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The map label for this gene is dfnB [H]

Identifier: 154686636

GI number: 154686636

Start: 2341329

End: 2342660

Strand: Reverse

Name: dfnB [H]

Synonym: RBAM_022050

Alternate gene names: 154686636

Gene position: 2342660-2341329 (Counterclockwise)

Preceding gene: 154686637

Following gene: 154686635

Centisome position: 59.78

GC content: 47.22

Gene sequence:

>1332_bases
GTGACTGATAAGCTTAGGGAAACGCAAGACAGTGAGTTTCTCATTTTTAAAGGGCAGACTTTTACGTATGATGATTTGCT
GGCACGAATTAGCTTTTTTCAGAAAGAACTTGAACAAAACGGAATACGTGAAGGAGAATGTGTGGCGCTTATCGGGGATT
ATTCCCCGAACGCCATTTTTTTGCTGATGGCGCTTTTGTTAAACCGAAATATCGTTGTCCCTCTCAGCAATGAATCAAGG
GAAAAGCATCTCGATATGTTTGAAGACGCGAAAGTGAATCAAATCATTGAAATAAATGAAAATGACAGCTGGACGCTTCG
TGGAGGTTCTGAAAGCGCCTCTCACCCTCTGCTTGATGACCTGCGCGAGAGAGGGGAGTCAGGCATTATTATTTTCACAT
CGGGAACTTCCGGCAAAAGCAAAGCATCCGTGTTAAGCGCCGGCCGGCTGCTTGAAAAATATAAAACGGCGAAGAGAAAA
CCGCTGCGTACGCTCATTTTTCTGAAATTGGATCATATCGGCGGCATTAATACGCTGTTTGCGATTCTGTTTAACGGGGG
AACCATCGTCACATCAGACAGCAGAACGCCGGAGTCCGTTTATCAGGCGGTCGACAAGCATGCTGTCCAAGTGCTCCCCG
CCACCCCGACCTTTTTAAACATGCTGCTGATGTCCAAGGCGGATGCCGGCTATAACCTTTCATCACTCAAGCTGATCACT
TACGGCACAGAACCGATGCCCCAATCAACGCTGCGCGGCATTCACCGGCTGTTTCCGGATGTACGGCTGAAACAAACATA
CGGATTAACTGAACTGGGTATATTCTCAACGAAATCAAAAGACAGCCAATCCACTTGGATGAAAGTGGGCGGTGCCGGAA
CAGAGACCAAAATTACCGGAGGCACGCTTTGGGTCCGTTCTGAGTCCGCGATGCTCGGCTATTTAAACGCACCGTCACCT
TTCGATGAAGACGGCTGGTATGACACCGGTGACCAGGTGGAAACAGACGGCGAATATATAAGAATCCTTGGAAGAAAGAG
TGAAATCATTAATGTGGGCGGAGAGAAAGTGTTTCCCGCAGAGGTGGAAAGCGTCTTTCTGGAGATACCGAATGTCCGGG
ATGTTCTCATAACGGGCAGAAAAAATCCGATTACGGGCGAGATTGTTGCGGCGGAGGCCGTGCTTGAAAAAGAGGAAGAT
ATCAAATCTTTCAGAAAGCGGGCCGTAGCTCATTGCAAACAAAAGCTGCAGCCATATCAGATACCGCAGATCATCTCTGT
TACAGATCGGAAACTGTCTAATGACCGATTTAAAAAAATAAGAAACGTGTAA

Upstream 100 bases:

>100_bases
TTTCCAGCATTACAACCGTCGGCAGCATTTGTGATATTTATCAAAAATATATGGACAGTGCTCAATCATGAAAACAGAAA
GCGGGGGAGATATCAGCTGG

Downstream 100 bases:

