGGCAGTCTGTACCAATATCACACAAATGATTCCGCCATAACGGGAGACGGCATCGCTCTTGCCTTCCGCGCGG
GAGCTGAGATCACGGATATGGAGTTCACCCAGTTCCACCCGACCCTCCTTGTGAAAAACGGGACTGTGTACGGCCTGATT
TCTGAAGCAGTGAGAGGAGAAGGCGGATATTTAGCGGATCGCTTCGGGCGCAGGATCATGGCTGGCAGACACGCGCTTGA
AGATTTGGCGCCCCGGGATGTGGTTTCACGGGCGATTTATGAAGAAATGATGAAAGAAAATCCTGTTTTTATAAATTGCA
GCCGAATAGCCGATTTTAAGAAACGTTTTCCGACCATAACAGGTATTTGTCAAACAGCCGGTGTTGATCTTGAAAGCGGG
ATTCCAGTAGCACCCGGAATGCATTTTTTAATGGGCGGAGTAAAAGCCGATACATGGGGGAAAACGTCCGTGCCTGATTT
GTACGCCATAGGCGAGACCGCCTGCACAGGATTGCACGGGGCGAACCGCCTTGCGAGCAACTCACTGCTGGAAGCGCTGG
CAAGCGGGAAACGATGTGCTGAACATATCACTCAAACACCGTTAAACGGAAGATATAAAAAAACCCCGAAGAAATCTGAC
GTCATCTTACAAGTGCCGGACACAACGCTTCATGTGCTCCAAGCCAAAATGACAACCGGAGCGGGCATTGTCAGGGAAAA
AGAAAACATGCAGGCGCTGGCATCCTGGCTTAACAACGTATCTGTGCGTCATATAAATGTGAAGGAAATCACAATAGAAC
AAGCCGAGCTATCCTGCCTATGGAAGACTGCGCAACTTGTTGTGACCTCAGCCCTTATTCGGGAAGAAAGCAGGGGGGCT
CATTTTCGCGCTGATTTTCCCGGGACGGAGAAAGAGTGGCACGGAAAACAAATCATTCATTCAACATCTGGAACAGCTAT
CAGAAAAAACGAAAGGATTGGGGAACTATGGACATTCTGCAGCTGA

Upstream 100 bases:

>100_bases
CCGTCTCCTATCCGTTTATGTTAAAAAAAGCTTGAGTTTATTTTATCGTTGTGTAAATATAGGTGTCAAGACAGGTGTAA
ATATAACAGGAGGGTGATGT

Downstream 100 bases:

>100_bases
AACAAATGCTGACTCATTTTTTTCAGGAAGATATCGGAACGGGTGATGTCACGTCACAAGCGATTTTTTCTGATCAGCAA
TGTAAAGCGCGAATCATCAC

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MSEKTIIVIGSGTAALACASSFPPSYQVTVITKRNVTNSNSHYAQGGIAVSYAKDDSIESHVNDTLYAGCGHNDVTAVRH
LLKSGQSMINRLIEGHFPFDTKSDGTFCLGREGAHSKRRILHAGGDATGRMLVEFLVQQLRDGIRIIEHETVVDLLIEHG
RCAGVAVKDASGAVSYRRADCVVLASGGCGSLYQYHTNDSAITGDGIALAFRAGAEITDMEFTQFHPTLLVKNGTVYGLI
SEAVRGEGGYLADRFGRRIMAGRHALEDLAPRDVVSRAIYEEMMKENPVFINCSRIADFKKRFPTITGICQTAGVDLESG
IPVAPGMHFLMGGVKADTWGKTSVPDLYAIGETACTGLHGANRLASNSLLEALASGKRCAEHITQTPLNGRYKKTPKKSD
VILQVPDTTLHVLQAKMTTGAGIVREKENMQALASWLNNVSVRHINVKEITIEQAELSCLWKTAQLVVTSALIREESRGA
HFRADFPGTEKEWHGKQIIHSTSGTAIRKNERIGELWTFCS

Sequences:

>Translated_521_residues
MSEKTIIVIGSGTAALACASSFPPSYQVTVITKRNVTNSNSHYAQGGIAVSYAKDDSIESHVNDTLYAGCGHNDVTAVRH
LLKSGQSMINRLIEGHFPFDTKSDGTFCLGREGAHSKRRILHAGGDATGRMLVEFLVQQLRDGIRIIEHETVVDLLIEHG
RCAGVAVKDASGAVSYRRADCVVLASGGCGSLYQYHTNDSAITGDGIALAFRAGAEITDMEFTQFHPTLLVKNGTVYGLI
SEAVRGEGGYLADRFGRRIMAGRHALEDLAPRDVVSRAIYEEMMKENPVFINCSRIADFKKRFPTITGICQTAGVDLESG
IPVAPGMHFLMGGVKADTWGKTSVPDLYAIGETACTGLHGANRLASNSLLEALASGKRCAEHITQTPLNGRYKKTPKKSD
VILQVPDTTLHVLQAKMTTGAGIVREKENMQALASWLNNVSVRHINVKEITIEQAELSCLWKTAQLVVTSALIREESRGA
HFRADFPGTEKEWHGKQIIHSTSGTAIRKNERIGELWTFCS
>Mature_520_residues
SEKTIIVIGSGTAALACASSFPPSYQVTVITKRNVTNSNSHYAQGGIAVSYAKDDSIESHVNDTLYAGCGHNDVTAVRHL
LKSGQSMINRLIEGHFPFDTKSDGTFCLGREGAHSKRRILHAGGDATGRMLVEFLVQQLRDGIRIIEHETVVDLLIEHGR
CAGVAVKDASGAVSYRRADCVVLASGGCGSLYQYHTNDSAITGDGIALAFRAGAEITDMEFTQFHPTLLVKNGTVYGLIS
EAVRGEGGYLADRFGRRIMAGRHALEDLAPRDVVSRAIYEEMMKENPVFINCSRIADFKKRFPTITGICQTAGVDLESGI
PVAPGMHFLMGGVKADTWGKTSVPDLYAIGETACTGLHGANRLASNSLLEALASGKRCAEHITQTPLNGRYKKTPKKSDV
ILQVPDTTLHVLQAKMTTGAGIVREKENMQALASWLNNVSVRHINVKEITIEQAELSCLWKTAQLVVTSALIREESRGAH
FRADFPGTEKEWHGKQIIHSTSGTAIRKNERIGELWTFCS

