BacMap

Definition	Bacillus amyloliquefaciens FZB42, complete genome.
Accession	NC_009725
Length	3,918,589

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The map label for this gene is ppiB [H]

Identifier: 154686577

GI number: 154686577

Start: 2238335

End: 2238766

Strand: Reverse

Name: ppiB [H]

Synonym: RBAM_021460

Alternate gene names: 154686577

Gene position: 2238766-2238335 (Counterclockwise)

Preceding gene: 154686579

Following gene: 154686576

Centisome position: 57.13

GC content: 46.3

Gene sequence:

>432_bases
ATGAAAACTGGTTACATCGAAATGGAAAACGGCAGCAGAATTGAATTTGAACTTTATCCGAATGAAGCGCCTAATACAGT
CGCTAACTTTGAAAACCTGGCGAATACGGGCTTTTATGACGGCGTTATTTTCCACCGCGTGATTCCGGGCTTTGTCAGCC
AAGGCGGAGATCCGACTGGGACAGGCATGGGCGGAAGCGGAAAAACGATCAAATGTGAAACAGAAGGCAATCCGCATAAG
CACGAAGCCGGTTCTTTATCAATGGCGCATGCCGGTAAAGACACGGGTTCAAGCCAATTCTTCATCGTACATGAGCCGCA
GCCGCATTTAAACGGCGTTCATACTGTATTCGGAAAAGTGACAAGCGGTTTAGAGCATGCGAAAAACATGTCTAACGGAG
ACAAAATGAAAGAAATCCGTGTAGAAGGATAA

Upstream 100 bases:

>100_bases
CCTCTTCCTTCAAAAGAATATCAAAAAGAAAGCCGGCTTACAATTGAAATTCATATTGAAGAAGGCTTTGACAAAAAACA
ATATGGAAGTAGGAATGAGA

Downstream 100 bases:

>100_bases
TGACGGAAAACCCGCCTTTTTCAGGCGGGTTTTTTTATGTATATGAAAATTCTCATGTGTAAATCATGAAAAAATACATA
TATGAACGATAGCGGTTCCT

Product: PpiB

Products: NA

Alternate protein names: PPIase B; Rotamase B [H]

Number of amino acids: Translated: 143; Mature: 143

Protein sequence:

>143_residues
MKTGYIEMENGSRIEFELYPNEAPNTVANFENLANTGFYDGVIFHRVIPGFVSQGGDPTGTGMGGSGKTIKCETEGNPHK
HEAGSLSMAHAGKDTGSSQFFIVHEPQPHLNGVHTVFGKVTSGLEHAKNMSNGDKMKEIRVEG

Sequences:

>Translated_143_residues
MKTGYIEMENGSRIEFELYPNEAPNTVANFENLANTGFYDGVIFHRVIPGFVSQGGDPTGTGMGGSGKTIKCETEGNPHK
HEAGSLSMAHAGKDTGSSQFFIVHEPQPHLNGVHTVFGKVTSGLEHAKNMSNGDKMKEIRVEG
>Mature_143_residues
MKTGYIEMENGSRIEFELYPNEAPNTVANFENLANTGFYDGVIFHRVIPGFVSQGGDPTGTGMGGSGKTIKCETEGNPHK
HEAGSLSMAHAGKDTGSSQFFIVHEPQPHLNGVHTVFGKVTSGLEHAKNMSNGDKMKEIRVEG

Specific function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides [H]

COG id: COG0652

COG function: function code O; Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase cyclophilin-type domain [H]

Homologues:

Organism=Homo sapiens, GI7706339, Length=123, Percent_Identity=47.1544715447154, Blast_Score=105, Evalue=1e-23,
Organism=Homo sapiens, GI24308049, Length=122, Percent_Identity=46.7213114754098, Blast_Score=104, Evalue=2e-23,
Organism=Homo sapiens, GI19557636, Length=115, Percent_Identity=46.0869565217391, Blast_Score=97, Evalue=6e-21,
Organism=Homo sapiens, GI64276486, Length=111, Percent_Identity=45.945945945946, Blast_Score=94, Evalue=4e-20,
Organism=Homo sapiens, GI10863927, Length=144, Percent_Identity=43.75, Blast_Score=89, Evalue=2e-18,
Organism=Homo sapiens, GI4826932, Length=139, Percent_Identity=40.2877697841727, Blast_Score=82, Evalue=1e-16,
Organism=Homo sapiens, GI42560244, Length=135, Percent_Identity=41.4814814814815, Blast_Score=82, Evalue=1e-16,
Organism=Homo sapiens, GI4758950, Length=131, Percent_Identity=44.2748091603053, Blast_Score=81, Evalue=3e-16,
Organism=Homo sapiens, GI256017253, Length=118, Percent_Identity=44.9152542372881, Blast_Score=80, Evalue=6e-16,
Organism=Homo sapiens, GI221139811, Length=118, Percent_Identity=44.9152542372881, Blast_Score=80, Evalue=6e-16,
Organism=Homo sapiens, GI256017251, Length=118, Percent_Identity=44.9152542372881, Blast_Score=80, Evalue=6e-16,
Organism=Homo sapiens, GI310129267, Length=118, Percent_Identity=44.9152542372881, Blast_Score=80, Evalue=7e-16,
Organism=Homo sapiens, GI310120620, Length=118, Percent_Identity=44.9152542372881, Blast_Score=80, Evalue=7e-16,
Organism=Homo sapiens, GI6631100, Length=142, Percent_Identity=42.2535211267606, Blast_Score=79, Evalue=1e-15,
Organism=Homo sapiens, GI150418007, Length=127, Percent_Identity=44.0944881889764, Blast_Score=79, Evalue=2e-15,
Organism=Homo sapiens, GI310127646, Length=140, Percent_Identity=39.2857142857143, Blast_Score=79, Evalue=2e-15,
Organism=Homo sapiens, GI310114371, Length=140, Percent_Identity=39.2857142857143, Blast_Score=79, Evalue=2e-15,
Organism=Homo sapiens, GI310119221, Length=140, Percent_Identity=39.2857142857143, Blast_Score=79, Evalue=2e-15,
Organism=Homo sapiens, GI310110104, Length=118, Percent_Identity=44.0677966101695, Blast_Score=78, Evalue=3e-15,
Organism=Homo sapiens, GI178057341, Length=118, Percent_Identity=44.0677966101695, Blast_Score=77, Evalue=5e-15,
Organism=Homo sapiens, GI22547212, Length=153, Percent_Identity=40.5228758169935, Blast_Score=75, Evalue=2e-14,
Organism=Homo sapiens, GI7657473, Length=153, Percent_Identity=40.5228758169935, Blast_Score=75, Evalue=2e-14,
Organism=Homo sapiens, GI209417936, Length=118, Percent_Identity=44.0677966101695, Blast_Score=75, Evalue=2e-14,
Organism=Homo sapiens, GI30102944, Length=118, Percent_Identity=44.0677966101695, Blast_Score=75, Evalue=2e-14,
Organism=Homo sapiens, GI219842199, Length=118, Percent_Identity=44.0677966101695, Blast_Score=75, Evalue=2e-14,
Organism=Homo sapiens, GI303305009, Length=153, Percent_Identity=37.2549019607843, Blast_Score=75, Evalue=2e-14,
Organism=Homo sapiens, GI20911035, Length=126, Percent_Identity=38.0952380952381, Blast_Score=74, Evalue=3e-14,
Organism=Homo sapiens, GI4505991, Length=119, Percent_Identity=47.0588235294118, Blast_Score=74, Evalue=3e-14,
Organism=Homo sapiens, GI5174637, Length=140, Percent_Identity=39.2857142857143, Blast_Score=74, Evalue=3e-14,
Organism=Homo sapiens, GI45439316, Length=140, Percent_Identity=39.2857142857143, Blast_Score=74, Evalue=4e-14,
Organism=Homo sapiens, GI5031987, Length=128, Percent_Identity=42.1875, Blast_Score=73, Evalue=8e-14,
Organism=Homo sapiens, GI22547215, Length=135, Percent_Identity=42.2222222222222, Blast_Score=72, Evalue=2e-13,
Organism=Homo sapiens, GI169164185, Length=143, Percent_Identity=37.0629370629371, Blast_Score=71, Evalue=3e-13,
Organism=Homo sapiens, GI169163623, Length=143, Percent_Identity=37.0629370629371, Blast_Score=71, Evalue=3e-13,
Organism=Homo sapiens, GI169214050, Length=140, Percent_Identity=38.5714285714286, Blast_Score=71, Evalue=3e-13,
Organism=Homo sapiens, GI239751853, Length=140, Percent_Identity=38.5714285714286, Blast_Score=71, Evalue=4e-13,
Organism=Homo sapiens, GI113428306, Length=140, Percent_Identity=38.5714285714286, Blast_Score=71, Evalue=4e-13,
Organism=Homo sapiens, GI310127745, Length=138, Percent_Identity=40.5797101449275, Blast_Score=71, Evalue=4e-13,
Organism=Homo sapiens, GI310119290, Length=138, Percent_Identity=40.5797101449275, Blast_Score=71, Evalue=4e-13,
Organism=Homo sapiens, GI310114540, Length=138, Percent_Identity=40.5797101449275, Blast_Score=71, Evalue=4e-13,
Organism=Homo sapiens, GI169163387, Length=143, Percent_Identity=37.0629370629371, Blast_Score=70, Evalue=4e-13,
Organism=Homo sapiens, GI310132444, Length=143, Percent_Identity=38.4615384615385, Blast_Score=70, Evalue=4e-13,
Organism=Homo sapiens, GI310113152, Length=143, Percent_Identity=38.4615384615385, Blast_Score=70, Evalue=4e-13,
Organism=Homo sapiens, GI5454154, Length=131, Percent_Identity=33.587786259542, Blast_Score=68, Evalue=3e-12,
Organism=Escherichia coli, GI1789763, Length=133, Percent_Identity=37.593984962406, Blast_Score=72, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17539496, Length=138, Percent_Identity=47.1014492753623, Blast_Score=116, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71997782, Length=125, Percent_Identity=42.4, Blast_Score=93, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71980590, Length=121, Percent_Identity=40.495867768595, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17551282, Length=137, Percent_Identity=43.0656934306569, Blast_Score=88, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17551284, Length=137, Percent_Identity=43.0656934306569, Blast_Score=88, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71980594, Length=121, Percent_Identity=40.495867768595, Blast_Score=88, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17506307, Length=143, Percent_Identity=41.2587412587413, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17532641, Length=113, Percent_Identity=39.8230088495575, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17506311, Length=115, Percent_Identity=40.8695652173913, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17539498, Length=127, Percent_Identity=41.7322834645669, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17552780, Length=137, Percent_Identity=40.1459854014599, Blast_Score=76, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17559074, Length=139, Percent_Identity=41.0071942446043, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17552782, Length=138, Percent_Identity=37.6811594202899, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17565860, Length=139, Percent_Identity=38.8489208633094, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17552784, Length=146, Percent_Identity=36.986301369863, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17566266, Length=128, Percent_Identity=41.40625, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI32563659, Length=143, Percent_Identity=31.4685314685315, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17532643, Length=132, Percent_Identity=38.6363636363636, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323562, Length=142, Percent_Identity=46.4788732394366, Blast_Score=98, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6320359, Length=127, Percent_Identity=44.8818897637795, Blast_Score=84, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6321848, Length=158, Percent_Identity=39.873417721519, Blast_Score=81, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6320510, Length=129, Percent_Identity=43.4108527131783, Blast_Score=80, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6323246, Length=136, Percent_Identity=43.3823529411765, Blast_Score=79, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17986117, Length=120, Percent_Identity=48.3333333333333, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI19922376, Length=135, Percent_Identity=40.7407407407407, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI20130249, Length=131, Percent_Identity=44.2748091603053, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24650807, Length=134, Percent_Identity=44.0298507462687, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21357249, Length=111, Percent_Identity=40.5405405405405, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI45549139, Length=128, Percent_Identity=45.3125, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24652460, Length=115, Percent_Identity=35.6521739130435, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19922912, Length=122, Percent_Identity=46.7213114754098, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24664125, Length=142, Percent_Identity=39.4366197183099, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21357783, Length=142, Percent_Identity=39.4366197183099, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17647301, Length=128, Percent_Identity=41.40625, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24586038, Length=132, Percent_Identity=37.1212121212121, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17136360, Length=122, Percent_Identity=39.344262295082, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21355677, Length=128, Percent_Identity=32.8125, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015891
- InterPro:   IPR020892
- InterPro:   IPR002130 [H]

Pfam domain/function: PF00160 Pro_isomerase [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 15379; Mature: 15379

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00170 CSA_PPIASE_1 ; PS50072 CSA_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTGYIEMENGSRIEFELYPNEAPNTVANFENLANTGFYDGVIFHRVIPGFVSQGGDPTG
CCCCEEEECCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCC
TGMGGSGKTIKCETEGNPHKHEAGSLSMAHAGKDTGSSQFFIVHEPQPHLNGVHTVFGKV
CCCCCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHH
TSGLEHAKNMSNGDKMKEIRVEG
HHHHHHHHCCCCCCCEEEEEECC
>Mature Secondary Structure
MKTGYIEMENGSRIEFELYPNEAPNTVANFENLANTGFYDGVIFHRVIPGFVSQGGDPTG
CCCCEEEECCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCC
TGMGGSGKTIKCETEGNPHKHEAGSLSMAHAGKDTGSSQFFIVHEPQPHLNGVHTVFGKV
CCCCCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHH
TSGLEHAKNMSNGDKMKEIRVEG
HHHHHHHHCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 8022278; 9384377; 8755892 [H]