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The map label for this gene is trpF

Identifier: 154686513

GI number: 154686513

Start: 2179983

End: 2180636

Strand: Reverse

Name: trpF

Synonym: RBAM_020810

Alternate gene names: 154686513

Gene position: 2180636-2179983 (Counterclockwise)

Preceding gene: 154686514

Following gene: 154686512

Centisome position: 55.65

GC content: 49.39

Gene sequence:

>654_bases
ATGAAAAAGCCTGAGGTAAAGTACTGCGGAATACGATCGAAGCGTGATTACGAACTTGCGGCAGCTTCAGGAGCGGATTA
TCTGGGATTTATTTTTGCCGCAAGCAAACGGAGAGTAACGCCGGGTGAGGTCAGGAAATGGAAAGAGAAGGTCCTGACAG
ACAAGAAACATGTCGGTGTATTTGTAAATGAAACGATAGAAAACATCGCGCAAATCGCCTCTGATCTGGCACTTGACGTC
ATCCAGCTTCACGGTGATGAATCGCCTGAGGATGCCCGCCGCCTCCGTCCGCTTGTGCGCTCGGAAATATGGAAAGCGCT
CCATCACGGGGAAGGGACGCTTCGTCAGATGGCTCAATTTGCACCTGCTGTCGACGGCTATGTCATTGATTCTTCAGTAA
AAGGAATGAGAGGCGGCACGGGAATCGCGTTTTCATGGGCTCGTGTCCCCTTGTACCGAAAGCAGGCTCAAAATGCAAAC
AAGCGCTGTTTTATCGCGGGCGGCGTTAATCCTGAAACGATTACCGGCTTACTGAGATCGCGTCCGTGCGGTATAGATCT
TGCCAGCGGAATTGAAAAAAACGGACAAAAGGATCAAACACTGATCCGGCTTTTAGAAGAAAGGATGAACCACTATGTAT
CCATATCCAAATGA

Upstream 100 bases:

>100_bases
GTCAACAAGCACGGGGCGCAGGCTGTATTGGTCGGAGAATCCTTAATGAGAGAGGATTCGCAGCAAAAGGCGATCCGCGG
ACTGTTCGGGGAGTGACGAT

Downstream 100 bases:

>100_bases
AATCGGAAGATACGGCGAATTCGGCGGCAAATTTGTCCCTGAAACATTGATGCGGCCCCTTGAGGAAATCGAAACGGCTT
TCAAAGAATTAAAGAATGAT

Product: N-(5'-phosphoribosyl)anthranilate isomerase

Products: NA

Alternate protein names: PRAI

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MKKPEVKYCGIRSKRDYELAAASGADYLGFIFAASKRRVTPGEVRKWKEKVLTDKKHVGVFVNETIENIAQIASDLALDV
IQLHGDESPEDARRLRPLVRSEIWKALHHGEGTLRQMAQFAPAVDGYVIDSSVKGMRGGTGIAFSWARVPLYRKQAQNAN
KRCFIAGGVNPETITGLLRSRPCGIDLASGIEKNGQKDQTLIRLLEERMNHYVSISK

Sequences:

>Translated_217_residues
MKKPEVKYCGIRSKRDYELAAASGADYLGFIFAASKRRVTPGEVRKWKEKVLTDKKHVGVFVNETIENIAQIASDLALDV
IQLHGDESPEDARRLRPLVRSEIWKALHHGEGTLRQMAQFAPAVDGYVIDSSVKGMRGGTGIAFSWARVPLYRKQAQNAN
KRCFIAGGVNPETITGLLRSRPCGIDLASGIEKNGQKDQTLIRLLEERMNHYVSISK
>Mature_217_residues
MKKPEVKYCGIRSKRDYELAAASGADYLGFIFAASKRRVTPGEVRKWKEKVLTDKKHVGVFVNETIENIAQIASDLALDV
IQLHGDESPEDARRLRPLVRSEIWKALHHGEGTLRQMAQFAPAVDGYVIDSSVKGMRGGTGIAFSWARVPLYRKQAQNAN
KRCFIAGGVNPETITGLLRSRPCGIDLASGIEKNGQKDQTLIRLLEERMNHYVSISK

Specific function: Bifunctional Enzyme That Catalyzes Two Sequential Steps Of Tryptophan Biosynthetic Pathway. The First Reaction Is Catalyzed By The Isomerase, Coded By The Trpf Domain; The Second Reaction Is Catalyzed By The Synthase, Coded By The Trpc Domain. [C]

COG id: COG0135

COG function: function code E; Phosphoribosylanthranilate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpF family

Homologues:

Organism=Escherichia coli, GI87081863, Length=212, Percent_Identity=30.188679245283, Blast_Score=70, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6320210, Length=211, Percent_Identity=30.8056872037915, Blast_Score=82, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRPF_BACA2 (A7Z617)

Other databases:

- EMBL:   CP000560
- RefSeq:   YP_001421674.1
- ProteinModelPortal:   A7Z617
- SMR:   A7Z617
- STRING:   A7Z617
- EnsemblBacteria:   EBBACT00000005659
- GeneID:   5460945
- GenomeReviews:   CP000560_GR
- KEGG:   bay:RBAM_020810
- eggNOG:   COG0135
- GeneTree:   EBGT00050000002322
- HOGENOM:   HBG554803
- OMA:   VKGMRGG
- ProtClustDB:   PRK01222
- BioCyc:   BAMY326423:RBAM_020810-MONOMER
- HAMAP:   MF_00135_B
- InterPro:   IPR013785
- InterPro:   IPR001240
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF00697 PRAI; SSF51366 RibP_bind_barrel

EC number: =5.3.1.24

Molecular weight: Translated: 24178; Mature: 24178

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPEVKYCGIRSKRDYELAAASGADYLGFIFAASKRRVTPGEVRKWKEKVLTDKKHVGV
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHCCH
FVNETIENIAQIASDLALDVIQLHGDESPEDARRLRPLVRSEIWKALHHGEGTLRQMAQF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
APAVDGYVIDSSVKGMRGGTGIAFSWARVPLYRKQAQNANKRCFIAGGVNPETITGLLRS
CCCCCCEEECCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHC
RPCGIDLASGIEKNGQKDQTLIRLLEERMNHYVSISK
CCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHEECCC
>Mature Secondary Structure
MKKPEVKYCGIRSKRDYELAAASGADYLGFIFAASKRRVTPGEVRKWKEKVLTDKKHVGV
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHCCH
FVNETIENIAQIASDLALDVIQLHGDESPEDARRLRPLVRSEIWKALHHGEGTLRQMAQF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
APAVDGYVIDSSVKGMRGGTGIAFSWARVPLYRKQAQNANKRCFIAGGVNPETITGLLRS
CCCCCCEEECCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHC
RPCGIDLASGIEKNGQKDQTLIRLLEERMNHYVSISK
CCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA