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The map label for this gene is trpE [H]

Identifier: 154686516

GI number: 154686516

Start: 2182374

End: 2183921

Strand: Reverse

Name: trpE [H]

Synonym: RBAM_020840

Alternate gene names: 154686516

Gene position: 2183921-2182374 (Counterclockwise)

Preceding gene: 154686517

Following gene: 154686515

Centisome position: 55.73

GC content: 46.71

Gene sequence:

>1548_bases
ATGAATTTACAATCGGATGTTTCTTTATTTTTAAAGGACAGTCTGACACACCGCACCATTCCAATCGCAGAGACTTTTAC
GGTCGACACGATCACACCCATTCAGATGGTTGAAAAACTGGACAGAAACATTACGTATCTTTTAGAAAGCAAGGATGAGT
CGTCAAGCTGGTCGAGATACTCTTTTATCGGCCTCGATCCTTTTATCACGATTAAAGAGAGTCATAACACATTCACTGCC
GCTGATCCAAACGGGCATACGCTGTTTGAAGCCGGAGAATTAAAAGCCGTGCTTGACTGGATGAATGAGAGCTTCCGCAT
TAAAACACCCGAGCTTGATATCCCATTTTCGGGAGGGGCGGTCGGTTATCTGAGCTATGACATGATTCCTTTGATTGAGC
CTTCCGTGCCAAGCCACGCGAAAGTAACAGATTTCGAAAAATGCATGCTGTTTGTCTGCCGGAAAATCATCGCGTACGAC
CACGAAACGAAACAAGTGCATTTCATTCAATATGCGGAGCTTAACGGCCGGGAGACGGAGGAAGAAAAGCGGGCCGTTTT
TAAAGAAAATTGCGCGGAGCTTCGTAAGCTTATCGGTATTTTGACAGAATCGAAAGATATGAAAGAGCCGTTTTTTTCTA
AAGAAACGTTTGACACGCCAAGTTTTGCCGCTGTGACATCAAATTATACGAAAGAAGAGTTTATGGCCGGCGTTAATAAA
CTGAAAGAATATATAAAAGCGGGAGACATTTTTCAAGGGGTGCTTTCGCAAAAGTTTGACATTCCGGTATCCAGACATTC
ATTTGAGCTGTACAGGGTGCTCCGCATCGTCAATCCTTCACCTTATATGTACTATATCAAACTGCCTGACAGAGACATTG
TCGGGAGTTCGCCTGAACGTCTGATCCGCGTGCATGACAGGCACCTGGAAATTCATCCGATTGCCGGAACGAGAAGAAGG
GGCGCCGGAAAAGAAGAGGACGAACGCCTGAAAAAAGAGCTGATGAATGACGAAAAAGAAAAAGCTGAACATTACATGCT
CGTCGATCTTGCGCGCAATGACATCGGCAGAGTTGCGGAGTACGGTTCCGTAGCCGTTCCGGAATTCACAAAGGTCGTTT
CTTTTTCACATGTGATGCACATCATCTCTGTTGTAACCGGCCGTTTAAAGCAGGGCATCCATCCCGTTGACGCGCTGATG
TCGGCATTCCCGGCAGGAACACTCACGGGCGCTCCGAAAATCCGCGCGATGCAGCTTTTAAATGAATTAGAACCGACTTC
CAGAGAAACCTACGGCGGATGTATCGCTTATATCGGATTTGACGGGTCAATTGATTCGTGTATTACCATCCGCACCATGA
GTGTCAAAAACGGTACGGCATCGGTGCAGGCGGGCGCGGGAATAGTCGCCGATTCCGTCCCGGAAGCCGAATATGAAGAA
AGCTGTAACAAAGCGGGGGCGCTTTTAAAAACGATTCAAATCGCAGAAGACATCTTTTCTGGCGGGGAGGAGAAGACGGA
TGAACAAATTTCTGCAATCGTGCGTTAA

Upstream 100 bases:

>100_bases
TTTATTCTGCCCAAAAGAAGCCTTTCTTTTGGGTTTATTTCTTATACGCAAGACTCCTCTCACTCTTCATCTCATTCTCC
TTACCGTAAGGAGGAGAATC

Downstream 100 bases:

