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The map label for this gene is glyS [H]

Identifier: 154686786

GI number: 154686786

Start: 2475226

End: 2477265

Strand: Reverse

Name: glyS [H]

Synonym: RBAM_023560

Alternate gene names: 154686786

Gene position: 2477265-2475226 (Counterclockwise)

Preceding gene: 154686787

Following gene: 154686785

Centisome position: 63.22

GC content: 47.01

Gene sequence:

>2040_bases
ATGAGTAAACAAGACTTGCTTTTAGAGATCGGACTCGAGGAAATGCCGGCGCGGTTTTTACATGACAGCATGGTGCAGCT
CGGTGAAAAGCTGACCGGCTGGCTGGCTGAAAAAAATATCGCCCACGGCGAAGTGAAACTTTTTAATACGCCGAGACGTC
TGGCAGTATATATAAAAGACGTAGCAGAAAAACAGGCTGATATTCAGGAAGAAGCAAAAGGCCCTGCGAAAAAAATCGCT
CTGGATGCTGACGGAAACTGGACAAAAGCCGCTATCGGATTTTCAAAAGGCCAAGGCGCCGGAGTGGATGACCTGTATAT
AAAAGATGTAAACGGCACGGAATATGTCTTCGTTCAAAAATTCCAGGCGGGACAAGAGACGAAGACACTTTTACCGCAGC
TGGAAAATTTGATTGCCGGAATGCATTTTCCGAAAAACATGCGCTGGGGCACGCAGGATCTGCGCTATATCCGTCCGATC
AAATGGATTATCGCTCTCTTTGGAAAAGACGTCATTCCGTTTAAAATCACAAATGTAGAAACGGGCCGGAACACGCAGGG
ACACCGCTTTCTCGGCCAAGAAGTATCTGTTGATGCACCGTCATCATACGAAAAGCTGCTGAAAGAACAAAGCGTCATTG
CTGACCCGAAAGAACGCAAACAAATGATCCAAAGCCAGCTGGACGCGCTTGCAGCCGAGAAGAAATGGAACATTCCGATC
GATGAGGATCTGCTGAACGAAGTCAACCATCTGGTGGAATACCCGACGGCGCTTTACGGATCTTTTGAATCCGAGTTTCT
TTCTATCCCTGAAGAAGTGCTTGTGACAACAATGAAAGAGCATCAGCGCTACTTCCCGGTAAAAGATGAAAACGGGAATC
TGCTTCCTCATTTTATTACGGTAAGAAACGGGAACAGCCATGCAATTGAAAATGTTGCCCGCGGAAACGAGAAAGTGCTG
AGAGCAAGGCTTTCAGATGCTGCGTTCTTCTACAAAGAAGACCAAAAGCTGAACATTGATGAAAATGTGAAGAAACTGGA
AAATATCGTTTTCCATGAAGAGCTCGGCTCCCTTGGAGATAAAGTAAGAAGAGTGACCGCCATTGCGGGCAAACTTGCCG
TCCGCCTGAAAGCGGATGAACAGACGCAAAAACTTGTGAAGCGCGCCGCAGAGATTTCTAAATTTGACCTGGTTACACAT
ATGATCTATGAGTTCCCAGAGCTTCAAGGCGTGATGGGAGAAAAATATGCGCGCATGCTCGGTGAAGATGATGCCGTCGC
TGCCGCTGTCAATGAGCACTATATGCCGAGAGCGGCCGGGGGAGAAGTGCCTTCAACCTTTACCGGCGCGGTCGTGGCGT
TAGCTGATAAACTAGACACGATTGCTTCCTTCTTCAGCATTGACGTAATTCCGACAGGTTCTCAGGATCCGTACGGATTG
CGCCGTCAGGCAAGCGGCATCGTTTCCATTCTGATCGACCGCAATTGGGGCATTTCTTTTAAAGAGCTGCTGTCATTGAC
AGAAACGGAAAAAGAAAATGAGCTCCTCGATTTCTTTACACAGCGTCTGAAATACGTCTTAAACGCTGAAGACATCCGTC
ATGACGTTATTGAAGCCGTTCTGGACAGCACAGAGCTTGAGCCATATGCAGCCGTTCATAAGGCAAGAGTGCTTGAGAAG
CACCTCGGCACACCCGGCTTTAAAGAAACGGCTGAATCACTCGGCCGGGTCATCTCCATCAGCAAAAAAGGCGAGAGCCG
CGATATCAATCCGGATCTGTTTGAAAATGAACAGGAGAAAAAGCTGTTTGACGCTTATCAAAAAACAAAAGACAGTGTCG
CGGAAAGCTTTGCCTCCAAAAACTATGAACAGGCGCTTGCCGTGCTCAGCGGATTAAAGGAGCCGATTGAAGACTACTTT
AATCACACGATGGTAAATGCGGACGATGAAGCATTAAAAACCAACCGTTTAGCGCAAATGACCGCTCTTGCAGATGTGAT
CAAGTCTTTTGCTAATATGAATCATCTTATTGTAAAATAA

Upstream 100 bases:

>100_bases
ATCGGCCGTGTCCGCAACTTAGCGAGAAAAGTGGCAAAAACGTATTATGAAGAACGGGAAAAATTAGGGTTCCCAATGCT
TAAAGAGGAGGGCTCTTCCC

Downstream 100 bases:

>100_bases
AGAAAAAGACGGTTGCTGTGTGATCGGCAATCGTCTTTTCCCCTTTTACATAAACATCACAGAATGAAAACATAGATAAA
GAAACCCTTCCATTAAGGTG

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 679; Mature: 678

Protein sequence:

>679_residues
MSKQDLLLEIGLEEMPARFLHDSMVQLGEKLTGWLAEKNIAHGEVKLFNTPRRLAVYIKDVAEKQADIQEEAKGPAKKIA
LDADGNWTKAAIGFSKGQGAGVDDLYIKDVNGTEYVFVQKFQAGQETKTLLPQLENLIAGMHFPKNMRWGTQDLRYIRPI
KWIIALFGKDVIPFKITNVETGRNTQGHRFLGQEVSVDAPSSYEKLLKEQSVIADPKERKQMIQSQLDALAAEKKWNIPI
DEDLLNEVNHLVEYPTALYGSFESEFLSIPEEVLVTTMKEHQRYFPVKDENGNLLPHFITVRNGNSHAIENVARGNEKVL
RARLSDAAFFYKEDQKLNIDENVKKLENIVFHEELGSLGDKVRRVTAIAGKLAVRLKADEQTQKLVKRAAEISKFDLVTH
MIYEFPELQGVMGEKYARMLGEDDAVAAAVNEHYMPRAAGGEVPSTFTGAVVALADKLDTIASFFSIDVIPTGSQDPYGL
RRQASGIVSILIDRNWGISFKELLSLTETEKENELLDFFTQRLKYVLNAEDIRHDVIEAVLDSTELEPYAAVHKARVLEK
HLGTPGFKETAESLGRVISISKKGESRDINPDLFENEQEKKLFDAYQKTKDSVAESFASKNYEQALAVLSGLKEPIEDYF
NHTMVNADDEALKTNRLAQMTALADVIKSFANMNHLIVK

Sequences:

>Translated_679_residues
MSKQDLLLEIGLEEMPARFLHDSMVQLGEKLTGWLAEKNIAHGEVKLFNTPRRLAVYIKDVAEKQADIQEEAKGPAKKIA
LDADGNWTKAAIGFSKGQGAGVDDLYIKDVNGTEYVFVQKFQAGQETKTLLPQLENLIAGMHFPKNMRWGTQDLRYIRPI
KWIIALFGKDVIPFKITNVETGRNTQGHRFLGQEVSVDAPSSYEKLLKEQSVIADPKERKQMIQSQLDALAAEKKWNIPI
DEDLLNEVNHLVEYPTALYGSFESEFLSIPEEVLVTTMKEHQRYFPVKDENGNLLPHFITVRNGNSHAIENVARGNEKVL
RARLSDAAFFYKEDQKLNIDENVKKLENIVFHEELGSLGDKVRRVTAIAGKLAVRLKADEQTQKLVKRAAEISKFDLVTH
MIYEFPELQGVMGEKYARMLGEDDAVAAAVNEHYMPRAAGGEVPSTFTGAVVALADKLDTIASFFSIDVIPTGSQDPYGL
RRQASGIVSILIDRNWGISFKELLSLTETEKENELLDFFTQRLKYVLNAEDIRHDVIEAVLDSTELEPYAAVHKARVLEK
HLGTPGFKETAESLGRVISISKKGESRDINPDLFENEQEKKLFDAYQKTKDSVAESFASKNYEQALAVLSGLKEPIEDYF
NHTMVNADDEALKTNRLAQMTALADVIKSFANMNHLIVK
>Mature_678_residues
SKQDLLLEIGLEEMPARFLHDSMVQLGEKLTGWLAEKNIAHGEVKLFNTPRRLAVYIKDVAEKQADIQEEAKGPAKKIAL
DADGNWTKAAIGFSKGQGAGVDDLYIKDVNGTEYVFVQKFQAGQETKTLLPQLENLIAGMHFPKNMRWGTQDLRYIRPIK
WIIALFGKDVIPFKITNVETGRNTQGHRFLGQEVSVDAPSSYEKLLKEQSVIADPKERKQMIQSQLDALAAEKKWNIPID
EDLLNEVNHLVEYPTALYGSFESEFLSIPEEVLVTTMKEHQRYFPVKDENGNLLPHFITVRNGNSHAIENVARGNEKVLR
ARLSDAAFFYKEDQKLNIDENVKKLENIVFHEELGSLGDKVRRVTAIAGKLAVRLKADEQTQKLVKRAAEISKFDLVTHM
IYEFPELQGVMGEKYARMLGEDDAVAAAVNEHYMPRAAGGEVPSTFTGAVVALADKLDTIASFFSIDVIPTGSQDPYGLR
RQASGIVSILIDRNWGISFKELLSLTETEKENELLDFFTQRLKYVLNAEDIRHDVIEAVLDSTELEPYAAVHKARVLEKH
LGTPGFKETAESLGRVISISKKGESRDINPDLFENEQEKKLFDAYQKTKDSVAESFASKNYEQALAVLSGLKEPIEDYFN
HTMVNADDEALKTNRLAQMTALADVIKSFANMNHLIVK

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=694, Percent_Identity=35.3025936599424, Blast_Score=422, Evalue=1e-119,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 76358; Mature: 76227

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQDLLLEIGLEEMPARFLHDSMVQLGEKLTGWLAEKNIAHGEVKLFNTPRRLAVYIKD
CCCCCEEHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHH
VAEKQADIQEEAKGPAKKIALDADGNWTKAAIGFSKGQGAGVDDLYIKDVNGTEYVFVQK
HHHHHHHHHHHHCCCHHEEEECCCCCEEEEEEECCCCCCCCCCCEEEEECCCCEEEEEEE
FQAGQETKTLLPQLENLIAGMHFPKNMRWGTQDLRYIRPIKWIIALFGKDVIPFKITNVE
HHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
TGRNTQGHRFLGQEVSVDAPSSYEKLLKEQSVIADPKERKQMIQSQLDALAAEKKWNIPI
CCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCC
DEDLLNEVNHLVEYPTALYGSFESEFLSIPEEVLVTTMKEHQRYFPVKDENGNLLPHFIT
CHHHHHHHHHHHHCCHHHHCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEE
VRNGNSHAIENVARGNEKVLRARLSDAAFFYKEDQKLNIDENVKKLENIVFHEELGSLGD
EECCCHHHHHHHHCCHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
KVRRVTAIAGKLAVRLKADEQTQKLVKRAAEISKFDLVTHMIYEFPELQGVMGEKYARML
HHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GEDDAVAAAVNEHYMPRAAGGEVPSTFTGAVVALADKLDTIASFFSIDVIPTGSQDPYGL
CCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCH
RRQASGIVSILIDRNWGISFKELLSLTETEKENELLDFFTQRLKYVLNAEDIRHDVIEAV
HHHHCCEEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
LDSTELEPYAAVHKARVLEKHLGTPGFKETAESLGRVISISKKGESRDINPDLFENEQEK
HCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEEECCCCCCCCCCHHHCCHHHH
KLFDAYQKTKDSVAESFASKNYEQALAVLSGLKEPIEDYFNHTMVNADDEALKTNRLAQM
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
TALADVIKSFANMNHLIVK
HHHHHHHHHHHCCCCEECC
>Mature Secondary Structure 
SKQDLLLEIGLEEMPARFLHDSMVQLGEKLTGWLAEKNIAHGEVKLFNTPRRLAVYIKD
CCCCEEHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHH
VAEKQADIQEEAKGPAKKIALDADGNWTKAAIGFSKGQGAGVDDLYIKDVNGTEYVFVQK
HHHHHHHHHHHHCCCHHEEEECCCCCEEEEEEECCCCCCCCCCCEEEEECCCCEEEEEEE
FQAGQETKTLLPQLENLIAGMHFPKNMRWGTQDLRYIRPIKWIIALFGKDVIPFKITNVE
HHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
TGRNTQGHRFLGQEVSVDAPSSYEKLLKEQSVIADPKERKQMIQSQLDALAAEKKWNIPI
CCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCC
DEDLLNEVNHLVEYPTALYGSFESEFLSIPEEVLVTTMKEHQRYFPVKDENGNLLPHFIT
CHHHHHHHHHHHHCCHHHHCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEE
VRNGNSHAIENVARGNEKVLRARLSDAAFFYKEDQKLNIDENVKKLENIVFHEELGSLGD
EECCCHHHHHHHHCCHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
KVRRVTAIAGKLAVRLKADEQTQKLVKRAAEISKFDLVTHMIYEFPELQGVMGEKYARML
HHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GEDDAVAAAVNEHYMPRAAGGEVPSTFTGAVVALADKLDTIASFFSIDVIPTGSQDPYGL
CCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCH
RRQASGIVSILIDRNWGISFKELLSLTETEKENELLDFFTQRLKYVLNAEDIRHDVIEAV
HHHHCCEEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
LDSTELEPYAAVHKARVLEKHLGTPGFKETAESLGRVISISKKGESRDINPDLFENEQEK
HCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEEECCCCCCCCCCHHHCCHHHH
KLFDAYQKTKDSVAESFASKNYEQALAVLSGLKEPIEDYFNHTMVNADDEALKTNRLAQM
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
TALADVIKSFANMNHLIVK
HHHHHHHHHHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]