>100_bases
AACAGGAGGCAAATATGAATAACGGAAAAGTAGCGGTCGTCAGCGGAGGAAGCCGGGGCCTGGGAAAAGCGATTGTACAG
ACGCTTTTGGATGAGGATTA

Product: DfnB

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MTDKLRETQDSEFLIFKGQTFTYDDLLARISFFQKELEQNGIREGECVALIGDYSPNAIFLLMALLLNRNIVVPLSNESR
EKHLDMFEDAKVNQIIEINENDSWTLRGGSESASHPLLDDLRERGESGIIIFTSGTSGKSKASVLSAGRLLEKYKTAKRK
PLRTLIFLKLDHIGGINTLFAILFNGGTIVTSDSRTPESVYQAVDKHAVQVLPATPTFLNMLLMSKADAGYNLSSLKLIT
YGTEPMPQSTLRGIHRLFPDVRLKQTYGLTELGIFSTKSKDSQSTWMKVGGAGTETKITGGTLWVRSESAMLGYLNAPSP
FDEDGWYDTGDQVETDGEYIRILGRKSEIINVGGEKVFPAEVESVFLEIPNVRDVLITGRKNPITGEIVAAEAVLEKEED
IKSFRKRAVAHCKQKLQPYQIPQIISVTDRKLSNDRFKKIRNV

Sequences:

>Translated_443_residues
MTDKLRETQDSEFLIFKGQTFTYDDLLARISFFQKELEQNGIREGECVALIGDYSPNAIFLLMALLLNRNIVVPLSNESR
EKHLDMFEDAKVNQIIEINENDSWTLRGGSESASHPLLDDLRERGESGIIIFTSGTSGKSKASVLSAGRLLEKYKTAKRK
PLRTLIFLKLDHIGGINTLFAILFNGGTIVTSDSRTPESVYQAVDKHAVQVLPATPTFLNMLLMSKADAGYNLSSLKLIT
YGTEPMPQSTLRGIHRLFPDVRLKQTYGLTELGIFSTKSKDSQSTWMKVGGAGTETKITGGTLWVRSESAMLGYLNAPSP
FDEDGWYDTGDQVETDGEYIRILGRKSEIINVGGEKVFPAEVESVFLEIPNVRDVLITGRKNPITGEIVAAEAVLEKEED
IKSFRKRAVAHCKQKLQPYQIPQIISVTDRKLSNDRFKKIRNV
>Mature_442_residues
TDKLRETQDSEFLIFKGQTFTYDDLLARISFFQKELEQNGIREGECVALIGDYSPNAIFLLMALLLNRNIVVPLSNESRE
KHLDMFEDAKVNQIIEINENDSWTLRGGSESASHPLLDDLRERGESGIIIFTSGTSGKSKASVLSAGRLLEKYKTAKRKP
LRTLIFLKLDHIGGINTLFAILFNGGTIVTSDSRTPESVYQAVDKHAVQVLPATPTFLNMLLMSKADAGYNLSSLKLITY
GTEPMPQSTLRGIHRLFPDVRLKQTYGLTELGIFSTKSKDSQSTWMKVGGAGTETKITGGTLWVRSESAMLGYLNAPSPF
DEDGWYDTGDQVETDGEYIRILGRKSEIINVGGEKVFPAEVESVFLEIPNVRDVLITGRKNPITGEIVAAEAVLEKEEDI
KSFRKRAVAHCKQKLQPYQIPQIISVTDRKLSNDRFKKIRNV

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=344, Percent_Identity=28.4883720930233, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI115511026, Length=103, Percent_Identity=38.8349514563107, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1788107, Length=326, Percent_Identity=27.6073619631902, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI145693145, Length=333, Percent_Identity=26.4264264264264, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1786810, Length=272, Percent_Identity=23.8970588235294, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788595, Length=449, Percent_Identity=23.6080178173719, Blast_Score=72, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17560140, Length=453, Percent_Identity=23.3995584988962, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI71996755, Length=446, Percent_Identity=26.6816143497758, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32563687, Length=473, Percent_Identity=25.5813953488372, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17531443, Length=339, Percent_Identity=29.4985250737463, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17558820, Length=335, Percent_Identity=27.4626865671642, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17559526, Length=327, Percent_Identity=25.9938837920489, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71994694, Length=378, Percent_Identity=26.4550264550265, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71994703, Length=378, Percent_Identity=26.4550264550265, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71994690, Length=378, Percent_Identity=26.4550264550265, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI25141359, Length=420, Percent_Identity=24.5238095238095, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17560308, Length=324, Percent_Identity=25.6172839506173, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71985884, Length=296, Percent_Identity=24.6621621621622, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17557194, Length=297, Percent_Identity=25.2525252525253, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17538037, Length=297, Percent_Identity=25.5892255892256, Blast_Score=72, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17550940, Length=425, Percent_Identity=23.7647058823529, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=331, Percent_Identity=25.0755287009063, Blast_Score=83, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6319264, Length=137, Percent_Identity=30.6569343065693, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24648260, Length=433, Percent_Identity=24.0184757505774, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24581924, Length=345, Percent_Identity=27.8260869565217, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21356947, Length=314, Percent_Identity=25.4777070063694, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24653035, Length=346, Percent_Identity=26.0115606936416, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI18859661, Length=319, Percent_Identity=26.0188087774295, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI161076582, Length=284, Percent_Identity=26.7605633802817, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648257, Length=306, Percent_Identity=26.797385620915, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI281365686, Length=312, Percent_Identity=23.3974358974359, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI21356441, Length=288, Percent_Identity=25, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21358303, Length=315, Percent_Identity=24.7619047619048, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 49461; Mature: 49330

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKLRETQDSEFLIFKGQTFTYDDLLARISFFQKELEQNGIREGECVALIGDYSPNAIF
CCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHH
LLMALLLNRNIVVPLSNESREKHLDMFEDAKVNQIIEINENDSWTLRGGSESASHPLLDD
HHHHHHHCCCEEEECCCCCHHHHCCHHHCCCCCEEEEECCCCCEEEECCCCCCCCCHHHH
LRERGESGIIIFTSGTSGKSKASVLSAGRLLEKYKTAKRKPLRTLIFLKLDHIGGINTLF
HHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHH
AILFNGGTIVTSDSRTPESVYQAVDKHAVQVLPATPTFLNMLLMSKADAGYNLSSLKLIT
HHHCCCCEEEECCCCCHHHHHHHHHHHHEEEECCCHHHHHHHHHHCCCCCCCCCEEEEEE
YGTEPMPQSTLRGIHRLFPDVRLKQTYGLTELGIFSTKSKDSQSTWMKVGGAGTETKITG
ECCCCCCHHHHHHHHHHCCCCEEHHHCCCCCCCEEECCCCCCCCHHEEECCCCCCEEEEC
GTLWVRSESAMLGYLNAPSPFDEDGWYDTGDQVETDGEYIRILGRKSEIINVGGEKVFPA
CEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEECCCCEECHH
EVESVFLEIPNVRDVLITGRKNPITGEIVAAEAVLEKEEDIKSFRKRAVAHCKQKLQPYQ
HHHHHHHCCCCCCEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IPQIISVTDRKLSNDRFKKIRNV
CCHHHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure 
TDKLRETQDSEFLIFKGQTFTYDDLLARISFFQKELEQNGIREGECVALIGDYSPNAIF
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHH
LLMALLLNRNIVVPLSNESREKHLDMFEDAKVNQIIEINENDSWTLRGGSESASHPLLDD
HHHHHHHCCCEEEECCCCCHHHHCCHHHCCCCCEEEEECCCCCEEEECCCCCCCCCHHHH
LRERGESGIIIFTSGTSGKSKASVLSAGRLLEKYKTAKRKPLRTLIFLKLDHIGGINTLF
HHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHH
AILFNGGTIVTSDSRTPESVYQAVDKHAVQVLPATPTFLNMLLMSKADAGYNLSSLKLIT
HHHCCCCEEEECCCCCHHHHHHHHHHHHEEEECCCHHHHHHHHHHCCCCCCCCCEEEEEE
YGTEPMPQSTLRGIHRLFPDVRLKQTYGLTELGIFSTKSKDSQSTWMKVGGAGTETKITG
ECCCCCCHHHHHHHHHHCCCCEEHHHCCCCCCCEEECCCCCCCCHHEEECCCCCCEEEEC
GTLWVRSESAMLGYLNAPSPFDEDGWYDTGDQVETDGEYIRILGRKSEIINVGGEKVFPA
CEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEECCCCEECHH
EVESVFLEIPNVRDVLITGRKNPITGEIVAAEAVLEKEEDIKSFRKRAVAHCKQKLQPYQ
HHHHHHHCCCCCCEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IPQIISVTDRKLSNDRFKKIRNV
CCHHHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1629163; 8566759; 9387221; 9384377 [H]