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The map label for this gene is dfrA [H]

Identifier: 154686428

GI number: 154686428

Start: 2102954

End: 2103439

Strand: Reverse

Name: dfrA [H]

Synonym: RBAM_019960

Alternate gene names: 154686428

Gene position: 2103439-2102954 (Counterclockwise)

Preceding gene: 154686429

Following gene: 154686427

Centisome position: 53.68

GC content: 46.5

Gene sequence:

>486_bases
ATGATTTCGTTTATTTTTGCGATGGATGAAAACCGGCTGATCGGAAAAGATAACGATCTGCCTTGGCACCTTCCCGATGA
TCTCGCATATTTCAAAAAAGTGACGACAGGCCACACCATTGTAATGGGGCGGAAAACGTTTGAATCAATCGGACGCCCGC
TTCCGAACCGGCGTAACATCGTTGTAACCTCACGGGACGAATCTTCATTCCCGGGCTGTATTACCGCTGACTCGGCTGAG
GAAGTGCTCAAGCTGATCCCGGCGGATGAAGAATGCTTTGTAATCGGCGGCGCCCAGCTGTACAGTGCGCTCTTTCCTTA
CGCGGACAGACTCTACATGACAAAAATTCACCATGTCTTTGAAGGAGACCGTTTTTTTCCGGAATTTAATGAAGCTGAAT
GGGAATTGACATCCCGCAAACAGGGAGTAAAAGATGAAAAAAACCCTTACGATTATGAGTACCTTGTATACGAGAAAAAG
AATTGA

Upstream 100 bases:

>100_bases
GGTTTGCGCGAAATGTTGATTCTATTTTTGATTTTACGTTTGAAGACTTTATCATTGAGGATTATGACCCGCATCCGCAC
ATAAAAGGAGCTGTCAGCGT

Downstream 100 bases:

>100_bases
GTTTAAGCAGGGGGACAAGAATTGATTCGTTACGGCTGCCTTGTGATGTACGTTGTATTTATGTTCATGAAAAATATTCA
GGAATATATCAATCAAAAAG

Product: DfrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MISFIFAMDENRLIGKDNDLPWHLPDDLAYFKKVTTGHTIVMGRKTFESIGRPLPNRRNIVVTSRDESSFPGCITADSAE
EVLKLIPADEECFVIGGAQLYSALFPYADRLYMTKIHHVFEGDRFFPEFNEAEWELTSRKQGVKDEKNPYDYEYLVYEKK
N

Sequences:

>Translated_161_residues
MISFIFAMDENRLIGKDNDLPWHLPDDLAYFKKVTTGHTIVMGRKTFESIGRPLPNRRNIVVTSRDESSFPGCITADSAE
EVLKLIPADEECFVIGGAQLYSALFPYADRLYMTKIHHVFEGDRFFPEFNEAEWELTSRKQGVKDEKNPYDYEYLVYEKK
N
>Mature_161_residues
MISFIFAMDENRLIGKDNDLPWHLPDDLAYFKKVTTGHTIVMGRKTFESIGRPLPNRRNIVVTSRDESSFPGCITADSAE
EVLKLIPADEECFVIGGAQLYSALFPYADRLYMTKIHHVFEGDRFFPEFNEAEWELTSRKQGVKDEKNPYDYEYLVYEKK
N

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=183, Percent_Identity=30.6010928961749, Blast_Score=74, Evalue=5e-14,
Organism=Homo sapiens, GI28827793, Length=182, Percent_Identity=29.6703296703297, Blast_Score=64, Evalue=5e-11,
Organism=Escherichia coli, GI1786233, Length=164, Percent_Identity=37.8048780487805, Blast_Score=120, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17505899, Length=141, Percent_Identity=32.6241134751773, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6324810, Length=137, Percent_Identity=29.1970802919708, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI116008026, Length=177, Percent_Identity=33.8983050847458, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24647458, Length=177, Percent_Identity=33.8983050847458, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18697; Mature: 18697

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISFIFAMDENRLIGKDNDLPWHLPDDLAYFKKVTTGHTIVMGRKTFESIGRPLPNRRNI
CEEEEEEECCCEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCCEE
VVTSRDESSFPGCITADSAEEVLKLIPADEECFVIGGAQLYSALFPYADRLYMTKIHHVF
EEEECCCCCCCCEEECCCHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
EGDRFFPEFNEAEWELTSRKQGVKDEKNPYDYEYLVYEKKN
CCCCCCCCCCCCCEEECHHCCCCCCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MISFIFAMDENRLIGKDNDLPWHLPDDLAYFKKVTTGHTIVMGRKTFESIGRPLPNRRNI
CEEEEEEECCCEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCCEE
VVTSRDESSFPGCITADSAEEVLKLIPADEECFVIGGAQLYSALFPYADRLYMTKIHHVF
EEEECCCCCCCCEEECCCHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
EGDRFFPEFNEAEWELTSRKQGVKDEKNPYDYEYLVYEKKN
CCCCCCCCCCCCCEEECHHCCCCCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840350; 9384377 [H]