Click here to switch to the map view.

The map label for this gene is thyA [H]

Identifier: 154686429

GI number: 154686429

Start: 2103436

End: 2104230

Strand: Reverse

Name: thyA [H]

Synonym: RBAM_019970

Alternate gene names: 154686429

Gene position: 2104230-2103436 (Counterclockwise)

Preceding gene: 154686430

Following gene: 154686428

Centisome position: 53.7

GC content: 47.8

Gene sequence:

>795_bases
ATGAAACAATATAAAGATTTGTGCCGTCATGTGTTAGAAAACGGAGAAAAAAAAGGAGACCGTACGGGAACGGGTACGAT
CAGCACGTTCGGCTACCAAATGCGGTTTAACCTTCAGGAAGGTTTCCCGATGCTTACGACGAAAAAGCTCCATTTTAAAT
CCATCGCGCATGAGCTGCTTTGGTTTTTAAAAGGAGATACGAATGTCCGCTACCTTCAGGAAAACGGAGTCCGCATCTGG
AACGAATGGGCGGATGAAAACGGAGAACTCGGACCGGTTTACGGTTCTCAATGGCGTTCATGGCGCGGAGCGGACGGCGA
GACCATTGATCAAATCTCCCGACTGATTCATGACATCAAGACAAACCCGAATTCCAGACGTTTAATCGTCAGCGCCTGGA
ATGTCGGCGAAATTGATCATATGGCGCTTCCGCCTTGCCACTGCCTGTTTCAGTTTTATGTCGCGGACGGAAAACTCTCG
TGTCAGCTTTATCAGCGCTCAGCGGATGTCTTTCTCGGCGTGCCGTTTAATATTGCGTCTTATGCGCTTTTAACAATGAT
GATCGCGCATGTGACGGGGCTTGAACCGGGTGAGTTTGTTCATACATTCGGAGACGTCCACATTTACCAAAACCATGTGG
AGCAGGTGAATTTGCAGCTGACAAGGGATGTCCGTCCGCTGCCGAAACTGCGGTTTGCGCGAAATGTTGATTCTATTTTT
GATTTTACGTTTGAAGACTTTATCATTGAGGATTATGACCCGCATCCGCACATAAAAGGAGCTGTCAGCGTATGA

Upstream 100 bases:

>100_bases
ATAGCGAGAAGAAATAAAAAAATCATTTCTGAGAAATTTTGCAGAAATGATTTTTTATTGTGTTACACTACTAAAAGACT
ACTTTAAAAGGATGAAAAAA

Downstream 100 bases:

>100_bases
TTTCGTTTATTTTTGCGATGGATGAAAACCGGCTGATCGGAAAAGATAACGATCTGCCTTGGCACCTTCCCGATGATCTC
GCATATTTCAAAAAAGTGAC

Product: thymidylate synthase

Products: NA

Alternate protein names: TS 2; TSase 2; Thymidylate synthase B; TS B; TSase B [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKQYKDLCRHVLENGEKKGDRTGTGTISTFGYQMRFNLQEGFPMLTTKKLHFKSIAHELLWFLKGDTNVRYLQENGVRIW
NEWADENGELGPVYGSQWRSWRGADGETIDQISRLIHDIKTNPNSRRLIVSAWNVGEIDHMALPPCHCLFQFYVADGKLS
CQLYQRSADVFLGVPFNIASYALLTMMIAHVTGLEPGEFVHTFGDVHIYQNHVEQVNLQLTRDVRPLPKLRFARNVDSIF
DFTFEDFIIEDYDPHPHIKGAVSV

Sequences:

>Translated_264_residues
MKQYKDLCRHVLENGEKKGDRTGTGTISTFGYQMRFNLQEGFPMLTTKKLHFKSIAHELLWFLKGDTNVRYLQENGVRIW
NEWADENGELGPVYGSQWRSWRGADGETIDQISRLIHDIKTNPNSRRLIVSAWNVGEIDHMALPPCHCLFQFYVADGKLS
CQLYQRSADVFLGVPFNIASYALLTMMIAHVTGLEPGEFVHTFGDVHIYQNHVEQVNLQLTRDVRPLPKLRFARNVDSIF
DFTFEDFIIEDYDPHPHIKGAVSV
>Mature_264_residues
MKQYKDLCRHVLENGEKKGDRTGTGTISTFGYQMRFNLQEGFPMLTTKKLHFKSIAHELLWFLKGDTNVRYLQENGVRIW
NEWADENGELGPVYGSQWRSWRGADGETIDQISRLIHDIKTNPNSRRLIVSAWNVGEIDHMALPPCHCLFQFYVADGKLS
CQLYQRSADVFLGVPFNIASYALLTMMIAHVTGLEPGEFVHTFGDVHIYQNHVEQVNLQLTRDVRPLPKLRFARNVDSIF
DFTFEDFIIEDYDPHPHIKGAVSV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=283, Percent_Identity=53.356890459364, Blast_Score=309, Evalue=2e-84,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=65.9090909090909, Blast_Score=378, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI71993377, Length=283, Percent_Identity=46.9964664310954, Blast_Score=272, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6324648, Length=292, Percent_Identity=43.1506849315069, Blast_Score=228, Evalue=7e-61,
Organism=Drosophila melanogaster, GI17137556, Length=284, Percent_Identity=54.9295774647887, Blast_Score=309, Evalue=1e-84,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 30433; Mature: 30433

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYKDLCRHVLENGEKKGDRTGTGTISTFGYQMRFNLQEGFPMLTTKKLHFKSIAHELL
CCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCEEECHHHHHHHHHHHH
WFLKGDTNVRYLQENGVRIWNEWADENGELGPVYGSQWRSWRGADGETIDQISRLIHDIK
HHHCCCCCEEEEECCCCEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TNPNSRRLIVSAWNVGEIDHMALPPCHCLFQFYVADGKLSCQLYQRSADVFLGVPFNIAS
CCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEECCHHHHH
YALLTMMIAHVTGLEPGEFVHTFGDVHIYQNHVEQVNLQLTRDVRPLPKLRFARNVDSIF
HHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHEEEEEECCCCCCHHHHHHHCHHHHH
DFTFEDFIIEDYDPHPHIKGAVSV
HHHHHHHEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MKQYKDLCRHVLENGEKKGDRTGTGTISTFGYQMRFNLQEGFPMLTTKKLHFKSIAHELL
CCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCEEECHHHHHHHHHHHH
WFLKGDTNVRYLQENGVRIWNEWADENGELGPVYGSQWRSWRGADGETIDQISRLIHDIK
HHHCCCCCEEEEECCCCEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TNPNSRRLIVSAWNVGEIDHMALPPCHCLFQFYVADGKLSCQLYQRSADVFLGVPFNIAS
CCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEECCHHHHH
YALLTMMIAHVTGLEPGEFVHTFGDVHIYQNHVEQVNLQLTRDVRPLPKLRFARNVDSIF
HHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHEEEEEECCCCCCHHHHHHHCHHHHH
DFTFEDFIIEDYDPHPHIKGAVSV
HHHHHHHEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA