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The map label for this gene is buk [H]

Identifier: 154686666

GI number: 154686666

Start: 2371945

End: 2373054

Strand: Reverse

Name: buk [H]

Synonym: RBAM_022350

Alternate gene names: 154686666

Gene position: 2373054-2371945 (Counterclockwise)

Preceding gene: 154686667

Following gene: 154686665

Centisome position: 60.56

GC content: 49.73

Gene sequence:

>1110_bases
ATGTTCGAAAAGGAAAAACGCATTCTCATTCTGAATCCAGGCTCAACATCAACGAAAATCGGTGTCTTTCACAATGAACG
CTGTATCTTTGAAAAGACGCTGCGGCATCCCGAAGAAGAGCTAAAAACATTTAACAGCATCATCGACCAATACGAATTTC
GAAAAATAAGCATTCTTGAGTCTTTGCACGAACAGGGCATCAACATTTCAAAGTTCGATGCCGTTTGTGCCAGGGGCGGC
CTGCTCAGACCGATTGAGGGCGGCACATATGAAGTGAATGACGCCATGATCACTGATTTGAAAAACGGCTACAACGGCCA
GCATGCTTCTAATCTGGGCGGAATTATTGCCAGGGAAATAGCTGACGGATTAAATATCCCCGCGTATATCGTCGATCCGG
TCGTGGTGGATGAGATGACTCCGCTCGCTAAAGTATCAGGAATGCCTGAAATCAAGCGGAAAAGCATTTTCCATGCTTTG
AACCAAAAAGCTGTTGCCCGTCAGGCGGCGGCGTCCCTCGGCAAACGGTATGAACAGATGAAGATGATCGTCACTCATAT
GGGCGGCGGCACCACAGTCGGTGTCCATGATTGCGGACGGGTGACGGATGTTAACAACGGGCTTCACGGAGAAGGACCCT
TCAGCCCGGAGCGCGCGGGAACTGTTCCGGCGGGTGATTTGGTAGAAATGTGTTTTTCCGGCAAGTATTCAAAAGCCGAT
ATGATGAAAAAACTTGTCGGCACGGGCGGACTCTCCGGCTACCTGGGGACAACGGACGCGGTGAAGGTGGAAAAACGCAT
TCAGGCCGGAGATGAAAACGCACGTCTCATTTATGACGCCATGGCTTATCAAGTAGCAAAAGAAATCGGGGCGGCAAGCG
CCGTTTTAAAAGGAAAAGTAGACATTATCGTCCTGACGGGCGGATTGGCGTACGGGAAAAGTTTCGTATCCGCCATCAGA
TCGTACATAGATTGGATTTCGGATGTACTGGTGTACCCGGGAGAAAATGAACTGCAATCTCTCGCGCAAGGTGCGCTTCG
GGTTTTAGCCGGTGAGGAGCAGTCAAAGCAATATCAGATCGGCGAAGGAGTGAAAACAAATGGCGACTGA

Upstream 100 bases:

>100_bases
AGGAACGAATTGAGCGCATGCGCCGTTCAAGAAGCCAGTTTCTGCAAAACGGCCAAAGTATATTAAGCAGACGATAGGAA
TTTGATCTGGAGGTATGAAA

Downstream 100 bases:

>100_bases
GTATGATGTGGTCATTCTGGGCGGCGGCACGGGCGGTTATGTAGCCGCCATCAGAGCGGCCCAGCTCGGCTTAAAAACGG
CCGTTGTAGAAAAAGAAAAG

Product: butyrate kinase

Products: NA

Alternate protein names: BK; Branched-chain carboxylic acid kinase [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MFEKEKRILILNPGSTSTKIGVFHNERCIFEKTLRHPEEELKTFNSIIDQYEFRKISILESLHEQGINISKFDAVCARGG
LLRPIEGGTYEVNDAMITDLKNGYNGQHASNLGGIIAREIADGLNIPAYIVDPVVVDEMTPLAKVSGMPEIKRKSIFHAL
NQKAVARQAAASLGKRYEQMKMIVTHMGGGTTVGVHDCGRVTDVNNGLHGEGPFSPERAGTVPAGDLVEMCFSGKYSKAD
MMKKLVGTGGLSGYLGTTDAVKVEKRIQAGDENARLIYDAMAYQVAKEIGAASAVLKGKVDIIVLTGGLAYGKSFVSAIR
SYIDWISDVLVYPGENELQSLAQGALRVLAGEEQSKQYQIGEGVKTNGD

Sequences:

>Translated_369_residues
MFEKEKRILILNPGSTSTKIGVFHNERCIFEKTLRHPEEELKTFNSIIDQYEFRKISILESLHEQGINISKFDAVCARGG
LLRPIEGGTYEVNDAMITDLKNGYNGQHASNLGGIIAREIADGLNIPAYIVDPVVVDEMTPLAKVSGMPEIKRKSIFHAL
NQKAVARQAAASLGKRYEQMKMIVTHMGGGTTVGVHDCGRVTDVNNGLHGEGPFSPERAGTVPAGDLVEMCFSGKYSKAD
MMKKLVGTGGLSGYLGTTDAVKVEKRIQAGDENARLIYDAMAYQVAKEIGAASAVLKGKVDIIVLTGGLAYGKSFVSAIR
SYIDWISDVLVYPGENELQSLAQGALRVLAGEEQSKQYQIGEGVKTNGD
>Mature_369_residues
MFEKEKRILILNPGSTSTKIGVFHNERCIFEKTLRHPEEELKTFNSIIDQYEFRKISILESLHEQGINISKFDAVCARGG
LLRPIEGGTYEVNDAMITDLKNGYNGQHASNLGGIIAREIADGLNIPAYIVDPVVVDEMTPLAKVSGMPEIKRKSIFHAL
NQKAVARQAAASLGKRYEQMKMIVTHMGGGTTVGVHDCGRVTDVNNGLHGEGPFSPERAGTVPAGDLVEMCFSGKYSKAD
MMKKLVGTGGLSGYLGTTDAVKVEKRIQAGDENARLIYDAMAYQVAKEIGAASAVLKGKVDIIVLTGGLAYGKSFVSAIR
SYIDWISDVLVYPGENELQSLAQGALRVLAGEEQSKQYQIGEGVKTNGD

Specific function: Anaerobic degradation of L-threonine to propionate. [C]

COG id: COG3426

COG function: function code C; Butyrate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI145693184, Length=341, Percent_Identity=24.3401759530792, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR011245 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.7 [H]

Molecular weight: Translated: 39958; Mature: 39958

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEKEKRILILNPGSTSTKIGVFHNERCIFEKTLRHPEEELKTFNSIIDQYEFRKISILE
CCCCCCEEEEECCCCCCCEEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
SLHEQGINISKFDAVCARGGLLRPIEGGTYEVNDAMITDLKNGYNGQHASNLGGIIAREI
HHHHCCCCCHHHHHHHHCCCCCCCCCCCCEEECHHHHHHHHCCCCCCCHHHHHHHHHHHH
ADGLNIPAYIVDPVVVDEMTPLAKVSGMPEIKRKSIFHALNQKAVARQAAASLGKRYEQM
HCCCCCCHHEECCHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMIVTHMGGGTTVGVHDCGRVTDVNNGLHGEGPFSPERAGTVPAGDLVEMCFSGKYSKAD
HHHHHCCCCCCEECCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHH
MMKKLVGTGGLSGYLGTTDAVKVEKRIQAGDENARLIYDAMAYQVAKEIGAASAVLKGKV
HHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCE
DIIVLTGGLAYGKSFVSAIRSYIDWISDVLVYPGENELQSLAQGALRVLAGEEQSKQYQI
EEEEEECCHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHCCCCCCCCEEC
GEGVKTNGD
CCCCCCCCC
>Mature Secondary Structure
MFEKEKRILILNPGSTSTKIGVFHNERCIFEKTLRHPEEELKTFNSIIDQYEFRKISILE
CCCCCCEEEEECCCCCCCEEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
SLHEQGINISKFDAVCARGGLLRPIEGGTYEVNDAMITDLKNGYNGQHASNLGGIIAREI
HHHHCCCCCHHHHHHHHCCCCCCCCCCCCEEECHHHHHHHHCCCCCCCHHHHHHHHHHHH
ADGLNIPAYIVDPVVVDEMTPLAKVSGMPEIKRKSIFHALNQKAVARQAAASLGKRYEQM
HCCCCCCHHEECCHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMIVTHMGGGTTVGVHDCGRVTDVNNGLHGEGPFSPERAGTVPAGDLVEMCFSGKYSKAD
HHHHHCCCCCCEECCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHH
MMKKLVGTGGLSGYLGTTDAVKVEKRIQAGDENARLIYDAMAYQVAKEIGAASAVLKGKV
HHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCE
DIIVLTGGLAYGKSFVSAIRSYIDWISDVLVYPGENELQSLAQGALRVLAGEEQSKQYQI
EEEEEECCHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHCCCCCCCCEEC
GEGVKTNGD
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]