>100_bases
TGGCCGTACGCTGAAAACCGGAGAAGCCGAAAAGCTGATGGAGATGATGATGAACGGGGACATGTCGCAGTCTGAGATCG
GCGGGATTTTATCGGTGTTA

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MNLQSDVSLFLKDSLTHRTIPIAETFTVDTITPIQMVEKLDRNITYLLESKDESSSWSRYSFIGLDPFITIKESHNTFTA
ADPNGHTLFEAGELKAVLDWMNESFRIKTPELDIPFSGGAVGYLSYDMIPLIEPSVPSHAKVTDFEKCMLFVCRKIIAYD
HETKQVHFIQYAELNGRETEEEKRAVFKENCAELRKLIGILTESKDMKEPFFSKETFDTPSFAAVTSNYTKEEFMAGVNK
LKEYIKAGDIFQGVLSQKFDIPVSRHSFELYRVLRIVNPSPYMYYIKLPDRDIVGSSPERLIRVHDRHLEIHPIAGTRRR
GAGKEEDERLKKELMNDEKEKAEHYMLVDLARNDIGRVAEYGSVAVPEFTKVVSFSHVMHIISVVTGRLKQGIHPVDALM
SAFPAGTLTGAPKIRAMQLLNELEPTSRETYGGCIAYIGFDGSIDSCITIRTMSVKNGTASVQAGAGIVADSVPEAEYEE
SCNKAGALLKTIQIAEDIFSGGEEKTDEQISAIVR

Sequences:

>Translated_515_residues
MNLQSDVSLFLKDSLTHRTIPIAETFTVDTITPIQMVEKLDRNITYLLESKDESSSWSRYSFIGLDPFITIKESHNTFTA
ADPNGHTLFEAGELKAVLDWMNESFRIKTPELDIPFSGGAVGYLSYDMIPLIEPSVPSHAKVTDFEKCMLFVCRKIIAYD
HETKQVHFIQYAELNGRETEEEKRAVFKENCAELRKLIGILTESKDMKEPFFSKETFDTPSFAAVTSNYTKEEFMAGVNK
LKEYIKAGDIFQGVLSQKFDIPVSRHSFELYRVLRIVNPSPYMYYIKLPDRDIVGSSPERLIRVHDRHLEIHPIAGTRRR
GAGKEEDERLKKELMNDEKEKAEHYMLVDLARNDIGRVAEYGSVAVPEFTKVVSFSHVMHIISVVTGRLKQGIHPVDALM
SAFPAGTLTGAPKIRAMQLLNELEPTSRETYGGCIAYIGFDGSIDSCITIRTMSVKNGTASVQAGAGIVADSVPEAEYEE
SCNKAGALLKTIQIAEDIFSGGEEKTDEQISAIVR
>Mature_515_residues
MNLQSDVSLFLKDSLTHRTIPIAETFTVDTITPIQMVEKLDRNITYLLESKDESSSWSRYSFIGLDPFITIKESHNTFTA
ADPNGHTLFEAGELKAVLDWMNESFRIKTPELDIPFSGGAVGYLSYDMIPLIEPSVPSHAKVTDFEKCMLFVCRKIIAYD
HETKQVHFIQYAELNGRETEEEKRAVFKENCAELRKLIGILTESKDMKEPFFSKETFDTPSFAAVTSNYTKEEFMAGVNK
LKEYIKAGDIFQGVLSQKFDIPVSRHSFELYRVLRIVNPSPYMYYIKLPDRDIVGSSPERLIRVHDRHLEIHPIAGTRRR
GAGKEEDERLKKELMNDEKEKAEHYMLVDLARNDIGRVAEYGSVAVPEFTKVVSFSHVMHIISVVTGRLKQGIHPVDALM
SAFPAGTLTGAPKIRAMQLLNELEPTSRETYGGCIAYIGFDGSIDSCITIRTMSVKNGTASVQAGAGIVADSVPEAEYEE
SCNKAGALLKTIQIAEDIFSGGEEKTDEQISAIVR

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=417, Percent_Identity=37.410071942446, Blast_Score=242, Evalue=5e-65,
Organism=Escherichia coli, GI1788114, Length=449, Percent_Identity=31.6258351893096, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI87082077, Length=221, Percent_Identity=27.6018099547511, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1786809, Length=214, Percent_Identity=28.0373831775701, Blast_Score=92, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6320935, Length=464, Percent_Identity=39.0086206896552, Blast_Score=293, Evalue=6e-80,
Organism=Saccharomyces cerevisiae, GI6324361, Length=405, Percent_Identity=24.4444444444444, Blast_Score=101, Evalue=3e-22,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 57812; Mature: 57812

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLQSDVSLFLKDSLTHRTIPIAETFTVDTITPIQMVEKLDRNITYLLESKDESSSWSRY
CCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHCCCEEEEEECCCCCCCCCEE
SFIGLDPFITIKESHNTFTAADPNGHTLFEAGELKAVLDWMNESFRIKTPELDIPFSGGA
EEECCCCEEEEEECCCEEEECCCCCCEEEECCHHHHHHHHCCCCEEEECCCCCCCCCCCE
VGYLSYDMIPLIEPSVPSHAKVTDFEKCMLFVCRKIIAYDHETKQVHFIQYAELNGRETE
EEEEEECCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCH
EEKRAVFKENCAELRKLIGILTESKDMKEPFFSKETFDTPSFAAVTSNYTKEEFMAGVNK
HHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHH
LKEYIKAGDIFQGVLSQKFDIPVSRHSFELYRVLRIVNPSPYMYYIKLPDRDIVGSSPER
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHH
LIRVHDRHLEIHPIAGTRRRGAGKEEDERLKKELMNDEKEKAEHYMLVDLARNDIGRVAE
HHHHHCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHEEEEEHHHHHHHHHHH
YGSVAVPEFTKVVSFSHVMHIISVVTGRLKQGIHPVDALMSAFPAGTLTGAPKIRAMQLL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHH
NELEPTSRETYGGCIAYIGFDGSIDSCITIRTMSVKNGTASVQAGAGIVADSVPEAEYEE
HHCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEEECCCCCCEECCCCEEECCCCCHHHHH
SCNKAGALLKTIQIAEDIFSGGEEKTDEQISAIVR
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MNLQSDVSLFLKDSLTHRTIPIAETFTVDTITPIQMVEKLDRNITYLLESKDESSSWSRY
CCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHCCCEEEEEECCCCCCCCCEE
SFIGLDPFITIKESHNTFTAADPNGHTLFEAGELKAVLDWMNESFRIKTPELDIPFSGGA
EEECCCCEEEEEECCCEEEECCCCCCEEEECCHHHHHHHHCCCCEEEECCCCCCCCCCCE
VGYLSYDMIPLIEPSVPSHAKVTDFEKCMLFVCRKIIAYDHETKQVHFIQYAELNGRETE
EEEEEECCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCH
EEKRAVFKENCAELRKLIGILTESKDMKEPFFSKETFDTPSFAAVTSNYTKEEFMAGVNK
HHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHH
LKEYIKAGDIFQGVLSQKFDIPVSRHSFELYRVLRIVNPSPYMYYIKLPDRDIVGSSPER
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHH
LIRVHDRHLEIHPIAGTRRRGAGKEEDERLKKELMNDEKEKAEHYMLVDLARNDIGRVAE
HHHHHCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHEEEEEHHHHHHHHHHH
YGSVAVPEFTKVVSFSHVMHIISVVTGRLKQGIHPVDALMSAFPAGTLTGAPKIRAMQLL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHH
NELEPTSRETYGGCIAYIGFDGSIDSCITIRTMSVKNGTASVQAGAGIVADSVPEAEYEE
HHCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEEECCCCCCEECCCCEEECCCCCHHHHH
SCNKAGALLKTIQIAEDIFSGGEEKTDEQISAIVR
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3924737; 6425119; 9384377; 6436812; 2422155 [H]