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=520, Percent_Identity=30.5769230769231, Blast_Score=170, Evalue=3e-42,
Organism=Escherichia coli, GI1788928, Length=515, Percent_Identity=38.4466019417476, Blast_Score=298, Evalue=5e-82,
Organism=Escherichia coli, GI1790597, Length=519, Percent_Identity=30.8285163776493, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1786942, Length=525, Percent_Identity=32, Blast_Score=190, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17505833, Length=524, Percent_Identity=31.6793893129771, Blast_Score=183, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17550100, Length=523, Percent_Identity=32.1223709369025, Blast_Score=182, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI71986328, Length=435, Percent_Identity=25.5172413793103, Blast_Score=83, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6322701, Length=531, Percent_Identity=31.4500941619586, Blast_Score=174, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6322416, Length=526, Percent_Identity=30.6083650190114, Blast_Score=170, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6322511, Length=478, Percent_Identity=26.1506276150628, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6320788, Length=477, Percent_Identity=27.4633123689727, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI17137288, Length=527, Percent_Identity=31.1195445920304, Blast_Score=185, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24655642, Length=527, Percent_Identity=31.1195445920304, Blast_Score=185, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24655647, Length=527, Percent_Identity=31.1195445920304, Blast_Score=185, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24663005, Length=527, Percent_Identity=31.4990512333966, Blast_Score=179, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 56541; Mature: 56410

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKTIIVIGSGTAALACASSFPPSYQVTVITKRNVTNSNSHYAQGGIAVSYAKDDSIES
CCCCEEEEEECCHHHEEECCCCCCCEEEEEEEECCCCCCCCCEECCCEEEEECCCCCHHH
HVNDTLYAGCGHNDVTAVRHLLKSGQSMINRLIEGHFPFDTKSDGTFCLGREGAHSKRRI
HCCCCEEECCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCEE
LHAGGDATGRMLVEFLVQQLRDGIRIIEHETVVDLLIEHGRCAGVAVKDASGAVSYRRAD
EECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEECCC
CVVLASGGCGSLYQYHTNDSAITGDGIALAFRAGAEITDMEFTQFHPTLLVKNGTVYGLI
EEEEECCCCCCCEEEECCCCEEECCCEEEEEECCCCEECCCHHCCCCEEEEECCEEEEEE
SEAVRGEGGYLADRFGRRIMAGRHALEDLAPRDVVSRAIYEEMMKENPVFINCSRIADFK
HHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHH
KRFPTITGICQTAGVDLESGIPVAPGMHFLMGGVKADTWGKTSVPDLYAIGETACTGLHG
HHCCCHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEECCHHHHCCCCC
ANRLASNSLLEALASGKRCAEHITQTPLNGRYKKTPKKSDVILQVPDTTLHVLQAKMTTG
HHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCHHEEHHHHHHCCC
AGIVREKENMQALASWLNNVSVRHINVKEITIEQAELSCLWKTAQLVVTSALIREESRGA
CCCEECHHHHHHHHHHHCCCEEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCC
HFRADFPGTEKEWHGKQIIHSTSGTAIRKNERIGELWTFCS
EEEECCCCCCCCCCCCEEEECCCCCEEECCCCHHHHHHCCC
>Mature Secondary Structure 
SEKTIIVIGSGTAALACASSFPPSYQVTVITKRNVTNSNSHYAQGGIAVSYAKDDSIES
CCCEEEEEECCHHHEEECCCCCCCEEEEEEEECCCCCCCCCEECCCEEEEECCCCCHHH
HVNDTLYAGCGHNDVTAVRHLLKSGQSMINRLIEGHFPFDTKSDGTFCLGREGAHSKRRI
HCCCCEEECCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCEE
LHAGGDATGRMLVEFLVQQLRDGIRIIEHETVVDLLIEHGRCAGVAVKDASGAVSYRRAD
EECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEECCC
CVVLASGGCGSLYQYHTNDSAITGDGIALAFRAGAEITDMEFTQFHPTLLVKNGTVYGLI
EEEEECCCCCCCEEEECCCCEEECCCEEEEEECCCCEECCCHHCCCCEEEEECCEEEEEE
SEAVRGEGGYLADRFGRRIMAGRHALEDLAPRDVVSRAIYEEMMKENPVFINCSRIADFK
HHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHH
KRFPTITGICQTAGVDLESGIPVAPGMHFLMGGVKADTWGKTSVPDLYAIGETACTGLHG
HHCCCHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEECCHHHHCCCCC
ANRLASNSLLEALASGKRCAEHITQTPLNGRYKKTPKKSDVILQVPDTTLHVLQAKMTTG
HHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCHHEEHHHHHHCCC
AGIVREKENMQALASWLNNVSVRHINVKEITIEQAELSCLWKTAQLVVTSALIREESRGA
CCCEECHHHHHHHHHHHCCCEEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCC
HFRADFPGTEKEWHGKQIIHSTSGTAIRKNERIGELWTFCS
EEEECCCCCCCCCCCCEEEECCCCCEEECCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8444804; 9384377 [H]